This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dufort, D
Right arrow Articles by Nepveu, A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dufort, D
Right arrow Articles by Nepveu, A

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 June; 14(6): 4251-4257

The human cut homeodomain protein represses transcription from the c-myc promoter.

D Dufort and A Nepveu

Department of Medicine, McGill University, Royal Victoria Hospital, Montreal, Quebec, Canada.

ABSTRACT

Studies of the c-myc promoter have shown that efficient transcription initiation at the P2 start site as well as the block to elongation of transcription require the presence of the ME1a1 protein binding site upstream of the P2 TATA box. Following fractionation by size exclusion chromatography, three protein-ME1a1 DNA complexes, a, b, and c, were detected by electrophoretic mobility shift assay. A cDNA encoding a protein present in complex c was isolated by screening of an expression library with an ME1a1 DNA probe. This cDNA was found to encode the human homolog of the Drosophila Cut homeodomain protein. The bacterially expressed human Cut (hu-Cut) protein bound to the ME1a1 site, and antibodies against hu-Cut inhibited the ME1a1 binding activity c in nuclear extracts. In cotransfection experiments, the hu-Cut protein repressed transcription from the c-myc promoter, and this repression was shown to be dependent on the presence of the ME1a1 site. Using a reporter construct with a heterologous promoter, we found that c-myc exon 1 sequences were also necessary, in addition to the ME1a1 site, for repression by Cut. Taken together, these results suggest that the human homolog of the Drosophila Cut homeodomain protein is involved in regulation of the c-myc gene.

Mol Cell Biol. 1994 June; 14(6): 4251-4257




This article has been cited by other articles:

  • Cadieux, C., Harada, R., Paquet, M., Cote, O., Trudel, M., Nepveu, A., Bouchard, M. (2008). Polycystic Kidneys Caused by Sustained Expression of Cux1 Isoform p75. J. Biol. Chem. 283: 13817-13824 [Abstract] [Full Text]  
  • Maitra, U., Seo, J., Lozano, M. M., Dudley, J. P. (2006). Differentiation-Induced Cleavage of Cutl1/CDP Generates a Novel Dominant-Negative Isoform That Regulates Mammary Gene Expression. Mol. Cell. Biol. 26: 7466-7478 [Abstract] [Full Text]  
  • Cadieux, C., Fournier, S., Peterson, A. C., Bedard, C., Bedell, B. J., Nepveu, A. (2006). Transgenic Mice Expressing the p75 CCAAT-Displacement Protein/Cut Homeobox Isoform Develop a Myeloproliferative Disease-Like Myeloid Leukemia. Cancer Res. 66: 9492-9501 [Abstract] [Full Text]  
  • Belanger, H., Beaulieu, P., Moreau, C., Labuda, D., Hudson, T. J., Sinnett, D. (2005). Functional promoter SNPs in cell cycle checkpoint genes. Hum Mol Genet 14: 2641-2648 [Abstract] [Full Text]  
  • Perez-Romero, P., Tyler, R. E., Abend, J. R., Dus, M., Imperiale, M. J. (2005). Analysis of the Interaction of the Adenovirus L1 52/55-Kilodalton and IVa2 Proteins with the Packaging Sequence In Vivo and In Vitro. J. Virol. 79: 2366-2374 [Abstract] [Full Text]  
  • Ferrante, R. J., Ryu, H., Kubilus, J. K., D'Mello, S., Sugars, K. L., Lee, J., Lu, P., Smith, K., Browne, S., Beal, M. F., Kristal, B. S., Stavrovskaya, I. G., Hewett, S., Rubinsztein, D. C., Langley, B., Ratan, R. R. (2004). Chemotherapy for the Brain: The Antitumor Antibiotic Mithramycin Prolongs Survival in a Mouse Model of Huntington's Disease. J. Neurosci. 24: 10335-10342 [Abstract] [Full Text]  
  • Nishio, H., Walsh, M. J. (2004). CCAAT displacement protein/cut homolog recruits G9a histone lysine methyltransferase to repress transcription. Proc. Natl. Acad. Sci. USA 101: 11257-11262 [Abstract] [Full Text]  
  • Zhu, Q., Maitra, U., Johnston, D., Lozano, M., Dudley, J. P. (2004). The Homeodomain Protein CDP Regulates Mammary-Specific Gene Transcription and Tumorigenesis. Mol. Cell. Biol. 24: 4810-4823 [Abstract] [Full Text]  
  • Truscott, M., Raynal, L., Premdas, P., Goulet, B., Leduy, L., Berube, G., Nepveu, A. (2003). CDP/Cux Stimulates Transcription from the DNA Polymerase {alpha} Gene Promoter. Mol. Cell. Biol. 23: 3013-3028 [Abstract] [Full Text]  
  • Boudreau, F., Rings, E. H. H. M., Swain, G. P., Sinclair, A. M., Suh, E. R., Silberg, D. G., Scheuermann, R. H., Traber, P. G. (2002). A Novel Colonic Repressor Element Regulates Intestinal Gene Expression by Interacting with Cux/CDP. Mol. Cell. Biol. 22: 5467-5478 [Abstract] [Full Text]  
  • Zhu, Q., Dudley, J. P. (2002). CDP Binding to Multiple Sites in the Mouse Mammary Tumor Virus Long Terminal Repeat Suppresses Basal and Glucocorticoid-Induced Transcription. J. Virol. 76: 2168-2179 [Abstract] [Full Text]  
  • Luong, M. X., van der Meijden, C. M., Xing, D., Hesselton, R., Monuki, E. S., Jones, S. N., Lian, J. B., Stein, J. L., Stein, G. S., Neufeld, E. J., van Wijnen, A. J. (2002). Genetic Ablation of the CDP/Cux Protein C Terminus Results in Hair Cycle Defects and Reduced Male Fertility. Mol. Cell. Biol. 22: 1424-1437 [Abstract] [Full Text]  
  • Santaguida, M., Ding, Q., Berube, G., Truscott, M., Whyte, P., Nepveu, A. (2001). Phosphorylation of the CCAAT Displacement Protein (CDP)/Cux Transcription Factor by Cyclin A-Cdk1 Modulates Its DNA Binding Activity in G2. J. Biol. Chem. 276: 45780-45790 [Abstract] [Full Text]  
  • Moon, N. S., Premdas, P., Truscott, M., Leduy, L., Berube, G., Nepveu, A. (2001). S Phase-Specific Proteolytic Cleavage Is Required To Activate Stable DNA Binding by the CDP/Cut Homeodomain Protein. Mol. Cell. Biol. 21: 6332-6345 [Abstract] [Full Text]  
  • Khanna-Gupta, A., Zibello, T., Sun, H., Lekstrom-Himes, J., Berliner, N. (2001). C/EBPvarepsilon mediates myeloid differentiation and is regulated by the CCAAT displacement protein (CDP/cut). Proc. Natl. Acad. Sci. USA 98: 8000-8005 [Abstract] [Full Text]  
  • Zhu, Q., Gregg, K., Lozano, M., Liu, J., Dudley, J. P. (2000). CDP Is a Repressor of Mouse Mammary Tumor Virus Expression in the Mammary Gland. J. Virol. 74: 6348-6357 [Abstract] [Full Text]  
  • Li, S., Aufiero, B., Schiltz, R. L., Walsh, M. J. (2000). Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein (CBP)-associated factor and CBP. Proc. Natl. Acad. Sci. USA 10.1073/pnas.130028697v1 [Abstract] [Full Text]  
  • van Gurp, M. F., Pratap, J., Luong, M., Javed, A., Hoffmann, H., Giordano, A., Stein, J. L., Neufeld, E. J., Lian, J. B., Stein, G. S., van Wijnen, A. J. (1999). The CCAAT Displacement Protein/cut Homeodomain Protein Represses Osteocalcin Gene Transcription and Forms Complexes with the Retinoblastoma Protein-related Protein p107 and Cyclin A. Cancer Res. 59: 5980-5988 [Abstract] [Full Text]  
  • Catt, D., Hawkins, S., Roman, A., Luo, W., Skalnik, D. G. (1999). Overexpression of CCAAT Displacement Protein Represses the Promiscuously Active Proximal gp91phox Promoter. Blood 94: 3151-3160 [Abstract] [Full Text]  
  • Jacobsen, B. M., Skalnik, D. G. (1999). YY1 Binds Five cis-Elements and Trans-activates the Myeloid Cell-restricted gp91phox Promoter. J. Biol. Chem. 274: 29984-29993 [Abstract] [Full Text]  
  • Izzo, M. W., Strachan, G. D., Stubbs, M. C., Hall, D. J. (1999). Transcriptional Repression from the c-myc P2 Promoter by the Zinc Finger Protein ZF87/MAZ. J. Biol. Chem. 274: 19498-19506 [Abstract] [Full Text]  
  • Liu, J., Barnett, A., Neufeld, E. J., Dudley, J. P. (1999). Homeoproteins CDP and SATB1 Interact: Potential for Tissue-Specific Regulation. Mol. Cell. Biol. 19: 4918-4926 [Abstract] [Full Text]  
  • Ai, W., Toussaint, E., Roman, A. (1999). CCAAT Displacement Protein Binds to and Negatively Regulates Human Papillomavirus Type 6 E6, E7, and E1 Promoters. J. Virol. 73: 4220-4229 [Abstract] [Full Text]  
  • Li, S., Moy, L., Pittman, N., Shue, G., Aufiero, B., Neufeld, E. J., LeLeiko, N. S., Walsh, M. J. (1999). Transcriptional Repression of the Cystic Fibrosis Transmembrane Conductance Regulator Gene, Mediated by CCAAT Displacement Protein/cut Homolog, Is Associated with Histone Deacetylation. J. Biol. Chem. 274: 7803-7815 [Abstract] [Full Text]  
  • Wang, Z., Goldstein, A., Zong, R.-T., Lin, D., Neufeld, E. J., Scheuermann, R. H., Tucker, P. W. (1999). Cux/CDP Homeoprotein Is a Component of NF-µNR and Represses the Immunoglobulin Heavy Chain Intronic Enhancer by Antagonizing the Bright Transcription Activator. Mol. Cell. Biol. 19: 284-295 [Abstract] [Full Text]  
  • Valentine, S. A., Chen, G., Shandala, T., Fernandez, J., Mische, S., Saint, R., Courey, A. J. (1998). Dorsal-Mediated Repression Requires the Formation of a Multiprotein Repression Complex at the Ventral Silencer. Mol. Cell. Biol. 18: 6584-6594 [Abstract] [Full Text]  
  • Glöckner, G., Scherer, S., Schattevoy, R., Boright, A., Weber, J., Tsui, L.-C., Rosenthal, A. (1998). Large-Scale Sequencing of Two Regions in Human Chromosome 7q22: Analysis of 650 kb of Genomic Sequence around the EPO and CUTL1 Loci Reveals 17 Genes. Genome Res 8: 1060-1073 [Abstract] [Full Text]  
  • Coqueret, O., Martin, N., Berube, G., Rabbat, M., Litchfield, D. W., Nepveu, A. (1998). DNA Binding by Cut Homeodomain Proteins Is Down-modulated by Casein Kinase II. J. Biol. Chem. 273: 2561-2566 [Abstract] [Full Text]  
  • Pullner, A., Mautner, J., Albert, T., Eick, D. (1996). Nucleosomal Structure of Active and Inactive c-myc Genes. J. Biol. Chem. 271: 31452-31457 [Abstract] [Full Text]  
  • Coqueret, O., Berube, G., Nepveu, A. (1996). DNA Binding by Cut Homeodomain Proteins Is Down-modulated by Protein Kinase C. J. Biol. Chem. 271: 24862-24868 [Abstract] [Full Text]  
  • Lievens, P. M. J., Donady, J. J., Tufarelli, C., Neufeld, E. J. (1995). Repressor Activity of CCAAT Displacement Protein in HL-60 Myeloid Leukemia Cells. J. Biol. Chem. 270: 12745-12750 [Abstract] [Full Text]  
  • Brewster, R, Bodmer, R (1995). Origin and specification of type II sensory neurons in Drosophila. Development 121: 2923-2936 [Abstract]  
  • Snyder, S. R., Wang, J., Waring, J. F., Ginder, G. D. (2001). Identification of CCAAT Displacement Protein (CDP/cut) as a Locus-specific Repressor of Major Histocompatibility Complex Gene Expression in Human Tumor Cells. J. Biol. Chem. 276: 5323-5330 [Abstract] [Full Text]  
  • Albert, T., Wells, J., Funk, J.-O., Pullner, A., Raschke, E.-E., Stelzer, G., Meisterernst, M., Farnham, P. J., Eick, D. (2001). The Chromatin Structure of the Dual c-myc Promoter P1/P2 Is Regulated by Separate Elements. J. Biol. Chem. 276: 20482-20490 [Abstract] [Full Text]  
  • Li, S., Aufiero, B., Schiltz, R. L., Walsh, M. J. (2000). Regulation of the homeodomain CCAAT displacement/cut protein function by histone acetyltransferases p300/CREB-binding protein (CBP)-associated factor and CBP. Proc. Natl. Acad. Sci. USA 97: 7166-7171 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»