This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hsu, H L
Right arrow Articles by Baer, R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hsu, H L
Right arrow Articles by Baer, R

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 February; 14(2): 1256-1265

Preferred sequences for DNA recognition by the TAL1 helix-loop-helix proteins.

H L Hsu, L Huang, J T Tsan, W Funk, W E Wright, J S Hu, R E Kingston and R Baer

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.

ABSTRACT

Tumor-specific activation of the TAL1 gene is the most common genetic alteration seen in patients with T-cell acute lymphoblastic leukemia. The TAL1 gene products contain the basic helix-loop-helix (bHLH) domain, a protein dimerization and DNA-binding motif common to several known transcription factors. A binding-site selection procedure has now been used to evaluate the DNA recognition properties of TAL1. These studies demonstrate that TAL1 polypeptides do not have intrinsic DNA-binding activity, presumably because of their inability to form bHLH homodimers. However, TAL1 readily interacts with any of the known class A bHLH proteins (E12, E47, E2-2, and HEB) to form heterodimers that bind DNA in a sequence-specific manner. The TAL1 heterodimers preferentially recognize a subset of E-box elements (CANNTG) that can be represented by the consensus sequence AACAGATGGT. This consensus is composed of half-sites for recognition by the participating class A bHLH polypeptide (AACAG) and the TAL1 polypeptide (ATGGT). TAL1 heterodimers with DNA-binding activity are readily detected in nuclear extracts of Jurkat, a leukemic cell line derived from a patient with T-cell acute lymphoblastic leukemia. Hence, TAL1 is likely to bind and regulate the transcription of a unique subset of subordinate target genes, some of which may mediate the malignant function of TAL1 during T-cell leukemogenesis.

Mol Cell Biol. 1994 February; 14(2): 1256-1265




This article has been cited by other articles:

  • Deleuze, V., Chalhoub, E., El-Hajj, R., Dohet, C., Le Clech, M., Couraud, P.-O., Huber, P., Mathieu, D. (2007). TAL-1/SCL and Its Partners E47 and LMO2 Up-Regulate VE-Cadherin Expression in Endothelial Cells. Mol. Cell. Biol. 27: 2687-2697 [Abstract] [Full Text]  
  • Bayele, H. K., McArdle, H., Srai, S. K.S. (2006). Cis and trans regulation of hepcidin expression by upstream stimulatory factor. Blood 108: 4237-4245 [Abstract] [Full Text]  
  • Wen, J., Huang, S., Pack, S. D., Yu, X., Brandt, S. J., Noguchi, C. T. (2005). Tal1/SCL Binding to Pericentromeric DNA Represses Transcription. J. Biol. Chem. 280: 12956-12966 [Abstract] [Full Text]  
  • Kim, S. K., Albrecht, R. A., O'Callaghan, D. J. (2004). A Negative Regulatory Element (Base Pairs -204 to -177) of the EICP0 Promoter of Equine Herpesvirus 1 Abolishes the EICP0 Protein's trans-Activation of Its Own Promoter. J. Virol. 78: 11696-11706 [Abstract] [Full Text]  
  • Ravet, E., Reynaud, D., Titeux, M., Izac, B., Fichelson, S., Romeo, P.-H., Dubart-Kupperschmitt, A., Pflumio, F. (2004). Characterization of DNA-binding-dependent and -independent functions of SCL/TAL1 during human erythropoiesis. Blood 103: 3326-3335 [Abstract] [Full Text]  
  • Lazrak, M., Deleuze, V., Noel, D., Haouzi, D., Chalhoub, E., Dohet, C., Robbins, I., Mathieu, D. (2004). The bHLH TAL-1/SCL regulates endothelial cell migration and morphogenesis. J. Cell Sci. 117: 1161-1171 [Abstract] [Full Text]  
  • Lahlil, R., Lecuyer, E., Herblot, S., Hoang, T. (2004). SCL Assembles a Multifactorial Complex That Determines Glycophorin A Expression. Mol. Cell. Biol. 24: 1439-1452 [Abstract] [Full Text]  
  • Xu, Z., Huang, S., Chang, L.-S., Agulnick, A. D., Brandt, S. J. (2003). Identification of a TAL1 Target Gene Reveals a Positive Role for the LIM Domain-Binding Protein Ldb1 in Erythroid Gene Expression and Differentiation. Mol. Cell. Biol. 23: 7585-7599 [Abstract] [Full Text]  
  • Tremblay, M., Herblot, S., Lecuyer, E., Hoang, T. (2003). Regulation of pTalpha Gene Expression by a Dosage of E2A, HEB, and SCL. J. Biol. Chem. 278: 12680-12687 [Abstract] [Full Text]  
  • Munoz, E., Brewer, M., Baler, R. (2002). Circadian Transcription. THINKING OUTSIDE THE E-BOX. J. Biol. Chem. 277: 36009-36017 [Abstract] [Full Text]  
  • Lecuyer, E., Herblot, S., Saint-Denis, M., Martin, R., Begley, C. G., Porcher, C., Orkin, S. H., Hoang, T. (2002). The SCL complex regulates c-kit expression in hematopoietic cells through functional interaction with Sp1. Blood 100: 2430-2440 [Abstract] [Full Text]  
  • Engel, I., Murre, C. (2002). Disruption of pre-TCR expression accelerates lymphomagenesis in E2A-deficient mice. Proc. Natl. Acad. Sci. USA 99: 11322-11327 [Abstract] [Full Text]  
  • Goardon, N., Schuh, A., Hajar, I., Ma, X., Jouault, H., Dzierzak, E., Romeo, P.-H., Maouche-Chretien, L. (2002). Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation. Blood 100: 491-500 [Abstract] [Full Text]  
  • Zhai, Y., Wu, R., Schwartz, D. R., Darrah, D., Reed, H., Kolligs, F. T., Nieman, M. T., Fearon, E. R., Cho, K. R. (2002). Role of {beta}-Catenin/T-Cell Factor-Regulated Genes in Ovarian Endometrioid Adenocarcinomas. Am. J. Pathol. 160: 1229-1238 [Abstract] [Full Text]  
  • Herblot, S., Aplan, P. D., Hoang, T. (2002). Gradient of E2A Activity in B-Cell Development. Mol. Cell. Biol. 22: 886-900 [Abstract] [Full Text]  
  • Vitelli, L., Condorelli, G., Lulli, V., Hoang, T., Luchetti, L., Croce, C. M., Peschle, C. (2000). A Pentamer Transcriptional Complex Including tal-1 and Retinoblastoma Protein Downmodulates c-kit Expression in Normal Erythroblasts. Mol. Cell. Biol. 20: 5330-5342 [Abstract] [Full Text]  
  • Huang, S., Brandt, S. J. (2000). mSin3A Regulates Murine Erythroleukemia Cell Differentiation through Association with the TAL1 (or SCL) Transcription Factor. Mol. Cell. Biol. 20: 2248-2259 [Abstract] [Full Text]  
  • Courtes, C., Lecointe, N., Le Cam, L., Baudoin, F., Sardet, C., Mathieu-Mahul, D. (2000). Erythroid-specific Inhibition of the tal-1 Intragenic Promoter Is Due to Binding of a Repressor to a Novel Silencer. J. Biol. Chem. 275: 949-958 [Abstract] [Full Text]  
  • Chervinsky, D. S., Zhao, X.-F., Lam, D. H., Ellsworth, M., Gross, K. W., Aplan, P. D. (1999). Disordered T-Cell Development and T-Cell Malignancies in SCL LMO1 Double-Transgenic Mice: Parallels with E2A-Deficient Mice. Mol. Cell. Biol. 19: 5025-5035 [Abstract] [Full Text]  
  • Holmes, M. L., Haley, J. D., Cerruti, L., Zhou, W.-l., Zogos, H., Smith, D. E., Cunningham, J. M., Jane, S. M. (1999). Identification of Id2 as a Globin Regulatory Protein by Representational Difference Analysis of K562 Cells Induced To Express gamma -Globin with a Fungal Compound. Mol. Cell. Biol. 19: 4182-4190 [Abstract] [Full Text]  
  • Begley, C. G., Green, A. R. (1999). The SCL Gene: From Case Report to Critical Hematopoietic Regulator. Blood 93: 2760-2770 [Full Text]  
  • Gregoire, J.-M., Romeo, P.-H. (1999). T-cell Expression of the Human GATA-3 Gene Is Regulated by a Non-lineage-specific Silencer. J. Biol. Chem. 274: 6567-6578 [Abstract] [Full Text]  
  • Engel, I., Murre, C. (1999). Ectopic expression of E47 or E12 promotes the death of E2A-deficient lymphomas. Proc. Natl. Acad. Sci. USA 96: 996-1001 [Abstract] [Full Text]  
  • Zhao, X.-F., Aplan, P. D. (1999). The Hematopoietic Transcription Factor SCL Binds the p44 Subunit of TFIIH. J. Biol. Chem. 274: 1388-1393 [Abstract] [Full Text]  
  • Porcher, C, Liao, E., Fujiwara, Y, Zon, L., Orkin, S. (1999). Specification of hematopoietic and vascular development by the bHLH transcription factor SCL without direct DNA binding. Development 126: 4603-4615 [Abstract]  
  • Ono, Y., Fukuhara, N., Yoshie, O. (1998). TAL1 and LIM-Only Proteins Synergistically Induce Retinaldehyde Dehydrogenase 2 Expression in T-Cell Acute Lymphoblastic Leukemia by Acting as Cofactors for GATA3. Mol. Cell. Biol. 18: 6939-6950 [Abstract] [Full Text]  
  • Rabbitts, T. H. (1998). LMO T-cell translocation oncogenes typify genes activated by chromosomal translocations that alter transcription and developmental processes. Genes Dev. 12: 2651-2657 [Full Text]  
  • Zhang, M.-Y., Harhaj, E. W., Bell, L., Sun, S.-C., Miller, B. A. (1998). Bcl-3 Expression and Nuclear Translocation Are Induced by Granulocyte-Macrophage Colony-Stimulating Factor and Erythropoietin in Proliferating Human Erythroid Precursors. Blood 92: 1225-1234 [Abstract] [Full Text]  
  • Krosl, G., He, G., Lefrancois, M., Charron, F., Romeo, P.-H., Jolicoeur, P., Kirsch, I. R., Nemer, M., Hoang, T. (1998). Transcription Factor SCL Is Required for c-kit Expression and c-Kit Function in Hemopoietic Cells. JEM 188: 439-450 [Abstract] [Full Text]  
  • Barat, C., Rassart, E. (1998). Members of the GATA Family of Transcription Factors Bind to the U3 Region of Cas-Br-E and Graffi Retroviruses and Transactivate Their Expression. J. Virol. 72: 5579-5588 [Abstract] [Full Text]  
  • Anderson, K. P., Crable, S. C., Lingrel, J. B (1998). Multiple Proteins Binding to a GATA-E Box-GATA Motif Regulate the Erythroid Kruppel-like Factor (EKLF) Gene. J. Biol. Chem. 273: 14347-14354 [Abstract] [Full Text]  
  • Deed, R. W., Jasiok, M., Norton, J. D. (1998). Lymphoid-specific Expression of the Id3 Gene in Hematopoietic Cells. SELECTIVE ANTAGONISM OF E2A BASIC HELIX-LOOP-HELIX PROTEIN ASSOCIATED WITH Id3-INDUCED DIFFERENTIATION OF ERYTHROLEUKEMIA CELLS. J. Biol. Chem. 273: 8278-8286 [Abstract] [Full Text]  
  • Park, S. T., Sun, X.-H. (1998). The Tal1 Oncoprotein Inhibits E47-mediated Transcription. MECHANISM OF INHIBITION. J. Biol. Chem. 273: 7030-7037 [Abstract] [Full Text]  
  • Visvader, J. E., Mao, X., Fujiwara, Y., Hahm, K., Orkin, S. H. (1997). The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation. Proc. Natl. Acad. Sci. USA 94: 13707-13712 [Abstract] [Full Text]  
  • Fickett, J. W., Hatzigeorgiou, A. G. (1997). Eukaryotic Promoter Recognition. Genome Res 7: 861-878 [Full Text]  
  • Zhang, M.-Y., Clawson, G. A., Olivieri, N. F., Bell, L. L., Begley, C. G., Miller, B. A. (1997). Expression of SCL Is Normal in Transfusion-Dependent Diamond-Blackfan Anemia But Other bHLH Proteins Are Deficient. Blood 90: 2068-2074 [Abstract] [Full Text]  
  • Langlands, K., Yin, X., Anand, G., Prochownik, E. V. (1997). Differential Interactions of Id Proteins with Basic-Helix-Loop-Helix Transcription Factors. J. Biol. Chem. 272: 19785-19793 [Abstract] [Full Text]  
  • Cooper, C. L., Brady, G., Bilia, F., Iscove, N. N., Quesenberry, P. J. (1997). Expression of the Id Family Helix-Loop-Helix Regulators During Growth and Development in the Hematopoietic System. Blood 89: 3155-3165 [Abstract] [Full Text]  
  • Prasad, K. S.S., Brandt, S. J. (1997). Target-dependent Effect of Phosphorylation on the DNA Binding Activity of the TAL1/SCL Oncoprotein. J. Biol. Chem. 272: 11457-11462 [Abstract] [Full Text]  
  • Ono, Y., Fukuhara, N., Yoshie, O. (1997). Transcriptional Activity of TAL1 in T Cell Acute Lymphoblastic Leukemia (T-ALL) Requires RBTN1 or -2and Induces TALLA1, a Highly Specific Tumor Marker of T-ALL. J. Biol. Chem. 272: 4576-4581 [Abstract] [Full Text]  
  • Elnitski, L., Miller, W., Hardison, R. (1997). Conserved E Boxes Function as Part of the Enhancer in Hypersensitive Site 2of the beta -Globin Locus Control Region. ROLE OF BASIC HELIX-LOOP-HELIX PROTEINS. J. Biol. Chem. 272: 369-378 [Abstract] [Full Text]  
  • Lam, L. T., Bresnick, E. H. (1996). A Novel DNA-binding Protein, HS2NF5, Interacts with a Functionally Important Sequence of the Human beta -Globin Locus Control Region. J. Biol. Chem. 271: 32421-32429 [Abstract] [Full Text]  
  • Nielsen, A. L., Norby, P. L., Pedersen, F. S., Jorgensen, P. (1996). E-box Sequence and Context-dependent TAL1/SCL Modulation of Basic Helix-Loop-Helix Protein-mediated Transcriptional Activation. J. Biol. Chem. 271: 31463-31469 [Abstract] [Full Text]  
  • Orkin, S. H., Orkin, S. H. (1995). Transcription Factors and Hematopoietic Development. J. Biol. Chem. 270: 4955-4958 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»