This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xiao, Z
Right arrow Articles by Fitzgerald-Hayes, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xiao, Z
Right arrow Articles by Fitzgerald-Hayes, M

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 August; 13(8): 4691-4702

CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae.

Z Xiao, J T McGrew, A J Schroeder and M Fitzgerald-Hayes

Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003.

ABSTRACT

By monitoring the mitotic transmission of a marked chromosome bearing a defective centromere, we have identified conditional alleles of two genes involved in chromosome segregation (cse). Mutations in CSE1 and CSE2 have a greater effect on the segregation of chromosomes carrying mutant centromeres than on the segregation of chromosomes with wild-type centromeres. In addition, the cse mutations cause predominantly nondisjunction rather than loss events but do not cause a detectable increase in mitotic recombination. At the restrictive temperature, cse1 and cse2 mutants accumulate large-budded cells, with a significant fraction exhibiting aberrant binucleate morphologies. We cloned the CSE1 and CSE2 genes by complementation of the cold-sensitive phenotypes. Physical and genetic mapping data indicate that CSE1 is linked to HAP2 on the left arm of chromosome VII and CSE2 is adjacent to PRP2 on chromosome XIV. CSE1 is essential and encodes a novel 109-kDa protein. CSE2 encodes a 17-kDa protein with a putative basic-region leucine zipper motif. Disruption of CSE2 causes chromosome missegregation, conditional lethality, and slow growth at the permissive temperature.

Mol Cell Biol. 1993 August; 13(8): 4691-4702




This article has been cited by other articles:

  • White, M. A., Riles, L., Cohen, B. A. (2009). A Systematic Screen for Transcriptional Regulators of the Yeast Cell Cycle. Genetics 181: 435-446 [Abstract] [Full Text]  
  • Takahashi, H., Kasahara, K., Kokubo, T. (2009). Saccharomyces cerevisiae Med9 comprises two functionally distinct domains that play different roles in transcriptional regulation. GENES CELLS 14: 53-67 [Abstract] [Full Text]  
  • Wong, J., Nakajima, Y., Westermann, S., Shang, C., Kang, J.-s., Goodner, C., Houshmand, P., Fields, S., Chan, C. S.M., Drubin, D., Barnes, G., Hazbun, T. (2007). A Protein Interaction Map of the Mitotic Spindle. Mol. Biol. Cell 18: 3800-3809 [Abstract] [Full Text]  
  • Yu, L., Castillo, L. P., Mnaimneh, S., Hughes, T. R., Brown, G. W. (2006). A Survey of Essential Gene Function in the Yeast Cell Division Cycle. Mol. Biol. Cell 17: 4736-4747 [Abstract] [Full Text]  
  • Butterfield-Gerson, K. L., Scheifele, L. Z., Ryan, E. P., Hopper, A. K., Parent, L. J. (2006). Importin-{beta} Family Members Mediate Alpharetrovirus Gag Nuclear Entry via Interactions with Matrix and Nucleocapsid. J. Virol. 80: 1798-1806 [Abstract] [Full Text]  
  • Strahl, T., Hama, H., DeWald, D. B., Thorner, J. (2005). Yeast phosphatidylinositol 4-kinase, Pik1, has essential roles at the Golgi and in the nucleus. JCB 171: 967-979 [Abstract] [Full Text]  
  • Wermuth, P. J., Buchberg, A. M. (2005). Meis1-mediated apoptosis is caspase dependent and can be suppressed by coexpression of HoxA9 in murine and human cell lines. Blood 105: 1222-1230 [Abstract] [Full Text]  
  • Freedman, N. D., Yamamoto, K. R. (2004). Importin 7 and Importin {alpha}/Importin {beta} Are Nuclear Import Receptors for the Glucocorticoid Receptor. Mol. Biol. Cell 15: 2276-2286 [Abstract] [Full Text]  
  • Harreman, M. T., Cohen, P. E., Hodel, M. R., Truscott, G. J., Corbett, A. H., Hodel, A. E. (2003). Characterization of the Auto-inhibitory Sequence within the N-terminal Domain of Importin {alpha}. J. Biol. Chem. 278: 21361-21369 [Abstract] [Full Text]  
  • Harreman, M. T., Hodel, M. R., Fanara, P., Hodel, A. E., Corbett, A. H. (2003). The Auto-inhibitory Function of Importin alpha Is Essential in Vivo. J. Biol. Chem. 278: 5854-5863 [Abstract] [Full Text]  
  • Asakawa, K., Toh-e, A. (2002). A Defect of Kap104 Alleviates the Requirement of Mitotic Exit Network Gene Functions in Saccharomyces cerevisiae. Genetics 162: 1545-1556 [Abstract] [Full Text]  
  • Feng, W., Hopper, A. K. (2002). A Los1p-independent pathway for nuclear export of intronless tRNAs in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 99: 5412-5417 [Abstract] [Full Text]  
  • Bera, T. K., Bera, J., Brinkmann, U., Tessarollo, L., Pastan, I. (2001). Cse1l Is Essential for Early Embryonic Growth and Development. Mol. Cell. Biol. 21: 7020-7024 [Abstract] [Full Text]  
  • Polizotto, R. S., Cyert, M. S. (2001). Calcineurin-dependent nuclear import of the transcription factor Crz1p requires Nmd5p. JCB 154: 951-960 [Abstract] [Full Text]  
  • Zenklusen, D., Vinciguerra, P., Strahm, Y., Stutz, F. (2001). The Yeast hnRNP-Like Proteins Yra1p and Yra2p Participate in mRNA Export through Interaction with Mex67p. Mol. Cell. Biol. 21: 4219-4232 [Abstract] [Full Text]  
  • Künzler, M., Trueheart, J., Sette, C., Hurt, E., Thorner, J. (2001). Mutations in the YRB1 Gene Encoding Yeast Ran-Binding-Protein-1 That Impair Nucleocytoplasmic Transport and Suppress Yeast Mating Defects. Genetics 157: 1089-1105 [Abstract] [Full Text]  
  • Stage-Zimmermann, T., Schmidt, U., Silver, P. A. (2000). Factors Affecting Nuclear Export of the 60S Ribosomal Subunit In Vivo. Mol. Biol. Cell 11: 3777-3789 [Abstract] [Full Text]  
  • Peng, G., Hopper, J. E. (2000). Evidence for Gal3p's Cytoplasmic Location and Gal80p's Dual Cytoplasmic-Nuclear Location Implicates New Mechanisms for Controlling Gal4p Activity in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 5140-5148 [Abstract] [Full Text]  
  • Künzler, M., Gerstberger, T., Stutz, F., Bischoff, F. R., Hurt, E. (2000). Yeast Ran-Binding Protein 1 (Yrb1) Shuttles between the Nucleus and Cytoplasm and Is Exported from the Nucleus via a CRM1 (XPO1)-Dependent Pathway. Mol. Cell. Biol. 20: 4295-4308 [Abstract] [Full Text]  
  • Booth, J. W., Belanger, K. D., Sannella, M. I., Davis, L. I. (1999). The Yeast Nucleoporin Nup2p Is Involved in Nuclear Export of Importin alpha /Srp1p. J. Biol. Chem. 274: 32360-32367 [Abstract] [Full Text]  
  • Moy, T. I., Silver, P. A. (1999). Nuclear export of the small ribosomal subunit requires the Ran-GTPase cycle and certain nucleoporins. Genes Dev. 13: 2118-2133 [Abstract] [Full Text]  
  • Pemberton, L. F., Rosenblum, J. S., Blobel, G. (1999). Nuclear Import of the TATA-binding Protein: Mediation by the Karyopherin Kap114p and a Possible Mechanism for Intranuclear Targeting. JCB 145: 1407-1417 [Abstract] [Full Text]  
  • Liu, Y., Guo, W., Tartakoff, P. Y., Tartakoff, A. M. (1999). A Crm1p-independent nuclear export path for the mRNA-associated protein, Npl3p/Mtr13p. Proc. Natl. Acad. Sci. USA 96: 6739-6744 [Abstract] [Full Text]  
  • Han, S. J., Lee, Y. C., Gim, B. S., Ryu, G.-H., Park, S. J., Lane, W. S., Kim, Y.-J. (1999). Activator-Specific Requirement of Yeast Mediator Proteins for RNA Polymerase II Transcriptional Activation. Mol. Cell. Biol. 19: 979-988 [Abstract] [Full Text]  
  • Hood, J. K., Silver, P. A. (1998). Cse1p Is Required for Export of Srp1p/Importin-alpha from the Nucleus in Saccharomyces cerevisiae. J. Biol. Chem. 273: 35142-35146 [Abstract] [Full Text]  
  • Gustafsson, C. M., Myers, L. C., Beve, J., Spahr, H., Lui, M., Erdjument-Bromage, H., Tempst, P., Kornberg, R. D. (1998). Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae. J. Biol. Chem. 273: 30851-30854 [Abstract] [Full Text]  
  • Solsbacher, J., Maurer, P., Bischoff, F. R., Schlenstedt, G. (1998). Cse1p Is Involved in Export of Yeast Importin alpha  from the Nucleus. Mol. Cell. Biol. 18: 6805-6815 [Abstract] [Full Text]  
  • Kaffman, A., Rank, N. M., O'Shea, E. K. (1998). Phosphorylation regulates association of the transcription factor Pho4 with its import receptor Pse1/Kap121. Genes Dev. 12: 2673-2683 [Abstract] [Full Text]  
  • Taura, T., Krebber, H., Silver, P. A. (1998). A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export. Proc. Natl. Acad. Sci. USA 95: 7427-7432 [Abstract] [Full Text]  
  • Hwang, L. H., Murray, A. W. (1997). A Novel Yeast Screen for Mitotic Arrest Mutants Identifies DOC1, a New Gene Involved in Cyclin Proteolysis. Mol. Biol. Cell 8: 1877-1887 [Abstract] [Full Text]  
  • Seedorf, M., Silver, P. A. (1997). Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc. Natl. Acad. Sci. USA 94: 8590-8595 [Abstract] [Full Text]  
  • Gorlich, D., Dabrowski, M., Bischoff, F. R., Kutay, U., Bork, P., Hartmann, E., Prehn, S., Izaurralde, E. (1997). A Novel Class of RanGTP Binding Proteins. JCB 138: 65-80 [Abstract] [Full Text]  
  • Brinkmann, U, Gallo, M, Polymeropoulos, M H, Pastan, I (1996). The human CAS (cellular apoptosis susceptibility) gene mapping on chromosome 20q13 is amplified in BT474 breast cancer cells and part of aberrant chromosomes in breast and colon cancer cell lines.. Genome Res 6: 187-194 [Abstract]  
  • Stoler, S, Keith, K C, Curnick, K E, Fitzgerald-Hayes, M (1995). A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis.. Genes Dev. 9: 573-586 [Abstract]  
  • Baumer, M., Kunzler, M., Steigemann, P., Braus, G. H., Irniger, S. (2000). Yeast Ran-binding Protein Yrb1p Is Required for Efficient Proteolysis of Cell Cycle Regulatory Proteins Pds1p and Sic1p. J. Biol. Chem. 275: 38929-38937 [Abstract] [Full Text]  
  • Carvalho, J., Bertram, P. G., Wente, S. R., Zheng, X. F. S. (2001). Phosphorylation Regulates the Interaction between Gln3p and the Nuclear Import Factor Srp1p. J. Biol. Chem. 276: 25359-25365 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»