This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wong, P Articles by Wright, T M Search for Related Content PubMed PubMed Citation Articles by Wong, P Articles by Wright, T M

A unique palindromic element mediates gamma interferon induction of mig gene expression.

Department of Medicine, University of Pittsburgh School of Medicine, Pennsylvania 15261.

ABSTRACT

To define the molecular mechanisms involved in the action of gamma interferon (IFN-gamma), we have analyzed the transcriptional regulation of the mig (monokine induced by gamma interferon) gene, a member of the platelet factor 4-interleukin-8 cytokine family that is expressed in murine macrophages specifically in response to IFN-gamma. Analysis of mig/CAT chimeric constructs transiently transfected into the RAW 264.7 mouse monocytic cell line revealed a unique IFN-gamma-responsive element (gamma RE-1). The sequence of this cis regulatory element defined by deletion analysis contains an imperfect inverted repeat extending 27 bp. Examination of mig/CAT constructs with mutations in gamma RE-1 revealed that the palindromic positions in the element were essential for activity. Consistent with its function as an enhancer, a single copy of gamma RE-1 conferred IFN-gamma inducibility to a heterologous (herpes simplex virus thymidine kinase) promoter. Exonuclease III protection assays demonstrated symmetrical protection of a mig promoter fragment centered about the gamma RE-1 palindromic sequence. Using the gel electrophoretic mobility shift assay, we identified a factor (gamma RF-1) present in nuclear extracts prepared from IFN-gamma-stimulated RAW 264.7 cells which binds to gamma RE-1. The activation of gamma RF-1 occurred rapidly (within 1 min) in response to IFN-gamma and was independent of protein synthesis. Similar to the expression of mig mRNA, the formation of gamma RF-1 was selectively induced by IFN-gamma and not IFN-alpha. The regulation of gene expression through gamma RF-1 and gamma RE-1 may explain the preferential activation of a subset of interferon-inducible genes by IFN-gamma.

This article has been cited by other articles:

• Liang, L., Roizman, B. (2008). Expression of Gamma Interferon-Dependent Genes Is Blocked Independently by Virion Host Shutoff RNase and by US3 Protein Kinase. J. Virol. 82: 4688-4696 [Abstract] [Full Text]  
• Gharavi, N. M., Alva, J. A., Mouillesseaux, K. P., Lai, C., Yeh, M., Yeung, W., Johnson, J., Szeto, W. L., Hong, L., Fishbein, M., Wei, L., Pfeffer, L. M., Berliner, J. A. (2007). Role of the JAK/STAT Pathway in the Regulation of Interleukin-8 Transcription by Oxidized Phospholipids in Vitro and in Atherosclerosis in Vivo. J. Biol. Chem. 282: 31460-31468 [Abstract] [Full Text]  
• Hokeness, K. L., Deweerd, E. S., Munks, M. W., Lewis, C. A., Gladue, R. P., Salazar-Mather, T. P. (2007). CXCR3-Dependent Recruitment of Antigen-Specific T Lymphocytes to the Liver during Murine Cytomegalovirus Infection. J. Virol. 81: 1241-1250 [Abstract] [Full Text]  
• Song, L., Bhattacharya, S., Yunus, A. A., Lima, C. D., Schindler, C. (2006). Stat1 and SUMO modification. Blood 108: 3237-3244 [Abstract] [Full Text]  
• Medoff, B. D., Wain, J. C., Seung, E., Jackobek, R., Means, T. K., Ginns, L. C., Farber, J. M., Luster, A. D. (2006). CXCR3 and Its Ligands in a Murine Model of Obliterative Bronchiolitis: Regulation and Function.. J. Immunol. 176: 7087-7095 [Abstract] [Full Text]  
• McInnis, K. A., Britain, A., Lausch, R. N., Oakes, J. E. (2005). Synthesis of {alpha}-Chemokines IP-10, I-TAC, and MIG Are Differentially Regulated in Human Corneal Keratocytes. IOVS 46: 1668-1674 [Abstract] [Full Text]  
• Xi, S., Zhang, Q., Gooding, W. E., Smithgall, T. E., Grandis, J. R. (2003). Constitutive Activation of Stat5b Contributes to Carcinogenesis in Vivo. Cancer Res. 63: 6763-6771 [Abstract] [Full Text]  
• Hou, Z., Srivastava, S., Mistry, M. J., Herbst, M. P., Bailey, J. P., Horseman, N. D. (2003). Two Tandemly Linked Interferon-{gamma}-Activated Sequence Elements in the Promoter of Glycosylation-Dependent Cell Adhesion Molecule 1 Gene Synergistically Respond to Prolactin in Mouse Mammary Epithelial Cells. Mol. Endocrinol. 17: 1910-1920 [Abstract] [Full Text]  
• Pal, R., Sahu, A. (2003). Leptin Signaling in the Hypothalamus during Chronic Central Leptin Infusion. Endocrinology 144: 3789-3798 [Abstract] [Full Text]  
• Xi, S., Zhang, Q., Dyer, K. F., Lerner, E. C., Smithgall, T. E., Gooding, W. E., Kamens, J., Grandis, J. R. (2003). Src Kinases Mediate STAT Growth Pathways in Squamous Cell Carcinoma of the Head and Neck. J. Biol. Chem. 278: 31574-31583 [Abstract] [Full Text]  
• Hiroi, M., Ohmori, Y. (2003). The Transcriptional Coactivator CREB-binding Protein Cooperates with STAT1 and NF-kappa B for Synergistic Transcriptional Activation of the CXC Ligand 9/Monokine Induced by Interferon-gamma Gene. J. Biol. Chem. 278: 651-660 [Abstract] [Full Text]  
• Horton, M. R., Boodoo, S., Powell, J. D. (2002). NF-kappa B Activation Mediates the Cross-talk between Extracellular Matrix and Interferon-gamma (IFN-gamma ) Leading to Enhanced Monokine Induced by IFN-gamma (MIG) Expression in Macrophages. J. Biol. Chem. 277: 43757-43762 [Abstract] [Full Text]  
• Kijima, T., Niwa, H., Steinman, R. A., Drenning, S. D., Gooding, W. E., Wentzel, A. L., Xi, S., Grandis, J. R. (2002). STAT3 Activation Abrogates Growth Factor Dependence and Contributes to Head and Neck Squamous Cell Carcinoma Tumor Growth in Vivo. Cell Growth Differ. 13: 355-362 [Abstract] [Full Text]  
• Song, J. I., Lango, M. N., Hwang, J. D., Drenning, S. D., Zeng, Q., Lamph, W. W., Grandis, J. R. (2001). Abrogation of Transforming Growth Factor-{alpha}/Epidermal Growth Factor Receptor Autocrine Signaling by an RXR-selective Retinoid (LGD1069, Targretin) in Head and Neck Cancer Cell Lines. Cancer Res. 61: 5919-5925 [Abstract] [Full Text]  
• Marx, N., Mach, F., Sauty, A., Leung, J. H., Sarafi, M. N., Ransohoff, R. M., Libby, P., Plutzky, J., Luster, A. D. (2000). Peroxisome Proliferator-Activated Receptor-{gamma} Activators Inhibit IFN-{gamma}-Induced Expression of the T Cell-Active CXC Chemokines IP-10, Mig, and I-TAC in Human Endothelial Cells. J. Immunol. 164: 6503-6508 [Abstract] [Full Text]  
• Grandis, J. R., Drenning, S. D., Zeng, Q., Watkins, S. C., Melhem, M. F., Endo, S., Johnson, D. E., Huang, L., He, Y., Kim, J. D. (2000). Constitutive activation of Stat3 signaling abrogates apoptosis in squamous cell carcinogenesis in vivo. Proc. Natl. Acad. Sci. USA 97: 4227-4232 [Abstract] [Full Text]  
• Gasperini, S., Marchi, M., Calzetti, F., Laudanna, C., Vicentini, L., Olsen, H., Murphy, M., Liao, F., Farber, J., Cassatella, M. A. (1999). Gene Expression and Production of the Monokine Induced by IFN-{gamma} (MIG), IFN-Inducible T Cell {alpha} Chemoattractant (I-TAC), and IFN-{gamma}-Inducible Protein-10 (IP-10) Chemokines by Human Neutrophils. J. Immunol. 162: 4928-4937 [Abstract] [Full Text]  
• Horton, M. R., McKee, C. M., Bao, C., Liao, F., Farber, J. M., Hodge-DuFour, J., Pure, E., Oliver, B. L., Wright, T. M., Noble, P. W. (1998). Hyaluronan Fragments Synergize with Interferon-gamma to Induce the C-X-C Chemokines Mig and Interferon-inducible Protein-10 in Mouse Macrophages. J. Biol. Chem. 273: 35088-35094 [Abstract] [Full Text]  
• Ohmori, Y., Hamilton, T. A. (1998). STAT6 Is Required for the Anti-inflammatory Activity of Interleukin-4 in Mouse Peritoneal Macrophages. J. Biol. Chem. 273: 29202-29209 [Abstract] [Full Text]  
• Valente, A. J., Xie, J.-f., Abramova, M. A., Wenzel, U. O., Abboud, H. E., Graves, D. T. (1998). A Complex Element Regulates IFN-{gamma}-Stimulated Monocyte Chemoattractant Protein-1 Gene Transcription. J. Immunol. 161: 3719-3728 [Abstract] [Full Text]  
• Doyle, S. E., Gasson, J. C. (1998). Characterization of the Role of the Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor alpha  Subunit in the Activation of JAK2 and STAT5. Blood 92: 867-876 [Abstract] [Full Text]  
• Ohmori, Y., Schreiber, R. D., Hamilton, T. A. (1997). Synergy between Interferon-gamma and Tumor Necrosis Factor-alpha in Transcriptional Activation Is Mediated by Cooperation between Signal Transducer and Activator of Transcription 1 and Nuclear Factor kappa B. J. Biol. Chem. 272: 14899-14907 [Abstract] [Full Text]  
• Gustafson, K. S., Ginder, G. D. (1996). Interferon-gamma Induction of the Human Leukocyte Antigen-E Gene Is Mediated through Binding of a Complex Containing STAT1alpha to a Distinct Interferon-gamma -responsive Element. J. Biol. Chem. 271: 20035-20046 [Abstract] [Full Text]  
• Bergad, P. L., Shih, H.-M., Towle, H. C., Schwarzenberg, S. J., Berry, S. A. (1995). Growth Hormone Induction of Hepatic Serine Protease Inhibitor 2.1 Transcription Is Mediated by a Stat5-related Factor Binding Synergistically to Two [IMAGE]-Activated Sites. J. Biol. Chem. 270: 24903-24910 [Abstract] [Full Text]  
• Wood, T. J. J., Sliva, D., Lobie, P. E., Pircher, T. J., Gouilleux, F., Wakao, H., Gustafsson, J., Groner, B., Norstedt, G., and Lars-Arne Haldosén, , (1995). Mediation of Growth Hormone-dependent Transcriptional Activation by Mammary Gland Factor/Stat 5. J. Biol. Chem. 270: 9448-9453 [Abstract] [Full Text]  
• Horvath, C M, Wen, Z, Darnell, J E (1995). A STAT protein domain that determines DNA sequence recognition suggests a novel DNA-binding domain.. Genes Dev. 9: 984-994 [Abstract]  
• Ehret, G. B., Reichenbach, P., Schindler, U., Horvath, C. M., Fritz, S., Nabholz, M., Bucher, P. (2001). DNA Binding Specificity of Different STAT Proteins. COMPARISON OF IN VITRO SPECIFICITY WITH NATURAL TARGET SITES. J. Biol. Chem. 276: 6675-6688 [Abstract] [Full Text]  
• Mahalingam, S., Chaudhri, G., Ling Tan, C., John, A., Foster, P. S., Karupiah, G. (2001). Transcription of the Interferon gamma (IFN-gamma )-inducible Chemokine Mig in IFN-gamma -deficient Mice. J. Biol. Chem. 276: 7568-7574 [Abstract] [Full Text]