This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vettese-Dadey, M
Right arrow Articles by Workman, J L
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vettese-Dadey, M
Right arrow Articles by Workman, J L

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 February; 14(2): 970-981

Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores.

M Vettese-Dadey, P Walter, H Chen, L J Juan and J L Workman

Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802.

ABSTRACT

Facilitated, "cooperative" binding of GAL4-AH to nucleosomal DNA occurred in response to inhibition from the core histone amino termini. The binding of GAL4-AH (which contains the DNA-binding and dimerization domains of GAL4) to nucleosome cores containing multiple binding sites initiated at the end of a nucleosome core and proceeded in a cooperative manner until all sites were occupied. However, following tryptic removal of the core histone amino termini, GAL4-AH binding appeared to be noncooperative, similar to binding naked DNA. Binding of GAL4-AH to nucleosomes bearing a single GAL4 site at different positions indicated that inhibition of GAL4 binding was largely mediated by the histone amino termini and primarily occurred at sites well within the core and not near the end. When the histone amino termini were intact, binding of GAL4-AH to sites near the center of a nucleosome core was greatly enhanced by the presence of additional GAL4 dimers bound to more-accessible positions. These data illustrate that the binding of a factor to more-accessible sites, near the end of a nucleosome, allows facilitated binding of additional factors to the center of the nucleosome, thereby overcoming repression from the core histone amino termini. This mechanism may contribute to the binding of multiple factors to complex promoter and enhancer elements in cellular chromatin.

Mol Cell Biol. 1994 February; 14(2): 970-981




This article has been cited by other articles:

  • Ulyanova, N. P., Schnitzler, G. R. (2007). Inverted Factor Access and Slow Reversion Characterize SWI/SNF-altered Nucleosome Dimers. J. Biol. Chem. 282: 1018-1028 [Abstract] [Full Text]  
  • Yang, Z., Zheng, C., Thiriet, C., Hayes, J. J. (2005). The Core Histone N-Terminal Tail Domains Negatively Regulate Binding of Transcription Factor IIIA to a Nucleosome Containing a 5S RNA Gene via a Novel Mechanism. Mol. Cell. Biol. 25: 241-249 [Abstract] [Full Text]  
  • Hernandez-Munain, C., Krangel, M. S. (2002). Distinct Roles for c-Myb and Core Binding Factor/Polyoma Enhancer-Binding Protein 2 in the Assembly and Function of a Multiprotein Complex on the TCR {delta} Enhancer In Vivo. J. Immunol. 169: 4362-4369 [Abstract] [Full Text]  
  • Chen, C., Yang, T. P. (2001). Nucleosomes Are Translationally Positioned on the Active Allele and Rotationally Positioned on the Inactive Allele of the HPRT Promoter. Mol. Cell. Biol. 21: 7682-7695 [Abstract] [Full Text]  
  • Sullivan, E. K., Weirich, C. S., Guyon, J. R., Sif, S., Kingston, R. E. (2001). Transcriptional Activation Domains of Human Heat Shock Factor 1 Recruit Human SWI/SNF. Mol. Cell. Biol. 21: 5826-5837 [Abstract] [Full Text]  
  • Avolio-Hunter, T. M., Lewis, P. N., Frappier, L. (2001). Epstein-Barr nuclear antigen 1 binds and destabilizes nucleosomes at the viral origin of latent DNA replication. Nucleic Acids Res 29: 3520-3528 [Abstract] [Full Text]  
  • Guyon, J. R., Narlikar, G. J., Sullivan, E. K., Kingston, R. E. (2001). Stability of a Human SWI-SNF Remodeled Nucleosomal Array. Mol. Cell. Biol. 21: 1132-1144 [Abstract] [Full Text]  
  • Protacio, R. U., Li, G., Lowary, P. T., Widom, J. (2000). Effects of Histone Tail Domains on the Rate of Transcriptional Elongation through a Nucleosome. Mol. Cell. Biol. 20: 8866-8878 [Abstract] [Full Text]  
  • Smith, C. L., Wolford, R. G., O’Neill, T. B., Hager, G. L. (2000). Characterization of Transiently and Constitutively Expressed Progesterone Receptors: Evidence for Two Functional States. Mol. Endocrinol. 14: 956-971 [Abstract] [Full Text]  
  • Stein, G. S., Montecino, M., van Wijnen, A. J., Stein, J. L., Lian, J. B. (2000). Nuclear Structure-Gene Expression Interrelationships: Implications for Aberrant Gene Expression in Cancer. Cancer Res. 60: 2067-2076 [Abstract] [Full Text]  
  • Vitolo, J. M., Thiriet, C., Hayes, J. J. (2000). The H3-H4 N-Terminal Tail Domains Are the Primary Mediators of Transcription Factor IIIA Access to 5S DNA within a Nucleosome. Mol. Cell. Biol. 20: 2167-2175 [Abstract] [Full Text]  
  • Kingston, R. E., Narlikar, G. J. (1999). ATP-dependent remodeling and acetylation as regulators of chromatin fluidity. Genes Dev. 13: 2339-2352 [Full Text]  
  • Yu, L., Morse, R. H. (1999). Chromatin Opening and Transactivator Potentiation by RAP1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 5279-5288 [Abstract] [Full Text]  
  • Balasubramanian, B., Morse, R. H. (1999). Binding of Gal4p and Bicoid to Nucleosomal Sites in Yeast in the Absence of Replication. Mol. Cell. Biol. 19: 2977-2985 [Abstract] [Full Text]  
  • Guyon, J. R., Narlikar, G. J., Sif, S., Kingston, R. E. (1999). Stable Remodeling of Tailless Nucleosomes by the Human SWI-SNF Complex. Mol. Cell. Biol. 19: 2088-2097 [Abstract] [Full Text]  
  • Motta, M. C., Caretti, G., Badaracco, G. F., Mantovani, R. (1999). Interactions of the CCAAT-binding Trimer NF-Y with Nucleosomes. J. Biol. Chem. 274: 1326-1333 [Abstract] [Full Text]  
  • Mutskov, V., Gerber, D., Angelov, D., Ausio, J., Workman, J., Dimitrov, S. (1998). Persistent Interactions of Core Histone Tails with Nucleosomal DNA following Acetylation and Transcription Factor Binding. Mol. Cell. Biol. 18: 6293-6304 [Abstract] [Full Text]  
  • Thiriet, C., Hayes, J. J. (1998). Functionally Relevant Histone-DNA Interactions Extend Beyond the Classically Defined Nucleosome Core Region. J. Biol. Chem. 273: 21352-21358 [Abstract] [Full Text]  
  • Lefebvre, P., Mouchon, A., Lefebvre, B., Formstecher, P. (1998). Binding of Retinoic Acid Receptor Heterodimers to DNA. A ROLE FOR HISTONES NH2 TERMINI. J. Biol. Chem. 273: 12288-12295 [Abstract] [Full Text]  
  • Ryan, M. P., Jones, R., Morse, R. H. (1998). SWI-SNF Complex Participation in Transcriptional Activation at a Step Subsequent to Activator Binding. Mol. Cell. Biol. 18: 1774-1782 [Abstract] [Full Text]  
  • Xu, M., Simpson, R. T., Kladde, M. P. (1998). Gal4p-Mediated Chromatin Remodeling Depends on Binding Site Position in Nucleosomes but Does Not Require DNA Replication. Mol. Cell. Biol. 18: 1201-1212 [Abstract] [Full Text]  
  • WADE, P.A., JONES, P.L., VERMAAK, D., VEENSTRA, G.J.C., IMHOF, A., SERA, T., TSE, C., GE, H., SHI, Y.-B., HANSEN, J.C., WOLFFE, A.P. (1998). Histone Deacetylase Directs the Dominant Silencing of Transcription in Chromatin: Association with MeCP2 and the Mi-2 Chromodomain SWI/SNF ATPase. Cold Spring Harb Symp Quant Biol 63: 435-446 [Abstract]  
  • STEGER, D.J., UTLEY, R.T., GRANT, P.A., JOHN, S., EBERHARTER, A., COTE, J., OWEN-HUGHES, T., IKEDA, K., WORKMAN, J.L. (1998). Regulation of Transcription by Multisubunit Complexes That Alter Nucleosome Structure. Cold Spring Harb Symp Quant Biol 63: 483-492 [Abstract]  
  • Grant, P A, Duggan, L, Cote, J, Roberts, S M, Brownell, J E, Candau, R, Ohba, R, Owen-Hughes, T, Allis, C D, Winston, F, Berger, S L, Workman, J L (1997). Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex.. Genes Dev. 11: 1640-1650 [Abstract]  
  • Utley, R. T., Cote, J., Owen-Hughes, T., Workman, J. L. (1997). SWI/SNF Stimulates the Formation of Disparate Activator-Nucleosome Complexes but Is Partially Redundant with Cooperative Binding. J. Biol. Chem. 272: 12642-12649 [Abstract] [Full Text]  
  • Juan, L.-J., Utley, R. T., Vignali, M., Bohm, L., Workman, J. L. (1997). H1-mediated Repression of Transcription Factor Binding to a Stably Positioned Nucleosome. J. Biol. Chem. 272: 3635-3640 [Abstract] [Full Text]  
  • Kingston, R E, Bunker, C A, Imbalzano, A N (1996). Repression and activation by multiprotein complexes that alter chromatin structure.. Genes Dev. 10: 905-920 [Abstract]  
  • Ma, X, Yuan, D, Diepold, K, Scarborough, T, Ma, J (1996). The Drosophila morphogenetic protein Bicoid binds DNA cooperatively. Development 122: 1195-1206 [Abstract]  
  • Alevizopoulos, A, Dusserre, Y, Tsai-Pflugfelder, M, von der Weid, T, Wahli, W, Mermod, N (1995). A proline-rich TGF-beta-responsive transcriptional activator interacts with histone H3.. Genes Dev. 9: 3051-3066 [Abstract]  
  • Godde, J. S., Wolffe, A. P. (1995). Disruption of Reconstituted Nucleosomes. J. Biol. Chem. 270: 27399-27402 [Abstract] [Full Text]  
  • Cote, J, Quinn, J, Workman, J., Peterson, C. (1994). Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex. Science 265: 53-60 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»