This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Epstein, C B
Right arrow Articles by Cross, F R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Epstein, C B
Right arrow Articles by Cross, F R

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 March; 14(3): 2041-2047

Genes that can bypass the CLN requirement for Saccharomyces cerevisiae cell cycle START.

C B Epstein and F R Cross

Rockefeller University, New York, New York 10021.

ABSTRACT

Cell cycle START in Saccharomyces cerevisiae requires at least one of the three CLN genes (CLN1, CLN2, or CLN3). A total of 12 mutations bypassing this requirement were found to be dominant mutations in a single gene that we named BYC1 (for bypass of CLN requirement). We also isolated a plasmid that had cln bypass activity at a low copy number; the gene responsible was distinct from BYC1 and was identical to the recently described BCK2 gene. Strains carrying bck2::ARG4 disruption alleles were fully viable, but bck2::ARG4 completely suppressed the cln bypass activity of BYC1. swi4 and swi6 deletion alleles also efficiently suppressed BYC1 cln bypass activity; Swi4 and Swi6 are components of a transcription factor previously implicated in control of CLN1 and CLN2 expression. bck2::ARG4 was synthetically lethal with cln3 deletion, suggesting that CLN1 and CLN2 cannot function in the simultaneous absence of BCK2 and CLN3; this observation correlates with low expression of CLN1 and CLN2 in bck2 strains deprived of CLN3 function. Thus, factors implicated in CLN1 and CLN2 expression and/or function are also required for BYC1 function in the absence of all three CLN genes; this may suggest the involvement of other targets of Swi4, Swi6, and Bck2 in START.

Mol Cell Biol. 1994 March; 14(3): 2041-2047




This article has been cited by other articles:

  • Manukyan, A., Zhang, J., Thippeswamy, U., Yang, J., Zavala, N., Mudannayake, M. P., Asmussen, M., Schneider, C., Schneider, B. L. (2008). Ccr4 Alters Cell Size in Yeast by Modulating the Timing of CLN1 and CLN2 Expression. Genetics 179: 345-357 [Abstract] [Full Text]  
  • Jiang, Y. (2006). Regulation of the Cell Cycle by Protein Phosphatase 2A in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 70: 440-449 [Abstract] [Full Text]  
  • Castillo-Lluva, S., Perez-Martin, J. (2005). The Induction of the Mating Program in the Phytopathogen Ustilago maydis Is Controlled by a G1 Cyclin. Plant Cell 17: 3544-3560 [Abstract] [Full Text]  
  • Bean, J. M., Siggia, E. D., Cross, F. R. (2005). High Functional Overlap Between MluI Cell-Cycle Box Binding Factor and Swi4/6 Cell-Cycle Box Binding Factor in the G1/S Transcriptional Program in Saccharomyces cerevisiae. Genetics 171: 49-61 [Abstract] [Full Text]  
  • Levin, D. E. (2005). Cell Wall Integrity Signaling in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 69: 262-291 [Abstract] [Full Text]  
  • Su, N. Y., Flick, K., Kaiser, P. (2005). The F-Box Protein Met30 Is Required for Multiple Steps in the Budding Yeast Cell Cycle. Mol. Cell. Biol. 25: 3875-3885 [Abstract] [Full Text]  
  • Chapa y Lazo, B., Bates, S., Sudbery, P. (2005). The G1 Cyclin Cln3 Regulates Morphogenesis in Candida albicans. Eukaryot Cell 4: 90-94 [Abstract] [Full Text]  
  • Flick, K., Wittenberg, C. (2005). Multiple Pathways for Suppression of Mutants Affecting G1-Specific Transcription in Saccharomyces cerevisiae. Genetics 169: 37-49 [Abstract] [Full Text]  
  • Schneider, B. L., Zhang, J., Markwardt, J., Tokiwa, G., Volpe, T., Honey, S., Futcher, B. (2004). Growth Rate and Cell Size Modulate the Synthesis of, and Requirement for, G1-Phase Cyclins at Start. Mol. Cell. Biol. 24: 10802-10813 [Abstract] [Full Text]  
  • Chen, K. C., Calzone, L., Csikasz-Nagy, A., Cross, F. R., Novak, B., Tyson, J. J. (2004). Integrative Analysis of Cell Cycle Control in Budding Yeast. Mol. Biol. Cell 15: 3841-3862 [Abstract] [Full Text]  
  • Geymonat, M., Spanos, A., Wells, G. P., Smerdon, S. J., Sedgwick, S. G. (2004). Clb6/Cdc28 and Cdc14 Regulate Phosphorylation Status and Cellular Localization of Swi6. Mol. Cell. Biol. 24: 2277-2285 [Abstract] [Full Text]  
  • Laabs, T. L., Markwardt, D. D., Slattery, M. G., Newcomb, L. L., Stillman, D. J., Heideman, W. (2003). ACE2 is required for daughter cell-specific G1 delay in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 100: 10275-10280 [Abstract] [Full Text]  
  • Costanzo, M., Schub, O., Andrews, B. (2003). G1 Transcription Factors Are Differentially Regulated in Saccharomyces cerevisiae by the Swi6-Binding Protein Stb1. Mol. Cell. Biol. 23: 5064-5077 [Abstract] [Full Text]  
  • Newcomb, L. L., Diderich, J. A., Slattery, M. G., Heideman, W. (2003). Glucose Regulation of Saccharomyces cerevisiae Cell Cycle Genes. Eukaryot Cell 2: 143-149 [Abstract] [Full Text]  
  • Davis, D. A., Bruno, V. M., Loza, L., Filler, S. G., Mitchell, A. P. (2002). Candida albicans Mds3p, a Conserved Regulator of pH Responses and Virulence Identified Through Insertional Mutagenesis. Genetics 162: 1573-1581 [Abstract] [Full Text]  
  • Wijnen, H., Landman, A., Futcher, B. (2002). The G1 Cyclin Cln3 Promotes Cell Cycle Entry via the Transcription Factor Swi6. Mol. Cell. Biol. 22: 4402-4418 [Abstract] [Full Text]  
  • Newcomb, L. L., Hall, D. D., Heideman, W. (2002). AZF1 Is a Glucose-Dependent Positive Regulator of CLN3 Transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 1607-1614 [Abstract] [Full Text]  
  • Sidorova, J. M., Breeden, L. L. (2002). Precocious S-Phase Entry in Budding Yeast Prolongs Replicative State and Increases Dependence Upon Rad53 for Viability. Genetics 160: 123-136 [Abstract] [Full Text]  
  • Cross, F. R., Archambault, V., Miller, M., Klovstad, M. (2002). Testing a Mathematical Model of the Yeast Cell Cycle. Mol. Biol. Cell 13: 52-70 [Abstract] [Full Text]  
  • Miller, M. E., Cross, F. R. (2001). Mechanisms Controlling Subcellular Localization of the G1 Cyclins Cln2p and Cln3p in Budding Yeast. Mol. Cell. Biol. 21: 6292-6311 [Abstract] [Full Text]  
  • Miller, M., Cross, F. (2001). Cyclin specificity: how many wheels do you need on a unicycle?. J. Cell Sci. 114: 1811-1820 [Abstract]  
  • Miller, M. E., Cross, F. R. (2000). Distinct Subcellular Localization Patterns Contribute to Functional Specificity of the Cln2 and Cln3 Cyclins of Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 542-555 [Abstract] [Full Text]  
  • Andrews, P., Stark, M. (2000). Type 1 protein phosphatase is required for maintenance of cell wall integrity, morphogenesis and cell cycle progression in Saccharomyces cerevisiae. J. Cell Sci. 113: 507-520 [Abstract]  
  • Chen, K. C., Csikasz-Nagy, A., Gyorffy, B., Val, J., Novak, B., Tyson, J. J. (2000). Kinetic Analysis of a Molecular Model of the Budding Yeast Cell Cycle. Mol. Biol. Cell 11: 369-391 [Abstract] [Full Text]  
  • Wijnen, H., Futcher, B. (1999). Genetic Analysis of the Shared Role of CLN3 and BCK2 at the G1-S Transition in Saccharomyces cerevisiae. Genetics 153: 1131-1143 [Abstract] [Full Text]  
  • Wu, M., Newcomb, L., Heideman, W. (1999). Regulation of Gene Expression by Glucose in Saccharomyces cerevisiae: a Role for ADA2 and ADA3/NGG1. J. Bacteriol. 181: 4755-4760 [Abstract] [Full Text]  
  • Ho, Y., Costanzo, M., Moore, L., Kobayashi, R., Andrews, B. J. (1999). Regulation of Transcription at the Saccharomyces cerevisiae Start Transition by Stb1, a Swi6-Binding Protein. Mol. Cell. Biol. 19: 5267-5278 [Abstract] [Full Text]  
  • Clotet, J., Gari, E., Aldea, M., Arino, J. (1999). The Yeast Ser/Thr Phosphatases Sit4 and Ppz1 Play Opposite Roles in Regulation of the Cell Cycle. Mol. Cell. Biol. 19: 2408-2415 [Abstract] [Full Text]  
  • Mendenhall, M. D., Hodge, A. E. (1998). Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1191-1243 [Abstract] [Full Text]  
  • Parviz, F., Hall, D. D., Markwardt, D. D., Heideman, W. (1998). Transcriptional Regulation of CLN3 Expression by Glucose in Saccharomyces cerevisiae. J. Bacteriol. 180: 4508-4515 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»