This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Orren, D. K.
Right arrow Articles by Bohr, V. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Orren, D. K.
Right arrow Articles by Bohr, V. A.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Jul 1995, 3722-3730, Vol 15, No. 7
Copyright © 1995, American Society for Microbiology

A UV-responsive G2 checkpoint in rodent cells

DK Orren, LN Petersen and VA Bohr
Laboratory of Molecular Genetics, National Institute on Aging, Baltimore, Maryland 21224, USA.

We have studied the effect of UV irradiation on the cell cycle progression of synchronized Chinese hamster ovary cells. Synchronization of cells in S or G2 phase was accomplished by the development of a novel protocol using mimosine, which blocks cell cycle progression at the G1/S boundary. After removal of mimosine, cells proceed synchronously through the S and G2 phases, allowing manipulation of cells at specific points in either phase. Synchronization of cells in G1 was achieved by release of cells after a period of serum starvation. Cells synchronized by these methods were UV irradiated at defined points in G1, S, and G2, and their subsequent progression through the cell cycle was monitored. UV irradiation of G1- synchronized cells caused a dose-dependent delay in entry into S phase. Irradiation of S-phase-synchronized cells inhibited progression through S phase and then resulted in accumulation of cells for a prolonged interval in G2. Apoptosis of a subpopulation of cells during this extended period was noted. UV irradiation of G2-synchronized cells caused a shorter G2 arrest. The arrest itself and its duration were dependent upon the timing (within G2 phase) of the irradiation and the UV dose, respectively. We have thus defined a previously undescribed (in mammalian cells) UV-responsive checkpoint in G2 phase. The implications of these findings with respect to DNA metabolism are discussed.


This article has been cited by other articles:

  • Knezevic, D., Zhang, W., Rochette, P. J., Brash, D. E. (2007). Bcl-2 is the target of a UV-inducible apoptosis switch and a node for UV signaling. Proc. Natl. Acad. Sci. USA 104: 11286-11291 [Abstract] [Full Text]  
  • Andreassen, P. R., Ho, G. P.H., D'Andrea, A. D. (2006). DNA damage responses and their many interactions with the replication fork. Carcinogenesis 27: 883-892 [Abstract] [Full Text]  
  • Richards, S., Liu, S.-T., Majumdar, A., Liu, J.-L., Nairn, R. S., Bernier, M., Maher, V., Seidman, M. M. (2005). Triplex targeted genomic crosslinks enter separable deletion and base substitution pathways. Nucleic Acids Res 33: 5382-5393 [Abstract] [Full Text]  
  • Shimada, M., Nakadai, T., Tamura, T.-a. (2003). TATA-Binding Protein-Like Protein (TLP/TRF2/TLF) Negatively Regulates Cell Cycle Progression and Is Required for the Stress-Mediated G2 Checkpoint. Mol. Cell. Biol. 23: 4107-4120 [Abstract] [Full Text]  
  • Majumdar, A., Puri, N., Cuenoud, B., Natt, F., Martin, P., Khorlin, A., Dyatkina, N., George, A. J., Miller, P. S., Seidman, M. M. (2003). Cell Cycle Modulation of Gene Targeting by a Triple Helix-forming Oligonucleotide. J. Biol. Chem. 278: 11072-11077 [Abstract] [Full Text]  
  • Zhang, H., Taylor, J., Siede, W. (2003). Checkpoint Arrest Signaling in Response to UV Damage Is Independent of Nucleotide Excision Repair in Saccharomyces cerevisiae. J. Biol. Chem. 278: 9382-9387 [Abstract] [Full Text]  
  • Weiss, R. S., Leder, P., Vaziri, C. (2003). Critical Role for Mouse Hus1 in an S-Phase DNA Damage Cell Cycle Checkpoint. Mol. Cell. Biol. 23: 791-803 [Abstract] [Full Text]  
  • Murphy, M, Mabruk, M J E M F, Lenane, P, Liew, A, McCann, P, Buckley, A, Flatharta, C O, Hevey, D, Billet, P, Robertson, W, Javed, S, Leader, M, Kay, E, Murphy, G M (2002). Comparison of the expression of p53, p21, Bax and the induction of apoptosis between patients with basal cell carcinoma and normal controls in response to ultraviolet irradiation. J. Clin. Pathol. 55: 829-833 [Abstract] [Full Text]  
  • Taylor, R. M., Thistlethwaite, A., Caldecott, K. W. (2002). Central Role for the XRCC1 BRCT I Domain in Mammalian DNA Single-Strand Break Repair. Mol. Cell. Biol. 22: 2556-2563 [Abstract] [Full Text]  
  • Rodrigo, G., Roumagnac, S., Wold, M. S., Salles, B., Calsou, P. (2000). DNA Replication but Not Nucleotide Excision Repair Is Required for UVC-Induced Replication Protein A Phosphorylation in Mammalian Cells. Mol. Cell. Biol. 20: 2696-2705 [Abstract] [Full Text]  
  • Taylor, R. M., Moore, D. J., Whitehouse, J., Johnson, P., Caldecott, K. W. (2000). A Cell Cycle-Specific Requirement for the XRCC1 BRCT II Domain during Mammalian DNA Strand Break Repair. Mol. Cell. Biol. 20: 735-740 [Abstract] [Full Text]  
  • RIEDER, C.L., COLE, R. (2000). Microscopy-induced Radiation Damage, Microtubules, and Progression through the Terminal Stage of G2 (Prophase) in Vertebrate Somatic Cells. Cold Spring Harb Symp Quant Biol 65: 369-376 [Abstract]  
  • Bordignon, V., Smith, L. C. (1999). Ultraviolet-Irradiated Spermatozoa Activate Oocytes but Arrest Preimplantation Development After Fertilization and Nuclear Transplantation in Cattle. Biol. Reprod. 61: 1513-1520 [Abstract] [Full Text]  
  • Johnson, P. A., Clements, P., Hudson, K., Caldecott, K. W. (1999). A Mitotic Spindle Requirement for DNA Damage-induced Apoptosis in Chinese Hamster Ovary Cells. Cancer Res. 59: 2696-2700 [Abstract] [Full Text]  
  • Chang, D., Chen, F., Zhang, F., McKay, B. C., Ljungman, M. (1999). Dose-dependent Effects of DNA-damaging Agents on p53-mediated Cell Cycle Arrest. Cell Growth Differ. 10: 155-162 [Abstract] [Full Text]  
  • Manabe, R.-i., Oh-e, N., Sekiguchi, K. (1999). Alternatively Spliced EDA Segment Regulates Fibronectin-dependent Cell Cycle Progression and Mitogenic Signal Transduction. J. Biol. Chem. 274: 5919-5924 [Abstract] [Full Text]  
  • Melton, D., Ketchen, A., Nunez, F, Bonatti-Abbondandolo, S, Abbondandolo, A, Squires, S, Johnson, R. (1998). Cells from ERCC1-deficient mice show increased genome instability and a reduced frequency of S-phase-dependent illegitimate chromosome exchange but a normal frequency of homologous recombination. J. Cell Sci. 111: 395-404 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»