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Mol. Cell. Biol., 05 1995, 2448-2456, Vol 15, No. 5
Copyright © 1995, American Society for Microbiology

Self-association of the erythroid transcription factor GATA-1 mediated by its zinc finger domains

M Crossley, M Merika and SH Orkin
Division of Hematology-Oncology, Children's Hospital, Boston, Massachusetts 02115, USA.

GATA-1, the founding member of a distinctive family of transcription factors, is expressed predominantly in erythroid cells and participates in the expression of numerous erythroid cell-expressed genes. GATA- binding sites are found in the promoters and enhancers of globin and nonglobin erythroid genes as well as in the alpha- and beta-globin locus control regions. To elucidate how GATA-1 may function in a variety of regulatory contexts, we have examined its protein-protein interactions. Here we show that GATA-1 self-associates in solution and in whole-cell extracts and that the zinc finger region of the molecule is sufficient to mediate this interaction. This physical interaction can influence transcription, as GATA-1 self-association is able to recruit a transcriptionally active but DNA-binding-defective derivative of GATA-1 to promoter-bound GATA-1 and result in superactivation. Through in vitro studies with bacterially expressed glutathione S- transferase fusion proteins, we have localized the minimal domain required for GATA-1 self-association to 40 amino acid residues within the C-terminal zinc finger region. Finally, we have detected physical interaction of GATA-1 with other GATA family members (GATA-2 and GATA- 3) also mediated through the zinc finger domain. These findings have broad implications for the involvement of GATA factors in transcriptional control. In particular, the interaction of GATA-1 with itself and with other transcription factors may facilitate its function at diverse promoters in erythroid cells and also serve to bring together, or stabilize, loops between distant regulatory elements, such as the globin locus control regions and downstream globin promoters.(ABSTRACT TRUNCATED AT 250 WORDS)


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  • Sepulveda, J. L., Belaguli, N., Nigam, V., Chen, C.-Y., Nemer, M., Schwartz, R. J. (1998). GATA-4 and Nkx-2.5 Coactivate Nkx-2 DNA Binding Targets: Role for Regulating Early Cardiac Gene Expression. Mol. Cell. Biol. 18: 3405-3415 [Abstract] [Full Text]  
  • Igarashi, K., Hoshino, H., Muto, A., Suwabe, N., Nishikawa, S., Nakauchi, H., Yamamoto, M. (1998). Multivalent DNA Binding Complex Generated by Small Maf and Bach1 as a Possible Biochemical Basis for beta -Globin Locus Control Region Complex. J. Biol. Chem. 273: 11783-11790 [Abstract] [Full Text]  
  • Blobel, G. A., Nakajima, T., Eckner, R., Montminy, M., Orkin, S. H. (1998). CREB-binding protein cooperates with transcription factor GATA-1 and is required for erythroid differentiation. Proc. Natl. Acad. Sci. USA 95: 2061-2066 [Abstract] [Full Text]  
  • Feng, Z.-M., Wu, A. Z., Chen, C.-L. C. (1998). Testicular GATA-1 Factor Up-Regulates the Promoter Activity of Rat Inhibin {alpha}-Subunit Gene in MA-10 Leydig Tumor Cells. Mol. Endocrinol. 12: 378-390 [Abstract] [Full Text]  
  • Haenlin, M., Cubadda, Y., Blondeau, F., Heitzler, P., Lutz, Y., Simpson, P., Ramain, P. (1997). Transcriptional activity of Pannier is regulated negatively by heterodimerization of the GATA DNA-binding domain with a cofactor encoded by the u-shaped gene of Drosophila. Genes Dev. 11: 3096-3108 [Abstract] [Full Text]  
  • Zhang, X., Xing, G., Fraizer, G. C., Saunders, G. F. (1997). Transactivation of an Intronic Hematopoietic-specific Enhancer of the Human Wilms' Tumor 1 Gene by GATA-1 and c-Myb. J. Biol. Chem. 272: 29272-29280 [Abstract] [Full Text]  
  • Surinya, K. H., Cox, T. C., May, B. K. (1997). Transcriptional Regulation of the Human Erythroid 5-Aminolevulinate Synthase Gene. IDENTIFICATION OF PROMOTER ELEMENTS AND ROLE OF REGULATORY PROTEINS. J. Biol. Chem. 272: 26585-26594 [Abstract] [Full Text]  
  • Haas, H., Angermayr, K., Zadra, I., Stoffler, G. (1997). Overexpression of nreB, a New GATA Factor-encoding Gene of Penicillium chrysogenum, Leads to Repression of the Nitrate Assimilatory Gene Cluster. J. Biol. Chem. 272: 22576-22582 [Abstract] [Full Text]  
  • Heikinheimo, M., Ermolaeva, M., Bielinska, M., Rahman, N. A., Narita, N., Huhtaniemi, I. T., Tapanainen, J. S., Wilson, D. B. (1997). Expression and Hormonal Regulation of Transcription Factors GATA-4 and GATA-6 in the Mouse Ovary. Endocrinology 138: 3505-3514 [Abstract] [Full Text]  
  • Kuo, C T, Morrisey, E E, Anandappa, R, Sigrist, K, Lu, M M, Parmacek, M S, Soudais, C, Leiden, J M (1997). GATA4 transcription factor is required for ventral morphogenesis and heart tube formation.. Genes Dev. 11: 1048-1060 [Abstract]  
  • Artsimovitch, I., Murakami, K., Ishihama, A., Howe, M. M. (1996). Transcription Activation by the Bacteriophage Mu Mor Protein Requires the C-terminal Regions of Both alpha and sigma 70 Subunits of Escherichia coli RNA Polymerase. J. Biol. Chem. 271: 32343-32348 [Abstract] [Full Text]  
  • Mably, J. D., Liew, C.-C. (1996). Factors Involved in Cardiogenesis and the Regulation of Cardiac-Specific Gene Expression. Circ. Res. 79: 4-13 [Abstract] [Full Text]  
  • Bushmeyer, S., Park, K., Atchison, M. L. (1995). Characterization of Functional Domains within the Multifunctional Transcription Factor, YY1. J. Biol. Chem. 270: 30213-30220 [Abstract] [Full Text]  
  • Newton, A., Mackay, J., Crossley, M. (2001). The N-terminal Zinc Finger of the Erythroid Transcription Factor GATA-1 Binds GATC Motifs in DNA. J. Biol. Chem. 276: 35794-35801 [Abstract] [Full Text]  
  • Hikke van Doorninck, J., van der Wees, J., Karis, A., Goedknegt, E., Coesmans, M., Rutteman, M., Grosveld, F., De Zeeuw, C. I. (1999). GATA-3 Is Involved in the Development of Serotonergic Neurons in the Caudal Raphe Nuclei. J. Neurosci. 19: RC12-RC12 [Abstract] [Full Text]  


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