This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhong, W
Right arrow Articles by Darnell, J E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhong, W
Right arrow Articles by Darnell, J E, Jr

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 November; 14(11): 7276-7284

Tissue-specific regulation of mouse hepatocyte nuclear factor 4 expression.

W Zhong, J Mirkovitch and J E Darnell Jr

Laboratory of Molecular Cell Biology, Rockefeller University, New York, New York 10021.

ABSTRACT

Hepatocyte nuclear factor 4 (HNF-4) is a liver-enriched transcription factor and a member of the steroid hormone receptor superfamily. HNF-4 is required for the hepatoma-specific expression of HNF-1 alpha, another liver-enriched transcription factor, suggesting the early participation of HNF-4 in development. To prepare for further study of HNF-4 in development, the tissue-specific expression of the mouse HNF-4 gene was studied by analyzing the promoter region for required DNA elements. DNase-hypersensitive sites in the gene in liver and kidney tissues were found in regions both distal and proximal to the RNA start that were absent in tissues in which HNF-4 expression did not occur. By use of reporter constructs in transient-transfection assays and with transgenic mice, a region sufficient to drive liver-specific expression of HNF-4 was identified. While an HNF-1 binding site between bp -98 and -68 played an important role in the hepatoma-specific promoter activity of HNF-4 in transient-transfection assays, it was not sufficient for the liver-specific expression of a reporter gene in transgenic mice. Distal enhancer elements indicated by the presence of DNase I-hypersensitive sites at kb -5.5 and -6.5, while not functional in transient-transfection assays, were required for the correct expression of the mouse HNF-4 gene in animals.

Mol Cell Biol. 1994 November; 14(11): 7276-7284




This article has been cited by other articles:

  • Leng, S., Lu, S., Yao, Y., Kan, Z., Morris, G. S., Stair, B. R., Cherny, M. A., Black, D. D. (2007). Hepatocyte nuclear factor-4 mediates apolipoprotein A-IV transcriptional regulation by fatty acid in newborn swine enterocytes. Am. J. Physiol. Gastrointest. Liver Physiol. 293: G475-G483 [Abstract] [Full Text]  
  • Barbacci, E., Chalkiadaki, A., Masdeu, C., Haumaitre, C., Lokmane, L., Loirat, C., Cloarec, S., Talianidis, I., Bellanne-Chantelot, C., Cereghini, S. (2004). HNF1{beta}/TCF2 mutations impair transactivation potential through altered co-regulator recruitment. Hum Mol Genet 13: 3139-3149 [Abstract] [Full Text]  
  • Briancon, N., Bailly, A., Clotman, F., Jacquemin, P., Lemaigre, F. P., Weiss, M. C. (2004). Expression of the {alpha}7 Isoform of Hepatocyte Nuclear Factor (HNF) 4 Is Activated by HNF6/OC-2 and HNF1 and Repressed by HNF4{alpha}1 in the Liver. J. Biol. Chem. 279: 33398-33408 [Abstract] [Full Text]  
  • Massillon, D., Arinze, I. J., Xu, C., Bone, F. (2003). Regulation of Glucose-6-phosphatase Gene Expression in Cultured Hepatocytes and H4IIE Cells by Short-chain Fatty Acids: ROLE OF HEPATIC NUCLEAR FACTOR-4{alpha}. J. Biol. Chem. 278: 40694-40701 [Abstract] [Full Text]  
  • Haumaitre, C., Reber, M., Cereghini, S. (2003). Functions of HNF1 Family Members in Differentiation of the Visceral Endoderm Cell Lineage. J. Biol. Chem. 278: 40933-40942 [Abstract] [Full Text]  
  • Lin, W.-J., Li, J., Lee, Y.-F., Yeh, S.-D., Altuwaijri, S., Ou, J.-H., Chang, C. (2003). Suppression of Hepatitis B Virus Core Promoter by the Nuclear Orphan Receptor TR4. J. Biol. Chem. 278: 9353-9360 [Abstract] [Full Text]  
  • Schrem, H., Klempnauer, J., Borlak, J. (2002). Liver-Enriched Transcription Factors in Liver Function and Development. Part I: The Hepatocyte Nuclear Factor Network and Liver-Specific Gene Expression. Pharmacol. Rev. 54: 129-158 [Abstract] [Full Text]  
  • Guo, H., Cai, C. Q., Kuo, P. C. (2002). Hepatocyte Nuclear Factor-4alpha Mediates Redox Sensitivity of Inducible Nitric-oxide Synthase Gene Transcription. J. Biol. Chem. 277: 5054-5060 [Abstract] [Full Text]  
  • Shih, D. Q., Stoffel, M. (2001). Dissecting the transcriptional network of pancreatic islets during development and differentiation. Proc. Natl. Acad. Sci. USA 98: 14189-14191 [Full Text]  
  • Hatzis, P., Talianidis, I. (2001). Regulatory Mechanisms Controlling Human Hepatocyte Nuclear Factor 4{alpha} Gene Expression. Mol. Cell. Biol. 21: 7320-7330 [Abstract] [Full Text]  
  • Thomas, H., Jaschkowitz, K., Bulman, M., Frayling, T. M., Mitchell, S. M.S., Roosen, S., Lingott-Frieg, A., Tack, C. J., Ellard, S., Ryffel, G. U., Hattersley, A. T. (2001). A distant upstream promoter of the HNF-4{alpha} gene connects the transcription factors involved in maturity-onset diabetes of the young. Hum Mol Genet 10: 2089-2097 [Abstract] [Full Text]  
  • Bailly, A., Torres-Padilla, M. E., Tinel, A. P., Weiss, M. C. (2001). An enhancer element 6 kb upstream of the mouse HNF4{alpha}1 promoter is activated by glucocorticoids and liver-enriched transcription factors. Nucleic Acids Res 29: 3495-3505 [Abstract] [Full Text]  
  • Peiler, G., Böckmann, B., Nakhei, H., Ryffel, G. U. (2000). Inhibitor of the Tissue-Specific Transcription Factor HNF4, a Potential Regulator in Early Xenopus Development. Mol. Cell. Biol. 20: 8676-8683 [Abstract] [Full Text]  
  • Lausen, J., Thomas, H., Lemm, I., Bulman, M., Borgschulze, M., Lingott, A., Hattersley, A. T., Ryffel, G. U. (2000). Naturally occurring mutations in the human HNF4{alpha} gene impair the function of the transcription factor to a varying degree. Nucleic Acids Res 28: 430-437 [Abstract] [Full Text]  
  • Stroup, D., Chiang, J. Y. L. (2000). HNF4 and COUP-TFII interact to modulate transcription of the cholesterol 7{alpha}-hydroxylase gene (CYP7A1). J. Lipid Res. 41: 1-11 [Abstract] [Full Text]  
  • Rollini, P., Fournier, R. E. K. (1999). The HNF-4/HNF-1alpha transactivation cascade regulates gene activity and chromatin structure of the human serine protease inhibitor gene cluster at 14q32.1. Proc. Natl. Acad. Sci. USA 96: 10308-10313 [Abstract] [Full Text]  
  • Barbacci, E, Reber, M, Ott, M., Breillat, C, Huetz, F, Cereghini, S (1999). Variant hepatocyte nuclear factor 1 is required for visceral endoderm specification. Development 126: 4795-4805 [Abstract]  
  • Kapitskaya, M. Z., Dittmer, N. T., Deitsch, K. W., Cho, W.-L., Taylor, D. G., Leff, T., Raikhel, A. S. (1998). Three Isoforms of a Hepatocyte Nuclear Factor-4 Transcription Factor with Tissue- and Stage-specific Expression in the Adult Mosquito. J. Biol. Chem. 273: 29801-29810 [Abstract] [Full Text]  
  • Magee, T. R., Cai, Y., El-Houseini, M. E., Locker, J., Wan, Y.-J. Y. (1998). Retinoic Acid Mediates Down-regulation of the alpha -Fetoprotein Gene through Decreased Expression of Hepatocyte Nuclear Factors. J. Biol. Chem. 273: 30024-30032 [Abstract] [Full Text]  
  • Farsetti, A., Moretti, F., Narducci, M., Misiti, S., Nanni, S., Andreoli, M., Sacchi, A., Pontecorvi, A. (1998). Orphan Receptor Hepatocyte Nuclear Factor-4 Antagonizes Estrogen Receptor {alpha}-Mediated Induction of Human Coagulation Factor XII Gene. Endocrinology 139: 4581-4589 [Abstract] [Full Text]  
  • Duncan, S. A., Navas, M. A., Dufort, D., Rossant, J., Stoffel, M. (1998). Regulation of a Transcription Factor Network Required for Differentiation and Metabolism. Science 281: 692-695 [Abstract] [Full Text]  
  • Stauffer, D. R., Chukwumezie, B. N., Wilberding, J. A., Rosen, E. D., Castellino, F. J. (1998). Characterization of Transcriptional Regulatory Elements in the Promoter Region of the Murine Blood Coagulation Factor VII Gene. J. Biol. Chem. 273: 2277-2287 [Abstract] [Full Text]  
  • Bailly, A, Spath, G, Bender, V, Weiss, M. (1998). Phenotypic effects of the forced expression of HNF4 and HNF1alpha are conditioned by properties of the recipient cell. J. Cell Sci. 111: 2411-2421 [Abstract]  
  • Scholz, H., Bossone, S. A., Cohen, H. T., Akella, U., Strauss, W. M., Sukhatme, V. P. (1997). A Far Upstream Cis-element Is Required for Wilms' Tumor-1 (WT1) Gene Expression in Renal Cell Culture. J. Biol. Chem. 272: 32836-32846 [Abstract] [Full Text]  
  • He, C.-Z., Burch, J. B. E. (1997). The Chicken GATA-6 Locus Contains Multiple Control Regions That Confer Distinct Patterns of Heart Region-specific Expression in Transgenic Mouse Embryos. J. Biol. Chem. 272: 28550-28556 [Abstract] [Full Text]  
  • Lim, L., Zhou, H., Costa, R. H. (1997). The winged helix transcription factor HFH-4 is expressed during choroid plexus epithelial development in the mouse embryo. Proc. Natl. Acad. Sci. USA 94: 3094-3099 [Abstract] [Full Text]  
  • Zhong, W, Jiang, M., Weinmaster, G, Jan, L., Jan, Y. (1997). Differential expression of mammalian Numb, Numblike and Notch1 suggests distinct roles during mouse cortical neurogenesis. Development 124: 1887-1897 [Abstract]  
  • Pare, J.-F., Roy, S., Galarneau, L., Belanger, L. (2001). The Mouse Fetoprotein Transcription Factor (FTF) Gene Promoter Is Regulated by Three GATA Elements with Tandem E Box and Nkx Motifs, and FTF in Turn Activates the Hnf3beta , Hnf4alpha , and Hnf1alpha Gene Promoters. J. Biol. Chem. 276: 13136-13144 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»