This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sidorova, J Articles by Breeden, L Search for Related Content PubMed PubMed Citation Articles by Sidorova, J Articles by Breeden, L

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 February; 13(2): 1069-1077

Analysis of the SWI4/SWI6 protein complex, which directs G1/S-specific transcription in Saccharomyces cerevisiae.

Fred Hutchinson Cancer Research Center, Seattle, Washington 98104-2092.

ABSTRACT

SWI4 and SWI6 play a crucial role in START-specific transcription in Saccharomyces cerevisiae. SWI4 and SWI6 form a specific complex on the SCB (SWI4/6-dependent cell cycle box) sequences which have been found in the promoters of HO and G1 cyclin genes. Overproduction of SWI4 eliminates the SWI6 dependency of HO transcription in vivo and results in a new SWI6-independent, SCB-specific complex in vitro, which is heterogeneous and reacts with SWI4 antibodies. The C terminus of SWI4 is not required for SWI6-independent binding of SWI4 to SCB sequences, but it is necessary and sufficient for association with SWI6. Both SWI4 and SWI6 contain two copies of a 33-amino-acid TPLH repeat, which has been implicated in protein-protein interactions in other proteins. These repeats are not required for the SWI4-SWI6 association. Alanine substitutions in both TPLH repeats of SWI6 reduce its activity but do not affect the stability of the protein or its association with SWI4. However, these mutations reduce the ability of the SWI4/6 complex to bind DNA. Deletion of the lucine zipper motif in SWI6 also allows SWI4/6 complex formation, but it eliminates the DNA-binding ability of the SWI4/6 complex. This indicates that the integrity of two different regions of SWI6 is required for DNA binding by the SWI4/6 complex. From these data, we propose that the sequence-specific DNA-binding domain resides in SWI4 but that SWI6 controls the accessibility of this domain in the SWI4/6 complex.

This article has been cited by other articles:

• Kim, K.-Y., Truman, A. W., Levin, D. E. (2008). Yeast Mpk1 Mitogen-Activated Protein Kinase Activates Transcription through Swi4/Swi6 by a Noncatalytic Mechanism That Requires Upstream Signal. Mol. Cell. Biol. 28: 2579-2589 [Abstract] [Full Text]  
• Braun, K. A., Breeden, L. L. (2007). Nascent Transcription of MCM2-7 Is Important for Nuclear Localization of the Minichromosome Maintenance Complex in G1. Mol. Biol. Cell 18: 1447-1456 [Abstract] [Full Text]  
• Jiang, Y. (2006). Regulation of the Cell Cycle by Protein Phosphatase 2A in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 70: 440-449 [Abstract] [Full Text]  
• Levin, D. E. (2005). Cell Wall Integrity Signaling in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 69: 262-291 [Abstract] [Full Text]  
• Flick, K., Wittenberg, C. (2005). Multiple Pathways for Suppression of Mutants Affecting G1-Specific Transcription in Saccharomyces cerevisiae. Genetics 169: 37-49 [Abstract] [Full Text]  
• Sidorova, J. M., Breeden, L. L. (2002). Precocious S-Phase Entry in Budding Yeast Prolongs Replicative State and Increases Dependence Upon Rad53 for Viability. Genetics 160: 123-136 [Abstract] [Full Text]  
• Baetz, K., Moffat, J., Haynes, J., Chang, M., Andrews, B. (2001). Transcriptional Coregulation by the Cell Integrity Mitogen-Activated Protein Kinase Slt2 and the Cell Cycle Regulator Swi4. Mol. Cell. Biol. 21: 6515-6528 [Abstract] [Full Text]  
• Jeffries, S., Capobianco, A. J. (2000). Neoplastic Transformation by Notch Requires Nuclear Localization. Mol. Cell. Biol. 20: 3928-3941 [Abstract] [Full Text]  
• Macpherson, N., Measday, V., Moore, L., Andrews, B. (2000). A Yeast taf17 Mutant Requires the Swi6 Transcriptional Activator for Viability and Shows Defects in Cell Cycle-Regulated Transcription. Genetics 154: 1561-1576 [Abstract] [Full Text]  
• Wijnen, H., Futcher, B. (1999). Genetic Analysis of the Shared Role of CLN3 and BCK2 at the G1-S Transition in Saccharomyces cerevisiae. Genetics 153: 1131-1143 [Abstract] [Full Text]  
• Baetz, K., Andrews, B. (1999). Regulation of Cell Cycle Transcription Factor Swi4 through Auto-Inhibition of DNA Binding. Mol. Cell. Biol. 19: 6729-6741 [Abstract] [Full Text]  
• Ho, Y., Costanzo, M., Moore, L., Kobayashi, R., Andrews, B. J. (1999). Regulation of Transcription at the Saccharomyces cerevisiae Start Transition by Stb1, a Swi6-Binding Protein. Mol. Cell. Biol. 19: 5267-5278 [Abstract] [Full Text]  
• Hache, R. J. G., Tse, R., Reich, T., Savory, J. G. A., Lefebvre, Y. A. (1999). Nucleocytoplasmic Trafficking of Steroid-free Glucocorticoid Receptor. J. Biol. Chem. 274: 1432-1439 [Abstract] [Full Text]  
• Sidorova, J., Breeden, L. (1999). The MSN1 and NHP6A Genes Suppress SWI6 Defects in Saccharomyces cerevisiae. Genetics 151: 45-55 [Abstract] [Full Text]  
• Mendenhall, M. D., Hodge, A. E. (1998). Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1191-1243 [Abstract] [Full Text]  
• Gustin, M. C., Albertyn, J., Alexander, M., Davenport, K. (1998). MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1264-1300 [Abstract] [Full Text]  
• Tabtiang, R. K., Herskowitz, I. (1998). Nuclear Proteins Nut1p and Nut2p Cooperate To Negatively Regulate a Swi4p-Dependent lacZ Reporter Gene in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 4707-4718 [Abstract] [Full Text]  
• Sidorova, J. M., Breeden, L. L. (1997). Rad53-dependent phosphorylation of Swi6 and down-regulation of CLN1 and CLN2 transcription occur in response to DNA damage in Saccharomyces cerevisiae. Genes Dev. 11: 3032-3045 [Abstract] [Full Text]  
• McInerny, C J, Partridge, J F, Mikesell, G E, Creemer, D P, Breeden, L L (1997). A novel Mcm1-dependent element in the SWI4, CLN3, CDC6, and CDC47 promoters activates M/G1-specific transcription.. Genes Dev. 11: 1277-1288 [Abstract]  
• Partridge, J. F., Mikesell, G. E., Breeden, L. L. (1997). Cell Cycle-dependent Transcription of CLN1 Involves Swi4 Binding to MCB-like Elements. J. Biol. Chem. 272: 9071-9077 [Abstract] [Full Text]  
• Madden, K., Sheu, Y.-J., Baetz, K., Andrews, B., Snyder, M. (1997). SBF Cell Cycle Regulator as a Target of the Yeast PKC-MAP Kinase Pathway. Science 275: 1781-1784 [Abstract] [Full Text]  
• Ho, Y., Mason, S., Kobayashi, R., Hoekstra, M., Andrews, B. (1997). Role of the casein kinase I isoform, Hrr25, and the cell cycle-regulatory transcription factor, SBF, in the transcriptional response to DNA damage in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 94: 581-586 [Abstract] [Full Text]  
• Kopan, R., Nye, J. S., Weintraub, H. (1994). The intracellular domain of mouse Notch: a constitutively activated repressor of myogenesis directed at the basic helix-loop-helix region of MyoD. Development 120: 2385-2396 [Abstract]  
• Zhu, Y, Takeda, T, Nasmyth, K, Jones, N (1994). pct1+, which encodes a new DNA-binding partner of p85cdc10, is required for meiosis in the fission yeast Schizosaccharomyces pombe.. Genes Dev. 8: 885-898 [Abstract]  
• Andrews, B., Mason, S. (1993). Gene expression and the cell cycle: a family affair. Science 261: 1543-1544  
• Koch, C, Moll, T, Neuberg, M, Ahorn, H, Nasmyth, K (1993). A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. Science 261: 1551-1557 [Abstract]