This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sueyoshi, T.
Right arrow Articles by Negishi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sueyoshi, T.
Right arrow Articles by Negishi, M.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 08 1995, 4158-4166, Vol 15, No. 8

A nuclear factor (NF2d9) that binds to the male-specific P450 (Cyp 2d- 9) gene in mouse liver

T Sueyoshi, R Kobayashi, K Nishio, K Aida, R Moore, T Wada, H Handa and M Negishi
Pharmacogenetics Section, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA.

Expression of the Cyp 2d-9 (steroid 16 alpha-hydroxylase) gene in mouse liver is male specific in such Mus musculus domesticus strains as FVB/N, whereas the corresponding P450 genes in the wild mouse species Mus spretus are not sex specific in their expression. These parental differences in the gene expressions were independently inherited in F1 offspring from crosses of FVB/N and M. spretus. A 5' flanking sequence (-110CTC CTCCCTATTCCGGGCC-92) was defined as a regulatory element (named SDI-A1) for the domestic Cyp 2d-9 promoter. The nucleotide which corresponds to T at position -99 within SDI-A1 was found to be substituted with C in the wild mouse P450 genes. The placing of C at position -99 abolished the transcriptional activity of SDI-A1 in HepG2 cells as well as the binding of SDI-A1 to a nuclear factor. This factor (designated NF2d9) was purified from mouse nuclear extracts, and its cDNA cloned. The purified NF2d9 bound to SDI-A1 but not to the mutated SDI-A1 with C at position -99. The deduced amino acid sequence revealed that NF2d9 is 72 and 94% identical to mouse CP2 and human LBP-1a, respectively. NF2d9 thus belongs to the CP2 family and is the mouse homolog of human LBP-1a, which modulates human immunodeficiency virus type 1 transcription. Anti-NF2d9, which was raised against the bacterially expressed protein, supershifted the SDI-A1 complex with the liver nuclear extract. Both the bacterially expressed and in vitro- translated NF2d9 inhibited SDI-A1 complex formation, although they did not bind to SDI-A1 directly. The results, therefore, indicate that the domestic Cyp 2d-9 gene can be regulated through a specific association of NF2d9 with SDI-A1.


This article has been cited by other articles:

  • Katsura, A., Kimura, K., Hosoi, K., Tomokuni, Y., Nesori, M., Goryo, K., Numayama-Tsuruta, K., Torii, S., Yasumoto, K.-i., Gotoh, O., Takada, M., Fukumura, H., Sogawa, K. (2009). Transactivation activity of LBP-1 proteins and their dimerization in living cells. GENES CELLS 14: 1183-1196 [Abstract] [Full Text]  
  • Merrell, M. D., Jackson, J. P., Augustine, L. M., Fisher, C. D., Slitt, A. L., Maher, J. M., Huang, W., Moore, D. D., Zhang, Y., Klaassen, C. D., Cherrington, N. J. (2008). The Nrf2 Activator Oltipraz Also Activates the Constitutive Androstane Receptor. Drug Metab. Dispos. 36: 1716-1721 [Abstract] [Full Text]  
  • Yamaguchi, Y., Yonemura, S., Takada, S. (2006). Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse. Development 133: 4737-4748 [Abstract] [Full Text]  
  • Waxman, D. J., O'Connor, C. (2006). Growth Hormone Regulation of Sex-Dependent Liver Gene Expression. Mol. Endocrinol. 20: 2613-2629 [Abstract] [Full Text]  
  • Sato, F., Yasumoto, K.-i., Kimura, K., Numayama-Tsuruta, K., Sogawa, K. (2005). Heterodimerization with LBP-1b is necessary for nuclear localization of LBP-1a and LBP-1c. GENES CELLS 10: 861-870 [Abstract] [Full Text]  
  • Squires, E. J., Sueyoshi, T., Negishi, M. (2004). Cytoplasmic Localization of Pregnane X Receptor and Ligand-dependent Nuclear Translocation in Mouse Liver. J. Biol. Chem. 279: 49307-49314 [Abstract] [Full Text]  
  • Parekh, V., McEwen, A., Barbour, V., Takahashi, Y., Rehg, J. E., Jane, S. M., Cunningham, J. M. (2004). Defective Extraembryonic Angiogenesis in Mice Lacking LBP-1a, a Member of the grainyhead Family of Transcription Factors. Mol. Cell. Biol. 24: 7113-7129 [Abstract] [Full Text]  
  • Jackson, J. P., Ferguson, S. S., Moore, R., Negishi, M., Goldstein, J. A. (2004). The Constitutive Active/Androstane Receptor Regulates Phenytoin Induction of Cyp2c29. Mol. Pharmacol. 65: 1397-1404 [Abstract] [Full Text]  
  • Waxman, D. J., Celenza, J. L. (2003). Sexual dimorphism of hepatic gene expression: novel biological role of KRAB zinc finger repressors revealed. Genes Dev. 17: 2607-2613 [Full Text]  
  • Venkatesan, K., McManus, H. R., Mello, C. C., Smith, T. F., Hansen, U. (2003). Functional conservation between members of an ancient duplicated transcription factor family, LSF/Grainyhead. Nucleic Acids Res 31: 4304-4316 [Abstract] [Full Text]  
  • Peters, L. M., Anderson, D. W., Griffith, A. J., Grundfast, K. M., San Agustin, T. B., Madeo, A. C., Friedman, T. B., Morell, R. J. (2002). Mutation of a transcription factor, TFCP2L3, causes progressive autosomal dominant hearing loss, DFNA28. Hum Mol Genet 11: 2877-2885 [Abstract] [Full Text]  
  • Xiong, H., Yoshinari, K., Brouwer, K. L. R., Negishi, M. (2002). Role of Constitutive Androstane Receptor in the In Vivo Induction of Mrp3 and CYP2B1/2 by Phenobarbital. Drug Metab. Dispos. 30: 918-923 [Abstract] [Full Text]  
  • Tuckfield, A., Clouston, D. R., Wilanowski, T. M., Zhao, L.-L., Cunningham, J. M., Jane, S. M. (2002). Binding of the RING Polycomb Proteins to Specific Target Genes in Complex with the grainyhead-Like Family of Developmental Transcription Factors. Mol. Cell. Biol. 22: 1936-1946 [Abstract] [Full Text]  
  • Van Ess, P. J., Mattson, M. P., Blouin, R. A. (2002). Enhanced Induction of Cytochrome P450 Enzymes and CAR Binding in TNF (p55-/-/p75-/-) Double Receptor Knockout Mice Following Phenobarbital Treatment. J. Pharmacol. Exp. Ther. 300: 824-830 [Abstract] [Full Text]  
  • Sueyoshi, T., Yokomori, N., Korach, K. S., Negishi, M. (1999). Developmental Action of Estrogen Receptor-alpha Feminizes the Growth Hormone-Stat5b Pathway and Expression of Cyp2a4 and Cyp2d9 Genes in Mouse Liver. Mol. Pharmacol. 56: 473-477 [Abstract] [Full Text]  
  • Honkakoski, P., Zelko, I., Sueyoshi, T., Negishi, M. (1998). The Nuclear Orphan Receptor CAR-Retinoid X Receptor Heterodimer Activates the Phenobarbital-Responsive Enhancer Module of the CYP2B Gene. Mol. Cell. Biol. 18: 5652-5658 [Abstract] [Full Text]  
  • Shirra, M. K., Hansen, U. (1998). LSF and NTF-1 Share a Conserved DNA Recognition Motif yet Require Different Oligomerization States to Form a Stable Protein-DNA Complex. J. Biol. Chem. 273: 19260-19268 [Abstract] [Full Text]  
  • Masubuchi, Y., Iwasa, T., Hosokawa, S., Suzuki, T., Horie, T., Imaoka, S., Funae, Y., Narimatsu, S. (1997). Selective Deficiency of Debrisoquine 4-Hydroxylase Activity in Mouse Liver Microsomes. J. Pharmacol. Exp. Ther. 282: 1435-1441 [Abstract] [Full Text]  
  • Watakabe, A, Kobayashi, R, Helfman, D. (1996). N-tropomodulin: a novel isoform of tropomodulin identified as the major binding protein to brain tropomyosin. J. Cell Sci. 109: 2299-2310 [Abstract]  
  • Ramamurthy, L., Barbour, V., Tuckfield, A., Clouston, D. R., Topham, D., Cunningham, J. M., Jane, S. M. (2001). Targeted Disruption of the CP2 Gene, a Member of the NTF Family of Transcription Factors. J. Biol. Chem. 276: 7836-7842 [Abstract] [Full Text]  
  • Casolaro, V., Keane-Myers, A. M., Swendeman, S. L., Steindler, C., Zhong, F., Sheffery, M., Georas, S. N., Ono, S. J. (2000). Identification and Characterization of a Critical CP2-binding Element in the Human Interleukin-4 Promoter. J. Biol. Chem. 275: 36605-36611 [Abstract] [Full Text]  
  • Rodda, S., Sharma, S., Scherer, M., Chapman, G., Rathjen, P. (2001). CRTR-1, a Developmentally Regulated Transcriptional Repressor Related to the CP2 Family of Transcription Factors. J. Biol. Chem. 276: 3324-3332 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»