This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cross, F R Articles by Blake, C M Search for Related Content PubMed PubMed Citation Articles by Cross, F R Articles by Blake, C M

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 June; 13(6): 3266-3271

The yeast Cln3 protein is an unstable activator of Cdc28.

Rockefeller University, New York, New York 10021.

ABSTRACT

The Cln3 cyclin homolog of Saccharomyces cerevisiae functions to promote cell cycle START for only a short time following its synthesis. Cln3 protein is highly unstable and is stabilized by C-terminal truncation. Cln3 binds to Cdc28, a protein kinase catalytic subunit essential for cell cycle START, and Cln3 instability requires Cdc28 activity. The long functional lifetime and the hyperactivity of C-terminally truncated Cln3 (Cln3-2) relative to those of full-length Cln3 are affected by mutations in CDC28: the functional lifetime of Cln3-2 is drastically reduced by the cdc28-13 mutation at the permissive temperature, and the cdc28-4 mutation at the permissive temperature completely blocks the function of Cln3-2 while only partially reducing the function of full-length Cln3. Thus, sequences in the C-terminal third of Cln3 might help stabilize functional Cdc28-Cln3 association, as well as decreasing the lifetime of the Cln3 protein. These and other results strongly support the idea that Cln proteins function to activate Cdc28 at START.

This article has been cited by other articles:

• Jorgensen, P., Edgington, N. P., Schneider, B. L., Rupes, I., Tyers, M., Futcher, B. (2007). The Size of the Nucleus Increases as Yeast Cells Grow. Mol. Biol. Cell 18: 3523-3532 [Abstract] [Full Text]  
• Bernstein, K. A., Bleichert, F., Bean, J. M., Cross, F. R., Baserga, S. J. (2007). Ribosome Biogenesis Is Sensed at the Start Cell Cycle Checkpoint. Mol. Biol. Cell 18: 953-964 [Abstract] [Full Text]  
• Schneider, B. L., Zhang, J., Markwardt, J., Tokiwa, G., Volpe, T., Honey, S., Futcher, B. (2004). Growth Rate and Cell Size Modulate the Synthesis of, and Requirement for, G1-Phase Cyclins at Start. Mol. Cell. Biol. 24: 10802-10813 [Abstract] [Full Text]  
• Alberghina, L., Rossi, R. L., Querin, L., Wanke, V., Vanoni, M. (2004). A cell sizer network involving Cln3 and Far1 controls entrance into S phase in the mitotic cycle of budding yeast. J. Cell Biol. 167: 433-443 [Abstract] [Full Text]  
• Newcomb, L. L., Hall, D. D., Heideman, W. (2002). AZF1 Is a Glucose-Dependent Positive Regulator of CLN3 Transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 1607-1614 [Abstract] [Full Text]  
• Cross, F. R., Archambault, V., Miller, M., Klovstad, M. (2002). Testing a Mathematical Model of the Yeast Cell Cycle. Mol. Biol. Cell 13: 52-70 [Abstract] [Full Text]  
• Miller, M. E., Cross, F. R. (2001). Mechanisms Controlling Subcellular Localization of the G1 Cyclins Cln2p and Cln3p in Budding Yeast. Mol. Cell. Biol. 21: 6292-6311 [Abstract] [Full Text]  
• Jacobson, M. D., Munoz, C. X., Knox, K. S., Williams, B. E., Lu, L. L., Cross, F. R., Vallen, E. A. (2001). Mutations in SID2, a Novel Gene in Saccharomyces cerevisiae, Cause Synthetic Lethality With sic1 Deletion and May Cause a Defect During S Phase. Genetics 159: 17-33 [Abstract] [Full Text]  
• MacKay, V. L., Mai, B., Waters, L., Breeden, L. L. (2001). Early Cell Cycle Box-Mediated Transcription of CLN3 and SWI4 Contributes to the Proper Timing of the G1-to-S Transition in Budding Yeast. Mol. Cell. Biol. 21: 4140-4148 [Abstract] [Full Text]  
• Miller, M. E., Cross, F. R. (2000). Distinct Subcellular Localization Patterns Contribute to Functional Specificity of the Cln2 and Cln3 Cyclins of Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 542-555 [Abstract] [Full Text]  
• Hautbergue, G., Goguel, V. (1999). The Yeast C-Type Cyclin Ctk2p Is Phosphorylated and Rapidly Degraded by the Ubiquitin-Proteasome Pathway. Mol. Cell. Biol. 19: 2527-2534 [Abstract] [Full Text]  
• Vallen, E. A., Cross, F. R. (1999). Interaction Between the MEC1-Dependent DNA Synthesis Checkpoint and G1 Cyclin Function in Saccharomyces cerevisiae. Genetics 151: 459-471 [Abstract] [Full Text]  
• Mendenhall, M. D., Hodge, A. E. (1998). Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1191-1243 [Abstract] [Full Text]  
• Gueckel, R., Enenkel, C., Wolf, D. H., Hilt, W. (1998). Mutations in the Yeast Proteasome beta -Type Subunit Pre3 Uncover Position-dependent Effects on Proteasomal Peptidase Activity and in Vivo Function. J. Biol. Chem. 273: 19443-19452 [Abstract] [Full Text]  
• Levine, K., Oehlen, L. J. W. M., Cross, F. R. (1998). Isolation and Characterization of New Alleles of the Cyclin-Dependent Kinase Gene CDC28 with Cyclin-Specific Functional and Biochemical Defects. Mol. Cell. Biol. 18: 290-302 [Abstract] [Full Text]  
• Jeoung, D.-I., Oehlen, L. J. W. M., Cross, F. R. (1998). Cln3-Associated Kinase Activity in Saccharomyces cerevisiae Is Regulated by the Mating Factor Pathway. Mol. Cell. Biol. 18: 433-441 [Abstract] [Full Text]  
• Polymenis, M., Schmidt, E. V. (1997). Coupling of cell division to cell growth by translational control of the G1 cyclin CLN3 in yeast. Genes Dev. 11: 2522-2531 [Abstract] [Full Text]  
• Clurman, B E, Sheaff, R J, Thress, K, Groudine, M, Roberts, J M (1996). Turnover of cyclin E by the ubiquitin-proteasome pathway is regulated by cdk2 binding and cyclin phosphorylation.. Genes Dev. 10: 1979-1990 [Abstract]  
• Koch, C, Schleiffer, A, Ammerer, G, Nasmyth, K (1996). Switching transcription on and off during the yeast cell cycle: Cln/Cdc28 kinases activate bound transcription factor SBF (Swi4/Swi6) at start, whereas Clb/Cdc28 kinases displace it from the promoter in G2.. Genes Dev. 10: 129-141 [Abstract]  
• Stuart, D, Wittenberg, C (1995). CLN3, not positive feedback, determines the timing of CLN2 transcription in cycling cells.. Genes Dev. 9: 2780-2794 [Abstract]  
• Barral, Y, Jentsch, S, Mann, C (1995). G1 cyclin turnover and nutrient uptake are controlled by a common pathway in yeast.. Genes Dev. 9: 399-409 [Abstract]  
• Oehlen, L J, Cross, F R (1994). G1 cyclins CLN1 and CLN2 repress the mating factor response pathway at Start in the yeast cell cycle.. Genes Dev. 8: 1058-1070 [Abstract]  
• Forsburg, S., Nurse, P (1994). Analysis of the Schizosaccharomyces pombe cyclin puc1: evidence for a role in cell cycle exit. J. Cell Sci. 107: 601-613 [Abstract]  
• Hautbergue, G., Goguel, V. (2001). Activation of the Cyclin-dependent Kinase CTDK-I Requires the Heterodimerization of Two Unstable Subunits. J. Biol. Chem. 276: 8005-8013 [Abstract] [Full Text]