This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Muslin, A J
Right arrow Articles by Williams, L T
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muslin, A J
Right arrow Articles by Williams, L T

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 May; 14(5): 3006-3012

Direct activation of phospholipase C-gamma by fibroblast growth factor receptor is not required for mesoderm induction in Xenopus animal caps.

A J Muslin, K G Peters and L T Williams

Program of Excellence in Molecular Biology, University of California, San Francisco 94143-0130.

ABSTRACT

Members of the fibroblast growth factor (FGF) family induce mesoderm formation in explants of Xenopus embryonic ectoderm (animal caps). Recent studies have been directed at determining signaling pathways downstream of the FGF receptor that are important in mesoderm induction. We have recently shown that a point mutation in the FGF receptor changing tyrosine 766 to phenylalanine (Y/F mutation) abolishes phospholipase C-gamma (PLC-gamma) activation in mammalian cells. To explore the role of PLC-gamma activation in FGF-stimulated mesoderm induction, we constructed two chimeric receptors, each consisting of the extracellular portion of the platelet-derived growth factor beta receptor, with one having the transmembrane and intracellular portions of the wild-type FGF receptor 1 (PR-FR wt) and the other having the corresponding region of the Y/F766 mutant FGF receptor 1 (PR-FR Y/F766). When expressed in Xenopus oocytes, only PR-FR wt was able to mediate PLC gamma phosphorylation, inositol-1,4,5-trisphosphate accumulation, and calcium efflux in response to platelet-derived growth factor stimulation. However, both receptors mediated mesoderm induction in Xenopus animal caps as measured by cap elongation, muscle-specific actin mRNA induction, and skeletal muscle formation. These results demonstrate that PLC gamma activation by the FGF receptor is not required for FGF-stimulated mesoderm induction.

Mol Cell Biol. 1994 May; 14(5): 3006-3012




This article has been cited by other articles:

  • Lonic, A., Barry, E. F., Quach, C., Kobe, B., Saunders, N., Guthridge, M. A. (2008). Fibroblast Growth Factor Receptor 2 Phosphorylation on Serine 779 Couples to 14-3-3 and Regulates Cell Survival and Proliferation. Mol. Cell. Biol. 28: 3372-3385 [Abstract] [Full Text]  
  • Whitaker, M. (2006). Calcium at Fertilization and in Early Development. Physiol. Rev. 86: 25-88 [Abstract] [Full Text]  
  • Park, E. K., Warner, N., Mood, K., Pawson, T., Daar, I. O. (2002). Low-Molecular-Weight Protein Tyrosine Phosphatase Is a Positive Component of the Fibroblast Growth Factor Receptor Signaling Pathway. Mol. Cell. Biol. 22: 3404-3414 [Abstract] [Full Text]  
  • Wang, F., McKeehan, K., Yu, C., McKeehan, W. L. (2002). Fibroblast Growth Factor Receptor 1 Phosphotyrosine 766: Molecular Target for Prevention of Progression of Prostate Tumors to Malignancy. Cancer Res. 62: 1898-1903 [Abstract] [Full Text]  
  • Nutt, S. L., Dingwell, K. S., Holt, C. E., Amaya, E. (2001). Xenopus Sprouty2 inhibits FGF-mediated gastrulation movements but does not affect mesoderm induction and patterning. Genes Dev. 15: 1152-1166 [Abstract] [Full Text]  
  • Morrison, T., Waggoner, L., Whitworth-Langley, L., Stith, B. J. (2000). Nongenomic Action of Progesterone: Activation of XenopusOocyte Phospholipase C through a Plasma Membrane-Associated Tyrosine Kinase. Endocrinology 141: 2145-2152 [Abstract] [Full Text]  
  • Umbhauer, M, Penzo-Mendez, A, Clavilier, L, Boucaut, J, Riou, J (2000). Signaling specificities of fibroblast growth factor receptors in early Xenopus embryo. J. Cell Sci. 113: 2865-2875 [Abstract]  
  • Wu, C, Zeng, Q, Blumer, K., Muslin, A. (2000). RGS proteins inhibit Xwnt-8 signaling in Xenopus embryonic development. Development 127: 2773-2784 [Abstract]  
  • Dvorak, P, Hampl, A, Jirmanova, L, Pacholikova, J, Kusakabe, M (1998). Embryoglycan ectodomains regulate biological activity of FGF-2 to embryonic stem cells. J. Cell Sci. 111: 2945-2952 [Abstract]  
  • Bikfalvi, A., Klein, S., Pintucci, G., Rifkin, D. B. (1997). Biological Roles of Fibroblast Growth Factor-2. Endocr. Rev. 18: 26-45 [Abstract] [Full Text]  
  • Ault, K., Durmowicz, G, Galione, A, Harger, P., Busa, W. (1996). Modulation of Xenopus embryo mesoderm-specific gene expression and dorsoanterior patterning by receptors that activate the phosphatidylinositol cycle signal transduction pathway. Development 122: 2033-2041 [Abstract]  
  • Kim, S., Lakhani, V., Costa, D. J., Sharara, A. I., Fitz, J. G., Huang, L.-W., Peters, K. G., Kindman, L. A. (1995). Sphingolipid-gated Ca[IMAGE] Release from Intracellular Stores of Endothelial Cells Is Mediated by a Novel Ca[IMAGE]-permeable Channel. J. Biol. Chem. 270: 5266-5269 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»