This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fotedar, R.
Right arrow Articles by Fotedar, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fotedar, R.
Right arrow Articles by Fotedar, A.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Feb 1995, 932-942, Vol 15, No. 2
Copyright © 1995, American Society for Microbiology

Activation-induced T-cell death is cell cycle dependent and regulated by cyclin B

R Fotedar, J Flatt, S Gupta, RL Margolis, P Fitzgerald, H Messier and A Fotedar
Institut de Biologie Structurale J.-P. Ebel, Grenoble, France.

Developing thymocytes and some T-cell hybridomas undergo activation- dependent programmed cell death. Although recent studies have identified some critical regulators in programmed cell death, the role of cell cycle regulation in activation-induced cell death in T cells has not been addressed. We demonstrate that synchronized T-cell hybridomas, irrespective of the point in the cell cycle at which they are activated, stop cycling shortly after they reach G2/M. These cells exhibit the diagnostic characteristics of apoptotic cell death. Although p34cdc2 levels are not perturbed after activation of synchronously cycling T cells, cyclin B- and p34cdc2-associated histone H1 kinase activity is persistently elevated. This activation-dependent induction of H1 kinase activity in T cells is associated with a decrease in the phosphotyrosine content of p34cdc2. We also demonstrate that transient inappropriate coexpression of cyclin B with p34cdc2 induces DNA fragmentation in a heterologous cell type. Finally, in T cells, cyclin B-specific antisense oligonucleotides suppress activation- induced cell death but not cell death induced by exposure to dexamethasone. We therefore conclude that a persistent elevation of the level of cyclin B kinase is required for activation-induced programmed T-cell death.


This article has been cited by other articles:

  • Pal, M., Nagy, O., Menesi, D., Udvardy, A., Deak, P. (2007). Structurally related TPR subunits contribute differently to the function of the anaphase-promoting complex in Drosophila melanogaster. J. Cell Sci. 120: 3238-3248 [Abstract] [Full Text]  
  • Curry, C. L., Reed, L. L., Broude, E., Golde, T. E., Miele, L., Foreman, K. E. (2007). Notch inhibition in Kaposi's sarcoma tumor cells leads to mitotic catastrophe through nuclear factor-{kappa}B signaling. Molecular Cancer Therapeutics 6: 1983-1992 [Abstract] [Full Text]  
  • Smith, L., Watson, M. B., O'Kane, S. L., Drew, P. J., Lind, M. J., Cawkwell, L. (2006). The analysis of doxorubicin resistance in human breast cancer cells using antibody microarrays.. Molecular Cancer Therapeutics 5: 2115-2120 [Abstract] [Full Text]  
  • Niida, H., Tsuge, S., Katsuno, Y., Konishi, A., Takeda, N., Nakanishi, M. (2005). Depletion of Chk1 Leads to Premature Activation of Cdc2-cyclin B and Mitotic Catastrophe. J. Biol. Chem. 280: 39246-39252 [Abstract] [Full Text]  
  • Li, X., Malathi, K., Krizanova, O., Ondrias, K., Sperber, K., Ablamunits, V., Jayaraman, T. (2005). Cdc2/Cyclin B1 Interacts with and Modulates Inositol 1,4,5-Trisphosphate Receptor (Type 1) Functions. J. Immunol. 175: 6205-6210 [Abstract] [Full Text]  
  • Acheampong, E. A., Parveen, Z., Muthoga, L. W., Wasmuth-Peroud, V., Kalayeh, M., Bashir, A., Diecidue, R., Mukhtar, M., Pomerantz, R. J. (2005). Molecular Interactions of Human Immunodeficiency Virus Type 1 with Primary Human Oral Keratinocytes. J. Virol. 79: 8440-8453 [Abstract] [Full Text]  
  • Torgler, R., Jakob, S., Ontsouka, E., Nachbur, U., Mueller, C., Green, D. R., Brunner, T. (2004). Regulation of Activation-induced Fas (CD95/Apo-1) Ligand Expression in T Cells by the Cyclin B1/Cdk1 Complex. J. Biol. Chem. 279: 37334-37342 [Abstract] [Full Text]  
  • Jackson, S. J. T., Singletary, K. W. (2004). Sulforaphane Inhibits Human MCF-7 Mammary Cancer Cell Mitotic Progression and Tubulin Polymerization. J. Nutr. 134: 2229-2236 [Abstract] [Full Text]  
  • Lawson, B. R., Baccala, R., Song, J., Croft, M., Kono, D. H., Theofilopoulos, A. N. (2004). Deficiency of the Cyclin Kinase Inhibitor p21(WAF-1/CIP-1) Promotes Apoptosis of Activated/Memory T Cells and Inhibits Spontaneous Systemic Autoimmunity. JEM 199: 547-557 [Abstract] [Full Text]  
  • Jackson, S. J. T., Singletary, K. W. (2004). Sulforaphane: a naturally occurring mammary carcinoma mitotic inhibitor, which disrupts tubulin polymerization. Carcinogenesis 25: 219-227 [Abstract] [Full Text]  
  • Quemeneur, L., Gerland, L.-M., Flacher, M., Ffrench, M., Revillard, J.-P., Genestier, L. (2003). Differential Control of Cell Cycle, Proliferation, and Survival of Primary T Lymphocytes by Purine and Pyrimidine Nucleotides. J. Immunol. 170: 4986-4995 [Abstract] [Full Text]  
  • Porter, L. A., Cukier, I. H., Lee, J. M. (2003). Nuclear localization of cyclin B1 regulates DNA damage-induced apoptosis. Blood 101: 1928-1933 [Abstract] [Full Text]  
  • Adachi, S., Obaya, A. J., Han, Z., Ramos-Desimone, N., Wyche, J. H., Sedivy, J. M. (2001). c-Myc Is Necessary for DNA Damage-Induced Apoptosis in the G2 Phase of the Cell Cycle. Mol. Cell. Biol. 21: 4929-4937 [Abstract] [Full Text]  
  • Cannavo', G., Paiardini, M., Galati, D., Cervasi, B., Montroni, M., De Vico, G., Guetard, D., Bocchino, M. L., Picerno, I., Magnani, M., Silvestri, G., Piedimonte, G. (2001). Abnormal intracellular kinetics of cell-cycle-dependent proteins in lymphocytes from patients infected with human immunodeficiency virus: a novel biologic link between immune activation, accelerated T-cell turnover, and high levels of apoptosis. Blood 97: 1756-1764 [Abstract] [Full Text]  
  • Adachi, S., Ito, H., Tamamori-Adachi, M., Ono, Y., Nozato, T., Abe, S., Ikeda, M.-a., Marumo, F., Hiroe, M. (2001). Cyclin A/cdk2 Activation Is Involved in Hypoxia-Induced Apoptosis in Cardiomyocytes. Circ. Res. 88: 408-414 [Abstract] [Full Text]  
  • Carpenter, P. A., Pavlovic, S., Tso, J. Y., Press, O. W., Gooley, T., Yu, X.-Z., Anasetti, C. (2000). Non-Fc Receptor-Binding Humanized Anti-CD3 Antibodies Induce Apoptosis of Activated Human T Cells. J. Immunol. 165: 6205-6213 [Abstract] [Full Text]  
  • Porter, L. A., Singh, G., Lee, J. M. (2000). Abundance of cyclin B1 regulates gamma -radiation-induced apoptosis. Blood 95: 2645-2650 [Abstract] [Full Text]  
  • Harvey, K. J., Lukovic, D., Ucker, D. S. (2000). Caspase-Dependent Cdk Activity Is a Requisite Effector of Apoptotic Death Events. JCB 148: 59-72 [Abstract] [Full Text]  
  • Frade, J. (2000). Unscheduled re-entry into the cell cycle induced by NGF precedes cell death in nascent retinal neurones. J. Cell Sci. 113: 1139-1148 [Abstract]  
  • Karas, M., Zaks, T. Z., JL, L., LeRoith, D. (1999). T Cell Receptor-induced Activation and Apoptosis In Cycling Human T Cells Occur throughout the Cell Cycle. Mol. Biol. Cell 10: 4441-4450 [Abstract] [Full Text]  
  • Choi, K. S., Eom, Y. W., Kang, Y., Ha, M. J., Rhee, H., Yoon, J.-W., Kim, S.-J. (1999). Cdc2 and Cdk2 Kinase Activated by Transforming Growth Factor-beta 1 Trigger Apoptosis through the Phosphorylation of Retinoblastoma Protein in FaO Hepatoma Cells. J. Biol. Chem. 274: 31775-31783 [Abstract] [Full Text]  
  • Wu, L., Chen, P., Hwang, J.-J., Barsky, L. W., Weinberg, K. I., Jong, A., Starnes, V. A. (1999). RNA Antisense Abrogation of MAT1 Induces G1 Phase Arrest and Triggers Apoptosis in Aortic Smooth Muscle Cells. J. Biol. Chem. 274: 5564-5572 [Abstract] [Full Text]  
  • Secchiero, P., Bertolaso, L., Casareto, L., Gibellini, D., Vitale, M., Bemis, K., Aleotti, A., Capitani, S., Franchini, G., Gallo, R. C., Zauli, G. (1998). Human Herpesvirus 7 Infection Induces Profound Cell Cycle Perturbations Coupled to Disregulation of cdc2 and Cyclin B and Polyploidization of CD4+ T Cells. Blood 92: 1685-1696 [Abstract] [Full Text]  
  • Chen, C.-Y., Liou, J., Forman, L. W., Faller, D. V. (1998). Differential Regulation of Discrete Apoptotic Pathways by Ras. J. Biol. Chem. 273: 16700-16709 [Abstract] [Full Text]  
  • Harvey, K. J., Blomquist, J. F., Ucker, D. S. (1998). Commitment and Effector Phases of the Physiological Cell Death Pathway Elucidated with Respect to Bcl-2, Caspase, and Cyclin-Dependent Kinase Activities. Mol. Cell. Biol. 18: 2912-2922 [Abstract] [Full Text]  
  • Duband-Goulet, I, Courvalin, J., Buendia, B (1998). LBR, a chromatin and lamin binding protein from the inner nuclear membrane, is proteolyzed at late stages of apoptosis. J. Cell Sci. 111: 1441-1451 [Abstract]  
  • Brenner, B., Koppenhoefer, U., Weinstock, C., Linderkamp, O., Lang, F., Gulbins, E. (1997). Fas- or Ceramide-induced Apoptosis Is Mediated by a Rac1-regulated Activation of Jun N-terminal Kinase/p38 Kinases and GADD153. J. Biol. Chem. 272: 22173-22181 [Abstract] [Full Text]  
  • Desrivieres, S., Desrivières, S., Ferrari, S. (1997). Evidence for Different Mechanisms of Growth Inhibition of T-cell Lymphoma by Phorbol Esters and Concanavalin A. J. Biol. Chem. 272: 2470-2476 [Abstract] [Full Text]  
  • Wright, D. A., Futcher, B., Ghosh, P., Geha, R. S. (1996). Association of Human Fas (CD95) with a Ubiquitin-conjugating Enzyme (UBC-FAP). J. Biol. Chem. 271: 31037-31043 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»