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Mol Cell Biol. 1993 September; 13(9): 5225-5232

The v-Src SH3 domain binds phosphatidylinositol 3'-kinase.

X Liu, L E Marengere, C A Koch and T Pawson

Division of Molecular and Developmental Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.

ABSTRACT

Fibroblasts transformed by v-src or by related oncogenes encoding activated tyrosine kinases contain elevated levels of polyphosphoinositides with phosphate at the D-3 position of the inositol ring, as a result of the activation of phosphatidylinositol (PI) 3'-kinase. v-src-transformed cells also contain increased levels of PI 3'-kinase activity immunoprecipitable with anti-phosphotyrosine antibodies; furthermore, PI 3'-kinase can be detected in association with the v-Src tyrosine kinase. To identify regions of v-Src that can interact with PI 3'-kinase, the v-Src SH2 and SH3 domains were expressed in bacteria and incubated with lysates of normal chicken embryo fibroblasts. In vitro, the v-Src SH3 domain, but not the SH2 domain, bound PI 3'-kinase in lysates of uninfected chicken embryo fibroblasts. Substitutions of two highly conserved SH3 residues implicated in ligand binding abolished the ability of the v-Src SH3 domain to associate with PI 3'-kinase. Furthermore, the v-Src SH3 domain bound in vitro to the amino-terminal region of the p85 alpha subunit of PI 3'-kinase. These results suggest that the v-Src SH3 domain may mediate an interaction between the v-Src tyrosine kinase and PI 3'-kinase, by direct binding to p85.

Mol Cell Biol. 1993 September; 13(9): 5225-5232




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  • Jücker, M., Feldman, R. A. (1995). Identification of a New Adapter Protein That May Link the Common beta Subunit of the Receptor for Granulocyte/Macrophage Colony-stimulating Factor, Interleukin (IL)-3, and IL-5 to Phosphatidylinositol 3-Kinase. J. Biol. Chem. 270: 27817-27822 [Abstract] [Full Text]  
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  • Sudol, M., Bork, P., Einbond, A., Kastury, K., Druck, T., Negrini, M., Huebner, K., Lehman, D. (1995). Characterization of the Mammalian YAP (Yes-associated Protein) Gene and Its Role in Defining a Novel Protein Module, the WW Domain. J. Biol. Chem. 270: 14733-14741 [Abstract] [Full Text]  
  • Taylor, S. J., Anafi, M., Pawson, T., Shalloway, D. (1995). Functional Interaction between c-Src and Its Mitotic Target, Sam 68. J. Biol. Chem. 270: 10120-10124 [Abstract] [Full Text]  
  • Haefner, B., Baxter, R., Fincham, V. J., Downes, C. P., Frame, M. C. (1995). Cooperation of Src Homology Domains in the Regulated Binding of Phosphatidylinositol 3-Kinase. J. Biol. Chem. 270: 7937-7943 [Abstract] [Full Text]  
  • Chou, M. M., Hanafusa, H. (1995). A Novel Ligand for SH3 Domains. J. Biol. Chem. 270: 7359-7364 [Abstract] [Full Text]  
  • Abrams, C. S., Zhao, W. (1995). SH3 Domains Specifically Regulate Kinase Activity of Expressed Src Family Proteins. J. Biol. Chem. 270: 333-339 [Abstract] [Full Text]  
  • Feng, S, Chen, J., Yu, H, Simon, J., Schreiber, S. (1994). Two binding orientations for peptides to the Src SH3 domain: development of a general model for SH3-ligand interactions. Science 266: 1241-1247 [Abstract]  
  • Ren, R, Ye, Z S, Baltimore, D (1994). Abl protein-tyrosine kinase selects the Crk adapter as a substrate using SH3-binding sites.. Genes Dev. 8: 783-795 [Abstract]  
  • Pleiman, C., Hertz, W., Cambier, J. (1994). Activation of phosphatidylinositol-3' kinase by Src-family kinase SH3 binding to the p85 subunit. Science 263: 1609-1612 [Abstract]  
  • Birge, R., Hanafusa, H (1993). Closing in on SH2 specificity. Science 262: 1522-1524  
  • Hauck, C. R., Hunter, T., Schlaepfer, D. D. (2001). The v-Src SH3 Domain Facilitates a Cell Adhesion-independent Association with Focal Adhesion Kinase. J. Biol. Chem. 276: 17653-17662 [Abstract] [Full Text]  
  • Stam, J. C., Geerts, W. J. C., Versteeg, H. H., Verkleij, A. J., en Henegouwen, P. M. P. v. B. (2001). The v-Crk Oncogene Enhances Cell Survival and Induces Activation of Protein Kinase B/Akt. J. Biol. Chem. 276: 25176-25183 [Abstract] [Full Text]  


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