This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hsu, C L Articles by Stevens, A Search for Related Content PubMed PubMed Citation Articles by Hsu, C L Articles by Stevens, A

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 August; 13(8): 4826-4835

Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure.

Biology Division, Oak Ridge National Laboratory, Tennessee 37831-8077.

ABSTRACT

Analysis of the slowed turnover rates of several specific mRNA species and the higher cellular levels of some of these mRNAs in Saccharomyces cerevisiae lacking 5'-->3' exoribonuclease 1 (xrn1 cells) has led to the finding that these yeast contain higher amounts of essentially full-length mRNAs that do not bind to oligo(dT)-cellulose. On the other hand, the length of mRNA poly(A) chains found after pulse-labeling of cells lacking the exoribonuclease, the cellular rate of synthesis of oligo(dT)-bound mRNA, and the initial rate of its deadenylation appeared quite similar to the same measurements in wild-type yeast cells. Examination of the 5' cap structure status of the poly(A)-deficient mRNAs by comparative analysis of the m7G content of poly(A)- and poly(A)+ RNA fractions of wild-type and xrn1 cells suggested that the xrn1 poly(A)- mRNA fraction is low in cap structure content. Further analysis of the 5' termini by measurements of the rate of 5'-->3' exoribonuclease 1 hydrolysis of specific full-length mRNA species showed that approximately 50% of the xrn1 poly(A)-deficient mRNA species lack the cap structure. Primer extension analysis of the 5' terminus of ribosomal protein 51A (RP51A) mRNA showed that about 30% of the poly(A)-deficient molecules of the xrn1 cells are slightly shorter at the 5' end. The finding of some accumulation of poly(A)-deficient mRNA species partially lacking the cap structure together with the reduction of the rate of mRNA turnover in cells lacking the enzyme suggest a possible role for 5'-->3' exoribonuclease 1 in the mRNA turnover process.

This article has been cited by other articles:

• Chowdhury, A., Tharun, S. (2008). lsm1 mutations impairing the ability of the Lsm1p-7p-Pat1p complex to preferentially bind to oligoadenylated RNA affect mRNA decay in vivo. RNA 14: 2149-2158 [Abstract] [Full Text]  
• Rendl, L. M., Bieman, M. A., Smibert, C. A. (2008). S. cerevisiae Vts1p induces deadenylation-dependent transcript degradation and interacts with the Ccr4p-Pop2p-Not deadenylase complex. RNA 14: 1328-1336 [Abstract] [Full Text]  
• Liu, S.-W., Rajagopal, V., Patel, S. S., Kiledjian, M. (2008). Mechanistic and Kinetic Analysis of the DcpS Scavenger Decapping Enzyme. J. Biol. Chem. 283: 16427-16436 [Abstract] [Full Text]  
• Tornaletti, S., Park-Snyder, S., Hanawalt, P. C. (2008). G4-forming Sequences in the Non-transcribed DNA Strand Pose Blocks to T7 RNA Polymerase and Mammalian RNA Polymerase II. J. Biol. Chem. 283: 12756-12762 [Abstract] [Full Text]  
• Pilkington, G. R., Parker, R. (2008). Pat1 Contains Distinct Functional Domains That Promote P-Body Assembly and Activation of Decapping. Mol. Cell. Biol. 28: 1298-1312 [Abstract] [Full Text]  
• Li, Y., Song, M.-G., Kiledjian, M. (2008). Transcript-Specific Decapping and Regulated Stability by the Human Dcp2 Decapping Protein. Mol. Cell. Biol. 28: 939-948 [Abstract] [Full Text]  
• Gunawardana, D., Cheng, H.-C., Gayler, K. R. (2008). Identification of functional domains in Arabidopsis thaliana mRNA decapping enzyme (AtDcp2). Nucleic Acids Res 36: 203-216 [Abstract] [Full Text]  
• Song, M.-G., Kiledjian, M. (2007). 3' Terminal oligo U-tract-mediated stimulation of decapping. RNA 13: 2356-2365 [Abstract] [Full Text]  
• Grudzien-Nogalska, E., Jemielity, J., Kowalska, J., Darzynkiewicz, E., Rhoads, R. E. (2007). Phosphorothioate cap analogs stabilize mRNA and increase translational efficiency in mammalian cells. RNA 13: 1745-1755 [Abstract] [Full Text]  
• Wilson, M. A., Meaux, S., van Hoof, A. (2007). A Genomic Screen in Yeast Reveals Novel Aspects of Nonstop mRNA Metabolism. Genetics 177: 773-784 [Abstract] [Full Text]  
• Bonisch, C., Temme, C., Moritz, B., Wahle, E. (2007). Degradation of hsp70 and Other mRNAs in Drosophila via the 5' 3' Pathway and Its Regulation by Heat Shock. J. Biol. Chem. 282: 21818-21828 [Abstract] [Full Text]  
• Chowdhury, A., Mukhopadhyay, J., Tharun, S. (2007). The decapping activator Lsm1p-7p-Pat1p complex has the intrinsic ability to distinguish between oligoadenylated and polyadenylated RNAs. RNA 13: 998-1016 [Abstract] [Full Text]  
• Parrish, S., Resch, W., Moss, B. (2007). Vaccinia virus D10 protein has mRNA decapping activity, providing a mechanism for control of host and viral gene expression. Proc. Natl. Acad. Sci. USA 104: 2139-2144 [Abstract] [Full Text]  
• Li, C.-H., Irmer, H., Gudjonsdottir-Planck, D., Freese, S., Salm, H., Haile, S., Estevez, A. M., Clayton, C. (2006). Roles of a Trypanosoma brucei 5'->3' exoribonuclease homolog in mRNA degradation. RNA 12: 2171-2186 [Abstract] [Full Text]  
• Segal, S. P., Dunckley, T., Parker, R. (2006). Sbp1p Affects Translational Repression and Decapping in Saccharomyces cerevisiae.. Mol. Cell. Biol. 26: 5120-5130 [Abstract] [Full Text]  
• Grudzien, E., Kalek, M., Jemielity, J., Darzynkiewicz, E., Rhoads, R. E. (2006). Differential Inhibition of mRNA Degradation Pathways by Novel Cap Analogs. J. Biol. Chem. 281: 1857-1867 [Abstract] [Full Text]  
• Lall, S., Piano, F., Davis, R. E. (2005). Caenorhabditis elegans Decapping Proteins: Localization and Functional Analysis of Dcp1, Dcp2, and DcpS during Embryogenesis. Mol. Biol. Cell 16: 5880-5890 [Abstract] [Full Text]  
• Liu, H., Kiledjian, M. (2005). Scavenger Decapping Activity Facilitates 5' to 3' mRNA Decay. Mol. Cell. Biol. 25: 9764-9772 [Abstract] [Full Text]  
• WANG, L., LEWIS, M. S., JOHNSON, A. W. (2005). Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p. RNA 11: 1291-1302 [Abstract] [Full Text]  
• Tharun, S., Muhlrad, D., Chowdhury, A., Parker, R. (2005). Mutations in the Saccharomyces cerevisiae LSM1 Gene That Affect mRNA Decapping and 3' End Protection. Genetics 170: 33-46 [Abstract] [Full Text]  
• TEIXEIRA, D., SHETH, U., VALENCIA-SANCHEZ, M. A., BRENGUES, M., PARKER, R. (2005). Processing bodies require RNA for assembly and contain nontranslating mRNAs. RNA 11: 371-382 [Abstract] [Full Text]  
• Ross, P. L., Huang, Y. N., Marchese, J. N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Dey, S., Daniels, S., Purkayastha, S., Juhasz, P., Martin, S., Bartlet-Jones, M., He, F., Jacobson, A., Pappin, D. J. (2004). Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents. Mol. Cell. Proteomics 3: 1154-1169 [Abstract] [Full Text]  
• Kufel, J., Bousquet-Antonelli, C., Beggs, J. D., Tollervey, D. (2004). Nuclear Pre-mRNA Decapping and 5' Degradation in Yeast Require the Lsm2-8p Complex. Mol. Cell. Biol. 24: 9646-9657 [Abstract] [Full Text]  
• JACKSON, J. S. JR., HOUSHMANDI, S. S., LOPEZ LEBAN, F., OLIVAS, W. M. (2004). Recruitment of the Puf3 protein to its mRNA target for regulation of mRNA decay in yeast. RNA 10: 1625-1636 [Abstract] [Full Text]  
• Duquette, M. L., Handa, P., Vincent, J. A., Taylor, A. F., Maizels, N. (2004). Intracellular transcription of G-rich DNAs induces formation of G-loops, novel structures containing G4 DNA. Genes Dev. 18: 1618-1629 [Abstract] [Full Text]  
• Grigull, J., Mnaimneh, S., Pootoolal, J., Robinson, M. D., Hughes, T. R. (2004). Genome-Wide Analysis of mRNA Stability Using Transcription Inhibitors and Microarrays Reveals Posttranscriptional Control of Ribosome Biogenesis Factors. Mol. Cell. Biol. 24: 5534-5547 [Abstract] [Full Text]  
• Holmes, L. E. A., Campbell, S. G., De Long, S. K., Sachs, A. B., Ashe, M. P. (2004). Loss of Translational Control in Yeast Compromised for the Major mRNA Decay Pathway. Mol. Cell. Biol. 24: 2998-3010 [Abstract] [Full Text]  
• BENARD, L. (2004). Inhibition of 5' to 3' mRNA degradation under stress conditions in Saccharomyces cerevisiae: from GCN4 to MET16. RNA 10: 458-468 [Abstract] [Full Text]  
• Kshirsagar, M., Parker, R. (2004). Identification of Edc3p as an Enhancer of mRNA Decapping in Saccharomyces cerevisiae. Genetics 166: 729-739 [Abstract] [Full Text]  
• van Dijk, E., Le Hir, H., Seraphin, B. (2003). DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway. Proc. Natl. Acad. Sci. USA 100: 12081-12086 [Abstract] [Full Text]  
• PICCIRILLO, C., KHANNA, R., KILEDJIAN, M. (2003). Functional characterization of the mammalian mRNA decapping enzyme hDcp2. RNA 9: 1138-1147 [Abstract] [Full Text]  
• Das, B., Butler, J. S., Sherman, F. (2003). Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae. Mol. Cell. Biol. 23: 5502-5515 [Abstract] [Full Text]  
• Kebaara, B., Nazarenus, T., Taylor, R., Forch, A., Atkin, A. L. (2003). The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides. Nucleic Acids Res 31: 3157-3165 [Abstract] [Full Text]  
• Sheth, U., Parker, R. (2003). Decapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies. Science 300: 805-808 [Abstract] [Full Text]  
• Wu, X., Guarino, L. A. (2003). Autographa californica Nucleopolyhedrovirus orf69 Encodes an RNA Cap (Nucleoside-2'-O)-Methyltransferase. J. Virol. 77: 3430-3440 [Abstract] [Full Text]  
• Esteban, R., Fujimura, T. (2003). Launching the yeast 23S RNA Narnavirus shows 5' and 3' cis-acting signals for replication. Proc. Natl. Acad. Sci. USA 100: 2568-2573 [Abstract] [Full Text]  
• STEIGER, M., CARR-SCHMID, A., SCHWARTZ, D. C., KILEDJIAN, M., PARKER, R. (2003). Analysis of recombinant yeast decapping enzyme. RNA 9: 231-238 [Abstract] [Full Text]  
• SCHWARTZ, D., DECKER, C. J., PARKER, R. (2003). The enhancer of decapping proteins, Edc1p and Edc2p, bind RNA and stimulate the activity of the decapping enzyme. RNA 9: 239-251 [Abstract] [Full Text]  
• Maderazo, A. B., Belk, J. P., He, F., Jacobson, A. (2003). Nonsense-Containing mRNAs That Accumulate in the Absence of a Functional Nonsense-Mediated mRNA Decay Pathway Are Destabilized Rapidly upon Its Restitution. Mol. Cell. Biol. 23: 842-851 [Abstract] [Full Text]  
• Kufel, J., Allmang, C., Petfalski, E., Beggs, J., Tollervey, D. (2003). Lsm Proteins Are Required for Normal Processing and Stability of Ribosomal RNAs. J. Biol. Chem. 278: 2147-2156 [Abstract] [Full Text]  
• Lykke-Andersen, J. (2002). Identification of a Human Decapping Complex Associated with hUpf Proteins in Nonsense-Mediated Decay. Mol. Cell. Biol. 22: 8114-8121 [Abstract] [Full Text]  
• Brew, C. T., Huffaker, T. C. (2002). The Yeast Ubiquitin Protease, Ubp3p, Promotes Protein Stability. Genetics 162: 1079-1089 [Abstract] [Full Text]  
• Oliveira, C. C., Gonzales, F. A., Zanchin, N. I. T. (2002). Temperature-sensitive mutants of the exosome subunit Rrp43p show a deficiency in mRNA degradation and no longer interact with the exosome. Nucleic Acids Res 30: 4186-4198 [Abstract] [Full Text]  
• Decker, C. J., Parker, R. (2002). mRNA decay enzymes: Decappers conserved between yeast and mammals. Proc. Natl. Acad. Sci. USA 99: 12512-12514 [Full Text]  
• Akgul, B., Tu, C.-P. D. (2002). Evidence for a Stabilizer Element in the Untranslated Regions of Drosophila Glutathione S-Transferase D1 mRNA. J. Biol. Chem. 277: 34700-34707 [Abstract] [Full Text]  
• Keogh, M.-C., Cho, E.-J., Podolny, V., Buratowski, S. (2002). Kin28 Is Found within TFIIH and a Kin28-Ccl1-Tfb3 Trimer Complex with Differential Sensitivities to T-Loop Phosphorylation. Mol. Cell. Biol. 22: 1288-1297 [Abstract] [Full Text]  
• Bond, A. T., Mangus, D. A., He, F., Jacobson, A. (2001). Absence of Dbp2p Alters Both Nonsense-Mediated mRNA Decay and rRNA Processing. Mol. Cell. Biol. 21: 7366-7379 [Abstract] [Full Text]  
• Albig, A. R., Decker, C. J. (2001). The Target of Rapamycin Signaling Pathway Regulates mRNA Turnover in the Yeast Saccharomyces cerevisiae. Mol. Biol. Cell 12: 3428-3438 [Abstract] [Full Text]  
• Searfoss, A., Dever, T. E., Wickner, R. (2001). Linking the 3' Poly(A) Tail to the Subunit Joining Step of Translation Initiation: Relations of Pab1p, Eukaryotic Translation Initiation Factor 5B (Fun12p), and Ski2p-Slh1p. Mol. Cell. Biol. 21: 4900-4908 [Abstract] [Full Text]  
• Daugeron, M.-C., Mauxion, F., Seraphin, B. (2001). The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation. Nucleic Acids Res 29: 2448-2455 [Abstract] [Full Text]  
• Shobuike, T., Tatebayashi, K., Tani, T., Sugano, S., Ikeda, H. (2001). The dhp1+ gene, encoding a putative nuclear 5'{->}3' exoribonuclease, is required for proper chromosome segregation in fission yeast. Nucleic Acids Res 29: 1326-1333 [Abstract] [Full Text]  
• He, F., Jacobson, A. (2001). Upf1p, Nmd2p, and Upf3p Regulate the Decapping and Exonucleolytic Degradation of both Nonsense-Containing mRNAs and Wild-Type mRNAs. Mol. Cell. Biol. 21: 1515-1530 [Abstract] [Full Text]  
• Dunckley, T., Tucker, M., Parker, R. (2001). Two Related Proteins, Edc1p and Edc2p, Stimulate mRNA Decapping in Saccharomyces cerevisiae. Genetics 157: 27-37 [Abstract] [Full Text]  
• Kastenmayer, J. P., Green, P. J. (2000). Novel features of the XRN-family in Arabidopsis: Evidence that AtXRN4, one of several orthologs of nuclear Xrn2p/Rat1p, functions in the cytoplasm. Proc. Natl. Acad. Sci. USA 97: 13985-13990 [Abstract] [Full Text]  
• Schwartz, D. C., Parker, R. (2000). mRNA Decapping in Yeast Requires Dissociation of the Cap Binding Protein, Eukaryotic Translation Initiation Factor 4E. Mol. Cell. Biol. 20: 7933-7942 [Abstract] [Full Text]  
• Ahola, T., den Boon, J. A., Ahlquist, P. (2000). Helicase and Capping Enzyme Active Site Mutations in Brome Mosaic Virus Protein 1a Cause Defects in Template Recruitment, Negative-Strand RNA Synthesis, and Viral RNA Capping. J. Virol. 74: 8803-8811 [Abstract] [Full Text]  
• Searfoss, A. M., Wickner, R. B. (2000). 3' poly(A) is dispensable for translation. Proc. Natl. Acad. Sci. USA 97: 9133-9137 [Abstract] [Full Text]  
• Maderazo, A. B., He, F., Mangus, D. A., Jacobson, A. (2000). Upf1p Control of Nonsense mRNA Translation Is Regulated by Nmd2p and Upf3p. Mol. Cell. Biol. 20: 4591-4603 [Abstract] [Full Text]  
• Xue, Y., Bai, X., Lee, I., Kallstrom, G., Ho, J., Brown, J., Stevens, A., Johnson, A. W. (2000). Saccharomyces cerevisiae RAI1 (YGL246c) Is Homologous to Human DOM3Z and Encodes a Protein That Binds the Nuclear Exoribonuclease Rat1p. Mol. Cell. Biol. 20: 4006-4015 [Abstract] [Full Text]  
• Brown, J. T., Yang, X., Johnson, A. W. (2000). Inhibition of mRNA Turnover in Yeast by an xrn1 Mutation Enhances the Requirement for eIF4E Binding to eIF4G and for Proper Capping of Transcripts by Ceg1p. Genetics 155: 31-42 [Abstract] [Full Text]  
• Burkard, K. T. D., Butler, J. S. (2000). A Nuclear 3'-5' Exonuclease Involved in mRNA Degradation Interacts with Poly(A) Polymerase and the hnRNA Protein Npl3p. Mol. Cell. Biol. 20: 604-616 [Abstract] [Full Text]  
• Zhang, S., Williams, C. J., Hagan, K., Peltz, S. W. (1999). Mutations in VPS16 and MRT1 Stabilize mRNAs by Activating an Inhibitor of the Decapping Enzyme. Mol. Cell. Biol. 19: 7568-7576 [Abstract] [Full Text]  
• Muhlrad, D., Parker, R. (1999). Recognition of Yeast mRNAs as "Nonsense Containing" Leads to Both Inhibition of mRNA Translation and mRNA Degradation: Implications for the Control of mRNA Decapping. Mol. Biol. Cell 10: 3971-3978 [Abstract] [Full Text]  
• Solinger, J. A., Pascolini, D., Heyer, W.-D. (1999). Active-Site Mutations in the Xrn1p Exoribonuclease of Saccharomyces cerevisiae Reveal a Specific Role in Meiosis. Mol. Cell. Biol. 19: 5930-5942 [Abstract] [Full Text]  
• Page, M. F., Carr, B., Anders, K. R., Grimson, A., Anderson, P. (1999). SMG-2 Is a Phosphorylated Protein Required for mRNA Surveillance in Caenorhabditis elegans and Related to Upf1p of Yeast. Mol. Cell. Biol. 19: 5943-5951 [Abstract] [Full Text]  
• Zuk, D., Belk, J. P., Jacobson, A. (1999). Temperature-Sensitive Mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 Genes Result in Defects in mRNA Turnover. Genetics 153: 35-47 [Abstract] [Full Text]  
• Moy, T. I., Silver, P. A. (1999). Nuclear export of the small ribosomal subunit requires the Ran-GTPase cycle and certain nucleoporins. Genes Dev. 13: 2118-2133 [Abstract] [Full Text]  
• Schwartz, D. C., Parker, R. (1999). Mutations in Translation Initiation Factors Lead to Increased Rates of Deadenylation and Decapping of mRNAs in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 5247-5256 [Abstract] [Full Text]  
• Brewer, G. (1999). Evidence for a 3'-5' Decay Pathway for c-myc mRNA in Mammalian Cells. J. Biol. Chem. 274: 16174-16179 [Abstract] [Full Text]  
• Benard, L., Carroll, K., Valle, R. C. P., Masison, D. C., Wickner, R. B. (1999). The Ski7 Antiviral Protein Is an EF1-alpha Homolog That Blocks Expression of Non-Poly(A) mRNA in Saccharomyces cerevisiae. J. Virol. 73: 2893-2900 [Abstract] [Full Text]  
• Tharun, S., Parker, R. (1999). Analysis of Mutations in the Yeast mRNA Decapping Enzyme. Genetics 151: 1273-1285 [Abstract] [Full Text]  
• Peltz, S. W., Hammell, A. B., Cui, Y., Yasenchak, J., Puljanowski, L., Dinman, J. D. (1999). Ribosomal Protein L3 Mutants Alter Translational Fidelity and Promote Rapid Loss of the Yeast Killer Virus. Mol. Cell. Biol. 19: 384-391 [Abstract] [Full Text]  
• McCarthy, J. E. G. (1998). Posttranscriptional Control of Gene Expression in Yeast. Microbiol. Mol. Biol. Rev. 62: 1492-1553 [Abstract] [Full Text]  
• Brown, C. E., Sachs, A. B. (1998). Poly(A) Tail Length Control in Saccharomyces cerevisiae Occurs by Message-Specific Deadenylation. Mol. Cell. Biol. 18: 6548-6559 [Abstract] [Full Text]  
• Edskes, H. K., Ohtake, Y., Wickner, R. B. (1998). Mak21p of Saccharomyces cerevisiae, a Homolog of Human CAATT-binding Protein, Is Essential for 60 S Ribosomal Subunit Biogenesis. J. Biol. Chem. 273: 28912-28920 [Abstract] [Full Text]  
• Boeck, R., Lapeyre, B., Brown, C. E., Sachs, A. B. (1998). Capped mRNA Degradation Intermediates Accumulate in the Yeast spb8-2 Mutant. Mol. Cell. Biol. 18: 5062-5072 [Abstract] [Full Text]  
• Pillutla, R. C., Yue, Z., Maldonado, E., Shatkin, A. J. (1998). Recombinant Human mRNA Cap Methyltransferase Binds Capping Enzyme/RNA Polymerase IIo Complexes. J. Biol. Chem. 273: 21443-21446 [Abstract] [Full Text]  
• McNeil, J. B., Agah, H., Bentley, D. (1998). Activated transcription independent of the RNA polymerase II holoenzyme in budding yeast. Genes Dev. 12: 2510-2521 [Abstract] [Full Text]  
• Villa, T., Ceradini, F., Presutti, C., Bozzoni, I. (1998). Processing of the Intron-Encoded U18 Small Nucleolar RNA in the Yeast Saccharomyces cerevisiae Relies on Both Exo- and Endonucleolytic Activities. Mol. Cell. Biol. 18: 3376-3383 [Abstract] [Full Text]  
• Benard, L., Carroll, K., Valle, R. C. P., Wickner, R. B. (1998). Ski6p Is a Homolog of RNA-Processing Enzymes That Affects Translation of Non-Poly(A) mRNAs and 60S Ribosomal Subunit Biogenesis. Mol. Cell. Biol. 18: 2688-2696 [Abstract] [Full Text]  
• Petfalski, E., Dandekar, T., Henry, Y., Tollervey, D. (1998). Processing of the Precursors to Small Nucleolar RNAs and rRNAs Requires Common Components. Mol. Cell. Biol. 18: 1181-1189 [Abstract] [Full Text]  
• Gera, J. F., Baker, E. J. (1998). Deadenylation-Dependent and -Independent Decay Pathways for alpha 1-Tubulin mRNA in Chlamydomonas reinhardtii. Mol. Cell. Biol. 18: 1498-1505 [Abstract] [Full Text]  
• Lo, H.-J., Huang, H.-K., Donahue, T. F. (1998). RNA Polymerase I-Promoted HIS4 Expression Yields Uncapped, Polyadenylated mRNA That Is Unstable and Inefficiently Translated in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 665-675 [Abstract] [Full Text]  
• Bashkirov, V. I., Scherthan, H., Solinger, J. A., Buerstedde, J.-M., Heyer, W.-D. (1997). A Mouse Cytoplasmic Exoribonuclease (mXRN1p) with Preference for G4 Tetraplex Substrates. J. Cell Biol. 136: 761-773 [Abstract] [Full Text]  
• Gonzalez, C. I., Martin, C. E. (1996). Fatty Acid-responsive Control of mRNA Stability. UNSATURATED FATTY ACID-INDUCED DEGRADATION OF THE SACCHAROMYCES OLE1 TRANSCRIPT. J. Biol. Chem. 271: 25801-25809 [Abstract] [Full Text]  
• Caponigro, G, Parker, R (1995). Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation in yeast.. Genes Dev. 9: 2421-2432 [Abstract]  
• Stevens, A., Poole, T. L. (1995). 5`-Exonuclease-2 of Saccharomyces cerevisiae. J. Biol. Chem. 270: 16063-16069 [Abstract] [Full Text]  
• He, F, Jacobson, A (1995). Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen.. Genes Dev. 9: 437-454 [Abstract]  
• Proweller, A, Butler, S (1994). Efficient translation of poly(A)-deficient mRNAs in Saccharomyces cerevisiae.. Genes Dev. 8: 2629-2640 [Abstract]  
• Muhlrad, D, Decker, C J, Parker, R (1994). Deadenylation of the unstable mRNA encoded by the yeast MFA2 gene leads to decapping followed by 5'-->3' digestion of the transcript.. Genes Dev. 8: 855-866 [Abstract]  
• Ruiz-Echevarria, M. J., Munshi, R., Tomback, J., Kinzy, T. G., Peltz, S. W. (2001). Characterization of a General Stabilizer Element That Blocks Deadenylation-dependent mRNA Decay. J. Biol. Chem. 276: 30995-31003 [Abstract] [Full Text]  
• Jungwirth, H., Bergler, H., Hogenauer, G. (2001). Diazaborine Treatment of Baker's Yeast Results in Stabilization of Aberrant mRNAs. J. Biol. Chem. 276: 36419-36424 [Abstract] [Full Text]