This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Genetta, T
Right arrow Articles by Kadesch, T
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Genetta, T
Right arrow Articles by Kadesch, T

 Previous Article  |  Next Article 

Mol Cell Biol. 1994 September; 14(9): 6153-6163

Displacement of an E-box-binding repressor by basic helix-loop-helix proteins: implications for B-cell specificity of the immunoglobulin heavy-chain enhancer.

T Genetta, D Ruezinsky and T Kadesch

Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19704.

ABSTRACT

The activity of the immunoglobulin heavy-chain (IgH) enhancer is restricted to B cells, although it binds both B-cell-restricted and ubiquitous transcription factors. Activation of the enhancer in non-B cells upon overexpression of the basic helix-loop-helix (bHLH) protein E2A appears to be mediated not only by the binding of E2A to its cognate E box but also by the resulting displacement of a repressor from that same site. We have identified a "two-handed" zinc finger protein, denoted ZEB, the DNA-binding specificity of which mimics that of the cellular repressor. By employing a derivative E box that binds ZEB but not E2A, we have shown that the repressor is active in B cells and the IgH enhancer is silenced in the absence of binding competition by bHLH proteins. Hence, we propose that a necessary prerequisite of enhancer activity is the B-cell-specific displacement of a ZEB-like repressor by bHLH proteins.

Mol Cell Biol. 1994 September; 14(9): 6153-6163




This article has been cited by other articles:

  • Bellon, E., Luyten, F. P., Tylzanowski, P. (2009). {delta}-EF1 is a negative regulator of Ihh in the developing growth plate. JCB 187: 685-699 [Abstract] [Full Text]  
  • Desai, S., Bolick, S. C. E., Maurin, M., Wright, K. L. (2009). PU.1 Regulates Positive Regulatory Domain I-Binding Factor 1/Blimp-1 Transcription in Lymphoma Cells. J. Immunol. 183: 5778-5787 [Abstract] [Full Text]  
  • Liu, Y., El-Naggar, S., Darling, D. S., Higashi, Y., Dean, D. C. (2008). Zeb1 links epithelial-mesenchymal transition and cellular senescence. Development 135: 579-588 [Abstract] [Full Text]  
  • Kowase, T., Walsh, H. E., Darling, D. S., Shupnik, M. A. (2007). Estrogen Enhances Gonadotropin-Releasing Hormone-Stimulated Transcription of the Luteinizing Hormone Subunit Promoters via Altered Expression of Stimulatory and Suppressive Transcription Factors. Endocrinology 148: 6083-6091 [Abstract] [Full Text]  
  • Feng, W.-h., Kraus, R. J., Dickerson, S. J., Lim, H. J., Jones, R. J., Yu, X., Mertz, J. E., Kenney, S. C. (2007). ZEB1 and c-Jun Levels Contribute to the Establishment of Highly Lytic Epstein-Barr Virus Infection in Gastric AGS Cells. J. Virol. 81: 10113-10122 [Abstract] [Full Text]  
  • Kiefer, H., Chatail-Hermitte, F., Ravassard, P., Bayard, E., Brunet, I., Mallet, J. (2005). ZENON, a Novel POZ Kruppel-Like DNA Binding Protein Associated with Differentiation and/or Survival of Late Postmitotic Neurons. Mol. Cell. Biol. 25: 1713-1729 [Abstract] [Full Text]  
  • Kaul-Ghanekar, R., Jalota, A., Pavithra, L., Tucker, P., Chattopadhyay, S. (2004). SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCR{beta} enhancer (E{beta}). Nucleic Acids Res 32: 4862-4875 [Abstract] [Full Text]  
  • Xu, P., LaVallee, P. A., Lin, J. J., Hoidal, J. R. (2004). Characterization of Proteins Binding to E-box/Ku86 Sites and Function of Ku86 in Transcriptional Regulation of the Human Xanthine Oxidoreductase Gene. J. Biol. Chem. 279: 16057-16063 [Abstract] [Full Text]  
  • Wacker, I., Schwarz, V., Hedgecock, E. M., Hutter, H. (2003). zag-1, a Zn-finger homeodomain transcription factor controlling neuronal differentiation and axon outgrowth in C. elegans. Development 130: 3795-3805 [Abstract] [Full Text]  
  • van Grunsven, L. A., Michiels, C., Van de Putte, T., Nelles, L., Wuytens, G., Verschueren, K., Huylebroeck, D. (2003). Interaction between Smad-interacting Protein-1 and the Corepressor C-terminal Binding Protein Is Dispensable for Transcriptional Repression of E-cadherin. J. Biol. Chem. 278: 26135-26145 [Abstract] [Full Text]  
  • Kraus, R. J., Perrigoue, J. G., Mertz, J. E. (2002). ZEB Negatively Regulates the Lytic-Switch BZLF1 Gene Promoter of Epstein-Barr Virus. J. Virol. 77: 199-207 [Abstract] [Full Text]  
  • Takanaka, Y., Okano, T., Yamamoto, K., Fukada, Y. (2002). A Negative Regulatory Element Required for Light-Dependent pinopsin Gene Expression. J. Neurosci. 22: 4357-4363 [Abstract] [Full Text]  
  • Fontemaggi, G., Gurtner, A., Strano, S., Higashi, Y., Sacchi, A., Piaggio, G., Blandino, G. (2001). The Transcriptional Repressor ZEB Regulates p73 Expression at the Crossroad between Proliferation and Differentiation. Mol. Cell. Biol. 21: 8461-8470 [Abstract] [Full Text]  
  • Lazarova, D. L., Bordonaro, M., Sartorelli, A. C. (2001). Transcriptional Regulation of the Vitamin D3 Receptor Gene by ZEB. Cell Growth Differ. 12: 319-326 [Abstract] [Full Text]  
  • van Grunsven, L. A., Schellens, A., Huylebroeck, D., Verschueren, K. (2001). SIP1 (Smad Interacting Protein 1) and {delta}EF1 ({delta}-Crystallin Enhancer Binding Factor) are Structurally Similar Transcriptional Repressors : A Current Survey of Their Functions and Mechanisms of Action in Transforming Growth Factor-{beta} Signalling. JBJS 83: S40-47 [Abstract] [Full Text]  
  • van Grunsven, L. A., Schellens, A., Huylebroeck, D., Verschueren, K. (2001). SIP1 (Smad Interacting Protein 1) and {delta}EF1 ({delta}-Crystallin Enhancer Binding Factor) are Structurally Similar Transcriptional Repressors : A Current Survey of Their Functions and Mechanisms of Action in Transforming Growth Factor-{beta} Signalling. JBJS 83: S40-S47 [Abstract] [Full Text]  
  • Rushlow, C., Colosimo, P. F., Lin, M.-c., Xu, M., Kirov, N. (2001). Transcriptional regulation of the Drosophila gene zen by competing Smad and Brinker inputs. Genes Dev. 15: 340-351 [Abstract] [Full Text]  
  • Kraus, R. J., Mirocha, S. J., Stephany, H. M., Puchalski, J. R., Mertz, J. E. (2001). Identification of a Novel Element Involved in Regulation of the Lytic Switch BZLF1 Gene Promoter of Epstein-Barr Virus. J. Virol. 75: 867-877 [Abstract] [Full Text]  
  • Tanaka, K., Matsumoto, Y., Nakatani, F., Iwamoto, Y., Yamada, Y. (2000). A Zinc Finger Transcription Factor, alpha A-Crystallin Binding Protein 1, Is a Negative Regulator of the Chondrocyte-Specific Enhancer of the alpha 1(II) Collagen Gene. Mol. Cell. Biol. 20: 4428-4435 [Abstract] [Full Text]  
  • Postigo, A. A., Dean, D. C. (2000). Differential expression and function of members of the zfh-1 family of zinc finger/homeodomain repressors. Proc. Natl. Acad. Sci. USA 97: 6391-6396 [Abstract] [Full Text]  
  • Murray, D., Precht, P., Balakir, R., Horton, W. E. Jr. (2000). The Transcription Factor delta EF1 Is Inversely Expressed with Type II Collagen mRNA and Can Repress Col2a1 Promoter Activity in Transfected Chondrocytes. J. Biol. Chem. 275: 3610-3618 [Abstract] [Full Text]  
  • Postigo, A. A., Dean, D. C. (1999). Independent Repressor Domains in ZEB Regulate Muscle and T-Cell Differentiation. Mol. Cell. Biol. 19: 7961-7971 [Abstract] [Full Text]  
  • Furusawa, T., Moribe, H., Kondoh, H., Higashi, Y. (1999). Identification of CtBP1 and CtBP2 as Corepressors of Zinc Finger-Homeodomain Factor delta EF1. Mol. Cell. Biol. 19: 8581-8590 [Abstract] [Full Text]  
  • Postigo, A. A., Ward, E., Skeath, J. B., Dean, D. C. (1999). zfh-1, the Drosophila Homologue of ZEB, Is a Transcriptional Repressor That Regulates Somatic Myogenesis. Mol. Cell. Biol. 19: 7255-7263 [Abstract] [Full Text]  
  • Brabletz, T., Jung, A., Hlubek, F., Lohberg, C., Meiler, J., Suchy, U., Kirchner, T. (1999). Negative regulation of CD4 expression in T cells by the transcriptional repressor ZEB. Int Immunol 11: 1701-1708 [Abstract] [Full Text]  
  • Postigo, A. A., Dean, D. C. (1999). ZEB represses transcription through interaction with the corepressor CtBP. Proc. Natl. Acad. Sci. USA 96: 6683-6688 [Abstract] [Full Text]  
  • Johnson, A. D., Owens, G. K. (1999). Differential activation of the SMalpha A promoter in smooth vs. skeletal muscle cells by bHLH factors. Am. J. Physiol. Cell Physiol. 276: C1420-C1431 [Abstract] [Full Text]  
  • Chamberlain, E. M., Sanders, M. M. (1999). Identification of the Novel Player delta EF1 in Estrogen Transcriptional Cascades. Mol. Cell. Biol. 19: 3600-3606 [Abstract] [Full Text]  
  • Gregoire, J.-M., Romeo, P.-H. (1999). T-cell Expression of the Human GATA-3 Gene Is Regulated by a Non-lineage-specific Silencer. J. Biol. Chem. 274: 6567-6578 [Abstract] [Full Text]  
  • NIKOLAJCZYK, B.S., DANG, W., SEN, R. (1999). Mechanisms of {micro} Enhancer Regulation in B Lymphocytes. Cold Spring Harb Symp Quant Biol 64: 99-108 [Abstract]  
  • Wang, Z., Goldstein, A., Zong, R.-T., Lin, D., Neufeld, E. J., Scheuermann, R. H., Tucker, P. W. (1999). Cux/CDP Homeoprotein Is a Component of NF-µNR and Represses the Immunoglobulin Heavy Chain Intronic Enhancer by Antagonizing the Bright Transcription Activator. Mol. Cell. Biol. 19: 284-295 [Abstract] [Full Text]  
  • WEBB, C., ZONG, R.-T., LIN, D., WANG, Z., KAPLAN, M., PAULIN, Y., SMITH, E., PROBST, L., BRYANT, J., GOLDSTEIN, A., SCHEUERMANN, R., TUCKER, P. (1999). Differential Regulation of Immunoglobulin Gene Transcription via Nuclear Matrix-associated Regions. Cold Spring Harb Symp Quant Biol 64: 109-118 [Abstract]  
  • Lovmand, J., Sorensen, A. B., Schmidt, J., Ostergaard, M., Luz, A., Pedersen, F. S. (1998). B-Cell Lymphoma Induction by Akv Murine Leukemia Viruses Harboring One or Both Copies of the Tandem Repeat in the U3 Enhancer. J. Virol. 72: 5745-5756 [Abstract] [Full Text]  
  • Kraner, S. D., Rich, M. M., Kallen, R. G., Barchi, R. L. (1998). Two E-Boxes Are the Focal Point of Muscle-specific Skeletal Muscle Type 1 Na+ Channel Gene Expression. J. Biol. Chem. 273: 11327-11334 [Abstract] [Full Text]  
  • Yasui, D. H., Genetta, T., Kadesch, T., Williams, T. M., Swain, S. L., Tsui, L. V., Huber, B. T. (1998). Transcriptional Repression of the IL-2 Gene in Th Cells by ZEB. J. Immunol. 160: 4433-4440 [Abstract] [Full Text]  
  • Dang, W., Sun, X.-h., Sen, R. (1998). ETS-Mediated Cooperation between Basic Helix-Loop-Helix Motifs of the Immunoglobulin µ Heavy-Chain Gene Enhancer. Mol. Cell. Biol. 18: 1477-1488 [Abstract] [Full Text]  
  • Ikeda, K., Halle, J.-P., Stelzer, G., Meisterernst, M., Kawakami, K. (1998). Involvement of Negative Cofactor NC2 in Active Repression by Zinc Finger-Homeodomain Transcription Factor AREB6. Mol. Cell. Biol. 18: 10-18 [Abstract] [Full Text]  
  • Takagi, T, Moribe, H, Kondoh, H, Higashi, Y (1998). DeltaEF1, a zinc finger and homeodomain transcription factor, is required for skeleton patterning in multiple lineages. Development 125: 21-31 [Abstract]  
  • Lupo, A., Costanzo, P., Medugno, L., Romeo, I., Salvatore, F., Izzo, P. (1997). Negative Regulation of the Mouse Aldolase A Gene. A CELL CYCLE-DEPENDENT DNA BINDING ACTIVITY FUNCTIONS AS A SILENCER OF GENE TRANSCRIPTION. J. Biol. Chem. 272: 31641-31647 [Abstract] [Full Text]  
  • Higashi, Y., Moribe, H., Takagi, T., Sekido, R., Kawakami, K., Kikutani, H., Kondoh, H. (1997). Impairment of T Cell Development in {delta}EF1 Mutant Mice. JEM 185: 1467-1480 [Abstract] [Full Text]  
  • Roberts, J. L., Lauzurica, P., Krangel, M. S. (1997). Developmental Regulation of VDJ Recombination By the Core Fragment of the T Cell Receptor {alpha} Enhancer. JEM 185: 131-140 [Abstract] [Full Text]  
  • Garami, M., Gardner, D. G. (1996). An E-Box Motif Conveys Inhibitory Activity on the Atrial Natriuretic Peptide Gene. Hypertension 28: 315-319 [Abstract] [Full Text]  
  • Shin, E. K., Tevosian, S. G., Yee, A. S. (1996). The N-terminal Region of E2F-1 Is Required for Transcriptional Activation of a New Class of Target Promoter. J. Biol. Chem. 271: 12261-12268 [Abstract] [Full Text]  
  • Gray, S, Levine, M (1996). Short-range transcriptional repressors mediate both quenching and direct repression within complex loci in Drosophila.. Genes Dev. 10: 700-710 [Abstract]  
  • Desbarats, L, Gaubatz, S, Eilers, M (1996). Discrimination between different E-box-binding proteins at an endogenous target gene of c-myc.. Genes Dev. 10: 447-460 [Abstract]  
  • Herrscher, R F, Kaplan, M H, Lelsz, D L, Das, C, Scheuermann, R, Tucker, P W (1995). The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family.. Genes Dev. 9: 3067-3082 [Abstract]  
  • Engeland, K., Andrews, N. C., Mathey-Prevot, B. (1995). Multiple Proteins Interact with the Nuclear Inhibitory Protein Repressor Element in the Human Interleukin-3 Promoter. J. Biol. Chem. 270: 24572-24579 [Abstract] [Full Text]  
  • Apone, S., Hauschka, S. D. (1995). Muscle Gene E-box Control Elements. J. Biol. Chem. 270: 21420-21427 [Abstract] [Full Text]  
  • Berry, F. B., Miura, Y., Mihara, K., Kaspar, P., Sakata, N., Hashimoto-Tamaoki, T., Tamaoki, T. (2001). Positive and Negative Regulation of Myogenic Differentiation of C2C12 Cells by Isoforms of the Multiple Homeodomain Zinc Finger Transcription Factor ATBF1. J. Biol. Chem. 276: 25057-25065 [Abstract] [Full Text]  
  • Cioffi, C. C., Middleton, D. L., Wilson, M. R., Miller, N. W., Clem, L. W., Warr, G. W. (2001). An IgH Enhancer That Drives Transcription through Basic Helix-Loop-Helix and Oct Transcription Factor Binding Motifs. FUNCTIONAL ANALYSIS OF THE E{micro}3' ENHANCER OF THE CATFISH. J. Biol. Chem. 276: 27825-27830 [Abstract] [Full Text]  
  • Kaplan, M. H., Zong, R.-T., Herrscher, R. F., Scheuermann, R. H., Tucker, P. W. (2001). Transcriptional Activation by a Matrix Associating Region-binding Protein. CONTEXTUAL REQUIREMENTS FOR THE FUNCTION OF BRIGHT. J. Biol. Chem. 276: 21325-21330 [Abstract] [Full Text]  
  • Sun, C.-T., Lo, W.-Y., Wang, I.-H., Lo, Y.-H., Shiou, S.-R., Lai, C.-K., Ting, L.-P. (2001). Transcription Repression of Human Hepatitis B Virus Genes by Negative Regulatory Element-binding Protein/SON. J. Biol. Chem. 276: 24059-24067 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»