This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kudrycki, K Articles by Margolis, F L Search for Related Content PubMed PubMed Citation Articles by Kudrycki, K Articles by Margolis, F L

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 May; 13(5): 3002-3014

Olf-1-binding site: characterization of an olfactory neuron-specific promoter motif.

Department of Neurosciences, Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.

ABSTRACT

We report characterization of several domains within the 5' flanking region of the olfactory marker protein (OMP) gene that may participate in regulating transcription of this and other olfactory neuron-specific genes. Analysis by electrophoretic mobility shift assay and DNase I footprinting identifies two regions that contain a novel sequence motif. Interactions between this motif and nuclear proteins were detected only with nuclear protein extracts derived from olfactory neuroepithelium, and this activity is more abundant in olfactory epithelium enriched in immature neurons. We have designated a factor(s) involved in this binding as Olf-1. The Olf-1-binding motif consensus sequence was defined as TCCCC(A/T)NGGAG. Studies with transgenic mice indicate that a 0.3-kb fragment of the OMP gene containing one Olf-1 motif is sufficient for olfactory tissue-specific expression of the reporter gene. Some of the other identified sequence motifs also interact specifically with olfactory nuclear protein extracts. We propose that Olf-1 is a novel, olfactory neuron-specific trans-acting factor involved in the cell-specific expression of OMP.

This article has been cited by other articles:

• Lagergren, A., Mansson, R., Zetterblad, J., Smith, E., Basta, B., Bryder, D., Akerblad, P., Sigvardsson, M. (2007). The Cxcl12, Periostin, and Ccl9 Genes Are Direct Targets for Early B-cell Factor in OP-9 Stroma Cells. J. Biol. Chem. 282: 14454-14462 [Abstract] [Full Text]  
• Michaloski, J. S., Galante, P. A.F., Malnic, B. (2006). Identification of potential regulatory motifs in odorant receptor genes by analysis of promoter sequences. Genome Res 16: 1091-1098 [Abstract] [Full Text]  
• Croci, L., Chung, S.-H., Masserdotti, G., Gianola, S., Bizzoca, A., Gennarini, G., Corradi, A., Rossi, F., Hawkes, R., Consalez, G. G. (2006). A key role for the HLH transcription factor EBF2COE2,O/E-3 in Purkinje neuron migration and cerebellar cortical topography. Development 133: 2719-2729 [Abstract] [Full Text]  
• Ling, G., Hauer, C. R., Gronostajski, R. M., Pentecost, B. T., Ding, X. (2004). Transcriptional Regulation of Rat CYP2A3 by Nuclear Factor 1: IDENTIFICATION OF A NOVEL NFI-A ISOFORM, AND EVIDENCE FOR TISSUE-SELECTIVE INTERACTION OF NFI WITH THE CYP2A3 PROMOTER IN VIVO. J. Biol. Chem. 279: 27888-27895 [Abstract] [Full Text]  
• Wang, S. S., Lewcock, J. W., Feinstein, P., Mombaerts, P., Reed, R. R. (2004). Genetic disruptions of O/E2 and O/E3 genes reveal involvement in olfactory receptor neuron projection. Development 131: 1377-1388 [Abstract] [Full Text]  
• Merluzzi, S., Moretti, M., Altamura, S., Zwollo, P., Sigvardsson, M., Vitale, G., Pucillo, C. (2004). CD40 Stimulation Induces Pax5/BSAP and EBF Activation through a APE/Ref-1-dependent Redox Mechanism. J. Biol. Chem. 279: 1777-1786 [Abstract] [Full Text]  
• Garcia-Dominguez, M., Poquet, C., Garel, S., Charnay, P. (2003). Ebf gene function is required for coupling neuronal differentiation and cell cycle exit. Development 130: 6013-6025 [Abstract] [Full Text]  
• Hoppe, R., Frank, H., Breer, H., Strotmann, J. (2003). The Clustered Olfactory Receptor Gene Family 262: Genomic Organization, Promotor Elements, and Interacting Transcription Factors. Genome Res 13: 2674-2685 [Abstract] [Full Text]  
• Zhao, F., McCarrick-Walmsley, R., Akerblad, P., Sigvardsson, M., Kadesch, T. (2003). Inhibition of p300/CBP by Early B-Cell Factor. Mol. Cell. Biol. 23: 3837-3846 [Abstract] [Full Text]  
• Corradi, A., Croci, L., Broccoli, V., Zecchini, S., Previtali, S., Wurst, W., Amadio, S., Maggi, R., Quattrini, A., Consalez, G. G. (2003). Hypogonadotropic hypogonadism and peripheral neuropathy in Ebf2-null mice. Development 130: 401-410 [Abstract] [Full Text]  
• Liberg, D., Sigvardsson, M., Akerblad, P. (2002). The EBF/Olf/Collier Family of Transcription Factors: Regulators of Differentiation in Cells Originating from All Three Embryonal Germ Layers. Mol. Cell. Biol. 22: 8389-8397 [Full Text]  
• Akerblad, P., Lind, U., Liberg, D., Bamberg, K., Sigvardsson, M. (2002). Early B-Cell Factor (O/E-1) Is a Promoter of Adipogenesis and Involved in Control of Genes Important for Terminal Adipocyte Differentiation. Mol. Cell. Biol. 22: 8015-8025 [Abstract] [Full Text]  
• Kobayakawa, K., Hayashi, R., Morita, K., Miyamichi, K., Oka, Y., Tsuboi, A., Sakano, H. (2002). Stomatin-Related Olfactory Protein, SRO, Specifically Expressed in the Murine Olfactory Sensory Neurons. J. Neurosci. 22: 5931-5937 [Abstract] [Full Text]  
• Saur, D., Seidler, B., Paehge, H., Schusdziarra, V., Allescher, H.-D. (2002). Complex Regulation of Human Neuronal Nitric-oxide Synthase Exon 1c Gene Transcription. ESSENTIAL ROLE OF Sp AND ZNF FAMILY MEMBERS OF TRANSCRIPTION FACTORS. J. Biol. Chem. 277: 25798-25814 [Abstract] [Full Text]  
• Boeda, B., Weil, D., Petit, C. (2001). A specific promoter of the sensory cells of the inner ear defined by transgenesis. Hum Mol Genet 10: 1581-1589 [Abstract] [Full Text]  
• Pyrski, M., Xu, Z., Walters, E., Gilbert, D. J., Jenkins, N. A., Copeland, N. G., Margolis, F. L. (2001). The OMP-lacZ Transgene Mimics the Unusual Expression Pattern of OR-Z6, a New Odorant Receptor Gene on Mouse Chromosome 6: Implication for Locus-Dependent Gene Expression. J. Neurosci. 21: 4637-4648 [Abstract] [Full Text]  
• Zhou, Y, Yamamoto, M, Engel, J. (2000). GATA2 is required for the generation of V2 interneurons. Development 127: 3829-3838 [Abstract]  
• Devireddy, L. R., Jones, C. J. (2000). Olf-1, a Neuron-specific Transcription Factor, Can Activate the Herpes Simplex Virus Type 1-Infected Cell Protein 0 Promoter. J. Biol. Chem. 275: 77-81 [Abstract] [Full Text]  
• Sicard, G., Feron, F., Andrieu, J. L., Holley, A., Mackaysim, A. (1998). Generation of Neurons from a Nonneuronal Precursor in Adult Olfactory Epithelium in Vitro. Ann. N. Y. Acad. Sci. 855: 223-225 [Abstract] [Full Text]  
• Zhang, J., Ding, X. (1998). Identification and Characterization of a Novel Tissue-specific Transcriptional Activating Element in the 5'-Flanking Region of the CYP2A3 Gene Predominantly Expressed in Rat Olfactory Mucosa. J. Biol. Chem. 273: 23454-23462 [Abstract] [Full Text]  
• Malgaretti, N., Pozzoli, O., Bosetti, A., Corradi, A., Ciarmatori, S., Panigada, M., Bianchi, M. E., Martinez, S., Consalez, G. G. (1997). Mmot1, a New Helix-Loop-Helix Transcription Factor Gene Displaying a Sharp Expression Boundary in the Embryonic Mouse Brain. J. Biol. Chem. 272: 17632-17639 [Abstract] [Full Text]  
• Wang, S. S., Tsai, R. Y. L., Reed, R. R. (1997). The Characterization of the Olf-1/EBF-Like HLH Transcription Factor Family: Implications in Olfactory Gene Regulation and Neuronal Development. J. Neurosci. 17: 4149-4158 [Abstract] [Full Text]  
• Tsai, R. Y. L., Reed, R. R. (1997). Cloning and Functional Characterization of Roaz, a Zinc Finger Protein that Interacts with O/E-1 to Regulate Gene Expression: Implications for Olfactory Neuronal Development. J. Neurosci. 17: 4159-4169 [Abstract] [Full Text]  
• Frenkel, B., Capparelli, C., van Auken, M., , D. B., Bryan, J., Stein, J. L., Stein, G. S., Lian, J. B. (1997). Activity of the Osteocalcin Promoter in Skeletal Sites of Transgenic Mice and during Osteoblast Differentiation in Bone Marrow-Derived Stromal Cell Cultures: Effects of Age and Sex. Endocrinology 138: 2109-2116 [Abstract] [Full Text]  
• Davis, J. A., Reed, R. R. (1996). Role of Olf-1 and Pax-6 Transcription Factors in Neurodevelopment. J. Neurosci. 16: 5082-5094 [Abstract] [Full Text]  
• Ryba, N. J. P., Tirindelli, R. (1995). A Novel GTP-binding Protein [IMAGE]-Subunit, G[IMAGE]8, Is Expressed during Neurogenesis in the Olfactory and Vomeronasal Neuroepithelia. J. Biol. Chem. 270: 6757-6767 [Abstract] [Full Text]