This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, C Y
Right arrow Articles by Leiden, J M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, C Y
Right arrow Articles by Leiden, J M

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 February; 14(2): 1153-1159

Activation of the granulocyte-macrophage colony-stimulating factor promoter in T cells requires cooperative binding of Elf-1 and AP-1 transcription factors.

C Y Wang, A G Bassuk, L H Boise, C B Thompson, R Bravo and J M Leiden

Department of Medicine, University of Chicago, Illinois 60637.

ABSTRACT

The granulocyte-macrophage colony-stimulating factor (GM-CSF) gene has been studied extensively as a model system of transcriptional induction during T-lymphocyte activation. The GM-CSF gene is not expressed in resting peripheral blood T cells but is rapidly induced at the transcriptional level following activation through the cell surface T-cell receptor. A highly conserved 19-bp element located immediately 5' of the human GM-CSF TATA box (bp -34 to -52), herein called purine box 1 (PB1), has been shown to bind a T-cell nuclear protein complex and to be required for transcriptional induction of the GM-CSF gene following T-cell activation. The PB1 sequence motif is highly conserved in both human and murine GM-CSF genes. In this report, we demonstrate that the PB1 element alone confers inducibility on a heterologous promoter following transfection into human Jurkat T cells. In addition, we identify a major PB1 nuclear protein-binding complex that is not present in resting peripheral blood T cells but is rapidly induced following T-cell activation. Sequence analysis revealed that PB1 is composed of adjacent binding sites for Ets and AP-1 transcription factors. In vitro mutagenesis experiments demonstrated that both the Ets and AP-1 sites are required for binding of the inducible PB1 nuclear protein complex and for the transcriptional activity of this element and the GM-CSF promoter in activated T cells. Using antibodies specific for different Ets and AP-1 family members, we demonstrate that the major inducible PB1-binding activity present in activated T-cell nuclear extracts is composed of the Elf-1, c-Fos, and JunB transcription factors. Taken together, these results suggest that cooperative interactions between specific Ets and AP-1 family members are important in regulating inducible gene expression following T-cell activation.

Mol Cell Biol. 1994 February; 14(2): 1153-1159




This article has been cited by other articles:

  • Wei, P., Taniguchi, S., Sakai, Y., Imamura, M., Inoguchi, T., Nawata, H., Oda, S., Nakabeppu, Y., Nishimura, J., Ikuyama, S. (2005). Expression of Adipose Differentiation-Related Protein (ADRP) Is Conjointly Regulated by PU.1 and AP-1 in Macrophages. J Biochem 138: 399-412 [Abstract] [Full Text]  
  • Mishra, J. P., Mishra, S., Gee, K., Kumar, A. (2005). Differential Involvement of Calmodulin-dependent Protein Kinase II-activated AP-1 and c-Jun N-terminal Kinase-activated EGR-1 Signaling Pathways in Tumor Necrosis Factor-{alpha} and Lipopolysaccharide-induced CD44 Expression in Human Monocytic Cells. J. Biol. Chem. 280: 26825-26837 [Abstract] [Full Text]  
  • Ma, W., Gee, K., Lim, W., Chambers, K., Angel, J. B., Kozlowski, M., Kumar, A. (2004). Dexamethasone Inhibits IL-12p40 Production in Lipopolysaccharide-Stimulated Human Monocytic Cells by Down-Regulating the Activity of c-Jun N-Terminal Kinase, the Activation Protein-1, and NF-{kappa}B Transcription Factors. J. Immunol. 172: 318-330 [Abstract] [Full Text]  
  • O'Reilly, D., Quinn, C. M., El-Shanawany, T., Gordon, S., Greaves, D. R. (2003). Multiple Ets Factors and Interferon Regulatory Factor-4 Modulate CD68 Expression in a Cell Type-specific Manner. J. Biol. Chem. 278: 21909-21919 [Abstract] [Full Text]  
  • Juang, Y.-T., Tenbrock, K., Nambiar, M. P., Gourley, M. F., Tsokos, G. C. (2002). Defective Production of Functional 98-kDa Form of Elf-1 Is Responsible for the Decreased Expression of TCR {zeta}-Chain in Patients with Systemic Lupus Erythematosus. J. Immunol. 169: 6048-6055 [Abstract] [Full Text]  
  • Tsytsykova, A. V., Goldfeld, A. E. (2002). Inducer-Specific Enhanceosome Formation Controls Tumor Necrosis Factor Alpha Gene Expression in T Lymphocytes. Mol. Cell. Biol. 22: 2620-2631 [Abstract] [Full Text]  
  • Juang, Y.-T., Solomou, E. E., Rellahan, B., Tsokos, G. C. (2002). Phosphorylation and O-Linked Glycosylation of Elf-1 Leads to Its Translocation to the Nucleus and Binding to the Promoter of the TCR {zeta}-Chain. J. Immunol. 168: 2865-2871 [Abstract] [Full Text]  
  • Li, S.-L., Valente, A. J., Wang, L., Gamez, M. J., Clark, R. A. (2001). Transcriptional Regulation of the p67phox Gene. ROLE OF AP-1 IN CONCERT WITH MYELOID-SPECIFIC TRANSCRIPTION FACTORS. J. Biol. Chem. 276: 39368-39378 [Abstract] [Full Text]  
  • Smith, P. J., Cousins, D. J., Jee, Y.-K., Staynov, D. Z., Lee, T. H., Lavender, P. (2001). Suppression of Granulocyte-Macrophage Colony-Stimulating Factor Expression by Glucocorticoids Involves Inhibition of Enhancer Function by the Glucocorticoid Receptor Binding to Composite NF-AT/Activator Protein-1 Elements. J. Immunol. 167: 2502-2510 [Abstract] [Full Text]  
  • Shi, M.-J., Park, S.-R., Kim, P.-H., Stavnezer, J. (2001). Roles of Ets proteins, NF-{{kappa}}B and nocodazole in regulating induction of transcription of mouse germline Ig {{alpha}} RNA by transforming growth factor-{beta}1. Int Immunol 13: 733-746 [Abstract] [Full Text]  
  • Dube, A., Thai, S., Gaspar, J., Rudders, S., Libermann, T. A., Iruela-Arispe, L., Oettgen, P. (2001). ELF-1 Is a Transcriptional Regulator of the Tie2 Gene During Vascular Development. Circ. Res. 88: 237-244 [Abstract] [Full Text]  
  • Biwa, T., Sakai, M., Matsumura, T., Kobori, S., Kaneko, K., Miyazaki, A., Hakamata, H., Horiuchi, S., Shichiri, M. (2000). Sites of Action of Protein Kinase C and Phosphatidylinositol 3-Kinase Are Distinct in Oxidized Low Density Lipoprotein-induced Macrophage Proliferation. J. Biol. Chem. 275: 5810-5816 [Abstract] [Full Text]  
  • Greenland, K. J., Jantke, I., Jenatschke, S., Bracken, K. E., Vinson, C., Gellersen, B. (2000). The Human NAD+-Dependent 15-Hydroxyprostaglandin Dehydrogenase Gene Promoter Is Controlled by Ets and Activating Protein-1 Transcription Factors and Progesterone. Endocrinology 141: 581-597 [Abstract] [Full Text]  
  • Matsumura, T., Sakai, M., Matsuda, K., Furukawa, N., Kaneko, K., Shichiri, M. (1999). Cis-acting DNA Elements of Mouse Granulocyte/Macrophage Colony-stimulating Factor Gene Responsive to Oxidized Low Density Lipoprotein. J. Biol. Chem. 274: 37665-37672 [Abstract] [Full Text]  
  • Nishiyama, C., Yokota, T., Okumura, K., Ra1, C. (1999). The Transcription Factors Elf-1 and GATA-1 Bind to Cell-Specific Enhancer Elements of Human High-Affinity IgE Receptor {alpha}-Chain Gene. J. Immunol. 163: 623-630 [Abstract] [Full Text]  
  • Sakai, M., Biwa, T., Matsumura, T., Takemura, T., Matsuda, H., Anami, Y., Sasahara, T., Kobori, S., Shichiri, M. (1999). Glucocorticoid Inhibits Oxidized LDL-Induced Macrophage Growth by Suppressing the Expression of Granulocyte/Macrophage Colony-Stimulating Factor. Arterioscler. Thromb. Vasc. Bio. 19: 1726-1733 [Abstract] [Full Text]  
  • Hoare, S., Copland, J. A., Wood, T. G., Jeng, Y.-J., Izban, M. G., Soloff, M. S. (1999). Identification of a GABP{alpha}/{beta} Binding Site Involved in the Induction of Oxytocin Receptor Gene Expression in Human Breast Cells. Potentiation by c-Fos/c-Jun. Endocrinology 140: 2268-2279 [Abstract] [Full Text]  
  • Blumenthal, S. G., Aichele, G., Wirth, T., Czernilofsky, A. P., Nordheim, A., Dittmer, J. (1999). Regulation of the Human Interleukin-5 Promoter by Ets Transcription Factors. ETS1 AND ETS2, BUT NOT ELF-1, COOPERATE WITH GATA3 AND HTLV-I TAX1. J. Biol. Chem. 274: 12910-12916 [Abstract] [Full Text]  
  • Rusterholz, C., Henrioud, P. C., Nabholz, M. (1999). Interleukin-2 (IL-2) Regulates the Accessibility of the IL-2-Responsive Enhancer in the IL-2 Receptor alpha  Gene to Transcription Factors. Mol. Cell. Biol. 19: 2681-2689 [Abstract] [Full Text]  
  • Hettmann, T., DiDonato, J., Karin, M., Leiden, J. M. (1999). An Essential Role for Nuclear Factor {kappa}B in Promoting Double Positive Thymocyte Apoptosis. JEM 189: 145-158 [Abstract] [Full Text]  
  • Lakshminarayanan, V., Drab-Weiss, E. A., Roebuck, K. A. (1998). H2O2 and Tumor Necrosis Factor-alpha Induce Differential Binding of the Redox-responsive Transcription Factors AP-1 and NF-kappa B to the Interleukin-8 Promoter in Endothelial and Epithelial Cells. J. Biol. Chem. 273: 32670-32678 [Abstract] [Full Text]  
  • Bockamp, E.-O., Fordham, J. L., Gottgens, B., Murrell, A. M., Sanchez, M.-J., Green, A. R. (1998). Transcriptional Regulation of the Stem Cell Leukemia Gene by PU.1 and Elf-1. J. Biol. Chem. 273: 29032-29042 [Abstract] [Full Text]  
  • Harris, V. K., Liaudet-Coopman, E. D. E., Boyle, B. J., Wellstein, A., Riegel, A. T. (1998). Phorbol Ester-induced Transcription of a Fibroblast Growth Factor-binding Protein Is Modulated by a Complex Interplay of Positive and Negative Regulatory Promoter Elements. J. Biol. Chem. 273: 19130-19139 [Abstract] [Full Text]  
  • Rellahan, B. L., Jensen, J. P., Howcroft, T. K., Singer, D. S., Bonvini, E., Weissman, A. M. (1998). Elf-1 Regulates Basal Expression from the T Cell Antigen Receptor {zeta}-Chain Gene Promoter. J. Immunol. 160: 2794-2801 [Abstract] [Full Text]  
  • Heydemann, A., Boehmler, J. H., Simon, M. C. (1997). Expression of Two Myeloid Cell-specific Genes Requires the Novel Transcription Factor, c-fes Expression Factor. J. Biol. Chem. 272: 29527-29537 [Abstract] [Full Text]  
  • Serdobova, I., Pla, M., Reichenbach, P., Sperisen, P., Ghysdael, J., Wilson, A., Freeman, J., Nabholz, M. (1997). Elf-1 Contributes to the Function of the Complex Interleukin (IL)-2-responsive Enhancer in the Mouse IL-2 Receptor {alpha} Gene. JEM 185: 1211-1222 [Abstract] [Full Text]  
  • Kitamura, C., Terashita, M. (1997). Expressions of c-jun and jun-B Proto-oncogenes in Odontoblasts during Development of Bovine Tooth Germs. JDR 76: 822-830 [Abstract]  
  • Semenza, G. L., Jiang, B.-H., Leung, S. W., Passantino, R., Concordet, J.-P., Maire, P., Giallongo, A. (1996). Hypoxia Response Elements in the Aldolase A, Enolase 1,and Lactate Dehydrogenase A Gene Promoters Contain Essential Binding Sites for Hypoxia-inducible Factor 1. J. Biol. Chem. 271: 32529-32537 [Abstract] [Full Text]  
  • Akbarali, Y., Oettgen, P., Boltax, J., Libermann, T. A. (1996). ELF-1 Interacts with and Transactivates the IgH Enhancer pi Site. J. Biol. Chem. 271: 26007-26012 [Abstract] [Full Text]  
  • Fu, G. K., Markovitz, D. M. (1996). Purification of the pets Factor. A NUCLEAR PROTEIN THAT BINDS TO THE INDUCIBLE TG-RICH ELEMENT OF THE HUMAN IMMUNODEFICIENCY VIRUS TYPE 2ENHANCER. J. Biol. Chem. 271: 19599-19605 [Abstract] [Full Text]  
  • Taylor, D. S., Laubach, J. P., Nathan, D. G., Mathey-Prevot, B. (1996). Cooperation between Core Binding Factor and Adjacent Promoter Elements Contributes to the Tissue-specific Expression of Interleukin-3. J. Biol. Chem. 271: 14020-14027 [Abstract] [Full Text]  
  • Groupp, E. R., Donovan-Peluso, M. (1996). Lipopolysaccharide Induction of THP-1 Cells Activates Binding of c-Jun, Ets, and Egr-1 to the Tissue Factor Promoter. J. Biol. Chem. 271: 12423-12430 [Abstract] [Full Text]  
  • Siegel, M. D., Zhang, D.-H., Ray, P., Ray, A. (1995). Activation of the Interleukin-5 Promoter by cAMP in Murine EL-4 Cells Requires the GATA-3 and CLE0 Elements. J. Biol. Chem. 270: 24548-24555 [Abstract] [Full Text]  
  • Nakae, K., Nakajima, K., Inazawa, J., Kitaoka, T., Hirano, T. (1995). ERM, a PEA3 Subfamily of Ets Transcription Factors, Can Cooperate with c-Jun. J. Biol. Chem. 270: 23795-23800 [Abstract] [Full Text]  
  • Penco, S., Pastorino, S., Bianchi-Scarr, G., Garrč, C. (1995). Lactoferrin Down-modulates the Activity of the Granulocyte Macrophage Colony-stimulating Factor Promoter in Interleukin-1[IMAGE]-stimulated Cells. J. Biol. Chem. 270: 12263-12268 [Abstract] [Full Text]  
  • Wotton, D., Lake, R. A., Farr, C. J., Owen, M. J. (1995). The High Mobility Group Transcription Factor, SOX4, Transactivates the Human CD2 Enhancer. J. Biol. Chem. 270: 7515-7522 [Abstract] [Full Text]  
  • Fletcher, J., Thummel, C. (1995). The Drosophila E74 gene is required for the proper stage- and tissue-specific transcription of ecdysone-regulated genes at the onset of metamorphosis. Development 121: 1411-1421 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»