This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Francisco, L
Right arrow Articles by Chan, C S
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Francisco, L
Right arrow Articles by Chan, C S

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 July; 14(7): 4731-4740

Type 1 protein phosphatase acts in opposition to IpL1 protein kinase in regulating yeast chromosome segregation.

L Francisco, W Wang and C S Chan

Department of Microbiology, University of Texas, Austin 78712.

ABSTRACT

The IPL1 gene is required for high-fidelity chromosome segregation in the budding yeast Saccharomyces cerevisiae. Conditional ipl1ts mutants missegregate chromosomes severely at 37 degrees C. Here, we report that IPL1 encodes an essential putative protein kinase whose function is required during the later part of each cell cycle. At 26 degrees C, the permissive growth temperature, ipl1 mutant cells are defective in the recovery from a transient G2/M-phase arrest caused by the antimicrotubule drug nocodazole. In an effort to identify additional gene products that participate with the Ipl1 protein kinase in regulating chromosome segregation in yeast, a truncated version of the previously identified DIS2S1/GLC7 gene was isolated as a dosage-dependent suppressor of ipl1ts mutations. DIS2S1/GLC7 is predicted to encode a catalytic subunit (PP1C) of type 1 protein phosphatase. Overexpression of the full-length DIS2S1/GLC7 gene results in chromosome missegregation in wild-type cells and exacerbates the mutant phenotype in ipl1 cells. In addition, the glc7-1 mutation can partially suppress the ipl1-1 mutation. These results suggest that type 1 protein phosphatase acts in opposition to the Ipl1 protein kinase in vivo to ensure the high fidelity of chromosome segregation.

Mol Cell Biol. 1994 July; 14(7): 4731-4740




This article has been cited by other articles:

  • Ishihara, M., Suda, Y., Inoue, I., Tanaka, T., Takahashi, T., Gao, X.-D., Fukui, Y., Ihara, S., Neiman, A. M., Tachikawa, H. (2009). Protein Phosphatase Type 1-Interacting Protein Ysw1 Is Involved in Proper Septin Organization and Prospore Membrane Formation during Sporulation. Eukaryot Cell 8: 1027-1037 [Abstract] [Full Text]  
  • Wang, W., Stukenberg, P. T., Brautigan, D. L. (2008). Phosphatase Inhibitor-2 Balances Protein Phosphatase 1 and Aurora B Kinase for Chromosome Segregation and Cytokinesis in Human Retinal Epithelial Cells. Mol. Biol. Cell 19: 4852-4862 [Abstract] [Full Text]  
  • de Carvalho, C. E., Zaaijer, S., Smolikov, S., Gu, Y., Schumacher, J. M., Colaiacovo, M. P. (2008). LAB-1 antagonizes the Aurora B kinase in C. elegans. Genes Dev. 22: 2869-2885 [Abstract] [Full Text]  
  • Jwa, M., Kim, J.-h., Chan, C. S.M. (2008). Regulation of Sli15/INCENP, kinetochore, and Cdc14 phosphatase functions by the ribosome biogenesis protein Utp7. JCB 182: 1099-1111 [Abstract] [Full Text]  
  • Wang, W., Cronmiller, C., Brautigan, D. L. (2008). Maternal Phosphatase Inhibitor-2 Is Required for Proper Chromosome Segregation and Mitotic Synchrony During Drosophila Embryogenesis. Genetics 179: 1823-1833 [Abstract] [Full Text]  
  • Emanuele, M. J., Lan, W., Jwa, M., Miller, S. A., Chan, C. S.M., Stukenberg, P. T. (2008). Aurora B kinase and protein phosphatase 1 have opposing roles in modulating kinetochore assembly. JCB 181: 241-254 [Abstract] [Full Text]  
  • Bharucha, J. P., Larson, J. R., Gao, L., Daves, L. K., Tatchell, K. (2008). Ypi1, a Positive Regulator of Nuclear Protein Phosphatase Type 1 Activity in Saccharomyces cerevisiae. Mol. Biol. Cell 19: 1032-1045 [Abstract] [Full Text]  
  • Zeng, G., Huang, B., Neo, S. P., Wang, J., Cai, M. (2007). Scd5p Mediates Phosphoregulation of Actin and Endocytosis by the Type 1 Phosphatase Glc7p in Yeast. Mol. Biol. Cell 18: 4885-4898 [Abstract] [Full Text]  
  • Alvarez-Tabares, I., Grallert, A., Ortiz, J.-M., Hagan, I. M. (2007). Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth. J. Cell Sci. 120: 3589-3601 [Abstract] [Full Text]  
  • Briassouli, P., Chan, F., Savage, K., Reis-Filho, J. S., Linardopoulos, S. (2007). Aurora-A Regulation of Nuclear Factor-{kappa}B Signaling by Phosphorylation of I{kappa}B{alpha}. Cancer Res. 67: 1689-1695 [Abstract] [Full Text]  
  • Pedelini, L., Marquina, M., Arino, J., Casamayor, A., Sanz, L., Bollen, M., Sanz, P., Garcia-Gimeno, M. A. (2007). YPI1 and SDS22 Proteins Regulate the Nuclear Localization and Function of Yeast Type 1 Phosphatase Glc7. J. Biol. Chem. 282: 3282-3292 [Abstract] [Full Text]  
  • Briassouli, P., Chan, F., Linardopoulos, S. (2006). The N-terminal domain of the Aurora-A Phe-31 variant encodes an E3 ubiquitin ligase and mediates ubiquitination of I{kappa}B{alpha}. Hum Mol Genet 15: 3343-3350 [Abstract] [Full Text]  
  • Pinsky, B. A., Kotwaliwale, C. V., Tatsutani, S. Y., Breed, C. A., Biggins, S. (2006). Glc7/Protein phosphatase 1 regulatory subunits can oppose the ipl1/aurora protein kinase by redistributing glc7.. Mol. Cell. Biol. 26: 2648-2660 [Abstract] [Full Text]  
  • Vazquez-Novelle, M. D., Esteban, V., Bueno, A., Sacristan, M. P. (2005). Functional Homology among Human and Fission Yeast Cdc14 Phosphatases. J. Biol. Chem. 280: 29144-29150 [Abstract] [Full Text]  
  • Fukuda, T., Mishina, Y., Walker, M. P., DiAugustine, R. P. (2005). Conditional Transgenic System for Mouse Aurora A Kinase: Degradation by the Ubiquitin Proteasome Pathway Controls the Level of the Transgenic Protein. Mol. Cell. Biol. 25: 5270-5281 [Abstract] [Full Text]  
  • Liu, Q., Kaneko, S., Yang, L., Feldman, R. I., Nicosia, S. V., Chen, J., Cheng, J. Q. (2004). Aurora-A Abrogation of p53 DNA Binding and Transactivation Activity by Phosphorylation of Serine 215. J. Biol. Chem. 279: 52175-52182 [Abstract] [Full Text]  
  • Ruiz, A., Munoz, I., Serrano, R., Gonzalez, A., Simon, E., Arino, J. (2004). Functional Characterization of the Saccharomyces cerevisiae VHS3 Gene: A REGULATORY SUBUNIT OF THE Ppz1 PROTEIN PHOSPHATASE WITH NOVEL, PHOSPHATASE-UNRELATED FUNCTIONS. J. Biol. Chem. 279: 34421-34430 [Abstract] [Full Text]  
  • Sun, C., Newbatt, Y., Douglas, L., Workman, P., Aherne, W., Linardopoulos, S. (2004). High-Throughput Screening Assay for Identification of Small Molecule Inhibitors of Aurora2/STK15 Kinase. J Biomol Screen 9: 391-397 [Abstract]  
  • Yao, L.-J., Zhong, Z.-S., Zhang, L.-S., Chen, D.-Y., Schatten, H., Sun, Q.-Y. (2004). Aurora-A Is a Critical Regulator of Microtubule Assembly and Nuclear Activity in Mouse Oocytes, Fertilized Eggs, and Early Embryos. Biol. Reprod. 70: 1392-1399 [Abstract] [Full Text]  
  • Yasui, Y., Urano, T., Kawajiri, A., Nagata, K.-i., Tatsuka, M., Saya, H., Furukawa, K., Takahashi, T., Izawa, I., Inagaki, M. (2004). Autophosphorylation of a Newly Identified Site of Aurora-B Is Indispensable for Cytokinesis. J. Biol. Chem. 279: 12997-13003 [Abstract] [Full Text]  
  • Jeng, Y.-M., Peng, S.-Y., Lin, C.-Y., Hsu, H.-C. (2004). Overexpression and Amplification of Aurora-A in Hepatocellular Carcinoma. Clin. Cancer Res. 10: 2065-2071 [Abstract] [Full Text]  
  • Cui, D.-Y., Brown, C. R., Chiang, H.-L. (2004). The Type 1 Phosphatase Reg1p-Glc7p Is Required for the Glucose-induced Degradation of Fructose-1,6-bisphosphatase in the Vacuole. J. Biol. Chem. 279: 9713-9724 [Abstract] [Full Text]  
  • CEULEMANS, H., BOLLEN, M. (2004). Functional Diversity of Protein Phosphatase-1, a Cellular Economizer and Reset Button. Physiol. Rev. 84: 1-39 [Abstract] [Full Text]  
  • Marumoto, T., Honda, S., Hara, T., Nitta, M., Hirota, T., Kohmura, E., Saya, H. (2003). Aurora-A Kinase Maintains the Fidelity of Early and Late Mitotic Events in HeLa Cells. J. Biol. Chem. 278: 51786-51795 [Abstract] [Full Text]  
  • Garcia-Gimeno, M. A., Munoz, I., Arino, J., Sanz, P. (2003). Molecular Characterization of Ypi1, a Novel Saccharomyces cerevisiae Type 1 Protein Phosphatase Inhibitor. J. Biol. Chem. 278: 47744-47752 [Abstract] [Full Text]  
  • Prigent, C., Dimitrov, S. (2003). Phosphorylation of serine 10 in histone H3, what for?. J. Cell Sci. 116: 3677-3685 [Abstract] [Full Text]  
  • Honda, R., Korner, R., Nigg, E. A. (2003). Exploring the Functional Interactions between Aurora B, INCENP, and Survivin in Mitosis. Mol. Biol. Cell 14: 3325-3341 [Abstract] [Full Text]  
  • Maton, G., Thibier, C., Castro, A., Lorca, T., Prigent, C., Jessus, C. (2003). Cdc2-Cyclin B Triggers H3 Kinase Activation of Aurora-A in Xenopus Oocytes. J. Biol. Chem. 278: 21439-21449 [Abstract] [Full Text]  
  • Ma, C., Cummings, C., Liu, X. J. (2003). Biphasic Activation of Aurora-A Kinase during the Meiosis I- Meiosis II Transition in Xenopus Oocytes. Mol. Cell. Biol. 23: 1703-1716 [Abstract] [Full Text]  
  • Li, D., Zhu, J., Firozi, P. F., Abbruzzese, J. L., Evans, D. B., Cleary, K., Friess, H., Sen, S. (2003). Overexpression of Oncogenic STK15/BTAK/Aurora A Kinase in Human Pancreatic Cancer. Clin. Cancer Res. 9: 991-997 [Abstract] [Full Text]  
  • Buvelot, S., Tatsutani, S. Y., Vermaak, D., Biggins, S. (2003). The budding yeast Ipl1/Aurora protein kinase regulates mitotic spindle disassembly. JCB 160: 329-339 [Abstract] [Full Text]  
  • Tan, Y. S. H., Morcos, P. A., Cannon, J. F. (2003). Pho85 Phosphorylates the Glc7 Protein Phosphatase Regulator Glc8 in Vivo. J. Biol. Chem. 278: 147-153 [Abstract] [Full Text]  
  • Trinkle-Mulcahy, L., Andrews, P. D., Wickramasinghe, S., Sleeman, J., Prescott, A., Lam, Y. W., Lyon, C., Swedlow, J. R., Lamond, A. I. (2003). Time-lapse Imaging Reveals Dynamic Relocalization of PP1gamma throughout the Mammalian Cell Cycle. Mol. Biol. Cell 14: 107-117 [Abstract] [Full Text]  
  • Sakai, H., Urano, T., Ookata, K., Kim, M.-H., Hirai, Y., Saito, M., Nojima, Y., Ishikawa, F. (2002). MBD3 and HDAC1, Two Components of the NuRD Complex, Are Localized at Aurora-A-positive Centrosomes in M Phase. J. Biol. Chem. 277: 48714-48723 [Abstract] [Full Text]  
  • Ceulemans, H., Vulsteke, V., De Maeyer, M., Tatchell, K., Stalmans, W., Bollen, M. (2002). Binding of the Concave Surface of the Sds22 Superhelix to the alpha 4/alpha 5/alpha 6-Triangle of Protein Phosphatase-1. J. Biol. Chem. 277: 47331-47337 [Abstract] [Full Text]  
  • Kiat, L. S., Hui, K. M., Gopalan, G. (2002). Aurora-A Kinase Interacting Protein (AIP), a Novel Negative Regulator of Human Aurora-A Kinase. J. Biol. Chem. 277: 45558-45565 [Abstract] [Full Text]  
  • Mayordomo, I., Estruch, F., Sanz, P. (2002). Convergence of the Target of Rapamycin and the Snf1 Protein Kinase Pathways in the Regulation of the Subcellular Localization of Msn2, a Transcriptional Activator of STRE (Stress Response Element)-regulated Genes. J. Biol. Chem. 277: 35650-35656 [Abstract] [Full Text]  
  • Sen, S., Zhou, H., Zhang, R.-D., Yoon, D. S., Vakar-Lopez, F., Ito, S., Jiang, F., Johnston, D., Grossman, H. B., Ruifrok, A. C., Katz, R. L., Brinkley, W., Czerniak, B. (2002). Amplification/Overexpression of a Mitotic Kinase Gene in Human Bladder Cancer. JNCI J Natl Cancer Inst 94: 1320-1329 [Abstract] [Full Text]  
  • Rogers, E., Bishop, J. D., Waddle, J. A., Schumacher, J. M., Lin, R. (2002). The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis. JCB 157: 219-229 [Abstract] [Full Text]  
  • Petersen, J., Paris, J., Willer, M., Philippe, M., Hagan, I. M. (2002). The S. pombe aurora-related kinase Ark1 associates with mitotic structures in a stage dependent manner and is required for chromosome segregation. J. Cell Sci. 114: 4371-4384 [Abstract] [Full Text]  
  • Peggie, M. W., MacKelvie, S. H., Bloecher, A., Knatko, E. V., Tatchell, K., Stark, M. J. R. (2002). Essential functions of Sds22p in chromosome stability and nuclear localization of PP1. J. Cell Sci. 115: 195-206 [Abstract] [Full Text]  
  • Biggins, S., Bhalla, N., Chang, A., Smith, D. L., Murray, A. W. (2001). Genes Involved in Sister Chromatid Separation and Segregation in the Budding Yeast Saccharomyces cerevisiae. Genetics 159: 453-470 [Abstract] [Full Text]  
  • Giet, R., Prigent, C. (2001). The non-catalytic domain of the Xenopus laevis auroraA kinase localises the protein to the centrosome. J. Cell Sci. 114: 2095-2104 [Abstract] [Full Text]  
  • Velmurugan, S., Yang, X.-M., Chan, C. S.-M., Dobson, M., Jayaram, M. (2000). Partitioning of the 2-{micro}m Circle Plasmid of Saccharomyces cerevisiae: Functional Coordination with Chromosome Segregation and Plasmid-Encoded Rep Protein Distribution. JCB 149: 553-566 [Abstract] [Full Text]  
  • Venturi, G. M., Bloecher, A., Williams-Hart, T., Tatchell, K. (2000). Genetic Interactions Between GLC7, PPZ1 and PPZ2 in Saccharomyces cerevisiae. Genetics 155: 69-83 [Abstract] [Full Text]  
  • Sanz, P., Alms, G. R., Haystead, T. A. J., Carlson, M. (2000). Regulatory Interactions between the Reg1-Glc7 Protein Phosphatase and the Snf1 Protein Kinase. Mol. Cell. Biol. 20: 1321-1328 [Abstract] [Full Text]  
  • Kim, J.-h., Kang, J.-s., Chan, C. S.M. (1999). Sli15 Associates with the Ipl1 Protein Kinase to Promote Proper Chromosome Segregation in Saccharomyces cerevisiae. JCB 145: 1381-1394 [Abstract] [Full Text]  
  • Farruggio, D. C., Townsley, F. M., Ruderman, J. V. (1999). Cdc20 associates with the kinase aurora2/Aik. Proc. Natl. Acad. Sci. USA 96: 7306-7311 [Abstract] [Full Text]  
  • Giet, R., Uzbekov, R., Cubizolles, F., Le Guellec, K., Prigent, C. (1999). The Xenopus laevis Aurora-related Protein Kinase pEg2 Associates with and Phosphorylates the Kinesin-related Protein XlEg5. J. Biol. Chem. 274: 15005-15013 [Abstract] [Full Text]  
  • Tanaka, T., Kimura, M., Matsunaga, K., Fukada, D., Mori, H., Okano, Y. (1999). Centrosomal Kinase AIK1 Is Overexpressed in Invasive Ductal Carcinoma of the Breast. Cancer Res. 59: 2041-2044 [Abstract] [Full Text]  
  • Sandrock, T. M., Brower, S. M., Toenjes, K. A., Adams, A. E. M. (1999). Suppressor Analysis of Fimbrin (Sac6p) Overexpression in Yeast. Genetics 151: 1287-1297 [Abstract] [Full Text]  
  • Kimura, M., Matsuda, Y., Yoshioka, T., Okano, Y. (1999). Cell Cycle-dependent Expression and Centrosome Localization of a Third Human Aurora/Ipl1-related Protein Kinase, AIK3. J. Biol. Chem. 274: 7334-7340 [Abstract] [Full Text]  
  • Biggins, S., Severin, F. F., Bhalla, N., Sassoon, I., Hyman, A. A., Murray, A. W. (1999). The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast. Genes Dev. 13: 532-544 [Abstract] [Full Text]  
  • Sassoon, I., Severin, F. F., Andrews, P. D., Taba, M.-R., Kaplan, K. B., Ashford, A. J., Stark, M. J.R., Sorger, P. K., Hyman, A. A. (1999). Regulation of Saccharomyces cerevisiae kinetochores by the type 1 phosphatase Glc7p. Genes Dev. 13: 545-555 [Abstract] [Full Text]  
  • Giet, R, Prigent, C (1999). Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases. J. Cell Sci. 112: 3591-3601 [Abstract]  
  • Schumacher, J. M., Golden, A., Donovan, P. J. (1998). AIR-2: An Aurora/Ipl1-related Protein Kinase Associated with Chromosomes and Midbody Microtubules Is Required for Polar Body Extrusion and Cytokinesis in Caenorhabditis elegans Embryos. JCB 143: 1635-1646 [Abstract] [Full Text]  
  • Schumacher, J., Ashcroft, N, Donovan, P., Golden, A (1998). A highly conserved centrosomal kinase, AIR-1, is required for accurate cell cycle progression and segregation of developmental factors in Caenorhabditis elegans embryos. Development 125: 4391-4402 [Abstract]  
  • Roghi, C, Giet, R, Uzbekov, R, Morin, N, Chartrain, I, Le Guellec, R, Couturier, A, Doree, M, Philippe, M, Prigent, C (1998). The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly. J. Cell Sci. 111: 557-572 [Abstract]  
  • Luca, F. C., Winey, M. (1998). MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy. Mol. Biol. Cell 9: 29-46 [Abstract] [Full Text]  
  • Gopalan, G., Chan, C. S.M., Donovan, P. J. (1997). A Novel Mammalian, Mitotic Spindle-associated Kinase Is Related to Yeast and Fly Chromosome Segregation Regulators. JCB 138: 643-656 [Abstract] [Full Text]  
  • Kimura, M., Kotani, S., Hattori, T., Sumi, N., Yoshioka, T., Todokoro, K., Okano, Y. (1997). Cell Cycle-dependent Expression and Spindle Pole Localization of a Novel Human Protein Kinase, Aik, Related to Aurora of Drosophila and Yeast Ipl1. J. Biol. Chem. 272: 13766-13771 [Abstract] [Full Text]  
  • Zheng, J., Khalil, M., Cannon, J. F. (2000). Glc7p Protein Phosphatase Inhibits Expression of Glutamine-Fructose-6-phosphate Transaminase from GFA1. J. Biol. Chem. 275: 18070-18078 [Abstract] [Full Text]  
  • Pan, J., Snell, W. J. (2000). Regulated Targeting of a Protein Kinase into an Intact Flagellum. AN AURORA/Ipl1p-LIKE PROTEIN KINASE TRANSLOCATES FROM THE CELL BODY INTO THE FLAGELLA DURING GAMETE ACTIVATION IN CHLAMYDOMONAS. J. Biol. Chem. 275: 24106-24114 [Abstract] [Full Text]  
  • Tang, C.-J. C., Chuang, C.-K., Hu, H.-M., Tang, T. K. (2001). The Zinc Finger Domain of Tzfp Binds to the tbs Motif Located at the Upstream Flanking Region of the Aie1 (aurora-C) Kinase Gene. J. Biol. Chem. 276: 19631-19639 [Abstract] [Full Text]  
  • Murnion, M. E., Adams, R. R., Callister, D. M., Allis, C. D., Earnshaw, W. C., Swedlow, J. R. (2001). Chromatin-associated Protein Phosphatase 1 Regulates Aurora-B and Histone H3 Phosphorylation. J. Biol. Chem. 276: 26656-26665 [Abstract] [Full Text]  
  • Kang, J.-s., Cheeseman, I. M., Kallstrom, G., Velmurugan, S., Barnes, G., Chan, C. S.M. (2001). Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation. JCB 155: 763-774 [Abstract] [Full Text]  
  • Zeitlin, S. G., Shelby, R. D., Sullivan, K. F. (2001). CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis. JCB 155: 1147-1158 [Abstract] [Full Text]  
  • Rogers, E., Bishop, J. D., Waddle, J. A., Schumacher, J. M., Lin, R. (2002). The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis. JCB 157: 219-229 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»