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Mol Cell Biol. 1993 July; 13(7): 4445-4457

A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5.

K Kitada, A L Johnson, L H Johnston and A Sugino

Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.

ABSTRACT

We have isolated a multicopy suppressor of the temperature-sensitive growth phenotype of organisms carrying mutations of DBF4, a gene that is required for the initiation of chromosomal DNA replication in Saccharomyces cerevisiae and that interacts with the CDC7 protein kinase. Nucleotide sequence analysis of the suppressor gene, provisionally named MSD2, revealed an open reading frame encoding a protein with a calculated M(r) of 81,024, with amino acid sequence similarity to the catalytic domains of protein kinases. Both genetic linkage and complementation analyses indicated that MSD2 is identical to the cell division cycle gene CDC5. An activity that phosphorylated exogenously added casein was immunoprecipitated by antiserum against a TrpE-Cdc5 fusion protein from lysates of wild-type cells containing CDC5 on a multicopy plasmid but not of cells bearing a small deletion in the predicted protein kinase domain of CDC5 on the plasmid. Deletion of CDC5 was lethal and resulted in a dumbbell-shaped terminal morphology, with the nuclei almost divided but still connected. Consistent with the function at the G2/M boundary, the CDC5 transcript accumulated periodically during the cell cycle, peaking at the G2/M boundary. CDC5 on a multicopy plasmid also suppresses temperature-sensitive cdc15, cdc20, and dbf2 mutations which affect mitosis during the cell cycle.

Mol Cell Biol. 1993 July; 13(7): 4445-4457




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  • Glover, D. M., Hagan, I. M., Tavares, A. A.M. (1998). Polo-like kinases: a team that plays throughout mitosis. Genes Dev. 12: 3777-3787 [Full Text]  
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  • Carmena, M., Riparbelli, M. G., Minestrini, G., Tavares, A. M., Adams, R., Callaini, G., Glover, D. M. (1998). Drosophila Polo Kinase Is Required for Cytokinesis. JCB 143: 659-671 [Abstract] [Full Text]  
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  • Hamada, K., Terashima, H., Arisawa, M., Kitada, K. (1998). Amino Acid Sequence Requirement for Efficient Incorporation of Glycosylphosphatidylinositol-associated Proteins into the Cell Wall of Saccharomyces cerevisiae. J. Biol. Chem. 273: 26946-26953 [Abstract] [Full Text]  
  • Jaspersen, S. L., Charles, J. F., Tinker-Kulberg, R. L., Morgan, D. O. (1998). A Late Mitotic Regulatory Network Controlling Cyclin Destruction in Saccharomyces cerevisiae. Mol. Biol. Cell 9: 2803-2817 [Abstract] [Full Text]  
  • Lee, K. S., Grenfell, T. Z., Yarm, F. R., Erikson, R. L. (1998). Mutation of the polo-box disrupts localization and mitotic functions of the mammalian polo kinase Plk. Proc. Natl. Acad. Sci. USA 95: 9301-9306 [Abstract] [Full Text]  
  • Komarnitsky, S. I., Chiang, Y.-C., Luca, F. C., Chen, J., Toyn, J. H., Winey, M., Johnston, L. H., Denis, C. L. (1998). DBF2 Protein Kinase Binds to and Acts through the Cell Cycle-Regulated MOB1 Protein. Mol. Cell. Biol. 18: 2100-2107 [Abstract] [Full Text]  
  • Aerne, B. L., Johnson, A. L., Toyn, J. H., Johnston, L. H. (1998). Swi5 Controls a Novel Wave of Cyclin Synthesis in Late Mitosis. Mol. Biol. Cell 9: 945-956 [Abstract] [Full Text]  
  • Bousset, K., Diffley, J. F.X. (1998). The Cdc7 protein kinase is required for origin firing during S phase. Genes Dev. 12: 480-490 [Abstract] [Full Text]  
  • Lippincott, J., Li, R. (1998). Sequential Assembly of Myosin II, an IQGAP-like Protein, and Filamentous Actin to a Ring Structure Involved in Budding Yeast Cytokinesis. JCB 140: 355-366 [Abstract] [Full Text]  
  • Logarinho, E, Sunkel, C. (1998). The Drosophila POLO kinase localises to multiple compartments of the mitotic apparatus and is required for the phosphorylation of MPM2 reactive epitopes. J. Cell Sci. 111: 2897-2909 [Abstract]  
  • Abrieu, A, Brassac, T, Galas, S, Fisher, D, Labbe, J., Doree, M (1998). The Polo-like kinase Plx1 is a component of the MPF amplification loop at the G2/M-phase transition of the cell cycle in Xenopus eggs. J. Cell Sci. 111: 1751-1757 [Abstract]  
  • Luca, F. C., Winey, M. (1998). MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy. Mol. Biol. Cell 9: 29-46 [Abstract] [Full Text]  
  • Lei, M., Kawasaki, Y., Young, M. R., Kihara, M., Sugino, A., Tye, B. K. (1997). Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis. Genes Dev. 11: 3365-3374 [Abstract] [Full Text]  
  • Gould, K. L., Simanis, V. (1997). The control of septum formation in fission yeast. Genes Dev. 11: 2939-2951 [Full Text]  
  • Ouyang, B., Pan, H., Lu, L., Li, J., Stambrook, P., Li, B., Dai, W. (1997). Human Prk Is a Conserved Protein Serine/Threonine Kinase Involved in Regulating M Phase Functions. J. Biol. Chem. 272: 28646-28651 [Abstract] [Full Text]  
  • Schmidt, S, Sohrmann, M, Hofmann, K, Woollard, A, Simanis, V (1997). The Spg1p GTPase is an essential, dosage-dependent inducer of septum formation in Schizosaccharomyces pombe.. Genes Dev. 11: 1519-1534 [Abstract]  
  • Kimura, M., Kotani, S., Hattori, T., Sumi, N., Yoshioka, T., Todokoro, K., Okano, Y. (1997). Cell Cycle-dependent Expression and Spindle Pole Localization of a Novel Human Protein Kinase, Aik, Related to Aurora of Drosophila and Yeast Ipl1. J. Biol. Chem. 272: 13766-13771 [Abstract] [Full Text]  
  • Stillman, B. (1996). Cell Cycle Control of DNA Replication. Science 274: 1659-1663 [Abstract] [Full Text]  
  • Kumagai, A., Dunphy, W. G. (1996). Purification and Molecular Cloning of Plx1, a Cdc25-Regulatory Kinase from Xenopus Egg Extracts. Science 273: 1377-1380 [Abstract]  
  • Li, B., Ouyang, B., Pan, H., Reissmann, PeterT., Slamon, DennisJ., Arceci, R., Lu, L., Dai, W. (1996). prk, a Cytokine-inducible Human Protein Serine/Threonine Kinase Whose Expression Appears to be Down-regulated in Lung Carcinomas. J. Biol. Chem. 271: 19402-19408 [Abstract] [Full Text]  
  • Morishita, T., Mitsuzawa, H., Nakafuku, M., Nakamura, S., Hattori, S., Anraku, Y. (1995). Requirement of Saccharomyces cerevisiae Ras for Completion of Mitosis. Science 270: 1213-1215 [Abstract]  
  • Hamanaka, R., Smith, M. R., O'Connor, P. M., Maloid, S., Mihalic, K., Spivak, J. L., Longo, D. L., Ferris, D. K. (1995). Polo-like Kinase Is a Cell Cycle-regulated Kinase Activated during Mitosis. J. Biol. Chem. 270: 21086-21091 [Abstract] [Full Text]  
  • Ohkura, H, Hagan, I M, Glover, D M (1995). The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells.. Genes Dev. 9: 1059-1073 [Abstract]  
  • Donohue, P. J., Alberts, G. F., Guo, Y., Winkles, J. A. (1995). Identification by Targeted Differential Display of an Immediate Early Gene Encoding a Putative Serine/Threonine Kinase. J. Biol. Chem. 270: 10351-10357 [Abstract] [Full Text]  
  • Donovan, J D, Toyn, J H, Johnson, A L, Johnston, L H (1994). P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae.. Genes Dev. 8: 1640-1653 [Abstract]  
  • Golsteyn, R., Schultz, S., Bartek, J, Ziemiecki, A, Ried, T, Nigg, E. (1994). Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5. J. Cell Sci. 107: 1509-1517 [Abstract]  
  • Xie, S., Wang, Q., Wu, H., Cogswell, J., Lu, L., Jhanwar-Uniyal, M., Dai, W. (2001). Reactive Oxygen Species-induced Phosphorylation of p53 on Serine 20 Is Mediated in Part by Polo-like Kinase-3. J. Biol. Chem. 276: 36194-36199 [Abstract] [Full Text]  
  • Mah, A. S., Jang, J., Deshaies, R. J. (2001). Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc. Natl. Acad. Sci. USA 98: 7325-7330 [Abstract] [Full Text]  


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