This Article
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bueno, A
Right arrow Articles by Russell, P
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bueno, A
Right arrow Articles by Russell, P

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 April; 13(4): 2286-2297

Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis.

A Bueno and P Russell

Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037.

ABSTRACT

Cyclin B interacts with Cdc2 kinase to induce cell cycle events, particularly those of mitosis. The existence of cyclin B subtypes in several species has been known for some time, leading to speculation that key events of mitosis may be carried out by distinct functional classes of Cdc2/cyclin B. We report the discovery of cig2, a third B-type cyclin gene in Schizosaccharomyces pombe. Disruption of cig2 delays the onset of mitosis, to the degree that a cig2 null allele rescues mitotic catastrophe mutants, including those that are unable to carry out the inhibitory tyrosyl phosphorylation of Cdc2 kinase. Consistent with this, a cig2 null allele exhibits synthetic lethal interactions with cdc25ts and cdc2ts mutations. Mitotic phenotypes caused by disruption of cig2 are not reversed by increased production of Cdc13, the other fission yeast B-type cyclin that functions in mitosis. Likewise, a cdc13ts mutation is not rescued by increased gene dosage of cig2+. These data indicate that Cdc13 and Cig2 interact with Cdc2 to carry out different functions in mitosis. We suggest that some cyclin B subtypes found in other species, including humans, are also likely to have distinct, nonoverlapping functions in mitosis.


Mol Cell Biol. 1993 April; 13(4): 2286-2297




This article has been cited by other articles:

  • Malapeira, J., Moldon, A., Hidalgo, E., Smith, G. R., Nurse, P., Ayte, J. (2005). A Meiosis-Specific Cyclin Regulated by Splicing Is Required for Proper Progression through Meiosis. Mol. Cell. Biol. 25: 6330-6337 [Abstract] [Full Text]  
  • Garcia-Muse, T., Steinberg, G., Perez-Martin, J. (2004). Characterization of B-type cyclins in the smut fungus Ustilago maydis: roles in morphogenesis and pathogenicity. J. Cell Sci. 117: 487-506 [Abstract] [Full Text]  
  • Moore, J. D., Kirk, J. A., Hunt, T. (2003). Unmasking the S-Phase-Promoting Potential of Cyclin B1. Science 300: 987-990 [Abstract] [Full Text]  
  • Chua, G., Lingner, C., Frazer, C., Young, P. G. (2002). The sal3+ Gene Encodes an Importin-{beta} Implicated in the Nuclear Import of Cdc25 in Schizosaccharomyces pombe. Genetics 162: 689-703 [Abstract] [Full Text]  
  • Borgne, A., Murakami, H., Ayte, J., Nurse, P. (2002). The G1/S Cyclin Cig2p during Meiosis in Fission Yeast. Mol. Biol. Cell 13: 2080-2090 [Abstract] [Full Text]  
  • Ochotorena, I. L., Hirata, D., Kominami, K.-i., Potashkin, J., Sahin, F., Wentz-Hunter, K., Gould, K. L., Sato, K., Yoshida, Y., Vardy, L., Toda, T. (2002). Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation. J. Cell Sci. 114: 2911-2920 [Abstract] [Full Text]  
  • Kottom, T. J., Thomas, C. F. Jr., Mubarak, K. K., Leof, E. B., Limper, A. H. (2000). Pneumocystis carinii Uses a Functional Cdc13 B-Type Cyclin Complex during Its Life Cycle. Am. J. Respir. Cell Mol. Bio. 22: 722-731 [Abstract] [Full Text]  
  • Tanaka, K., Yonekawa, T., Kawasaki, Y., Kai, M., Furuya, K., Iwasaki, M., Murakami, H., Yanagida, M., Okayama, H. (2000). Fission Yeast Eso1p Is Required for Establishing Sister Chromatid Cohesion during S Phase. Mol. Cell. Biol. 20: 3459-3469 [Abstract] [Full Text]  
  • Borgne, A, Nurse, P (2000). The Spd1p S phase inhibitor can activate the DNA replication checkpoint pathway in fission yeast. J. Cell Sci. 113: 4341-4350 [Abstract]  
  • Snaith, H. A., Forsburg, S. L. (1999). Rereplication Phenomenon in Fission Yeast Requires MCM Proteins and Other S Phase Genes. Genetics 152: 839-851 [Abstract] [Full Text]  
  • Bridge, A. J., Morphew, M., Bartlett, R., Hagan, I. M. (1998). The fission yeast SPB component Cut12 links bipolar spindle formation to mitotic control. Genes Dev. 12: 927-942 [Abstract] [Full Text]  
  • Sauer, K, Knoblich, J A, Richardson, H, Lehner, C F (1995). Distinct modes of cyclin E/cdc2c kinase regulation and S-phase control in mitotic and endoreduplication cycles of Drosophila embryogenesis.. Genes Dev. 9: 1327-1339 [Abstract]  
  • Labib, K, Moreno, S, Nurse, P (1995). Interaction of cdc2 and rum1 regulates Start and S-phase in fission yeast. J. Cell Sci. 108: 3285-3294 [Abstract]  
  • Forsburg, S., Nurse, P (1994). Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit. J. Cell Sci. 107: 601-613 [Abstract]