This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ghivizzani, S C Articles by Hauswirth, W W Search for Related Content PubMed PubMed Citation Articles by Ghivizzani, S C Articles by Hauswirth, W W

In organello footprint analysis of human mitochondrial DNA: human mitochondrial transcription factor A interactions at the origin of replication.

Department of Immunology and Medical Microbiology, University of Florida, College of Medicine, Gainesville 32610-0266.

ABSTRACT

Using in organello footprint analysis, we demonstrate that within human placental mitochondria there is a high level of protein-DNA binding at regularly phased intervals throughout a 500-bp region encompassing the D-loop DNA origins and two promoter regions. Comparison with in vitro DNase I protection studies indicates that this protein-DNA interaction is due to non-sequence-specific binding by human mitochondrial transcription factor A (h-mtTFA). Since h-mtTFA can bend and wrap DNA, like its yeast counterpart ABF2, a primary function of h-mtTFA appears to be specific packaging of the mitochondrial DNA control region in vivo. Intervals of protein binding coincide with the spacing of the RNA start sites and prominent D-loop DNA 5' ends, suggesting a role for phased h-mtTFA binding in defining transcription and H-strand DNA replication origins. Significant protein-DNA interaction was also observed within the human homolog of conserved sequence block 1, both in organello and in vitro, using purified h-mtTFA.

This article has been cited by other articles:

• Kaufman, B. A., Durisic, N., Mativetsky, J. M., Costantino, S., Hancock, M. A., Grutter, P., Shoubridge, E. A. (2007). The Mitochondrial Transcription Factor TFAM Coordinates the Assembly of Multiple DNA Molecules into Nucleoid-like Structures. Mol. Biol. Cell 18: 3225-3236 [Abstract] [Full Text]  
• Ohgaki, K., Kanki, T., Fukuoh, A., Kurisaki, H., Aoki, Y., Ikeuchi, M., Kim, S. H., Hamasaki, N., Kang, D. (2007). The C-terminal Tail of Mitochondrial Transcription Factor A Markedly Strengthens its General Binding to DNA. J Biochem 141: 201-211 [Abstract] [Full Text]  
• Pohjoismaki, J. L. O., Wanrooij, S., Hyvarinen, A. K., Goffart, S., Holt, I. J., Spelbrink, J. N., Jacobs, H. T. (2006). Alterations to the expression level of mitochondrial transcription factor A, TFAM, modify the mode of mitochondrial DNA replication in cultured human cells. Nucleic Acids Res 34: 5815-5828 [Abstract] [Full Text]  
• Hudson, G., Chinnery, P. F. (2006). Mitochondrial DNA polymerase-{gamma} and human disease. Hum Mol Genet 15: R244-R252 [Abstract] [Full Text]  
• Kuroda, Y., Mitsui, T., Kunishige, M., Shono, M., Akaike, M., Azuma, H., Matsumoto, T. (2006). Parkin enhances mitochondrial biogenesis in proliferating cells. Hum Mol Genet 15: 883-895 [Abstract] [Full Text]  
• Matsushima, Y., Adan, C., Garesse, R., Kaguni, L. S. (2005). Drosophila Mitochondrial Transcription Factor B1 Modulates Mitochondrial Translation but Not Transcription or DNA Copy Number in Schneider Cells. J. Biol. Chem. 280: 16815-16820 [Abstract] [Full Text]  
• Maniura-Weber, K., Goffart, S., Garstka, H. L., Montoya, J., Wiesner, R. J. (2004). Transient overexpression of mitochondrial transcription factor A (TFAM) is sufficient to stimulate mitochondrial DNA transcription, but not sufficient to increase mtDNA copy number in cultured cells. Nucleic Acids Res 32: 6015-6027 [Abstract] [Full Text]  
• Matsushima, Y., Garesse, R., Kaguni, L. S. (2004). Drosophila Mitochondrial Transcription Factor B2 Regulates Mitochondrial DNA Copy Number and Transcription in Schneider Cells. J. Biol. Chem. 279: 26900-26905 [Abstract] [Full Text]  
• Wanrooij, S., Luoma, P., van Goethem, G., van Broeckhoven, C., Suomalainen, A., Spelbrink, J. N. (2004). Twinkle and POLG defects enhance age-dependent accumulation of mutations in the control region of mtDNA. Nucleic Acids Res 32: 3053-3064 [Abstract] [Full Text]  
• Sasaki, N., Kuroiwa, H., Nishitani, C., Takano, H., Higashiyama, T., Kobayashi, T., Shirai, Y., Sakai, A., Kawano, S., Murakami-Murofushi, K., Kuroiwa, T. (2003). Glom Is a Novel Mitochondrial DNA Packaging Protein in Physarum polycephalum and Causes Intense Chromatin Condensation without Suppressing DNA Functions. Mol. Biol. Cell 14: 4758-4769 [Abstract] [Full Text]  
• Garstka, H. L., Schmitt, W. E., Schultz, J., Sogl, B., Silakowski, B., Perez-Martos, A., Montoya, J., Wiesner, R. J. (2003). Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA. Nucleic Acids Res 31: 5039-5047 [Abstract] [Full Text]  
• Mambo, E., Gao, X., Cohen, Y., Guo, Z., Talalay, P., Sidransky, D. (2003). Electrophile and oxidant damage of mitochondrial DNA leading to rapid evolution of homoplasmic mutations. Proc. Natl. Acad. Sci. USA 100: 1838-1843 [Abstract] [Full Text]  
• Eads, B. D., Hand, S. C. (2003). Transcriptional initiation under conditions of anoxia-induced quiescence in mitochondria from Artemia franciscana embryos. J. Exp. Biol. 206: 577-589 [Abstract] [Full Text]  
• Hiendleder, S., Zakhartchenko, V., Wenigerkind, H., Reichenbach, H.-D., Bruggerhoff, K., Prelle, K., Brem, G., Stojkovic, M., Wolf, E. (2003). Heteroplasmy in Bovine Fetuses Produced by Intra- and Inter-Subspecific Somatic Cell Nuclear Transfer: Neutral Segregation of Nuclear Donor Mitochondrial DNA in Various Tissues and Evidence for Recipient Cow Mitochondria in Fetal Blood. Biol. Reprod. 68: 159-166 [Abstract] [Full Text]  
• Bruggerhoff, K., Zakhartchenko, V., Wenigerkind, H., Reichenbach, H.-D., Prelle, K., Schernthaner, W., Alberio, R., Kuchenhoff, H., Stojkovic, M., Brem, G., Hiendleder, S., Wolf, E. (2002). Bovine Somatic Cell Nuclear Transfer Using Recipient Oocytes Recovered by Ovum Pick-Up: Effect of Maternal Lineage of Oocyte Donors. Biol. Reprod. 66: 367-373 [Abstract] [Full Text]  
• Gensler, S., Weber, K., Schmitt, W. E., Perez-Martos, A., Enriquez, J. A., Montoya, J., Wiesner, R. J. (2001). Mechanism of mammalian mitochondrial DNA replication: import of mitochondrial transcription factor A into isolated mitochondria stimulates 7S DNA synthesis. Nucleic Acids Res 29: 3657-3663 [Abstract] [Full Text]  
• Matson, C. W., Baker, R. J. (2001). DNA Sequence Variation in the Mitochondrial Control Region of Red-Backed Voles (Clethrionomys). Mol Biol Evol 18: 1494-1501 [Abstract] [Full Text]  
• Wang, Y., Michikawa, Y., Mallidis, C., Bai, Y., Woodhouse, L., Yarasheski, K. E., Miller, C. A., Askanas, V., Engel, W. K., Bhasin, S., Attardi, G. (2001). Muscle-specific mutations accumulate with aging in critical human mtDNA control sites for replication. Proc. Natl. Acad. Sci. USA 10.1073/pnas.061013598v1 [Abstract] [Full Text]  
• Michikawa, Y., Mazzucchelli, F., Bresolin, N., Scarlato, G., Attardi, G. (1999). Aging-Dependent Large Accumulation of Point Mutations in the Human mtDNA Control Region for Replication. Science 286: 774-779 [Abstract] [Full Text]  
• Enriquez, J. A., Fernandez-Silva, P., Garrido-Perez, N., Lopez-Perez, M. J., Perez-Martos, A., Montoya, J. (1999). Direct Regulation of Mitochondrial RNA Synthesis by Thyroid Hormone. Mol. Cell. Biol. 19: 657-670 [Abstract] [Full Text]  
• Micol, V., Fernandez-Silva, P., Attardi, G. (1997). Functional Analysis of in Vivo and in Organello Footprinting of HeLa Cell Mitochondrial DNA in Relationship to ATP and Ethidium Bromide Effects on Transcription. J. Biol. Chem. 272: 18896-18904 [Abstract] [Full Text]  
• Cantatore, P., Daddabbo, L., Fracasso, F., Gadaleta, M. N. (1995). Identification by in Organello Footprinting of Protein Contact Sites and of Single-stranded DNA Sequences in the Regulatory Region of Rat Mitochondrial DNA. J. Biol. Chem. 270: 25020-25027 [Abstract] [Full Text]  
• Wang, Y., Michikawa, Y., Mallidis, C., Bai, Y., Woodhouse, L., Yarasheski, K. E., Miller, C. A., Askanas, V., Engel, W. K., Bhasin, S., Attardi, G. (2001). Muscle-specific mutations accumulate with aging in critical human mtDNA control sites for replication. Proc. Natl. Acad. Sci. USA 98: 4022-4027 [Abstract] [Full Text]