This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Iida, H Articles by Anraku, Y Search for Related Content PubMed PubMed Citation Articles by Iida, H Articles by Anraku, Y

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 December; 14(12): 8259-8271

MID1, a novel Saccharomyces cerevisiae gene encoding a plasma membrane protein, is required for Ca2+ influx and mating.

Division of Cell Proliferation, National Institute for Basic Biology, Okazakii, Japan.

ABSTRACT

By establishing a unique screening method, we have isolated yeast mutants that die only after differentiating into cells with a mating projection, and some of them are also defective in Ca2+ signaling. The mutants were classified into five complementation groups, one of which we studied extensively. This mutation defines a new gene, designated MID1, which encodes an N-glycosylated, integral plasma membrane protein with 548 amino acid residues. The mid1-1 mutant has low Ca2+ uptake activity, loses viability after receiving mating pheromones, and escapes death when incubated with high concentrations of CaCl2. The MID1 gene is nonessential for vegetative growth. The efficiency of mating between MATa mid1-1 and MAT alpha mid1-1 cells is low. These results demonstrate that MID1 is required for Ca2+ influx and mating.

This article has been cited by other articles:

• Liu, M., Gelli, A. (2008). Elongation Factor 3, EF3, Associates with the Calcium Channel Cch1 and Targets Cch1 to the Plasma Membrane in Cryptococcus neoformans. Eukaryot Cell 7: 1118-1126 [Abstract] [Full Text]  
• Lew, R. R., Abbas, Z., Anderca, M. I., Free, S. J. (2008). Phenotype of a Mechanosensitive Channel Mutant, mid-1, in a Filamentous Fungus, Neurospora crassa. Eukaryot Cell 7: 647-655 [Abstract] [Full Text]  
• Hallen, H. E., Trail, F. (2008). The L-Type Calcium Ion Channel Cch1 Affects Ascospore Discharge and Mycelial Growth in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum). Eukaryot Cell 7: 415-424 [Abstract] [Full Text]  
• Iida, K., Teng, J., Tada, T., Saka, A., Tamai, M., Izumi-Nakaseko, H., Adachi-Akahane, S., Iida, H. (2007). Essential, Completely Conserved Glycine Residue in the Domain III S2 S3 Linker of Voltage-gated Calcium Channel {alpha}1 Subunits in Yeast and Mammals. J. Biol. Chem. 282: 25659-25667 [Abstract] [Full Text]  
• Liu, M., Du, P., Heinrich, G., Cox, G. M., Gelli, A. (2006). Cch1 Mediates Calcium Entry in Cryptococcus neoformans and Is Essential in Low-Calcium Environments. Eukaryot Cell 5: 1788-1796 [Abstract] [Full Text]  
• Zhang, N.-N., Dudgeon, D. D., Paliwal, S., Levchenko, A., Grote, E., Cunningham, K. W. (2006). Multiple Signaling Pathways Regulate Yeast Cell Death during the Response to Mating Pheromones. Mol. Biol. Cell 17: 3409-3422 [Abstract] [Full Text]  
• Noma, S., Iida, K., Iida, H. (2005). Polarized Morphogenesis Regulator Spa2 Is Required for the Function of Putative Stretch-Activated Ca2+-Permeable Channel Component Mid1 in Saccharomyces cerevisiae. Eukaryot Cell 4: 1353-1363 [Abstract] [Full Text]  
• Levin, D. E. (2005). Cell Wall Integrity Signaling in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 69: 262-291 [Abstract] [Full Text]  
• Cortes, J. C. G., Katoh-Fukui, R., Moto, K., Ribas, J. C., Ishiguro, J. (2004). Schizosaccharomyces pombe Pmr1p Is Essential for Cell Wall Integrity and Is Required for Polarized Cell Growth and Cytokinesis. Eukaryot Cell 3: 1124-1135 [Abstract] [Full Text]  
• Ton, V.-K., Rao, R. (2004). Functional expression of heterologous proteins in yeast: insights into Ca2+ signaling and Ca2+-transporting ATPases. Am. J. Physiol. Cell Physiol. 287: C580-C589 [Abstract] [Full Text]  
• Kurusu, T., Sakurai, Y., Miyao, A., Hirochika, H., Kuchitsu, K. (2004). Identification of a Putative Voltage-Gated Ca2+-permeable Channel (OsTPC1) Involved in Ca2+ Influx and Regulation of Growth and Development in Rice. Plant Cell Physiol 45: 693-702 [Abstract] [Full Text]  
• Kaur, R., Castano, I., Cormack, B. P. (2004). Functional Genomic Analysis of Fluconazole Susceptibility in the Pathogenic Yeast Candida glabrata: Roles of Calcium Signaling and Mitochondria. Antimicrob. Agents Chemother. 48: 1600-1613 [Abstract] [Full Text]  
• Hashimoto, K., Saito, M., Matsuoka, H., Iida, K., Iida, H. (2004). Functional Analysis of a Rice Putative Voltage-Dependent Ca2+ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1. Plant Cell Physiol 45: 496-500 [Abstract] [Full Text]  
• Mackin, N. A., Sousou, T. J., Erdman, S. E. (2004). The PXL1 Gene of Saccharomyces cerevisiae Encodes a Paxillin-like Protein Functioning in Polarized Cell Growth. Mol. Biol. Cell 15: 1904-1917 [Abstract] [Full Text]  
• Hamasaki-Katagiri, N., Molchanova, T., Takeda, K., Ames, J. B. (2004). Fission Yeast Homolog of Neuronal Calcium Sensor-1 (Ncs1p) Regulates Sporulation and Confers Calcium Tolerance. J. Biol. Chem. 279: 12744-12754 [Abstract] [Full Text]  
• Suzuki, M., Hirao, A., Mizuno, A. (2003). Microfilament-associated Protein 7 Increases the Membrane Expression of Transient Receptor Potential Vanilloid 4 (TRPV4). J. Biol. Chem. 278: 51448-51453 [Abstract] [Full Text]  
• Muller, E. M., Mackin, N. A., Erdman, S. E., Cunningham, K. W. (2003). Fig1p Facilitates Ca2+ Influx and Cell Fusion during Mating of Saccharomyces cerevisiae. J. Biol. Chem. 278: 38461-38469 [Abstract] [Full Text]  
• Bonilla, M., Cunningham, K. W. (2003). Mitogen-activated Protein Kinase Stimulation of Ca2+ Signaling Is Required for Survival of Endoplasmic Reticulum Stress in Yeast. Mol. Biol. Cell 14: 4296-4305 [Abstract] [Full Text]  
• Tada, T., Ohmori, M., Iida, H. (2003). Molecular Dissection of the Hydrophobic Segments H3 and H4 of the Yeast Ca2+ Channel Component Mid1. J. Biol. Chem. 278: 9647-9654 [Abstract] [Full Text]  
• Zhang, M., Bennett, D., Erdman, S. E. (2002). Maintenance of Mating Cell Integrity Requires the Adhesin Fig2p. Eukaryot Cell 1: 811-822 [Abstract] [Full Text]  
• Matsumoto, T. K., Ellsmore, A. J., Cessna, S. G., Low, P. S., Pardo, J. M., Bressan, R. A., Hasegawa, P. M. (2002). An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae. J. Biol. Chem. 277: 33075-33080 [Abstract] [Full Text]  
• Monteoliva, L., Lopez Matas, M., Gil, C., Nombela, C., Pla, J. (2002). Large-Scale Identification of Putative Exported Proteins in Candida albicans by Genetic Selection. Eukaryot Cell 1: 514-525 [Abstract] [Full Text]  
• Hohmann, S. (2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev. 66: 300-372 [Abstract] [Full Text]  
• Bonilla, M., Cunningham, K. W. (2002). Calcium Release and Influx in Yeast: TRPC and VGCC Rule Another Kingdom. Sci Signal 2002: pe17-pe17 [Abstract] [Full Text]  
• Gage, M. J., Rane, S. G., Hockerman, G. H., Smith, T. J. (2002). The Virally Encoded Fungal Toxin KP4 Specifically Blocks L-Type Voltage-Gated Calcium Channels. Mol. Pharmacol. 61: 936-944 [Abstract] [Full Text]  
• Maruoka, T., Nagasoe, Y., Inoue, S., Mori, Y., Goto, J., Ikeda, M., Iida, H. (2002). Essential Hydrophilic Carboxyl-terminal Regions Including Cysteine Residues of the Yeast Stretch-activated Calcium-permeable Channel Mid1. J. Biol. Chem. 277: 11645-11652 [Abstract] [Full Text]  
• de Bettignies, G., Thoraval, D., Morel, C., Peypouquet, M. F., Crouzet, M. (2001). Overactivation of the Protein Kinase C-Signaling Pathway Suppresses the Defects of Cells Lacking the Rho3/Rho4-GAP Rgd1p in Saccharomyces cerevisiae. Genetics 159: 1435-1448 [Abstract] [Full Text]  
• Muller, E. M., Locke, E. G., Cunningham, K. W. (2001). Differential Regulation of Two Ca2+ Influx Systems by Pheromone Signaling in Saccharomyces cerevisiae. Genetics 159: 1527-1538 [Abstract] [Full Text]  
• Furuichi, T., Cunningham, K. W., Muto, S. (2001). A Putative Two Pore Channel AtTPC1 Mediates Ca2+ Flux in Arabidopsis Leaf Cells. Plant Cell Physiol 42: 900-905 [Abstract] [Full Text]  
• Hamill, O. P., Martinac, B. (2001). Molecular Basis of Mechanotransduction in Living Cells. Physiol. Rev. 81: 685-740 [Abstract] [Full Text]  
• Philip, B., Levin, D. E. (2001). Wsc1 and Mid2 Are Cell Surface Sensors for Cell Wall Integrity Signaling That Act through Rom2, a Guanine Nucleotide Exchange Factor for Rho1. Mol. Cell. Biol. 21: 271-280 [Abstract] [Full Text]  
• Abe, F., Horikoshi, K. (2000). Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 8093-8102 [Abstract] [Full Text]  
• Marcoux, N., Cloutier, S., Zakrzewska, E., Charest, P.-M., Bourbonnais, Y., Pallotta, D. (2000). Suppression of the Profilin-Deficient Phenotype by the RHO2 Signaling Pathway in Saccharomyces cerevisiae. Genetics 156: 579-592 [Abstract] [Full Text]  
• Locke, E. G., Bonilla, M., Liang, L., Takita, Y., Cunningham, K. W. (2000). A Homolog of Voltage-Gated Ca2+ Channels Stimulated by Depletion of Secretory Ca2+ in Yeast. Mol. Cell. Biol. 20: 6686-6694 [Abstract] [Full Text]  
• Qiu, H., Hu, C., Anderson, J., Björk, G. R., Sarkar, S., Hopper, A. K., Hinnebusch, A. G. (2000). Defects in tRNA Processing and Nuclear Export Induce GCN4 Translation Independently of Phosphorylation of the alpha Subunit of Eukaryotic Translation Initiation Factor 2. Mol. Cell. Biol. 20: 2505-2516 [Abstract] [Full Text]  
• Kanzaki, M., Nagasawa, M., Kojima, I., Sato, C., Naruse, K., Sokabe, M., Iida, H. (1999). Molecular Identification of a Eukaryotic, Stretch-Activated Nonselective Cation Channel. Science 285: 882-886 [Abstract] [Full Text]  
• Rajavel, M., Philip, B., Buehrer, B. M., Errede, B., Levin, D. E. (1999). Mid2 Is a Putative Sensor for Cell Integrity Signaling in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 3969-3976 [Abstract] [Full Text]  
• Gustin, M. C., Albertyn, J., Alexander, M., Davenport, K. (1998). MAP Kinase Pathways in the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1264-1300 [Abstract] [Full Text]  
• Clemens, S., Antosiewicz, D. M., Ward, J. M., Schachtman, D. P., Schroeder, J. I. (1998). The plant cDNA LCT1 mediates the uptake of calcium and cadmium in yeast. Proc. Natl. Acad. Sci. USA 95: 12043-12048 [Abstract] [Full Text]  
• Erdman, S., Lin, L., Malczynski, M., Snyder, M. (1998). Pheromone-regulated Genes Required for Yeast Mating Differentiation. J. Cell Biol. 140: 461-483 [Abstract] [Full Text]  
• Matheos, D. P., Kingsbury, T. J., Ahsan, U. S., Cunningham, K. W. (1997). Tcn1p/Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae. Genes Dev. 11: 3445-3458 [Abstract] [Full Text]  
• Nagasawa, M., Kanzaki, M., Iino, Y., Morishita, Y., Kojima, I. (2001). Identification of a Novel Chloride Channel Expressed in the Endoplasmic Reticulum, Golgi Apparatus, and Nucleus. J. Biol. Chem. 276: 20413-20418 [Abstract] [Full Text]  
• Denis, V., Cyert, M. S. (2002). Internal Ca2+ release in yeast is triggered by hypertonic shock and mediated by a TRP channel homologue. J. Cell Biol. 156: 29-34 [Abstract] [Full Text]