This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Feldherr, C. Articles by Akin, D. Search for Related Content PubMed PubMed Citation Articles by Feldherr, C. Articles by Akin, D.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 12 1995, 7043-7049, Vol 15, No. 12
Copyright © 1995, American Society for Microbiology

Stimulation of nuclear import by simian virus 40-transformed cell extracts is dependent on protein kinase activity

C Feldherr and D Akin
Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville 32610, USA.

We previously reported that both the nuclear import rate of large karyophilic gold particles and the functional size of the pores are significantly greater in simian virus 40-transformed fibroblasts (the SV-T2 cell line) than in nontransformed BALB/c 3T3 cells. In this study, we found that cytosolic fractions obtained from SV-T2 cultures can increase nuclear transport capacity (both import rate and pore size) when microinjected into BALB/c 3T3 cells. The transport-enhancing function of the extracts can be abolished by the protein kinase inhibitors staurosporine and K252a as well as 5'-p- fluorosulfonylbenzoyladenosine and protein phosphatase 2A, which, although less specific, also interfere with kinase activity. Increases in transport capacity of the same magnitude as that produced by the SV- T2 extracts were obtained by microinjecting protein kinase A or C or recombinant mitogen-activated protein kinase. These data provide further support for the interpretation that the enhancer is a protein kinase. From experiments performed with specific kinase inhibitor peptides, it appears likely that protein kinase C is the active factor in the SV-T2 cytosolic fractions; however, this will require further verification. It was also determined, by using gold particles coated with bovine serum albumin conjugated to synthetic nuclear localization signal peptides that lacked phosphorylation sites, that the enhancer affects the transport machinery rather than the activity of the nuclear localization signals.

This article has been cited by other articles:

• Tsuji, T., Sheehy, N., Gautier, V. W., Hayakawa, H., Sawa, H., Hall, W. W. (2007). The Nuclear Import of the Human T Lymphotropic Virus Type I (HTLV-1) Tax Protein Is Carrier- and Energy-independent. J. Biol. Chem. 282: 13875-13883 [Abstract] [Full Text]  
• Lidsky, P. V., Hato, S., Bardina, M. V., Aminev, A. G., Palmenberg, A. C., Sheval, E. V., Polyakov, V. Y., van Kuppeveld, F. J. M., Agol, V. I. (2006). Nucleocytoplasmic Traffic Disorder Induced by Cardioviruses. J. Virol. 80: 2705-2717 [Abstract] [Full Text]  
• Griffis, E. R., Craige, B., Dimaano, C., Ullman, K. S., Powers, M. A. (2004). Distinct Functional Domains within Nucleoporins Nup153 and Nup98 Mediate Transcription-dependent Mobility. Mol. Biol. Cell 15: 1991-2002 [Abstract] [Full Text]  
• Mertani, H. C., Raccurt, M., Abbate, A., Kindblom, J., Tornell, J., Billestrup, N., Usson, Y., Morel, G., Lobie, P. E. (2003). Nuclear Translocation and Retention of Growth Hormone. Endocrinology 144: 3182-3195 [Abstract] [Full Text]  
• Feldherr, C. M., Akin, D., Cohen, R. J. (2002). Regulation of functional nuclear pore size in fibroblasts. J. Cell Sci. 114: 4621-4627 [Abstract] [Full Text]  
• Feldherr, C., Akin, D (1999). Signal-mediated nuclear transport in the amoeba. J. Cell Sci. 112: 2043-2048 [Abstract]  
• Lu, D., Yang, H., Raizada, M. K. (1998). Involvement of p62 Nucleoporin in Angiotensin II-Induced Nuclear Translocation of STAT3 in Brain Neurons. J. Neurosci. 18: 1329-1336 [Abstract] [Full Text]  
• Kehlenbach, R. H., Gerace, L. (2000). Phosphorylation of the Nuclear Transport Machinery Down-regulates Nuclear Protein Import in Vitro. J. Biol. Chem. 275: 17848-17856 [Abstract] [Full Text]