This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Horikoshi, N. Articles by Shenk, T. Search for Related Content PubMed PubMed Citation Articles by Horikoshi, N. Articles by Shenk, T.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Jan 1995, 227-234, Vol 15, No. 1
Copyright © 1995, American Society for Microbiology

Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, relieving p53- mediated transcriptional repression

N Horikoshi, A Usheva, J Chen, AJ Levine, R Weinmann and T Shenk
Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, New Jersey 08544-1014.

The tumor suppressor gene product p53 can activate and repress transcription. Both transcriptional activation and repression are thought to involve the direct interaction of p53 with the basal transcriptional machinery. Previous work has demonstrated an in vitro interaction between p53 and the TATA-binding protein that requires amino acids 20 to 57 of p53 and amino acids 220 to 271 of the TATA- binding protein. The present results show that a 75-amino-acid segment from the carboxy terminus of p53 also can bind to the TATA-binding protein in vitro, and this interaction requires amino acids 217 to 268 of the TATA-binding protein, essentially the same domain that is required for interaction with the amino-terminal domain of p53. A carboxy-terminal segment of p53 can mediate repression when bound to DNA as a GAL4-p53 fusion protein. The amino- and carboxy-terminal p53 interactions occur within the domain on the TATA-binding protein to which the adenovirus 13S E1A oncoprotein has previously been shown to bind. The 13S E1A oncoprotein can dissociate the complex formed between the carboxy-terminal domain of p53 and the TATA-binding protein and relieve p53-mediated transcriptional repression. These results demonstrate that two independent domains of p53 can potentially interact with the TATA-binding protein, and they define a mechanism-- relief of repression--by which the 13S E1A oncoprotein can activate transcription through the TATA motif.

This article has been cited by other articles:

• O'Farrell, T. J., Ghosh, P., Dobashi, N., Sasaki, C. Y., Longo, D. L. (2004). Comparison of the Effect of Mutant and Wild-Type p53 on Global Gene Expression. Cancer Res. 64: 8199-8207 [Abstract] [Full Text]  
• Zheng, G., Yang, Y.-C. (2004). ZNF76, a Novel Transcriptional Repressor Targeting TATA-binding Protein, Is Modulated by Sumoylation. J. Biol. Chem. 279: 42410-42421 [Abstract] [Full Text]  
• Stebbins, J. L., Triezenberg, S. J. (2004). Identification, Mutational Analysis, and Coactivator Requirements of Two Distinct Transcriptional Activation Domains of the Saccharomyces cerevisiae Hap4 Protein. Eukaryot Cell 3: 339-347 [Abstract] [Full Text]  
• Schwartzenberg-Bar-Yoseph, F., Armoni, M., Karnieli, E. (2004). The Tumor Suppressor p53 Down-Regulates Glucose Transporters GLUT1 and GLUT4 Gene Expression. Cancer Res. 64: 2627-2633 [Abstract] [Full Text]  
• Yun, J., Chae, H.-D., Choi, T.-S., Kim, E.-H., Bang, Y.-J., Chung, J., Choi, K.-S., Mantovani, R., Shin, D. Y. (2003). Cdk2-dependent Phosphorylation of the NF-Y Transcription Factor and Its Involvement in the p53-p21 Signaling Pathway. J. Biol. Chem. 278: 36966-36972 [Abstract] [Full Text]  
• Ohiro, Y., Usheva, A., Kobayashi, S., Duffy, S. L., Nantz, R., Gius, D., Horikoshi, N. (2003). Inhibition of Stress-Inducible Kinase Pathways by Tumorigenic Mutant p53. Mol. Cell. Biol. 23: 322-334 [Abstract] [Full Text]  
• Serber, Z., Lai, H. C., Yang, A., Ou, H. D., Sigal, M. S., Kelly, A. E., Darimont, B. D., Duijf, P. H. G., van Bokhoven, H., McKeon, F., Dotsch, V. (2002). A C-Terminal Inhibitory Domain Controls the Activity of p63 by an Intramolecular Mechanism. Mol. Cell. Biol. 22: 8601-8611 [Abstract] [Full Text]  
• Mauser, A., Saito, S.'i., Appella, E., Anderson, C. W., Seaman, W. T., Kenney, S. (2002). The Epstein-Barr Virus Immediate-Early Protein BZLF1 Regulates p53 Function through Multiple Mechanisms. J. Virol. 76: 12503-12512 [Abstract] [Full Text]  
• Yang, Y., McKerlie, C., Borenstein, S. H., Lu, Z., Schito, M., Chamberlain, J. W., Buchwald, M. (2002). Transgenic Expression in Mouse Lung Reveals Distinct Biological Roles for the Adenovirus Type 5 E1A 243- and 289-Amino-Acid Proteins. J. Virol. 76: 8910-8919 [Abstract] [Full Text]  
• Gu, L., Findley, H. W., Zhou, M. (2002). MDM2 induces NF-kappa B/p65 expression transcriptionally through Sp1-binding sites: a novel, p53-independent role of MDM2 in doxorubicin resistance in acute lymphoblastic leukemia. Blood 99: 3367-3375 [Abstract] [Full Text]  
• Yan, C., Wang, H., Boyd, D. D. (2002). ATF3 Represses 72-kDa Type IV Collagenase (MMP-2) Expression by Antagonizing p53-dependent trans-Activation of the Collagenase Promoter. J. Biol. Chem. 277: 10804-10812 [Abstract] [Full Text]  
• Angelo, L. S., Talpaz, M., Kurzrock, R. (2002). Autocrine Interleukin-6 Production in Renal Cell Carcinoma: Evidence for the Involvement of p53. Cancer Res. 62: 932-940 [Abstract] [Full Text]  
• Shenk, J. L., Fisher, C. J., Chen, S.-Y., Zhou, X.-F., Tillman, K., Shemshedini, L. (2001). p53 Represses Androgen-induced Transactivation of Prostate-specific Antigen by Disrupting hAR Amino- to Carboxyl-terminal Interaction. J. Biol. Chem. 276: 38472-38479 [Abstract] [Full Text]  
• Sampath, J., Sun, D., Kidd, V. J., Grenet, J., Gandhi, A., Shapiro, L. H., Wang, Q., Zambetti, G. P., Schuetz, J. D. (2001). Mutant p53 Cooperates with ETS and Selectively Up-regulates Human MDR1 Not MRP1. J. Biol. Chem. 276: 39359-39367 [Abstract] [Full Text]  
• Bai, L., Merchant, J. L. (2001). ZBP-89 Promotes Growth Arrest through Stabilization of p53. Mol. Cell. Biol. 21: 4670-4683 [Abstract] [Full Text]  
• Almog, N., Milyavsky, M., Stambolsky, P., Falcovitz, A., Goldfinger, N., Rotter, V. (2001). The role of the C' terminus of murine p53 in the p53/mdm-2 regulatory loop. Carcinogenesis 22: 779-785 [Abstract] [Full Text]  
• Lackinger, D., Eichhorn, U., Kaina, B. (2001). Effect of ultraviolet light, methyl methanesulfonate and ionizing radiation on the genotoxic response and apoptosis of mouse fibroblasts lacking c-Fos, p53 or both. Mutagenesis 16: 233-241 [Abstract] [Full Text]  
• Zhai, W., Comai, L. (2000). Repression of RNA Polymerase I Transcription by the Tumor Suppressor p53. Mol. Cell. Biol. 20: 5930-5938 [Abstract] [Full Text]  
• Mortensen, K., Skouv, J., Hougaard, D. M., Larsson, L.-I. (1999). Endogenous Endothelial Cell Nitric-oxide Synthase Modulates Apoptosis in Cultured Breast Cancer Cells and Is Transcriptionally Regulated by p53. J. Biol. Chem. 274: 37679-37684 [Abstract] [Full Text]  
• Yamano, S., Tokino, T., Yasuda, M., Kaneuchi, M., Takahashi, M., Niitsu, Y., Fujinaga, K., Yamashita, T. (1999). Induction of Transformation and p53-Dependent Apoptosis by Adenovirus Type 5 E4orf6/7 cDNA. J. Virol. 73: 10095-10103 [Abstract] [Full Text]  
• Yun, J., Chae, H.-D., Choy, H. E., Chung, J., Yoo, H.-S., Han, M.-H., Shin, D. Y. (1999). p53 Negatively Regulates cdc2 Transcription via the CCAAT-binding NF-Y Transcription Factor. J. Biol. Chem. 274: 29677-29682 [Abstract] [Full Text]  
• Blander, G., Kipnis, J., Leal, J. F. M., Yu, C.-E., Schellenberg, G. D., Oren, M. (1999). Physical and Functional Interaction between p53 and the Werner's Syndrome Protein. J. Biol. Chem. 274: 29463-29469 [Abstract] [Full Text]  
• Murphy, M., Ahn, J., Walker, K. K., Hoffman, W. H., Evans, R. M., Levine, A. J., George, D. L. (1999). Transcriptional repression by wild-type p53 utilizes histone deacetylases, mediated by interaction with mSin3a. Genes Dev. 13: 2490-2501 [Abstract] [Full Text]  
• Devireddy, L. R., Jones, C. J. (1999). Activation of Caspases and p53 by Bovine Herpesvirus 1 Infection Results in Programmed Cell Death and Efficient Virus Release. J. Virol. 73: 3778-3788 [Abstract] [Full Text]  
• Shirra, M. K., Arndt, K. M. (1999). Evidence for the Involvement of the Glc7-Reg1 Phosphatase and the Snf1-Snf4 Kinase in the Regulation of INO1 Transcription in Saccharomyces cerevisiae. Genetics 152: 73-87 [Abstract] [Full Text]  
• Tung, S.-F., Chuang, J.-Y., Lin, C.-T., Lai, M.-Y., Wu, C.-W., Lin, Y.-S. (1999). Inhibition of hTAFII32-binding Implicated in the Transcriptional Repression by Central Regions of Mutant p53 Proteins. J. Biol. Chem. 274: 7748-7755 [Abstract] [Full Text]  
• Zhu, J., Zhou, W., Jiang, J., Chen, X. (1998). Identification of a Novel p53 Functional Domain That Is Necessary for Mediating Apoptosis. J. Biol. Chem. 273: 13030-13036 [Abstract] [Full Text]  
• Chien, Y.-t., Helmann, J. D., Zinder, S. H. (1998). Interactions between the Promoter Regions of Nitrogenase Structural Genes (nifHDK2) and DNA-Binding Proteins from N2- and Ammonium-Grown Cells of the Archaeon Methanosarcina barkeri 227. J. Bacteriol. 180: 2723-2728 [Abstract] [Full Text]  
• Li, Z., Yu, A., Weiner, A. M. (1998). Adenovirus Type 12-Induced Fragility of the Human RNU2 Locus Requires p53 Function. J. Virol. 72: 4183-4191 [Abstract] [Full Text]  
• Gopalkrishnan, R. V., Lam, E. W.-F., Kedinger, C. (1998). The p53 Tumor Suppressor Inhibits Transcription of the TATA-less Mouse DP1 Promoter. J. Biol. Chem. 273: 10972-10978 [Abstract] [Full Text]  
• Wing, B. A., Johnson, R. A., Huang, E.-S. (1998). Identification of Positive and Negative Regulatory Regions Involved in Regulating Expression of the Human Cytomegalovirus UL94 Late Promoter: Role of IE2-86 and Cellular p53 in Mediating Negative Regulatory Function. J. Virol. 72: 1814-1825 [Abstract] [Full Text]  
• Sablina, A., Ilyinskaya, G., Rubtsova, S., Agapova, L., Chumakov, P., Kopnin, B. (1998). Activation of p53-mediated cell cycle checkpoint in response to micronuclei formation. J. Cell Sci. 111: 977-984 [Abstract]  
• Nagaich, A. K., Appella, E., Harrington, R. E. (1997). DNA Bending Is Essential for the Site-specific Recognition of DNA Response Elements by the DNA Binding Domain of the Tumor Suppressor Protein p53. J. Biol. Chem. 272: 14842-14849 [Abstract] [Full Text]  
• Maiyar, A. C., Phu, P. T., Huang, A. J., Firestone, G. L. (1997). Repression of Glucocorticoid Receptor Transactivation and DNA Binding of a Glucocorticoid Response Element within the Serum/Glucocorticoid-Inducible Protein Kinase (sgk) Gene Promoter by the p53 Tumor Suppressor Protein. Mol. Endocrinol. 11: 312-329 [Abstract] [Full Text]  
• Nevels, M., Rubenwolf, S., Spruss, T., Wolf, H., Dobner, T. (1997). The adenovirus E4orf6 protein can promote E1A/E1B-induced focus formation by interfering with p53 tumor suppressor function. Proc. Natl. Acad. Sci. USA 94: 1206-1211 [Abstract] [Full Text]  
• Hayashi, S., Rubinfeld, B., Souza, B., Polakis, P., Wieschaus, E., Levine, A. J. (1997). A Drosophila homolog of the tumor suppressor gene adenomatous polyposis coli down-regulates beta -catenin but its zygotic expression is not essential for the regulation of Armadillo. Proc. Natl. Acad. Sci. USA 94: 242-247 [Abstract] [Full Text]  
• Chen, X, Ko, L J, Jayaraman, L, Prives, C (1996). p53 levels, functional domains, and DNA damage determine the extent of the apoptotic response of tumor cells.. Genes Dev. 10: 2438-2451 [Abstract]  
• Maiyar, A. C., Huang, A. J., Phu, P. T., Cha, H. H., Firestone, G. L. (1996). p53 Stimulates Promoter Activity of the sgk Serum/Glucocorticoid-inducible Serine/Threonine Protein Kinase Gene in Rodent Mammary Epithelial Cells. J. Biol. Chem. 271: 12414-12422 [Abstract] [Full Text]  
• Ko, L J, Prives, C (1996). p53: puzzle and paradigm.. Genes Dev. 10: 1054-1072  
• Tsai, H.-L., Kou, G.-H., Chen, S.-C., Wu, C.-W., Lin, Y.-S. (1996). Human Cytomegalovirus Immediate-Early Protein IE2 Tethers a Transcriptional Repression Domain to p53. J. Biol. Chem. 271: 3534-3540 [Abstract] [Full Text]  
• Chang, J., Kim, D.-H., Lee, S. W., Choi, K. Y., Sung, Y. C. (1995). Transactivation Ability of p53 Transcriptional Activation Domain Is Directly Related to the Binding Affinity to TATA-binding Protein. J. Biol. Chem. 270: 25014-25019 [Abstract] [Full Text]  
• Chuang, J.-Y., Lin, C.-T., Wu, C.-W., Lin, Y.-S. (1995). A Movable and Regulable Inactivation Function within the Central Region of a Temperature-sensitive p53 Mutant. J. Biol. Chem. 270: 23899-23902 [Abstract] [Full Text]  
• Ogden, S. K., Lee, K. C., Barton, M. C. (2000). Hepatitis B Viral Transactivator HBx Alleviates p53-mediated Repression of alpha -Fetoprotein Gene Expression. J. Biol. Chem. 275: 27806-27814 [Abstract] [Full Text]  
• Lu, W., Peterson, R., Dasgupta, A., Scovell, W. M. (2000). Influence of HMG-1 and Adenovirus Oncoprotein E1A on Early Stages of Transcriptional Preinitiation Complex Assembly. J. Biol. Chem. 275: 35006-35012 [Abstract] [Full Text]  
• Pombo, P. M. G., Barettino, D., Espliguero, G., Metsis, M., Iglesias, T., Rodriguez-Pena, A. (2000). Transcriptional Repression of Neurotrophin Receptor trkB by Thyroid Hormone in the Developing Rat Brain. J. Biol. Chem. 275: 37510-37517 [Abstract] [Full Text]  
• D'Souza, S., Xin, H., Walter, S., Choubey, D. (2001). The Gene Encoding p202, an Interferon-inducible Negative Regulator of the p53 Tumor Suppressor, Is a Target of p53-mediated Transcriptional Repression. J. Biol. Chem. 276: 298-305 [Abstract] [Full Text]  
• Hong, T.-M., Chen, J. J. W., Peck, K., Yang, P.-C., Wu, C.-W. (2001). p53 Amino Acids 339-346 Represent the Minimal p53 Repression Domain. J. Biol. Chem. 276: 1510-1515 [Abstract] [Full Text]  
• Wiederschain, D., Gu, J., Yuan, Z.-M. (2001). Evidence for a Distinct Inhibitory Factor in the Regulation of p53 Functional Activity. J. Biol. Chem. 276: 27999-28005 [Abstract] [Full Text]