This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Deshmukh, M Articles by Woolford, J L Search for Related Content PubMed PubMed Citation Articles by Deshmukh, M Articles by Woolford, J L, Jr

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 May; 13(5): 2835-2845

Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits.

Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213-2683.

ABSTRACT

Ribosomal protein L1 from Saccharomyces cerevisiae binds 5S rRNA and can be released from intact 60S ribosomal subunits as an L1-5S ribonucleoprotein (RNP) particle. To understand the nature of the interaction between L1 and 5S rRNA and to assess the role of L1 in ribosome assembly and function, we cloned the RPL1 gene encoding L1. We have shown that RPL1 is an essential single-copy gene. A conditional null mutant in which the only copy of RPL1 is under control of the repressible GAL1 promoter was constructed. Depletion of L1 causes instability of newly synthesized 5S rRNA in vivo. Cells depleted of L1 no longer assemble 60S ribosomal subunits, indicating that L1 is required for assembly of stable 60S ribosomal subunits but not 40S ribosomal subunits. An L1-5S RNP particle not associated with ribosomal particles was detected by coimmunoprecipitation of L1 and 5S rRNA. This pool of L1-5S RNP remained stable even upon cessation of 60S ribosomal subunit assembly by depletion of another ribosomal protein, L16. Preliminary results suggest that transcription of RPL1 is not autogenously regulated by L1.

This article has been cited by other articles:

• Decatur, W. A., Schnare, M. N. (2008). Different Mechanisms for Pseudouridine Formation in Yeast 5S and 5.8S rRNAs. Mol. Cell. Biol. 28: 3089-3100 [Abstract] [Full Text]  
• Bommakanti, A. S., Lindahl, L., Zengel, J. M. (2008). Mutation from guanine to adenine in 25S rRNA at the position equivalent to E. coli A2058 does not confer erythromycin sensitivity in Sacchromyces cerevisae. RNA 14: 460-464 [Abstract] [Full Text]  
• Zhang, J., Harnpicharnchai, P., Jakovljevic, J., Tang, L., Guo, Y., Oeffinger, M., Rout, M. P., Hiley, S. L., Hughes, T., Woolford, J. L. Jr. (2007). Assembly factors Rpf2 and Rrs1 recruit 5S rRNA and ribosomal proteins rpL5 and rpL11 into nascent ribosomes. Genes Dev. 21: 2580-2592 [Abstract] [Full Text]  
• Hellman, K. M., Ciganda, M., Brown, S. V., Li, J., Ruyechan, W., Williams, N. (2007). Two Trypanosome-Specific Proteins Are Essential Factors for 5S rRNA Abundance and Ribosomal Assembly in Trypanosoma brucei. Eukaryot Cell 6: 1766-1772 [Abstract] [Full Text]  
• Martin-Marcos, P., Hinnebusch, A. G., Tamame, M. (2007). Ribosomal Protein L33 Is Required for Ribosome Biogenesis, Subunit Joining, and Repression of GCN4 Translation. Mol. Cell. Biol. 27: 5968-5985 [Abstract] [Full Text]  
• Rosado, I. V., Kressler, D., Cruz, J. d. l. (2007). Functional analysis of Saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis. Nucleic Acids Res 0: gkm388v1-11 [Abstract] [Full Text]  
• Rosado, I. V., Dez, C., Lebaron, S., Caizergues-Ferrer, M., Henry, Y., de la Cruz, J. (2007). Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) Reveals a Low-Molecular-Mass Complex Containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p Involved in Early Steps of 60S Ribosomal Subunit Biogenesis. Mol. Cell. Biol. 27: 1207-1221 [Abstract] [Full Text]  
• Laferte, A., Favry, E., Sentenac, A., Riva, M., Carles, C., Chedin, S. (2006). The transcriptional activity of RNA polymerase I is a key determinant for the level of all ribosome components. Genes Dev. 20: 2030-2040 [Abstract] [Full Text]  
• Nariai, M., Tanaka, T., Okada, T., Shirai, C., Horigome, C., Mizuta, K. (2005). Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S rRNA in Saccharomyces cerevisiae. Nucleic Acids Res 33: 4553-4562 [Abstract] [Full Text]  
• Marygold, S. J., Coelho, C. M. A., Leevers, S. J. (2005). Genetic Analysis of RpL38 and RpL5, Two Minute Genes Located in the Centric Heterochromatin of Chromosome 2 of Drosophila melanogaster. Genetics 169: 683-695 [Abstract] [Full Text]  
• ROSADO, I. V., DE LA CRUZ, J. (2004). Npa1p is an essential trans-acting factor required for an early step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. RNA 10: 1073-1083 [Abstract] [Full Text]  
• HORSEY, E. W., JAKOVLJEVIC, J., MILES, T. D., HARNPICHARNCHAI, P., WOOLFORD, J. L. JR. (2004). Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation. RNA 10: 813-827 [Abstract] [Full Text]  
• Fingerman, I., Nagaraj, V., Norris, D., Vershon, A. K. (2003). Sfp1 Plays a Key Role in Yeast Ribosome Biogenesis. Eukaryot Cell 2: 1061-1068 [Abstract] [Full Text]  
• Mathieu, O., Yukawa, Y., Prieto, J.-L., Vaillant, I., Sugiura, M., Tourmente, S. (2003). Identification and characterization of transcription factor IIIA and ribosomal protein L5 from Arabidopsis thaliana. Nucleic Acids Res 31: 2424-2433 [Abstract] [Full Text]  
• Lee, Y., Nazar, R. N. (2003). Terminal Structure Mediates 5 S rRNA Stability and Integration during Ribosome Biogenesis. J. Biol. Chem. 278: 6635-6641 [Abstract] [Full Text]  
• Moy, T. I., Boettner, D., Rhodes, J. C., Silver, P. A., Askew, D. S. (2002). Identification of a role for Saccharomyces cerevisiae Cgr1p in pre-rRNA processing and 60S ribosome subunit synthesis. Microbiology 148: 1081-1090 [Abstract] [Full Text]  
• Smith, M. W., Meskauskas, A., Wang, P., Sergiev, P. V., Dinman, J. D. (2001). Saturation Mutagenesis of 5S rRNA in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 8264-8275 [Abstract] [Full Text]  
• van Beekvelt, C. A., de Graaff-Vincent, M., Faber, A. W., van 't Riet, J., Venema, J., Raue, H. A. (2001). All three functional domains of the large ribosomal subunit protein L25 are required for both early and late pre-rRNA processing steps in Saccharomyces cerevisiae. Nucleic Acids Res 29: 5001-5008 [Abstract] [Full Text]  
• Lin, E., Lin, S.-W., Lin, A. (2001). The participation of 5S rRNA in the co-translational formation of a eukaryotic 5S ribonucleoprotein complex. Nucleic Acids Res 29: 2510-2516 [Abstract] [Full Text]  
• Basu, U., Si, K., Warner, J. R., Maitra, U. (2001). The Saccharomyces cerevisiae TIF6 Gene Encoding Translation Initiation Factor 6 Is Required for 60S Ribosomal Subunit Biogenesis. Mol. Cell. Biol. 21: 1453-1462 [Abstract] [Full Text]  
• Briand, J.-F., Navarro, F., Gadal, O., Thuriaux, P. (2001). Cross Talk between tRNA and rRNA Synthesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 189-195 [Abstract] [Full Text]  
• Burger, F., Daugeron, M.-C., Linder, P. (2000). Dbp10p, a putative RNA helicase from Saccharomyces cerevisiae, is required for ribosome biogenesis. Nucleic Acids Res 28: 2315-2323 [Abstract] [Full Text]  
• Kressler, D., Linder, P., de la Cruz, J. (1999). Protein trans-Acting Factors Involved in Ribosome Biogenesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 7897-7912 [Full Text]  
• Kressler, D., Doere, M., Rojo, M., Linder, P. (1999). Synthetic Lethality with Conditional dbp6 Alleles Identifies Rsa1p, a Nucleoplasmic Protein Involved in the Assembly of 60S Ribosomal Subunits. Mol. Cell. Biol. 19: 8633-8645 [Abstract] [Full Text]  
• Sobel, S. G., Wolin, S. L. (1999). Two Yeast La Motif-containing Proteins Are RNA-binding Proteins that Associate with Polyribosomes. Mol. Biol. Cell 10: 3849-3862 [Abstract] [Full Text]  
• Dechampesme, A.-M., Koroleva, O., Leger-Silvestre, I., Gas, N., Camier, S. (1999). Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the Pre-rRNA Processing Pathway. J. Cell Biol. 145: 1369-1380 [Abstract] [Full Text]  
• Sanvito, F., Piatti, S., Villa, A., Bossi, M., Lucchini, G., Marchisio, P. C., Biffo, S. (1999). The beta 4 Integrin Interactor p27BBP/eIF6 Is an Essential Nuclear Matrix Protein Involved in 60S Ribosomal Subunit Assembly. J. Cell Biol. 144: 823-838 [Abstract] [Full Text]  
• Si, K., Maitra, U. (1999). The Saccharomyces cerevisiae Homologue of Mammalian Translation Initiation Factor 6 Does Not Function as a Translation Initiation Factor. Mol. Cell. Biol. 19: 1416-1426 [Abstract] [Full Text]  
• Zanchin, N. I. T., Goldfarb, D. S. (1999). Nip7p Interacts with Nop8p, an Essential Nucleolar Protein Required for 60S Ribosome Biogenesis, and the Exosome Subunit Rrp43p. Mol. Cell. Biol. 19: 1518-1525 [Abstract] [Full Text]  
• Kressler, D., de la Cruz, J., Rojo, M., Linder, P. (1998). Dbp6p Is an Essential Putative ATP-Dependent RNA Helicase Required for 60S-Ribosomal-Subunit Assembly in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 1855-1865 [Abstract] [Full Text]  
• Lee, Y., Nazar, R. N. (1997). Ribosomal 5 S rRNA Maturation in Saccharomyces cerevisiae. J. Biol. Chem. 272: 15206-15212 [Abstract] [Full Text]  
• Dick, F. A., Karamanou, S., Trumpower, B. L. (1997). QSR1, an Essential Yeast Gene with a Genetic Relationship to a Subunit of the Mitochondrial Cytochrome bc1 Complex, Codes for a 60 S Ribosomal Subunit Protein. J. Biol. Chem. 272: 13372-13379 [Abstract] [Full Text]  
• Proweller, A., Butler, J. S. (1997). Ribosome Concentration Contributes to Discrimination against Poly(A)- mRNA during Translation Initiation in Saccharomyces cerevisiae. J. Biol. Chem. 272: 6004-6010 [Abstract] [Full Text]  
• Martin-Nieto, J, Roufa, D. (1997). Functional analysis of human RPS14 null alleles. J. Cell Sci. 110: 955-963 [Abstract]  
• Lee, J. C., Turgeon, C. L., Yeh, L.-C. C. (1996). The Accessibility of Yeast Ribosomal Protein L1 as Probed by Proteolysis and Site-directed Mutagenesis Is Different in Intact 60 and 80 S Ribosome. J. Biol. Chem. 271: 7429-7434 [Abstract] [Full Text]  
• Deshmukh, M., Stark, J., Yeh, L.-C. C., Lee, J. C., Woolford, J. L. Jr. (1995). Multiple Regions of Yeast Ribosomal Protein L1 Are Important for Its Interaction with 5 S rRNA and Assembly into Ribosomes. J. Biol. Chem. 270: 30148-30156 [Abstract] [Full Text]