This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Engelberg, D
Right arrow Articles by Karin, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Engelberg, D
Right arrow Articles by Karin, M

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 July; 14(7): 4929-4937

The yeast and mammalian Ras pathways control transcription of heat shock genes independently of heat shock transcription factor.

D Engelberg, E Zandi, C S Parker and M Karin

Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093-0636.

ABSTRACT

Yeast strains in which the Ras-cyclic AMP (cAMP) pathway is constitutively active are sensitive to heat shock, whereas mutants in which the activity of this pathway is low are hyperresistant to heat shock. To determine the molecular basis for these differences, we examined the transcriptional induction of heat shock genes in various yeast strains. Activation of heat shock genes was attenuated in the strains in which the Ras-cAMP pathway is constitutively active. In contrast, in a strain deficient in cAMP production, several heat shock genes were induced by removal of cAMP from the medium. These results indicate that the Ras-cAMP pathway affects the induction of heat shock genes. In all of the mutants, heat shock transcription factor expression and activity were identical to those in wild-type cells. The response to heat shock in Ha-ras-transformed rat fibroblasts was also studied. While no induction of Hsp68 was observed in Ha-ras-transformed cells, proper regulation of heat shock transcription factor was found. Therefore, in mammals, as in Saccharomyces cerevisiae, the Ras pathway controls the transcription of heat shock genes via a mechanism not involving the heat shock transcription factor.

Mol Cell Biol. 1994 July; 14(7): 4929-4937




This article has been cited by other articles:

  • Belden, W. J., Larrondo, L. F., Froehlich, A. C., Shi, M., Chen, C.-H., Loros, J. J., Dunlap, J. C. (2007). The band mutation in Neurospora crassa is a dominant allele of ras-1 implicating RAS signaling in circadian output. Genes Dev. 21: 1494-1505 [Abstract] [Full Text]  
  • Ferguson, S. B., Anderson, E. S., Harshaw, R. B., Thate, T., Craig, N. L., Nelson, H. C. M. (2005). Protein Kinase A Regulates Constitutive Expression of Small Heat-Shock Genes in an Msn2/4p-Independent and Hsf1p-Dependent Manner in Saccharomyces cerevisiae. Genetics 169: 1203-1214 [Abstract] [Full Text]  
  • Ivey, F. D., Kays, A. M., Borkovich, K. A. (2002). Shared and Independent Roles for a G{alpha}i Protein and Adenylyl Cyclase in Regulating Development and Stress Responses in Neurospora crassa. Eukaryot Cell 1: 634-642 [Abstract] [Full Text]  
  • Scherz, R., Shinder, V., Engelberg, D. (2001). Anatomical Analysis of Saccharomyces cerevisiae Stalk-Like Structures Reveals Spatial Organization and Cell Specialization. J. Bacteriol. 183: 5402-5413 [Abstract] [Full Text]  
  • Piu, F., Aronheim, A., Katz, S., Karin, M. (2001). AP-1 Repressor Protein JDP-2: Inhibition of UV-Mediated Apoptosis through p53 Down-Regulation. Mol. Cell. Biol. 21: 3012-3024 [Abstract] [Full Text]  
  • Vertrees, R. A., Zwischenberger, J. B., Boor, P. J., Pencil, S. D. (2000). Oncogenic ras results in increased cell kill due to defective thermoprotection in lung cancer cells. Ann. Thorac. Surg. 69: 1675-1680 [Abstract] [Full Text]  
  • Stanhill, A., Schick, N., Engelberg, D. (1999). The Yeast Ras/Cyclic AMP Pathway Induces Invasive Growth by Suppressing the Cellular Stress Response. Mol. Cell. Biol. 19: 7529-7538 [Abstract] [Full Text]  
  • Chu, B., Zhong, R., Soncin, F., Stevenson, M. A., Calderwood, S. K. (1998). Transcriptional Activity of Heat Shock Factor 1 at 37 oC Is Repressed through Phosphorylation on Two Distinct Serine Residues by Glycogen Synthase Kinase 3alpha and Protein Kinases Calpha and Czeta. J. Biol. Chem. 273: 18640-18646 [Abstract] [Full Text]  
  • Boy-Marcotte, E., Perrot, M., Bussereau, F., Boucherie, H., Jacquet, M. (1998). Msn2p and Msn4p Control a Large Number of Genes Induced at the Diauxic Transition Which Are Repressed by Cyclic AMP in Saccharomyces cerevisiae. J. Bacteriol. 180: 1044-1052 [Abstract] [Full Text]  
  • Holmberg, C., Roos, P., Lord, J., Eriksson, J., Sistonen, L (1998). Conventional and novel PKC isoenzymes modify the heat-induced stress response but are not activated by heat shock. J. Cell Sci. 111: 3357-3365 [Abstract]  
  • Huang, J., Nueda, A., Yoo, S., Dynan, W. S. (1997). Heat Shock Transcription Factor 1 Binds Selectively in Vitro to Ku Protein and the Catalytic Subunit of the DNA-dependent Protein Kinase. J. Biol. Chem. 272: 26009-26016 [Abstract] [Full Text]  
  • Chu, B., Soncin, F., Price, B. D., Stevenson, M. A., Calderwood, S. K. (1996). Sequential Phosphorylation by Mitogen-activated Protein Kinase and Glycogen Synthase Kinase 3Represses Transcriptional Activation by Heat Shock Factor-1. J. Biol. Chem. 271: 30847-30857 [Abstract] [Full Text]  
  • Knauf, U, Newton, E M, Kyriakis, J, Kingston, R E (1996). Repression of human heat shock factor 1 activity at control temperature by phosphorylation.. Genes Dev. 10: 2782-2793 [Abstract]  
  • Head, M., Hurwitz, L, Goldman, J. (1996). Transcription regulation of alpha B-crystallin in astrocytes: analysis of HSF and AP1 activation by different types of physiological stress. J. Cell Sci. 109: 1029-1039 [Abstract]  
  • Qian, Y.-W., Lee, E. Y.-H. P. (1995). Dual Retinoblastoma-binding Proteins with Properties Related to a Negative Regulator of Ras in Yeast. J. Biol. Chem. 270: 25507-25513 [Abstract] [Full Text]  
  • Marbach, I., Licht, R., Frohnmeyer, H., Engelberg, D. (2001). Gcn2 Mediates Gcn4 Activation in Response to Glucose Stimulation or UV Radiation Not via GCN4 Translation. J. Biol. Chem. 276: 16944-16951 [Abstract] [Full Text]  
  • Bell, M., Capone, R., Pashtan, I., Levitzki, A., Engelberg, D. (2001). Isolation of Hyperactive Mutants of the MAPK p38/Hog1 That Are Independent of MAPK Kinase Activation. J. Biol. Chem. 276: 25351-25358 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»