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Mol Cell Biol. 1993 April; 13(4): 2069-2080

Pheromone-induced signal transduction in Saccharomyces cerevisiae requires the sequential function of three protein kinases.

Department of Chemistry, University of North Carolina, Chapel Hill 27599.

ABSTRACT

Protein phosphorylation plays an important role in pheromone-induced differentiation processes of haploid yeast cells. Among the components necessary for signal transduction are the STE7 and STE11 kinases and either one of the redundant FUS3 and KSS1 kinases. FUS3 and presumably KSS1 are phosphorylated and activated during pheromone induction by a STE7-dependent mechanism. Pheromone also induces the accumulation of STE7 in a hyperphosphorylated form. This modification of STE7 requires the STE11 kinase, which is proposed to act before STE7 during signal transmission. Surprisingly, STE7 hyperphosphorylation also requires a functional FUS3 (or KSS1) kinase. Using in vitro assays for FUS3 phosphorylation, we show that pheromone activates STE7 even in the absence of FUS3 and KSS1. Therefore, STE7 activation must precede modification of FUS3 (and KSS1). These findings suggest that STE7 hyperphosphorylation is a consequence of its activation but not the determining event.

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• Jeoung, D.-I., Oehlen, L. J. W. M., Cross, F. R. (1998). Cln3-Associated Kinase Activity in Saccharomyces cerevisiae Is Regulated by the Mating Factor Pathway. Mol. Cell. Biol. 18: 433-441 [Abstract] [Full Text]  
• Zhan, X L, Deschenes, R J, Guan, K L (1997). Differential regulation of FUS3 MAP kinase by tyrosine-specific phosphatases PTP2/PTP3 and dual-specificity phosphatase MSG5 in Saccharomyces cerevisiae.. Genes Dev. 11: 1690-1702 [Abstract]  
• Wassmann, K., Ammerer, G. (1997). Overexpression of the G1-cyclin Gene CLN2 Represses the Mating Pathway in Saccharomyces cerevisiae at the Level of the MEKK Ste11. J. Biol. Chem. 272: 13180-13188 [Abstract] [Full Text]  
• Stevenson, B J, Ferguson, B, De Virgilio, C, Bi, E, Pringle, J R, Ammerer, G, Sprague, G F (1995). Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae.. Genes Dev. 9: 2949-2963 [Abstract]  
• Wu, C., Whiteway, M., Thomas, D. Y., Leberer, E. (1995). Molecular Characterization of Ste20p, a Potential Mitogen-activated Protein or Extracellular Signal-regulated Kinase Kinase (MEK) Kinase Kinase from Saccharomycescerevisiae. J. Biol. Chem. 270: 15984-15992 [Abstract] [Full Text]  
• Roberts, R L, Fink, G R (1994). Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth.. Genes Dev. 8: 2974-2985 [Abstract]  
• Kranz, J E, Satterberg, B, Elion, E A (1994). The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5.. Genes Dev. 8: 313-327 [Abstract]  
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