This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ananthan, J Articles by Voellmy, R Search for Related Content PubMed PubMed Citation Articles by Ananthan, J Articles by Voellmy, R

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 March; 13(3): 1599-1609

Synergistic activation of transcription is mediated by the N-terminal domain of Drosophila fushi tarazu homeoprotein and can occur without DNA binding by the protein.

Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101-6129.

ABSTRACT

Synergistic activation of transcription by Drosophila segmentation genes in tissue culture cells provides a model with which to study combinatorial regulation. We examined the synergistic activation of an engrailed-derived promoter by the pair-rule proteins paired (PRD) and fushi tarazu (FTZ). Synergistic activation by PRD requires regions of the homeodomain or adjacent sequences, and that by FTZ requires the first 171 residues. Surprisingly, deletion of the FTZ homeodomain does not reduce the capacity of the protein for synergistic activation, although this mutation abolishes any detectable DNA-binding activity. This finding suggests that FTZ can function through protein-protein interactions with PRD or other components of the homeoprotein transcription complex, adding a new layer of mechanisms that could underlie the functional specificities and combinatorial regulation of homeoproteins.

This article has been cited by other articles:

• Kankel, M. W., Duncan, D. M., Duncan, I. (2004). A Screen for Genes That Interact With the Drosophila Pair-Rule Segmentation Gene fushi tarazu. Genetics 168: 161-180 [Abstract] [Full Text]  
• Suzuki, T., Kawasaki, H., Yu, R. T., Ueda, H., Umesono, K. (2001). Segmentation gene product Fushi tarazu is an LXXLL motif-dependent coactivator for orphan receptor FTZ-F1. Proc. Natl. Acad. Sci. USA 10.1073/pnas.221552998v1 [Abstract] [Full Text]  
• Punzo, C., Kurata, S., Gehring, W. J. (2001). The eyeless homeodomain is dispensable for eye development in Drosophila. Genes Dev. 15: 1716-1723 [Abstract] [Full Text]  
• Sawada, K, Konishi, Y, Tominaga, M, Watanabe, Y, Hirano, J, Inoue, S, Kageyama, R, Blum, M, Tominaga, A (2000). Goosecoid suppresses cell growth and enhances neuronal differentiation of PC12 cells. J. Cell Sci. 113: 2705-2713 [Abstract]  
• Viganò, M. A., Di Rocco, G., Zappavigna, V., Mavilio, F. (1998). Definition of the Transcriptional Activation Domains of Three Human HOX Proteins Depends on the DNA-Binding Context. Mol. Cell. Biol. 18: 6201-6212 [Abstract] [Full Text]  
• Inukai, T., Inaba, T., Ikushima, S., Look, A. T. (1998). The AD1 and AD2 Transactivation Domains of E2A Are Essential for the Antiapoptotic Activity of the Chimeric Oncoprotein E2A-HLF. Mol. Cell. Biol. 18: 6035-6043 [Abstract] [Full Text]  
• Ranganayakulu, G, Elliott, D., Harvey, R., Olson, E. (1998). Divergent roles for NK-2 class homeobox genes in cardiogenesis in flies and mice. Development 125: 3037-3048 [Abstract]  
• Shiojima, I., Komuro, I., Mizuno, T., Aikawa, R., Akazawa, H., Oka, T., Yamazaki, T., Yazaki, Y. (1996). Molecular Cloning and Characterization of Human Cardiac Homeobox Gene CSX1. Circ. Res. 79: 920-929 [Abstract] [Full Text]  
• Fujioka, M, Miskiewicz, P, Raj, L, Gulledge, A., Weir, M, Goto, T (1996). Drosophila Paired regulates late even-skipped expression through a composite binding site for the paired domain and the homeodomain. Development 122: 2697-2707 [Abstract]  
• Miskiewicz, P, Morrissey, D, Lan, Y, Raj, L, Kessler, S, Fujioka, M, Goto, T, Weir, M (1996). Both the paired domain and homeodomain are required for in vivo function of Drosophila Paired. Development 122: 2709-2718 [Abstract]  
• Briata, P., Van De Werken, R., Airoldi, I., Ilengo, C., Di Blas, E., Boncinelli, E., Corte, G. (1995). Transcriptional Repression by the Human Homeobox Protein EVX1 in Transfected Mammalian Cells. J. Biol. Chem. 270: 27695-27701 [Abstract] [Full Text]  
• Chang, C P, Shen, W F, Rozenfeld, S, Lawrence, H J, Largman, C, Cleary, M L (1995). Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins.. Genes Dev. 9: 663-674 [Abstract]  
• Zappavigna, V, Sartori, D, Mavilio, F (1994). Specificity of HOX protein function depends on DNA-protein and protein-protein interactions, both mediated by the homeo domain.. Genes Dev. 8: 732-744 [Abstract]  
• Millen, K., Wurst, W, Herrup, K, Joyner, A. (1994). Abnormal embryonic cerebellar development and patterning of postnatal foliation in two mouse Engrailed-2 mutants. Development 120: 695-706 [Abstract]  
• Norris, R. A., Kern, M. J. (2001). The Identification of Prx1 Transcription Regulatory Domains Provides a Mechanism for Unequal Compensation by the Prx1 and Prx2 Loci. J. Biol. Chem. 276: 26829-26837 [Abstract] [Full Text]  
• Suzuki, T., Kawasaki, H., Yu, R. T., Ueda, H., Umesono, K. (2001). Segmentation gene product Fushi tarazu is an LXXLL motif-dependent coactivator for orphan receptor FTZ-F1. Proc. Natl. Acad. Sci. USA 98: 12403-12408 [Abstract] [Full Text]