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Mol. Cell. Biol., Jan 1995, 505-516, Vol 15, No. 1
Copyright © 1995, American Society for Microbiology

Modulation of cellular thermoresistance and actin filament stability accompanies phosphorylation-induced changes in the oligomeric structure of heat shock protein 27

JN Lavoie, H Lambert, E Hickey, LA Weber and J Landry
Centre de recherche en cancerologie de l'Universite Laval, L'Hotel-Dieu de Quebec, Canada.

Phosphorylation of heat shock protein 27 (HSP27) can modulate actin filament dynamics in response to growth factors. During heat shock, HSP27 is phosphorylated at the same sites and by the same protein kinase as during mitogenic stimulation. This suggests that the same function of the protein may be activated during growth factor stimulation and the stress response. To determine the role of HSP27 phosphorylation in the heat shock response, several stable Chinese hamster cell lines that constitutively express various levels of the wild-type HSP27 (HU27 cells) or a nonphosphorylatable form of human HSP27 (HU27pm3 cells) were developed. In contrast to HU27 cells, which showed increased survival after heat shock, HU27pm3 cells showed only slightly enhanced survival. Evidence is presented that stabilization of microfilaments is a major target of the protective function of HSP27. In the HU27pm3 cells, the microfilaments were thermosensitized compared with those in the control cells, whereas wild-type HSP27 caused an increased stability of these structures in HU27 cells. HU27 but not HU27pm3 cells were highly resistant to cytochalasin D treatment compared with control cells. Moreover, in cells treated with cytochalasin D, wild-type HSP27 but not the phosphorylated form of HSP27 accelerated the reappearance of actin filaments. The mutations in human HSP27 had no effect on heat shock-induced change in solubility and cellular localization of the protein, indicating that phosphorylation was not involved in these processes. However, induction of HSP27 phosphorylation by stressing agents or mitogens caused a reduction in the multimeric size of the wild-type protein, an effect which was not observed with the mutant protein. We propose that early during stress, phosphorylation-induced conformational changes in the HSP27 oligomers regulate the activity of the protein at the level of microfilament dynamics, resulting in both enhanced stability and accelerated recovery of the filaments. The level of protection provided by HSP27 during heat shock may thus represent the contribution of better maintenance of actin filament integrity to overall cell survival.


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  • Gabai, V. L., Sherman, M. Y. (2002). Molecular Biology of Thermoregulation: Invited Review: Interplay between molecular chaperones and signaling pathways in survival of heat shock. J. Appl. Physiol. 92: 1743-1748 [Abstract] [Full Text]  
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  • Deschesnes, R. G., Huot, J., Valerie, K., Landry, J. (2001). Involvement of p38 in Apoptosis-associated Membrane Blebbing and Nuclear Condensation. Mol. Biol. Cell 12: 1569-1582 [Abstract] [Full Text]  
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  • Lavoie, J. N., Champagne, C., Gingras, M.-C., Robert, A. (2000). Adenovirus E4 Open Reading Frame 4-Induced Apoptosis Involves Dysregulation of Src Family Kinases. JCB 150: 1037-1056 [Abstract] [Full Text]  
  • Meloche, S., Landry, J., Huot, J., Houle, F., Marceau, F., Giasson, E. (2000). p38 MAP kinase pathway regulates angiotensin II-induced contraction of rat vascular smooth muscle. Am. J. Physiol. Heart Circ. Physiol. 279: H741-H751 [Abstract] [Full Text]  
  • Tezel, G., Wax, M. B. (2000). The Mechanisms of hsp27 Antibody-Mediated Apoptosis in Retinal Neuronal Cells. J. Neurosci. 20: 3552-3562 [Abstract] [Full Text]  
  • Dana, A., Skarli, M., Papakrivopoulou, J., Yellon, D. M. (2000). Adenosine A1 Receptor Induced Delayed Preconditioning in Rabbits : Induction of p38 Mitogen-Activated Protein Kinase Activation and Hsp27 Phosphorylation via a Tyrosine Kinase- and Protein Kinase C-Dependent Mechanism. Circ. Res. 86: 989-997 [Abstract] [Full Text]  
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  • SMOYER, W. E., RANSOM, R., HARRIS, R. C., WELSH, M. J., LUTSCH, G., BENNDORF, R. (2000). Ischemic Acute Renal Failure Induces Differential Expression of Small Heat Shock Proteins. J. Am. Soc. Nephrol. 11: 211-221 [Abstract] [Full Text]  
  • Bova, M. P., Mchaourab, H. S., Han, Y., Fung, B. K.-K. (2000). Subunit Exchange of Small Heat Shock Proteins. ANALYSIS OF OLIGOMER FORMATION OF alpha A-CRYSTALLIN AND Hsp27 BY FLUORESCENCE RESONANCE ENERGY TRANSFER AND SITE-DIRECTED TRUNCATIONS. J. Biol. Chem. 275: 1035-1042 [Abstract] [Full Text]  
  • Sugiyama, Y., Suzuki, A., Kishikawa, M., Akutsu, R., Hirose, T., Waye, M. M. Y., Tsui, S. K. W., Yoshida, S., Ohno, S. (2000). Muscle Develops a Specific Form of Small Heat Shock Protein Complex Composed of MKBP/HSPB2 and HSPB3 during Myogenic Differentiation. J. Biol. Chem. 275: 1095-1104 [Abstract] [Full Text]  
  • Dorion, S., Berube, J., Huot, J., Landry, J. (1999). A Short Lived Protein Involved in the Heat Shock Sensing Mechanism Responsible for Stress-activated Protein Kinase 2 (SAPK2/p38) Activation. J. Biol. Chem. 274: 37591-37597 [Abstract] [Full Text]  
  • Schafer, C., Clapp, P., Welsh, M. J., Benndorf, R., Williams, J. A. (1999). HSP27 expression regulates CCK-induced changes of the actin cytoskeleton in CHO-CCK-A cells. Am. J. Physiol. Cell Physiol. 277: C1032-C1043 [Abstract] [Full Text]  
  • Imura, T., Shimohama, S., Sato, M., Nishikawa, H., Madono, K., Akaike, A., Kimura, J. (1999). Differential Expression of Small Heat Shock Proteins in Reactive Astrocytes after Focal Ischemia: Possible Role of beta -Adrenergic Receptor. J. Neurosci. 19: 9768-9779 [Abstract] [Full Text]  
  • Yoshida, N., Kristiansen, A., Liberman, M. C. (1999). Heat Stress and Protection from Permanent Acoustic Injury in Mice. J. Neurosci. 19: 10116-10124 [Abstract] [Full Text]  
  • Wagner, M., Hermanns, I., Bittinger, F., Kirkpatrick, C. J. (1999). Induction of stress proteins in human endothelial cells by heavy metal ions and heat shock. Am. J. Physiol. Lung Cell. Mol. Physiol. 277: L1026-L1033 [Abstract] [Full Text]  
  • Lewis, S. E., Mannion, R. J., White, F. A., Coggeshall, R. E., Beggs, S., Costigan, M., Martin, J. L., Dillmann, W. H., Woolf, C. J. (1999). A Role for HSP27 in Sensory Neuron Survival. J. Neurosci. 19: 8945-8953 [Abstract] [Full Text]  
  • Kasi, V. S., Kuppuswamy, D. (1999). Inhibition of Src Family Kinases by a Combinatorial Action of 5'-AMP and Small Heat Shock Proteins, Identified from the Adult Heart. Mol. Cell. Biol. 19: 6858-6871 [Abstract] [Full Text]  
  • Hedges, J. C., Dechert, M. A., Yamboliev, I. A., Martin, J. L., Hickey, E., Weber, L. A., Gerthoffer, W. T. (1999). A Role for p38MAPK/HSP27 Pathway in Smooth Muscle Cell Migration. J. Biol. Chem. 274: 24211-24219 [Abstract] [Full Text]  
  • van Rijk, A. A. F., de Jong, W. W., Bloemendal, H. (1999). Exon shuffling mimicked in cell culture. Proc. Natl. Acad. Sci. USA 96: 8074-8079 [Abstract] [Full Text]  
  • Rogalla, T., Ehrnsperger, M., Preville, X., Kotlyarov, A., Lutsch, G., Ducasse, C., Paul, C., Wieske, M., Arrigo, A.-P., Buchner, J., Gaestel, M. (1999). Regulation of Hsp27 Oligomerization, Chaperone Function, and Protective Activity against Oxidative Stress/Tumor Necrosis Factor alpha  by Phosphorylation. J. Biol. Chem. 274: 18947-18956 [Abstract] [Full Text]  
  • Heuertz, R. M., Tricomi, S. M., Ezekiel, U. R., Webster, R. O. (1999). C-reactive Protein Inhibits Chemotactic Peptide-induced p38 Mitogen-activated Protein Kinase Activity and Human Neutrophil Movement. J. Biol. Chem. 274: 17968-17974 [Abstract] [Full Text]  
  • Xi, G., Keep, R. F., Hua, Y., Xiang, J., Hoff, J. T., Macdonald, R. L. (1999). Attenuation of Thrombin-Induced Brain Edema by Cerebral Thrombin Preconditioning • Editorial Comment. Stroke 30: 1247-1255 [Abstract] [Full Text]  
  • Ehrnsperger, M., Lilie, H., Gaestel, M., Buchner, J. (1999). The Dynamics of Hsp25 Quaternary Structure. STRUCTURE AND FUNCTION OF DIFFERENT OLIGOMERIC SPECIES. J. Biol. Chem. 274: 14867-14874 [Abstract] [Full Text]  
  • Beall, A., Bagwell, D., Woodrum, D., Stoming, T. A., Kato, K., Suzuki, A., Rasmussen, H., Brophy, C. M. (1999). The Small Heat Shock-related Protein, HSP20, Is Phosphorylated on Serine 16 during Cyclic Nucleotide-dependent Relaxation. J. Biol. Chem. 274: 11344-11351 [Abstract] [Full Text]  
  • Lambert, H., Charette, S. J., Bernier, A. F., Guimond, A., Landry, J. (1999). HSP27 Multimerization Mediated by Phosphorylation-sensitive Intermolecular Interactions at the Amino Terminus. J. Biol. Chem. 274: 9378-9385 [Abstract] [Full Text]  
  • Evans, S. S., Schleider, D. M., Bowman, L. A., Francis, M. L., Kansas, G. S., Black, J. D. (1999). Dynamic Association of L-Selectin with the Lymphocyte Cytoskeletal Matrix. J. Immunol. 162: 3615-3624 [Abstract] [Full Text]  
  • Brophy, C. M., Dickinson, M., Woodrum, D. (1999). Phosphorylation of the Small Heat Shock-related Protein, HSP20, in Vascular Smooth Muscles Is Associated with Changes in the Macromolecular Associations of HSP20. J. Biol. Chem. 274: 6324-6329 [Abstract] [Full Text]  
  • Loktionova, S. A., Ilyinskaya, O. P., Kabakov, A. E. (1998). Early and delayed tolerance to simulated ischemia in heat-preconditioned endothelial cells: a role for HSP27. Am. J. Physiol. Heart Circ. Physiol. 275: H2147-H2158 [Abstract] [Full Text]  
  • Huot, J., Houle, F., Rousseau, S., Deschesnes, R. G., Shah, G. M., Landry, J. (1998). SAPK2/p38-dependent F-Actin Reorganization Regulates Early Membrane Blebbing during Stress-induced Apoptosis. JCB 143: 1361-1373 [Abstract] [Full Text]  


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