This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lecka-Czernik, B. Articles by Goldstein, S. Search for Related Content PubMed PubMed Citation Articles by Lecka-Czernik, B. Articles by Goldstein, S.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 01 1995, 120-128, Vol 15, No. 1
Copyright © 1995, American Society for Microbiology

An overexpressed gene transcript in senescent and quiescent human fibroblasts encoding a novel protein in the epidermal growth factor- like repeat family stimulates DNA synthesis

B Lecka-Czernik, CK Lumpkin Jr and S Goldstein
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock.

We carried out subtractive enrichment of a cDNA library derived from mRNA of senescent human diploid fibroblasts (HDF) established from a subject with Werner syndrome of premature aging. By differential screening, we isolated an overexpressed cDNA sequence (S1-5) that codes for a novel protein containing epidermal growth factor (EGF)-like domains which match the EGF-like consensus sequences within several known extracellular proteins that play a role in cell growth, development, and cell signalling. S1-5 mRNA is overexpressed in Werner syndrome and senescent normal HDF, is induced by growth arrest of young normal cells, but is significantly decreased by high serum, conditions which promote cellular proliferation. Paradoxically, microinjection into young HDF of two different lengths of S1-5 mRNA, containing different putative AUG translational start sites, consistently stimulated rather than inhibited DNA synthesis by an apparent autocrine/paracrine mechanism. Thus, the S1-5 gene product may represent a negative and/or positive factor whose ultimate activity is modulated by the cell environment as occurs with other members of EGF- like family.

This article has been cited by other articles:

• McLaughlin, P. J., Bakall, B., Choi, J., Liu, Z., Sasaki, T., Davis, E. C., Marmorstein, A. D., Marmorstein, L. Y. (2007). Lack of fibulin-3 causes early aging and herniation, but not macular degeneration in mice. Hum Mol Genet 16: 3059-3070 [Abstract] [Full Text]  
• Marmorstein, L. Y., McLaughlin, P. J., Peachey, N. S., Sasaki, T., Marmorstein, A. D. (2007). Formation and progression of sub-retinal pigment epithelium deposits in Efemp1 mutation knock-in mice: a model for the early pathogenic course of macular degeneration. Hum Mol Genet 16: 2423-2432 [Abstract] [Full Text]  
• Kobayashi, N., Kostka, G., Garbe, J. H. O., Keene, D. R., Bachinger, H. P., Hanisch, F.-G., Markova, D., Tsuda, T., Timpl, R., Chu, M.-L., Sasaki, T. (2007). A Comparative Analysis of the Fibulin Protein Family: BIOCHEMICAL CHARACTERIZATION, BINDING INTERACTIONS, AND TISSUE LOCALIZATION. J. Biol. Chem. 282: 11805-11816 [Abstract] [Full Text]  
• El-Hallous, E., Sasaki, T., Hubmacher, D., Getie, M., Tiedemann, K., Brinckmann, J., Batge, B., Davis, E. C., Reinhardt, D. P. (2007). Fibrillin-1 Interactions with Fibulins Depend on the First Hybrid Domain and Provide an Adaptor Function to Tropoelastin. J. Biol. Chem. 282: 8935-8946 [Abstract] [Full Text]  
• Hesselson, D., Kimble, J. (2006). Growth control by EGF repeats of the C. elegans Fibulin-1C isoform. J. Cell Biol. 175: 217-223 [Abstract] [Full Text]  
• Albig, A. R., Neil, J. R., Schiemann, W. P. (2006). Fibulins 3 and 5 Antagonize Tumor Angiogenesis In vivo.. Cancer Res. 66: 2621-2629 [Abstract] [Full Text]  
• Muriel, J. M., Dong, C., Hutter, H., Vogel, B. E. (2005). Fibulin-1C and Fibulin-1D splice variants have distinct functions and assemble in a hemicentin-dependent manner. Development 132: 4223-4234 [Abstract] [Full Text]  
• Blackburn, J., Tarttelin, E. E., Gregory-Evans, C. Y., Moosajee, M., Gregory-Evans, K. (2003). Transcriptional Regulation and Expression of the Dominant Drusen Gene FBLN3 (EFEMP1) in Mammalian Retina. IOVS 44: 4613-4621 [Abstract] [Full Text]  
• Marmorstein, L. Y., Munier, F. L., Arsenijevic, Y., Schorderet, D. F., McLaughlin, P. J., Chung, D., Traboulsi, E., Marmorstein, A. D. (2002). Aberrant accumulation of EFEMP1 underlies drusen formation in Malattia Leventinese and age-related macular degeneration. Proc. Natl. Acad. Sci. USA 99: 13067-13072 [Abstract] [Full Text]  
• Schiemann, W. P., Blobe, G. C., Kalume, D. E., Pandey, A., Lodish, H. F. (2002). Context-specific Effects of Fibulin-5 (DANCE/EVEC) on Cell Proliferation, Motility, and Invasion. FIBULIN-5 IS INDUCED BY TRANSFORMING GROWTH FACTOR-beta AND AFFECTS PROTEIN KINASE CASCADES. J. Biol. Chem. 277: 27367-27377 [Abstract] [Full Text]  
• Werling, U., Schorle, H. (2002). Transcription Factor Gene AP-2{gamma} Essential for Early Murine Development. Mol. Cell. Biol. 22: 3149-3156 [Abstract] [Full Text]  
• Jean, J.-C., Eruchalu, I., Cao, Y. X., Joyce-Brady, M. (2002). DANCE in developing and injured lung. Am. J. Physiol. Lung Cell. Mol. Physiol. 282: L75-L82 [Abstract] [Full Text]  
• Chakravortty, D., Kato, Y., Sugiyama, T., Koide, N., Mu, M. M., Yoshida, T., Yokochi, T. (2001). Inhibition of p38 Mitogen-Activated Protein Kinase Augments Lipopolysaccharide-Induced Cell Proliferation in CD14-Expressing Chinese Hamster Ovary Cells. Infect. Immun. 69: 931-936 [Abstract] [Full Text]  
• Nakamura, T., Ruiz-Lozano, P., Lindner, V., Yabe, D., Taniwaki, M., Furukawa, Y., Kobuke, K., Tashiro, K., Lu, Z., Andon, N. L., Schaub, R., Matsumori, A., Sasayama, S., Chien, K. R., Honjo, T. (1999). DANCE, a Novel Secreted RGD Protein Expressed in Developing, Atherosclerotic, and Balloon-injured Arteries. J. Biol. Chem. 274: 22476-22483 [Abstract] [Full Text]  
• Heine, H., Delude, R. L., Monks, B. G., Espevik, T., Golenbock, D. T. (1999). Bacterial Lipopolysaccharide Induces Expression of the Stress Response Genes hop and H411. J. Biol. Chem. 274: 21049-21055 [Abstract] [Full Text]  
• Kowal, R. C., Richardson, J. A., Miano, J. M., Olson, E. N. (1999). EVEC, a Novel Epidermal Growth Factor–Like Repeat-Containing Protein Upregulated in Embryonic and Diseased Adult Vasculature. Circ. Res. 84: 1166-1176 [Abstract] [Full Text]  
• Pfisterer, P., Ehlermann, J., Hegen, M., Schorle, H. (2002). A Subtractive Gene Expression Screen Suggests a Role of Transcription Factor AP-2alpha in Control of Proliferation and Differentiation. J. Biol. Chem. 277: 6637-6644 [Abstract] [Full Text]