This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cvekl, A
Right arrow Articles by Piatigorsky, J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cvekl, A
Right arrow Articles by Piatigorsky, J

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 November; 14(11): 7363-7376

A complex array of positive and negative elements regulates the chicken alpha A-crystallin gene: involvement of Pax-6, USF, CREB and/or CREM, and AP-1 proteins.

A Cvekl, C M Sax, E H Bresnick and J Piatigorsky

Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-2730.

ABSTRACT

The abundance of crystallins (> 80% of the soluble protein) in the ocular lens provides advantageous markers for selective gene expression during cellular differentiation. Here we show by functional and protein-DNA binding experiments that the chicken alpha A-crystallin gene is regulated by at least five control elements located at sites A (-148 to -139), B (-138 to -132), C (-128 to -101), D (-102 to -93), and E (-56 to -41). Factors interacting with these sites were characterized immunologically and by gel mobility shift experiments. The results are interpreted with the following model. Site A binds USF and is part of a composite element with site B. Site B binds CREB and/or CREM to enhance expression in the lens and binds an AP-1 complex including CREB, Fra2 and/or JunD which interacts with USF on site A to repress expression in fibroblasts. Sites C and E (which is conserved across species) bind Pax-6 in the lens to stimulate alpha A-crystallin promoter activity. These experiments provide the first direct data that Pax-6 contributes to the lens-specific expression of a crystallin gene. Site D (-104 to -93) binds USF and is a negative element. Thus, the data indicate that USF, CREB and/or CREM (or AP-1 factors), and Pax-6 bind a complex array of positive and negative cis-acting elements of the chicken alpha A-crystallin gene to control high expression in the lens and repression in fibroblasts.

Mol Cell Biol. 1994 November; 14(11): 7363-7376




This article has been cited by other articles:

  • Ciccone, N. A., Lacza, C. T., Hou, M. Y., Gregory, S. J., Kam, K.-Y., Xu, S., Kaiser, U. B. (2008). A Composite Element that Binds Basic Helix Loop Helix and Basic Leucine Zipper Transcription Factors Is Important for Gonadotropin-Releasing Hormone Regulation of the Follicle-Stimulating Hormone {beta} Gene. Mol. Endocrinol. 22: 1908-1923 [Abstract] [Full Text]  
  • Davis, J., Davis, D., Norman, B., Piatigorsky, J. (2008). Gene Expression of the Mouse Corneal Crystallin Aldh3a1: Activation by Pax6, Oct1, and p300. IOVS 49: 1814-1826 [Abstract] [Full Text]  
  • Yan, Q., Liu, W.-B., Qin, J., Liu, J., Chen, H.-G., Huang, X., Chen, L., Sun, S., Deng, M., Gong, L., Li, Y., Zhang, L., Liu, Y., Feng, H., Xiao, Y., Liu, Y., Li, D. W.-C. (2007). Protein Phosphatase-1 Modulates the Function of Pax-6, a Transcription Factor Controlling Brain and Eye Development. J. Biol. Chem. 282: 13954-13965 [Abstract] [Full Text]  
  • Steiger, J. L., Bandyopadhyay, S., Farb, D. H., Russek, S. J. (2004). cAMP Response Element-Binding Protein, Activating Transcription Factor-4, and Upstream Stimulatory Factor Differentially Control Hippocampal GABABR1a and GABABR1b Subunit Gene Expression through Alternative Promoters. J. Neurosci. 24: 6115-6126 [Abstract] [Full Text]  
  • Zhao, H., Yang, Y., Rizo, C. M., Overbeek, P. A., Robinson, M. L. (2004). Insertion of a Pax6 Consensus Binding Site into the {alpha}A-Crystallin Promoter Acts as a Lens Epithelial Cell Enhancer in Transgenic Mice. IOVS 45: 1930-1939 [Abstract] [Full Text]  
  • Cui, W., Tomarev, S. I., Piatigorsky, J., Chepelinsky, A. B., Duncan, M. K. (2004). Mafs, Prox1, and Pax6 Can Regulate Chicken {beta}B1-Crystallin Gene Expression. J. Biol. Chem. 279: 11088-11095 [Abstract] [Full Text]  
  • Piatigorsky, J. (2003). Gene Sharing, Lens Crystallins and Speculations on an Eye/Ear Evolutionary Relationship. Integr. Comp. Biol. 43: 492-499 [Abstract] [Full Text]  
  • Davis, J., Duncan, M. K., Robison, W. G. Jr, Piatigorsky, J. (2003). Requirement for Pax6 in corneal morphogenesis: a role in adhesion. J. Cell Sci. 116: 2157-2167 [Abstract] [Full Text]  
  • Weston, C. R., Wong, A., Hall, J. P., Goad, M. E.P., Flavell, R. A., Davis, R. J. (2003). JNK initiates a cytokine cascade that causes Pax2 expression and closure of the optic fissure. Genes Dev. 17: 1271-1280 [Abstract] [Full Text]  
  • Chen, W. G., West, A. E., Tao, X., Corfas, G., Szentirmay, M. N., Sawadogo, M., Vinson, C., Greenberg, M. E. (2003). Upstream Stimulatory Factors Are Mediators of Ca2+-Responsive Transcription in Neurons. J. Neurosci. 23: 2572-2581 [Abstract] [Full Text]  
  • Chauhan, B. K., Zhang, W., Cveklova, K., Kantorow, M., Cvekl, A. (2002). Identification of Differentially Expressed Genes in Mouse Pax6 Heterozygous Lenses. IOVS 43: 1884-1890 [Abstract] [Full Text]  
  • Xing, W., Danilovich, N., Sairam, M. R. (2002). Orphan Receptor Chicken Ovalbumin Upstream Promoter Transcription Factors Inhibit Steroid Factor-1, Upstream Stimulatory Factor, and Activator Protein-1 Activation of Ovine Follicle-Stimulating Hormone Receptor Expression via Composite cis-Elements. Biol. Reprod. 66: 1656-1666 [Abstract] [Full Text]  
  • Yang, X.-P., Freeman, L. A., Knapper, C. L., Amar, M. J. A., Remaley, A., Brewer, H. B. Jr., Santamarina-Fojo, S. (2002). The E-box motif in the proximal ABCA1 promoter mediates transcriptional repression of the ABCA1 gene. J. Lipid Res. 43: 297-306 [Abstract] [Full Text]  
  • Carosa, E., Kozmik, Z., Rall, J. E., Piatigorsky, J. (2002). Structure and Expression of the Scallop Omega -Crystallin Gene. EVIDENCE FOR CONVERGENT EVOLUTION OF PROMOTER SEQUENCES. J. Biol. Chem. 277: 656-664 [Abstract] [Full Text]  
  • Sun, H., Dickinson, D. P., Costello, J., Li, W.-H. (2001). Isolation of Cladonema Pax-B Genes and Studies of the DNA-Binding Properties of Cnidarian Pax Paired Domains. Mol Biol Evol 18: 1905-1918 [Abstract] [Full Text]  
  • Vereshchagina, L. A., Tolnay, M., Tsokos, G. C. (2001). Multiple Transcription Factors Regulate the Inducible Expression of the Human Complement Receptor 2 Promoter. J. Immunol. 166: 6156-6163 [Abstract] [Full Text]  
  • Duncan, M., Kozmik, Z, Cveklova, K, Piatigorsky, J, Cvekl, A (2000). Overexpression of PAX6(5a) in lens fiber cells results in cataract and upregulation of (alpha)5(beta)1 integrin expression. J. Cell Sci. 113: 3173-3185 [Abstract]  
  • Rich, C. B., Fontanilla, M. R., Nugent, M., Foster, J. A. (1999). Basic Fibroblast Growth Factor Decreases Elastin Gene Transcription through an AP1/cAMP-response Element Hybrid Site in the Distal Promoter. J. Biol. Chem. 274: 33433-33439 [Abstract] [Full Text]  
  • Ilagan, J. G., Cvekl, A., Kantorow, M., Piatigorsky, J., Sax, C. M. (1999). Regulation of alpha A-crystallin Gene Expression. LENS SPECIFICITY ACHIEVED THROUGH THE DIFFERENTIAL PLACEMENT OF SIMILAR TRANSCRIPTIONAL CONTROL ELEMENTS IN MOUSE AND CHICKEN. J. Biol. Chem. 274: 19973-19978 [Abstract] [Full Text]  
  • Cammarata, P. R., Zhou, C., Chen, G., Singh, I., Reeves, R. E., Kuszak, J. R., Robinson, M. L. (1999). A Transgenic Animal Model of Osmotic Cataract. Part 1: Over-expression of Bovine Na+/Myo-inositol Cotransporter in Lens Fibers. IOVS 40: 1727-1737 [Abstract] [Full Text]  
  • Niimi, T, Seimiya, M, Kloter, U, Flister, S, Gehring, W. (1999). Direct regulatory interaction of the eyeless protein with an eye-specific enhancer in the sine oculis gene during eye induction in Drosophila. Development 126: 2253-2260 [Abstract]  
  • Duncan, M. K., Haynes, J. I. II, Cvekl, A., Piatigorsky, J. (1998). Dual Roles for Pax-6: a Transcriptional Repressor of Lens Fiber Cell-Specific beta -Crystallin Genes. Mol. Cell. Biol. 18: 5579-5586 [Abstract] [Full Text]  
  • Gopal-Srivastava, R., Cvekl, A., Piatigorsky, J. (1998). Involvement of Retinoic Acid/Retinoid Receptors in the Regulation of Murine alpha B-crystallin/Small Heat Shock Protein Gene Expression in the Lens. J. Biol. Chem. 273: 17954-17961 [Abstract] [Full Text]  
  • Kays, W. T., Piatigorsky, J. (1997). Aldehyde dehydrogenase class 3 expression: Identification of a cornea-preferred gene promoter in transgenic mice. Proc. Natl. Acad. Sci. USA 94: 13594-13599 [Abstract] [Full Text]  
  • Gao, E., Wang, Y., Alcorn, J. L., Mendelson, C. R. (1997). The Basic Helix-Loop-Helix-Zipper Transcription Factor USF1 Regulates Expression of the Surfactant Protein-A Gene. J. Biol. Chem. 272: 23398-23406 [Abstract] [Full Text]  
  • Rippe, R. A., Umezawa, A., Kimball, J. P., Breindl, M., Brenner, D. A. (1997). Binding of Upstream Stimulatory Factor to an E-box in the 3'-Flanking Region Stimulates alpha 1(I) Collagen Gene Transcription. J. Biol. Chem. 272: 1753-1760 [Abstract] [Full Text]  
  • Cippitelli, M., Ye, J., Viggiano, V., Sica, A., Ghosh, P., Gulino, A., Santoni, A., Young, H. A. (1996). Retinoic Acid-induced Transcriptional Modulation of the Human Interferon-gamma Promoter. J. Biol. Chem. 271: 26783-26793 [Abstract] [Full Text]  
  • Gopal-Srivastava, R., Cvekl, A., Piatigorsky, J. (1996). Pax-6 and alpha B-crystallin/Small Heat Shock Protein Gene Regulation in the Murine Lens. INTERACTION WITH THE LENS-SPECIFIC REGIONS, LSR1 AND LSR2. J. Biol. Chem. 271: 23029-23036 [Abstract] [Full Text]  
  • Frederikse, P. H., Garland, D., Zigler, J. S. Jr., Piatigorsky, J. (1996). Oxidative Stress Increases Production of beta-Amyloid Precursor Protein and beta-Amyloid (Abeta) in Mammalian Lenses, and Abeta Has Toxic Effects on Lens Epithelial Cells. J. Biol. Chem. 271: 10169-10174 [Abstract] [Full Text]  
  • Viollet, B.ît, Lefrançois-Martinez, A.-M., Henrion, A., Kahn, A., Raymondjean, M., Martinez, A. (1996). Immunochemical Characterization and Transacting Properties of Upstream Stimulatory Factor Isoforms. J. Biol. Chem. 271: 1405-1415 [Abstract] [Full Text]  
  • Harris, V. K., Coticchia, C. M., List, H.-J., Wellstein, A., Riegel, A. T. (2000). Mitogen-induced Expression of the Fibroblast Growth Factor-binding Protein Is Transcriptionally Repressed through a Non-canonical E-box Element. J. Biol. Chem. 275: 28539-28548 [Abstract] [Full Text]  
  • Kataoka, K., Yoshitomo-Nakagawa, K., Shioda, S., Nishizawa, M. (2001). A Set of Hox Proteins Interact with the Maf Oncoprotein to Inhibit Its DNA Binding, Transactivation, and Transforming Activities. J. Biol. Chem. 276: 819-826 [Abstract] [Full Text]  
  • Planque, N., Leconte, L., Coquelle, F. M., Benkhelifa, S., Martin, P., Felder-Schmittbuhl, M.-P., Saule, S. (2001). Interaction of Maf Transcription Factors with Pax-6 Results in Synergistic Activation of the Glucagon Promoter. J. Biol. Chem. 276: 35751-35760 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»