This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Elfring, L K Articles by Tamkun, J W Search for Related Content PubMed PubMed Citation Articles by Elfring, L K Articles by Tamkun, J W

Identification and characterization of Drosophila relatives of the yeast transcriptional activator SNF2/SWI2.

Department of Biology, University of California, Santa Cruz 95064.

ABSTRACT

The Drosophila brahma (brm) gene encodes an activator of homeotic genes that is highly related to the yeast transcriptional activator SWI2 (SNF2), a potential helicase. To determine whether brm is a functional homolog of SWI2 or merely a member of a family of SWI2-related genes, we searched for additional Drosophila genes related to SWI2 and examined their function in yeast cells. In addition to brm, we identified one other Drosophila relative of SWI2: the closely related ISWI gene. The 1,027-residue ISWI protein contains the DNA-dependent ATPase domain characteristic of the SWI2 protein family but lacks the three other domains common to brm and SWI2. In contrast, the ISWI protein is highly related (70% identical) to the human hSNF2L protein over its entire length, suggesting that they may be functional homologs. The DNA-dependent ATPase domains of brm and SWI2, but not ISWI, are functionally interchangeable; a chimeric SWI2-brm protein partially rescued the slow growth of swi2- cells and supported transcriptional activation mediated by the glucocorticoid receptor in vivo in yeast cells. These findings indicate that brm is the closest Drosophila relative of SWI2 and suggest that brm and SWI2 play similar roles in transcriptional activation.

This article has been cited by other articles:

• Trotter, K. W., Fan, H.-Y., Ivey, M. L., Kingston, R. E., Archer, T. K. (2008). The HSA Domain of BRG1 Mediates Critical Interactions Required for Glucocorticoid Receptor-Dependent Transcriptional Activation In Vivo. Mol. Cell. Biol. 28: 1413-1426 [Abstract] [Full Text]  
• Andersen, E. C., Lu, X., Horvitz, H. R. (2006). C. elegans ISWI and NURF301 antagonize an Rb-like pathway in the determination of multiple cell fates. Development 133: 2695-2704 [Abstract] [Full Text]  
• Stockdale, C., Flaus, A., Ferreira, H., Owen-Hughes, T. (2006). Analysis of Nucleosome Repositioning by Yeast ISWI and Chd1 Chromatin Remodeling Complexes. J. Biol. Chem. 281: 16279-16288 [Abstract] [Full Text]  
• Flaus, A., Martin, D. M. A., Barton, G. J., Owen-Hughes, T. (2006). Identification of multiple distinct Snf2 subfamilies with conserved structural motifs. Nucleic Acids Res 34: 2887-2905 [Abstract] [Full Text]  
• Fyodorov, D. V., Blower, M. D., Karpen, G. H., Kadonaga, J. T. (2004). Acf1 confers unique activities to ACF/CHRAC and promotes the formation rather than disruption of chromatin in vivo. Genes Dev. 18: 170-183 [Abstract] [Full Text]  
• Van Doren, M., Mathews, W. R., Samuels, M., Moore, L. A., Broihier, H. T., Lehmann, R. (2003). fear of intimacy encodes a novel transmembrane protein required for gonad morphogenesis in Drosophila. Development 130: 2355-2364 [Abstract] [Full Text]  
• Whitehouse, I., Stockdale, C., Flaus, A., Szczelkun, M. D., Owen-Hughes, T. (2003). Evidence for DNA Translocation by the ISWI Chromatin-Remodeling Enzyme. Mol. Cell. Biol. 23: 1935-1945 [Abstract] [Full Text]  
• Vary, J. C. Jr., Gangaraju, V. K., Qin, J., Landel, C. C., Kooperberg, C., Bartholomew, B., Tsukiyama, T. (2003). Yeast Isw1p Forms Two Separable Complexes In Vivo. Mol. Cell. Biol. 23: 80-91 [Abstract] [Full Text]  
• Fyodorov, D. V., Kadonaga, J. T. (2002). Binding of Acf1 to DNA Involves a WAC Motif and Is Important for ACF-Mediated Chromatin Assembly. Mol. Cell. Biol. 22: 6344-6353 [Abstract] [Full Text]  
• Langst, G., Becker, P. B. (2002). Nucleosome mobilization and positioning by ISWI-containing chromatin-remodeling factors. J. Cell Sci. 114: 2561-2568 [Abstract] [Full Text]  
• Kim, J. K., Huh, S.-O., Choi, H., Lee, K.-S., Shin, D., Lee, C., Nam, J.-S., Kim, H., Chung, H., Lee, H. W., Park, S. D., Seong, R. H. (2001). Srg3, a Mouse Homolog of Yeast SWI3, Is Essential for Early Embryogenesis and Involved in Brain Development. Mol. Cell. Biol. 21: 7787-7795 [Abstract] [Full Text]  
• Clapier, C. R., Längst, G., Corona, D. F. V., Becker, P. B., Nightingale, K. P. (2001). Critical Role for the Histone H4 N Terminus in Nucleosome Remodeling by ISWI. Mol. Cell. Biol. 21: 875-883 [Abstract] [Full Text]  
• Ruhf, M., Braun, A, Papoulas, O, Tamkun, J., Randsholt, N, Meister, M (2001). The domino gene of Drosophila encodes novel members of the SWI2/SNF2 family of DNA-dependent ATPases, which contribute to the silencing of homeotic genes. Development 128: 1429-1441 [Abstract]  
• Denis, G. V., Vaziri, C., Guo, N., Faller, D. V. (2000). RING3 Kinase Transactivates Promoters of Cell Cycle Regulatory Genes through E2F. Cell Growth Differ. 11: 417-424 [Abstract] [Full Text]  
• de la Serna, I. L., Carlson, K. A., Hill, D. A., Guidi, C. J., Stephenson, R. O., Sif, S., Kingston, R. E., Imbalzano, A. N. (2000). Mammalian SWI-SNF Complexes Contribute to Activation of the hsp70 Gene. Mol. Cell. Biol. 20: 2839-2851 [Abstract] [Full Text]  
• Vignali, M., Hassan, A. H., Neely, K. E., Workman, J. L. (2000). ATP-Dependent Chromatin-Remodeling Complexes. Mol. Cell. Biol. 20: 1899-1910 [Full Text]  
• Bochar, D. A., Savard, J., Wang, W., Lafleur, D. W., Moore, P., Cote, J., Shiekhattar, R. (2000). A family of chromatin remodeling factors related to Williams syndrome transcription factor. Proc. Natl. Acad. Sci. USA 97: 1038-1043 [Abstract] [Full Text]  
• Ito, T., Levenstein, M. E., Fyodorov, D. V., Kutach, A. K., Kobayashi, R., Kadonaga, J. T. (1999). ACF consists of two subunits, Acf1 and ISWI, that function cooperatively in the ATP-dependent catalysis of chromatin assembly. Genes Dev. 13: 1529-1539 [Abstract] [Full Text]  
• Murphy, D. J., Hardy, S., Engel, D. A. (1999). Human SWI-SNF Component BRG1 Represses Transcription of the c-fos Gene. Mol. Cell. Biol. 19: 2724-2733 [Abstract] [Full Text]  
• Tsukiyama, T., Palmer, J., Landel, C. C., Shiloach, J., Wu, C. (1999). Characterization of the Imitation Switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae. Genes Dev. 13: 686-697 [Abstract] [Full Text]  
• Crosby, M. A., Miller, C., Alon, T., Watson, K. L., Verrijzer, C. P., Goldman-Levi, R., Zak, N. B. (1999). The trithorax Group Gene moira Encodes a Brahma-Associated Putative Chromatin-Remodeling Factor in Drosophila melanogaster. Mol. Cell. Biol. 19: 1159-1170 [Abstract] [Full Text]  
• Daubresse, G, Deuring, R, Moore, L, Papoulas, O, Zakrajsek, I, Waldrip, W., Scott, M., Kennison, J., Tamkun, J. (1999). The Drosophila kismet gene is related to chromatin-remodeling factors and is required for both segmentation and segment identity. Development 126: 1175-1187 [Abstract]  
• Auble, D. T., Steggerda, S. M. (1999). Testing for DNA Tracking by MOT1, a SNF2/SWI2 Protein Family Member. Mol. Cell. Biol. 19: 412-423 [Abstract] [Full Text]  
• LeRoy, G., Orphanides, G., Lane, W. S., Reinberg, D. (1998). Requirement of RSF and FACT for Transcription of Chromatin Templates in Vitro. Science 282: 1900-1904 [Abstract] [Full Text]  
• Kobayashi, M., Aita, N., Hayashi, S., Okada, K., Ohta, T., Hirose, S. (1998). DNA Supercoiling Factor Localizes to Puffs on Polytene Chromosomes in Drosophila melanogaster. Mol. Cell. Biol. 18: 6737-6744 [Abstract] [Full Text]  
• Gdula, D. A., Sandaltzopoulos, R., Tsukiyama, T., Ossipow, V., Wu, C. (1998). Inorganic pyrophosphatase is a component of the Drosophila nucleosome remodeling factor complex. Genes Dev. 12: 3206-3216 [Abstract] [Full Text]  
• Okada, M., Hirose, S. (1998). Chromatin Remodeling Mediated by Drosophila GAGA Factor and ISWI Activates fushi tarazu Gene Transcription In Vitro. Mol. Cell. Biol. 18: 2455-2461 [Abstract] [Full Text]  
• Cote, J., Peterson, C. L., Workman, J. L. (1998). Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding. Proc. Natl. Acad. Sci. USA 95: 4947-4952 [Abstract] [Full Text]  
• KENNISON, J. A., VAZQUEZ, M., BRIZUELA, B. J. (1998). Regulation of the Sex Combs Reduced Gene in Drosophila. Ann. N. Y. Acad. Sci. 842: 28-35 [Abstract] [Full Text]  
• Sukhodolets, M. V., Jin, D. J. (1998). RapA, a Novel RNA Polymerase-Associated Protein, Is a Bacterial Homolog of SWI2/SNF2. J. Biol. Chem. 273: 7018-7023 [Abstract] [Full Text]  
• Papoulas, O, Beek, S., Moseley, S., McCallum, C., Sarte, M, Shearn, A, Tamkun, J. (1998). The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. Development 125: 3955-3966 [Abstract]  
• (1998). Genetic Analysis of brahma: The Drosophila Homolog of the Yeast Chromatin Remodeling Factor SWI2/SNF2. Genetics 148: 251-266  
• Wu, C. (1997). Chromatin Remodeling and the Control of Gene Expression. J. Biol. Chem. 272: 28171-28174 [Full Text]  
• Imbalzano, A. N., Schnitzler, G. R., Kingston, R. E. (1996). Nucleosome Disruption by Human SWI/SNF Is Maintained in the Absence of Continued ATP Hydrolysis. J. Biol. Chem. 271: 20726-20733 [Abstract] [Full Text]  
• Kingston, R E, Bunker, C A, Imbalzano, A N (1996). Repression and activation by multiprotein complexes that alter chromatin structure.. Genes Dev. 10: 905-920 [Abstract]  
• Kruger, W, Peterson, C L, Sil, A, Coburn, C, Arents, G, Moudrianakis, E N, Herskowitz, I (1995). Amino acid substitutions in the structured domains of histones H3 and H4 partially relieve the requirement of the yeast SWI/SNF complex for transcription.. Genes Dev. 9: 2770-2779 [Abstract]  
• Sheridan, P. L., Schorpp, M., Voz, M. L., Jones, K. A. (1995). Cloning of an SNF2/SWI2-related Protein That Binds Specifically to the SPH Motifs of the SV40 Enhancer and to the HIV-1 Promoter. J. Biol. Chem. 270: 4575-4587 [Abstract] [Full Text]  
• Auble, D T, Hansen, K E, Mueller, C G, Lane, W S, Thorner, J, Hahn, S (1994). Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.. Genes Dev. 8: 1920-1934 [Abstract]  
• Cote, J, Quinn, J, Workman, J., Peterson, C. (1994). Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex. Science 265: 53-60 [Abstract]  
• Boyer, L. A., Logie, C., Bonte, E., Becker, P. B., Wade, P. A., Wolffe, A. P., Wu, C., Imbalzano, A. N., Peterson, C. L. (2000). Functional Delineation of Three Groups of the ATP-dependent Family of Chromatin Remodeling Enzymes. J. Biol. Chem. 275: 18864-18870 [Abstract] [Full Text]  
• Aalfs, J. D., Narlikar, G. J., Kingston, R. E. (2001). Functional Differences between the Human ATP-dependent Nucleosome Remodeling Proteins BRG1 and SNF2H. J. Biol. Chem. 276: 34270-34278 [Abstract] [Full Text]