This Article Full Text (PDF) A correction has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Deng, W P Articles by Nickoloff, J A Search for Related Content PubMed PubMed Citation Articles by Deng, W P Articles by Nickoloff, J A

Mismatch repair of heteroduplex DNA intermediates of extrachromosomal recombination in mammalian cells.

Department of Cancer Biology, Harvard University School of Public Health, Boston, Massachusetts 02115.

ABSTRACT

Previous work indicated that extrachromosomal recombination in mammalian cells could be explained by the single-strand annealing (SSA) model. This model predicts that extrachromosomal recombination leads to nonconservative crossover products and that heteroduplex DNA (hDNA) is formed by annealing of complementary single strands. Mismatched bases in hDNA may subsequently be repaired to wild-type or mutant sequences, or they may remain unrepaired and segregate following DNA replication. We describe a system to examine the formation and mismatch repair of hDNA in recombination intermediates. Our results are consistent with extrachromosomal recombination occurring via SSA and producing crossover recombinant products. As predicted by the SSA model, hDNA was present in double-strand break-induced recombination intermediates. By placing either silent or frameshift mutations in the predicted hDNA region, we have shown that mismatches are efficiently repaired prior to DNA replication.

This article has been cited by other articles:

• Preston, C. R., Engels, W., Flores, C. (2002). Efficient Repair of DNA Breaks in Drosophila: Evidence for Single-Strand Annealing and Competition With Other Repair Pathways. Genetics 161: 711-720 [Abstract] [Full Text]  
• Corrette-Bennett, S. E., Mohlman, N. L., Rosado, Z., Miret, J. J., Hess, P. M., Parker, B. O., Lahue, R. S. (2001). Efficient repair of large DNA loops in Saccharomyces cerevisiae. Nucleic Acids Res 29: 4134-4143 [Abstract] [Full Text]  
• Faruqi, A. F., Datta, H. J., Carroll, D., Seidman, M. M., Glazer, P. M. (2000). Triple-Helix Formation Induces Recombination in Mammalian Cells via a Nucleotide Excision Repair-Dependent Pathway. Mol. Cell. Biol. 20: 990-1000 [Abstract] [Full Text]  
• Corrette-Bennett, S. E., Parker, B. O., Mohlman, N. L., Lahue, R. S. (1999). Correction of Large Mispaired DNA Loops by Extracts of Saccharomyces cerevisiae. J. Biol. Chem. 274: 17605-17611 [Abstract] [Full Text]  
• Ng, P., Baker, M. D. (1999). Mechanisms of Double-Strand-Break Repair During Gene Targeting in Mammalian Cells. Genetics 151: 1127-1141 [Abstract] [Full Text]  
• Bill, C. A., Duran, W. A., Miselis, N. R., Nickoloff, J. A. (1998). Efficient Repair of All Types of Single-Base Mismatches in Recombination Intermediates in Chinese Hamster Ovary Cells: Competition Between Long-Patch and G-T Glycosylase-Mediated Repair of G-T Mismatches. Genetics 149: 1935-1943 [Abstract] [Full Text]  
• Taghian, D. G., Hough, H., Nickoloff, J. A. (1998). Biased Short Tract Repair of Palindromic Loop Mismatches in Mammalian Cells. Genetics 148: 1257-1268 [Abstract] [Full Text]