This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Skerjanc, I S
Right arrow Articles by McBurney, M W
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Skerjanc, I S
Right arrow Articles by McBurney, M W

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 December; 14(12): 8451-8459

Cellular aggregation enhances MyoD-directed skeletal myogenesis in embryonal carcinoma cells.

I S Skerjanc, R S Slack and M W McBurney

Department of Medicine, University of Ottawa, Ontario, Canada.

ABSTRACT

When introduced into P19 embryonal carcinoma cells, recombinant genes encoding MyoD converted only a small percentage (< 3%) of the transfected cells into skeletal muscle. We isolated stably transfected cells that expressed the MyoD transcript. These P19[MyoD] cells continued to express markers characteristic of undifferentiated stem cells but also expressed myf-5 and the myotonic dystrophy kinase, transcripts normally present in myoblasts but absent from P19 cells. Aggregation of P19[MyoD] cells induced the expression of myogenin, desmin, and the retinoblastoma protein and resulted in the rapid and abundant development of skeletal muscle. Both the embryonic and the slow isoforms of myosin heavy chain were present in this muscle, indicating that it resembled skeletal muscle formed from primary myoblasts. Since aggregation of P19 cells normally results in inefficient differentiation and the development of only low levels of cardiac muscle but no skeletal muscle, we conclude that MyoD imposes the skeletal muscle program on P19 cells and that the differentiation of these cells requires inductive events provided by cell aggregation.

Mol Cell Biol. 1994 December; 14(12): 8451-8459




This article has been cited by other articles:

  • Endo, M., Antonyak, M. A., Cerione, R. A. (2009). Cdc42-mTOR Signaling Pathway Controls Hes5 and Pax6 Expression in Retinoic Acid-dependent Neural Differentiation. J. Biol. Chem. 284: 5107-5118 [Abstract] [Full Text]  
  • Pan, W., Jia, Y., Wang, J., Tao, D., Gan, X., Tsiokas, L., Jing, N., Wu, D., Li, L. (2005). {beta}-Catenin regulates myogenesis by relieving I-mfa-mediated suppression of myogenic regulatory factors in P19 cells. Proc. Natl. Acad. Sci. USA 102: 17378-17383 [Abstract] [Full Text]  
  • Yoshiko, Y., Hirao, K., Maeda, N. (2002). Differentiation in C2C12 myoblasts depends on the expression of endogenous IGFs and not serum depletion. Am. J. Physiol. Cell Physiol. 283: C1278-C1286 [Abstract] [Full Text]  
  • Velling, T., Risteli, J., Wennerberg, K., Mosher, D. F., Johansson, S. (2002). Polymerization of Type I and III Collagens Is Dependent On Fibronectin and Enhanced By Integrins alpha 11beta 1 and alpha 2beta 1. J. Biol. Chem. 277: 37377-37381 [Abstract] [Full Text]  
  • Turner, S. D., Ricci, A. R., Petropoulos, H., Genereaux, J., Skerjanc, I. S., Brandl, C. J. (2002). The E2 Ubiquitin Conjugase Rad6 Is Required for the ArgR/Mcm1 Repression of ARG1 Transcription. Mol. Cell. Biol. 22: 4011-4019 [Abstract] [Full Text]  
  • Petropoulos, H., Skerjanc, I. S. (2002). beta -Catenin Is Essential and Sufficient for Skeletal Myogenesis in P19 Cells. J. Biol. Chem. 277: 15393-15399 [Abstract] [Full Text]  
  • Toyofuku, T., Hong, Z., Kuzuya, T., Tada, M., Hori, M. (2000). WNT/Frizzled-2 Signaling Induces Aggregation and Adhesion among Cardiac Myocytes by Increased Cadherin-{beta}-Catenin Complex. JCB 150: 225-242 [Abstract] [Full Text]  
  • Cozzolino, M, Giovannone, B, Serafino, A, Knudsen, K, Levi, A, Alema, S, Salvatore, A (2000). Activation of TrkA tyrosine kinase in embryonal carcinoma cells promotes cell compaction, independently of tyrosine phosphorylation of catenins. J. Cell Sci. 113: 1601-1610 [Abstract]  
  • Storbeck, C. J., Sabourin, L. A., Waring, J. D., Korneluk, R. G. (1998). Definition of Regulatory Sequence Elements in the Promoter Region and the First Intron of the Myotonic Dystrophy Protein Kinase Gene. J. Biol. Chem. 273: 9139-9147 [Abstract] [Full Text]  
  • Sabourin, L. A., Tamai, K., Narang, M. A., Korneluk, R. G. (1997). Overexpression of 3'-Untranslated Region of the Myotonic Dystrophy Kinase cDNA Inhibits Myoblast Differentiation in Vitro. J. Biol. Chem. 272: 29626-29635 [Abstract] [Full Text]  
  • Redfield, A., Nieman, M. T., Knudsen, K. A. (1997). Cadherins Promote Skeletal Muscle Differentiation in Three-dimensional Cultures. JCB 138: 1323-1331 [Abstract] [Full Text]  
  • Grepin, C, Nemer, G, Nemer, M (1997). Enhanced cardiogenesis in embryonic stem cells overexpressing the GATA-4 transcription factor. Development 124: 2387-2395 [Abstract]  
  • Skerjanc, I. S., Truong, J., Filion, P., McBurney, M. W. (1996). A Splice Variant of the ITF-2 Transcript Encodes a Transcription Factor That Inhibits MyoD Activity. J. Biol. Chem. 271: 3555-3561 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»