This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schneider, C
Right arrow Articles by Fanning, E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schneider, C
Right arrow Articles by Fanning, E

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 May; 14(5): 3176-3185

Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA.

C Schneider, K Weisshart, L A Guarino, I Dornreiter and E Fanning

Institute for Biochemistry, Munich, Germany.

ABSTRACT

Physical and functional interactions of simian virus 40 (SV40) and polyomavirus large-T antigens with DNA polymerase alpha-primase were analyzed to elucidate the molecular basis for the species specificity of polymerase alpha-primase in viral DNA replication. SV40 T antigen associated more efficiently with polymerase alpha-primase in crude human extracts than in mouse extracts, while polyomavirus T antigen interacted preferentially with polymerase alpha-primase in mouse extracts. The apparent species specificity of complex formation was not observed when purified polymerase alpha-primases were substituted for the crude extracts. Several functional interactions between T antigen and purified polymerase alpha-primase, including stimulation of primer synthesis and primer elongation on M13 DNA in the presence or absence of the single-stranded DNA binding protein RP-A, also proved to be independent of the species from which polymerase alpha-primase had been purified. However, the human DNA polymerase alpha-primase was specifically required for primosome assembly and primer synthesis on SV40 origin DNA in the presence of T antigen and RP-A.

Mol Cell Biol. 1994 May; 14(5): 3176-3185




This article has been cited by other articles:

  • Mahon, C., Liang, B., Tikhanovich, I., Abend, J. R., Imperiale, M. J., Nasheuer, H. P., Folk, W. R. (2009). Restriction of Human Polyomavirus BK Virus DNA Replication in Murine Cells and Extracts. J. Virol. 83: 5708-5717 [Abstract] [Full Text]  
  • Weisshart, K., Pestryakov, P., Smith, R. W. P., Hartmann, H., Kremmer, E., Lavrik, O., Nasheuer, H.-P. (2004). Coordinated Regulation of Replication Protein A Activities by Its Subunits p14 and p32. J. Biol. Chem. 279: 35368-35376 [Abstract] [Full Text]  
  • Fien, K., Cho, Y.-S., Lee, J.-K., Raychaudhuri, S., Tappin, I., Hurwitz, J. (2004). Primer Utilization by DNA Polymerase {alpha}-Primase Is Influenced by Its Interaction with Mcm10p. J. Biol. Chem. 279: 16144-16153 [Abstract] [Full Text]  
  • Ott, R. D., Rehfuess, C., Podust, V. N., Clark, J. E., Fanning, E. (2002). Role of the p68 Subunit of Human DNA Polymerase {alpha}-Primase in Simian Virus 40 DNA Replication. Mol. Cell. Biol. 22: 5669-5678 [Abstract] [Full Text]  
  • Smith, R. W. P., Steffen, C., Grosse, F., Nasheuer, H.-P. (2002). Species Specificity of Simian Virus 40 DNA Replication in Vitro Requires Multiple Functions of Human DNA Polymerase alpha. J. Biol. Chem. 277: 20541-20548 [Abstract] [Full Text]  
  • Ott, R. D., Wang, Y., Fanning, E. (2002). Mutational Analysis of Simian Virus 40 T-Antigen Primosome Activities in Viral DNA Replication. J. Virol. 76: 5121-5130 [Abstract] [Full Text]  
  • Eom, C.-Y., Lehman, I. R. (2002). The human DnaJ protein, hTid-1, enhances binding of a multimer of the herpes simplex virus type 1 UL9 protein to oris, an origin of viral DNA replication. Proc. Natl. Acad. Sci. USA 99: 1894-1898 [Abstract] [Full Text]  
  • Kautz, A. R., Weisshart, K., Schneider, A., Grosse, F., Nasheuer, H.-P. (2001). Amino Acids 257 to 288 of Mouse p48 Control the Cooperation of Polyomavirus Large T Antigen, Replication Protein A, and DNA Polymerase {alpha}-Primase To Synthesize DNA In Vitro. J. Virol. 75: 8569-8578 [Abstract] [Full Text]  
  • Kautz, A. r., Schneider, A., Weisshart, K., Geiger, C., Nasheuer, H.-P. (2001). Different Regions of Primase Subunit p48 Control Mouse Polyomavirus and Simian Virus 40 DNA Replication In Vitro. J. Virol. 75: 1751-1760 [Abstract] [Full Text]  
  • Han, Y., Loo, Y.-M., Militello, K. T., Melendy, T. (1999). Interactions of the Papovavirus DNA Replication Initiator Proteins, Bovine Papillomavirus Type 1 E1 and Simian Virus 40 Large T Antigen, with Human Replication Protein A. J. Virol. 73: 4899-4907 [Abstract] [Full Text]  
  • Reynisdóttir, I., Bhattacharyya, S., Zhang, D., Prives, C. (1999). The Retinoblastoma Protein Alters the Phosphorylation State of Polyomavirus Large T Antigen in Murine Cell Extracts and Inhibits Polyomavirus Origin DNA Replication. J. Virol. 73: 3004-3013 [Abstract] [Full Text]  
  • Voitenleitner, C., Rehfuess, C., Hilmes, M., O'Rear, L., Liao, P.-C., Gage, D. A., Ott, R., Nasheuer, H.-P., Fanning, E. (1999). Cell Cycle-Dependent Regulation of Human DNA Polymerase alpha -Primase Activity by Phosphorylation. Mol. Cell. Biol. 19: 646-656 [Abstract] [Full Text]  
  • Weisshart, K., Taneja, P., Fanning, E. (1998). The Replication Protein A Binding Site in Simian Virus 40 (SV40) T Antigen and Its Role in the Initial Steps of SV40 DNA Replication. J. Virol. 72: 9771-9781 [Abstract] [Full Text]  
  • Boche, I., Fanning, E. (1997). Nucleocytoplasmic Recycling of the Nuclear Localization Signal Receptor {alpha} Subunit In Vivo Is Dependent on a Nuclear Export Signal, Energy, and RCC1. JCB 139: 313-325 [Abstract] [Full Text]  
  • Dickmanns, A, Bischoff, F., Marshallsay, C, Luhrmann, R, Ponstingl, H, Fanning, E (1996). The thermolability of nuclear protein import in tsBN2 cells is suppressed by microinjected Ran-GTP or Ran-GDP, but not by RanQ69L or RanT24N. J. Cell Sci. 109: 1449-1457 [Abstract]  
  • D'Urso, G, Grallert, B, Nurse, P (1995). DNA polymerase alpha, a component of the replication initiation complex, is essential for the checkpoint coupling S phase to mitosis in fission yeast. J. Cell Sci. 108: 3109-3118 [Abstract]  
  • Weisshart, K., Forster, H., Kremmer, E., Schlott, B., Grosse, F., Nasheuer, H.-P. (2000). Protein-Protein Interactions of the Primase Subunits p58 and p48 with Simian Virus 40 T Antigen Are Required for Efficient Primer Synthesis in a Cell-free System. J. Biol. Chem. 275: 17328-17337 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»