This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Diehl, J A Articles by Hannink, M Search for Related Content PubMed PubMed Citation Articles by Diehl, J A Articles by Hannink, M

Identification of a C/EBP-Rel complex in avian lymphoid cells.

Biochemistry Department, University of Missouri, Columbia 65212.

ABSTRACT

Protein-protein interactions between the CCAAT box enhancer-binding proteins (C/EBP) and the Rel family of transcription factors have been implicated in the regulation of cytokine gene expression. We have used sequence-specific DNA affinity chromatography to purify a complex from avian T cells that binds to a consensus C/EBP motif. Our results provide evidence that Rel-related proteins are components of the C/EBP-DNA complex as a result of protein-protein interactions with the C/EBP proteins. A polyclonal antiserum raised against the Rel homology domain of v-Rel and antisera raised against two human RelA-derived peptides specifically induced a supershift of the C/EBP-DNA complex in mobility shift assays using the affinity-purified C/EBP. In addition, several kappa B-binding proteins copurified with the avian C/EBP complex through two rounds of sequence-specific DNA affinity chromatography. The kappa B-binding proteins are distinct from the C/EBP proteins that directly contact DNA containing the C/EBP binding site. The identification of a protein complex that binds specifically to a consensus C/EBP site and contains both C/EBP and Rel family members suggests a novel mechanism for regulation of gene expression by Rel family proteins.

This article has been cited by other articles:

• Chumakov, A. M., Silla, A., Williamson, E. A., Koeffler, H. P. (2007). Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with NFkappaB pathway. Blood 109: 4209-4219 [Abstract] [Full Text]  
• Chen, J., Zhao, M., Rao, R., Inoue, H., Hao, C.-M. (2005). C/EBP{beta} and Its Binding Element Are Required for NF{kappa}B-induced COX2 Expression Following Hypertonic Stress. J. Biol. Chem. 280: 16354-16359 [Abstract] [Full Text]  
• Burkart, A. D., Mukherjee, A., Sterneck, E., Johnson, P. F., Mayo, K. E. (2005). Repression of the Inhibin {alpha}-Subunit Gene by the Transcription Factor CCAAT/Enhancer-Binding Protein-{beta}. Endocrinology 146: 1909-1921 [Abstract] [Full Text]  
• Schrem, H., Klempnauer, J., Borlak, J. (2004). Liver-Enriched Transcription Factors in Liver Function and Development. Part II: the C/EBPs and D Site-Binding Protein in Cell Cycle Control, Carcinogenesis, Circadian Gene Regulation, Liver Regeneration, Apoptosis, and Liver-Specific Gene Regulation. Pharmacol. Rev. 56: 291-330 [Abstract] [Full Text]  
• Bradley, M. N., Zhou, L., Smale, S. T. (2003). C/EBP{beta} Regulation in Lipopolysaccharide-Stimulated Macrophages. Mol. Cell. Biol. 23: 4841-4858 [Abstract] [Full Text]  
• Edelstein, L. C., Lagos, L., Simmons, M., Tirumalai, H., Gelinas, C. (2003). NF-{kappa}B-Dependent Assembly of an Enhanceosome-Like Complex on the Promoter Region of Apoptosis Inhibitor Bfl-1/A1. Mol. Cell. Biol. 23: 2749-2761 [Abstract] [Full Text]  
• Lai, C.-K., Ting, L.-P. (1999). Transcriptional Repression of Human Hepatitis B Virus Genes by a bZIP Family Member, E4BP4. J. Virol. 73: 3197-3209 [Abstract] [Full Text]  
• Montaner, S., Perona, R., Saniger, L., Lacal, J. C. (1999). Activation of Serum Response Factor by RhoA Is Mediated by the Nuclear Factor-kappa B and C/EBP Transcription Factors. J. Biol. Chem. 274: 8506-8515 [Abstract] [Full Text]  
• Bauknecht, T., Shi, Y. (1998). Overexpression of C/EBPbeta Represses Human Papillomavirus Type 18 Upstream Regulatory Region Activity in HeLa Cells by Interfering with the Binding of TATA-Binding Protein. J. Virol. 72: 2113-2124 [Abstract] [Full Text]  
• Tenen, D. G., Hromas, R., Licht, J. D., Zhang, D.-E. (1997). Transcription Factors, Normal Myeloid Development, and Leukemia. Blood 90: 489-519 [Full Text]  
• Cogswell, P. C., Mayo, M. W., Baldwin, A. S. Jr. (1997). Involvement of Egr-1/RelA Synergy in Distinguishing T Cell Activation from Tumor Necrosis Factor-alpha -induced NF-kappa B1 Transcription. J. Exp. Med. 185: 491-498 [Abstract] [Full Text]  
• Martin, M. E., Chinenov, Y., Yu, M., Schmidt, T. K., Yang, X.-Y. (1996). Redox Regulation of GA-binding Protein-alpha DNA Binding Activity. J. Biol. Chem. 271: 25617-25623 [Abstract] [Full Text]  
• Vietor, I., Oliveira, I. C., Vilcek, J. (1996). CCAAT Box Enhancer Binding Protein alpha (C/EBP-alpha) Stimulates kappaB Element-mediated Transcription in Transfected Cells. J. Biol. Chem. 271: 5595-5602 [Abstract] [Full Text]  
• Doppler, W., Welte, T., Philipp, S. (1995). CCAAT/Enhancer-binding Protein Isoforms [IMAGE] and [IMAGE] Are Expressed in Mammary Epithelial Cells and Bind to Multiple Sites in the [IMAGE]-Casein Gene Promoter. J. Biol. Chem. 270: 17962-17969 [Abstract] [Full Text]  
• Diehl, J. A., Tong, W., Sun, G., Hannink, M. (1995). Tumor Necrosis Factor-alpha-dependent Activation of a RelA Homodimer in Astrocytes. J. Biol. Chem. 270: 2703-2707 [Abstract] [Full Text]  
• Massaad, C., Paradon, M., Jacques, C., Salvat, C., Bereziat, G., Berenbaum, F., Olivier, J.-L. (2000). Induction of Secreted Type IIA Phospholipase A2 Gene Transcription by Interleukin-1beta . ROLE OF C/EBP FACTORS. J. Biol. Chem. 275: 22686-22694 [Abstract] [Full Text]