This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bardwell, A J Articles by Friedberg, E C Search for Related Content PubMed PubMed Citation Articles by Bardwell, A J Articles by Friedberg, E C

Yeast nucleotide excision repair proteins Rad2 and Rad4 interact with RNA polymerase II basal transcription factor b (TFIIH).

Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235.

ABSTRACT

The Rad2, Rad3, Rad4, and Ss12 proteins are required for nucleotide excision repair in yeast cells and are homologs of four human proteins which are involved in a group of hereditary repair-defective diseases. We have previously shown that Rad3 protein is one of the five subunits of purified RNA polymerase II basal transcription initiation factor b (TFIIH) and that Ss12 protein physically associates with factor b (W.J. Feaver, J.Q. Svejstrup, L. Bardwell, A.J. Bardwell, S. Buratowski, K.D. Gulyas, T.F. Donahue, E.C. Friedberg, and R.D. Kornberg, Cell 75:1379-1387, 1993). Here we show that the Rad2 and Rad4 proteins interact with purified factor b in vitro. Rad2 (a single-stranded DNA endonuclease) specifically interacts with the Tfb1 subunit of factor b, and we have mapped a limited region of the Rad2 polypeptide which is sufficient for this interaction. Rad2 also interacts directly with Ss12 protein (a putative DNA helicase). The binding of Rad2 and Rad4 proteins to factor b may define intermediates in the assembly of the nucleotide excision repair repairosome. Furthermore, the loading of factor b (or such intermediates) onto promoters during transcription initiation provides a mechanism for the preferential targeting of repair proteins to actively transcribing genes.

This article has been cited by other articles:

• Zhou, Y., Kou, H., Wang, Z. (2007). Tfb5 interacts with Tfb2 and facilitates nucleotide excision repair in yeast. Nucleic Acids Res 35: 861-871 [Abstract] [Full Text]  
• Araújo, S. J., Nigg, E. A., Wood, R. D. (2001). Strong Functional Interactions of TFIIH with XPC and XPG in Human DNA Nucleotide Excision Repair, without a Preassembled Repairosome. Mol. Cell. Biol. 21: 2281-2291 [Abstract] [Full Text]  
• Yokoi, M., Masutani, C., Maekawa, T., Sugasawa, K., Ohkuma, Y., Hanaoka, F. (2000). The Xeroderma Pigmentosum Group C Protein Complex XPC-HR23B Plays an Important Role in the Recruitment of Transcription Factor IIH to Damaged DNA. J. Biol. Chem. 275: 9870-9875 [Abstract] [Full Text]  
• Feaver, W. J., Huang, W., Gileadi, O., Myers, L., Gustafsson, C. M., Kornberg, R. D., Friedberg, E. C. (2000). Subunit Interactions in Yeast Transcription/Repair Factor TFIIH. REQUIREMENT FOR Tfb3 SUBUNIT IN NUCLEOTIDE EXCISION REPAIR. J. Biol. Chem. 275: 5941-5946 [Abstract] [Full Text]  
• Miyamoto, T., Kakizawa, T., Hashizume, K. (1999). Inhibition of Nuclear Receptor Signalling by Poly(ADP-Ribose) Polymerase. Mol. Cell. Biol. 19: 2644-2649 [Abstract] [Full Text]  
• de Laat, W. L., Jaspers, N. G.J., Hoeijmakers, J. H.J. (1999). Molecular mechanism of nucleotide excision repair. Genes Dev. 13: 768-785 [Full Text]  
• Jansen, L. E.T., Verhage, R. A., Brouwer, J. (1998). Preferential Binding of Yeast Rad4·Rad23 Complex to Damaged DNA. J. Biol. Chem. 273: 33111-33114 [Abstract] [Full Text]  
• Tantin, D. (1998). RNA Polymerase II Elongation Complexes Containing the Cockayne Syndrome Group B Protein Interact with a Molecular Complex Containing the Transcription Factor IIH Components Xeroderma Pigmentosum B and p62. J. Biol. Chem. 273: 27794-27799 [Abstract] [Full Text]  
• Bardwell, L., Cook, J. G., Voora, D., Baggott, D. M., Martinez, A. R., Thorner, J. (1998). Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK. Genes Dev. 12: 2887-2898 [Abstract] [Full Text]  
• Hampsey, M. (1998). Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery. Microbiol. Mol. Biol. Rev. 62: 465-503 [Abstract] [Full Text]  
• Ahn, B., Grossman, L. (1996). RNA Polymerase Signals UvrAB Landing Sites. J. Biol. Chem. 271: 21453-21461 [Abstract] [Full Text]  
• Guzder, S. N., Sung, P., Prakash, L., Prakash, S. (1996). Nucleotide Excision Repair in Yeast Is Mediated by Sequential Assembly of Repair Factors and Not by a Pre-assembled Repairosome. J. Biol. Chem. 271: 8903-8910 [Abstract] [Full Text]  
• Mu, D., Park, C.-H., Matsunaga, T., Hsu, D. S., Reardon, J. T., Sancar, A. (1995). Reconstitution of Human DNA Repair Excision Nuclease in a Highly Defined System. J. Biol. Chem. 270: 2415-2418 [Abstract] [Full Text]  
• Bardwell, A., Bardwell, L, Tomkinson, A., Friedberg, E. (1994). Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease. Science 265: 2082-2085 [Abstract]