This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hisamoto, N
Right arrow Articles by Matsumoto, K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hisamoto, N
Right arrow Articles by Matsumoto, K

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 May; 14(5): 3158-3165

The Glc7 type 1 protein phosphatase of Saccharomyces cerevisiae is required for cell cycle progression in G2/M.

N Hisamoto, K Sugimoto and K Matsumoto

Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.

ABSTRACT

We isolated a mutant carrying a conditional mutation in the GLC7 gene, encoding the catalytic subunit of a type 1 protein phosphatase, by selection of suppressors that restored the growth defect of cdc24 mutants at high temperature and simultaneously conferred cold-sensitive growth. This cold sensitivity for growth is caused by a single mutation (glc7Y-170) at position 170 of the Glc7 protein, resulting in replacement of cysteine with tyrosine. Genetic analysis suggested that the glc7Y-170 allele is associated with a recessive negative phenotype, reducing the activity of Glc7 in the cell. The glc7Y-170 mutant missegregated chromosome III at the permissive temperature, arrested growth as large-budded cells at the restrictive temperature, exhibited a significant increase in the number of nuclei at or in the neck, and had a short spindle. Furthermore, the glc7Y-170 mutant exhibited a high level of CDC28-dependent protein kinase activity when incubated at the restrictive temperature. These findings suggest that the glc7Y-170 mutation is defective in the G2/M phase of the cell cycle. Thus, type 1 protein phosphatase in Saccharomyces cerevisiae is essential for the G2/M transition.

Mol Cell Biol. 1994 May; 14(5): 3158-3165




This article has been cited by other articles:

  • Wang, W., Cronmiller, C., Brautigan, D. L. (2008). Maternal Phosphatase Inhibitor-2 Is Required for Proper Chromosome Segregation and Mitotic Synchrony During Drosophila Embryogenesis. Genetics 179: 1823-1833 [Abstract] [Full Text]  
  • Bharucha, J. P., Larson, J. R., Gao, L., Daves, L. K., Tatchell, K. (2008). Ypi1, a Positive Regulator of Nuclear Protein Phosphatase Type 1 Activity in Saccharomyces cerevisiae. Mol. Biol. Cell 19: 1032-1045 [Abstract] [Full Text]  
  • Zeng, G., Huang, B., Neo, S. P., Wang, J., Cai, M. (2007). Scd5p Mediates Phosphoregulation of Actin and Endocytosis by the Type 1 Phosphatase Glc7p in Yeast. Mol. Biol. Cell 18: 4885-4898 [Abstract] [Full Text]  
  • Pedelini, L., Marquina, M., Arino, J., Casamayor, A., Sanz, L., Bollen, M., Sanz, P., Garcia-Gimeno, M. A. (2007). YPI1 and SDS22 Proteins Regulate the Nuclear Localization and Function of Yeast Type 1 Phosphatase Glc7. J. Biol. Chem. 282: 3282-3292 [Abstract] [Full Text]  
  • Dessauge, F., Cayla, X., Albar, J. P., Fleischer, A., Ghadiri, A., Duhamel, M., Rebollo, A. (2006). Identification of PP1{alpha} as a Caspase-9 Regulator in IL-2 Deprivation-Induced Apoptosis. J. Immunol. 177: 2441-2451 [Abstract] [Full Text]  
  • Pinsky, B. A., Kotwaliwale, C. V., Tatsutani, S. Y., Breed, C. A., Biggins, S. (2006). Glc7/Protein phosphatase 1 regulatory subunits can oppose the ipl1/aurora protein kinase by redistributing glc7.. Mol. Cell. Biol. 26: 2648-2660 [Abstract] [Full Text]  
  • Santangelo, G. M. (2006). Glucose Signaling in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 70: 253-282 [Abstract] [Full Text]  
  • Chang, J. S., Henry, K., Geli, M. I., Lemmon, S. K. (2006). Cortical Recruitment and Nuclear-Cytoplasmic Shuttling of Scd5p, a Protein Phosphatase-1-targeting Protein Involved in Actin Organization and Endocytosis. Mol. Biol. Cell 17: 251-262 [Abstract] [Full Text]  
  • Cui, D.-Y., Brown, C. R., Chiang, H.-L. (2004). The Type 1 Phosphatase Reg1p-Glc7p Is Required for the Glucose-induced Degradation of Fructose-1,6-bisphosphatase in the Vacuole. J. Biol. Chem. 279: 9713-9724 [Abstract] [Full Text]  
  • CEULEMANS, H., BOLLEN, M. (2004). Functional Diversity of Protein Phosphatase-1, a Cellular Economizer and Reset Button. Physiol. Rev. 84: 1-39 [Abstract] [Full Text]  
  • Garcia-Gimeno, M. A., Munoz, I., Arino, J., Sanz, P. (2003). Molecular Characterization of Ypi1, a Novel Saccharomyces cerevisiae Type 1 Protein Phosphatase Inhibitor. J. Biol. Chem. 278: 47744-47752 [Abstract] [Full Text]  
  • Leach, C., Shenolikar, S., Brautigan, D. L. (2003). Phosphorylation of Phosphatase Inhibitor-2 at Centrosomes during Mitosis. J. Biol. Chem. 278: 26015-26020 [Abstract] [Full Text]  
  • Kim, Y.-M., Watanabe, T., Allen, P. B., Kim, Y.-M., Lee, S.-J., Greengard, P., Nairn, A. C., Kwon, Y.-G. (2003). PNUTS, a Protein Phosphatase 1 (PP1) Nuclear Targeting Subunit. CHARACTERIZATION OF ITS PP1- AND RNA-BINDING DOMAINS AND REGULATION BY PHOSPHORYLATION. J. Biol. Chem. 278: 13819-13828 [Abstract] [Full Text]  
  • Tan, Y. S. H., Morcos, P. A., Cannon, J. F. (2003). Pho85 Phosphorylates the Glc7 Protein Phosphatase Regulator Glc8 in Vivo. J. Biol. Chem. 278: 147-153 [Abstract] [Full Text]  
  • Wang, H., Brautigan, D. L. (2002). A Novel Transmembrane Ser/Thr Kinase Complexes with Protein Phosphatase-1 and Inhibitor-2. J. Biol. Chem. 277: 49605-49612 [Abstract] [Full Text]  
  • Mayordomo, I., Estruch, F., Sanz, P. (2002). Convergence of the Target of Rapamycin and the Snf1 Protein Kinase Pathways in the Regulation of the Subcellular Localization of Msn2, a Transcriptional Activator of STRE (Stress Response Element)-regulated Genes. J. Biol. Chem. 277: 35650-35656 [Abstract] [Full Text]  
  • Glickman, M. H., Ciechanover, A. (2002). The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction. Physiol. Rev. 82: 373-428 [Abstract] [Full Text]  
  • Venturi, G. M., Bloecher, A., Williams-Hart, T., Tatchell, K. (2000). Genetic Interactions Between GLC7, PPZ1 and PPZ2 in Saccharomyces cerevisiae. Genetics 155: 69-83 [Abstract] [Full Text]  
  • Bloecher, A., Tatchell, K. (2000). Dynamic Localization of Protein Phosphatase Type 1 in the Mitotic Cell Cycle of Saccharomyces cerevisiae. JCB 149: 125-140 [Abstract] [Full Text]  
  • Sanz, P., Alms, G. R., Haystead, T. A. J., Carlson, M. (2000). Regulatory Interactions between the Reg1-Glc7 Protein Phosphatase and the Snf1 Protein Kinase. Mol. Cell. Biol. 20: 1321-1328 [Abstract] [Full Text]  
  • Cheng, A., Dean, N. M., Honkanen, R. E. (2000). Serine/Threonine Protein Phosphatase Type 1gamma 1 Is Required for the Completion of Cytokinesis in Human A549 Lung Carcinoma Cells. J. Biol. Chem. 275: 1846-1854 [Abstract] [Full Text]  
  • Andrews, P., Stark, M. (2000). Type 1 protein phosphatase is required for maintenance of cell wall integrity, morphogenesis and cell cycle progression in Saccharomyces cerevisiae. J. Cell Sci. 113: 507-520 [Abstract]  
  • Burns, C. G., Ohi, R., Krainer, A. R., Gould, K. L. (1999). Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Proc. Natl. Acad. Sci. USA 96: 13789-13794 [Abstract] [Full Text]  
  • Bloecher, A., Tatchell, K. (1999). Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint. Genes Dev. 13: 517-522 [Abstract] [Full Text]  
  • Biggins, S., Severin, F. F., Bhalla, N., Sassoon, I., Hyman, A. A., Murray, A. W. (1999). The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast. Genes Dev. 13: 532-544 [Abstract] [Full Text]  
  • Sassoon, I., Severin, F. F., Andrews, P. D., Taba, M.-R., Kaplan, K. B., Ashford, A. J., Stark, M. J.R., Sorger, P. K., Hyman, A. A. (1999). Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p. Genes Dev. 13: 545-555 [Abstract] [Full Text]  
  • Vorlaufer, E., Peters, J.-M. (1998). Regulation of the Cyclin B Degradation System by an Inhibitor of Mitotic Proteolysis. Mol. Biol. Cell 9: 1817-1831 [Abstract] [Full Text]  
  • Ramaswamy, N. T., Li, L., Khalil, M., Cannon, J. F. (1998). Regulation of Yeast Glycogen Metabolism and Sporulation by Glc7p Protein Phosphatase. Genetics 149: 57-72 [Abstract] [Full Text]  
  • Allen, P. B., Kwon, Y.-G., Nairn, A. C., Greengard, P. (1998). Isolation and Characterization of PNUTS, a Putative Protein Phosphatase 1 Nuclear Targeting Subunit. J. Biol. Chem. 273: 4089-4095 [Abstract] [Full Text]  
  • Kwon, Y.-G., Lee, S. Y., Choi, Y., Greengard, P., Nairn, A. C. (1997). Cell cycle-dependent phosphorylation of mammalian protein phosphatase 1 by cdc2 kinase. Proc. Natl. Acad. Sci. USA 94: 2168-2173 [Abstract] [Full Text]  
  • King, R. W., Deshaies, R. J., Peters, J.-M., Kirschner, M. W. (1996). How Proteolysis Drives the Cell Cycle. Science 274: 1652-1659 [Abstract] [Full Text]  
  • Bastians, H, Ponstingl, H (1996). The novel human protein serine/threonine phosphatase 6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, which are involved in cell cycle regulation. J. Cell Sci. 109: 2865-2874 [Abstract]  
  • Zheng, J., Khalil, M., Cannon, J. F. (2000). Glc7p Protein Phosphatase Inhibits Expression of Glutamine-Fructose-6-phosphate Transaminase from GFA1. J. Biol. Chem. 275: 18070-18078 [Abstract] [Full Text]  
  • Friedman, D. B., Kern, J. W., Huneycutt, B. J., Vinh, D. B. N., Crawford, D. K., Steiner, E., Scheiltz, D., Yates, J. III, Resing, K. A., Ahn, N. G., Winey, M., Davis, T. N. (2001). Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain. J. Biol. Chem. 276: 17958-17967 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»