This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Braun, B. S.
Right arrow Articles by Denny, C. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Braun, B. S.
Right arrow Articles by Denny, C. T.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 08 1995, 4623-4630, Vol 15, No. 8
Copyright © 1995, American Society for Microbiology

Identification of target genes for the Ewing's sarcoma EWS/FLI fusion protein by representational difference analysis

BS Braun, R Frieden, SL Lessnick, WA May and CT Denny
Molecular Biology Institute, University of California, Los Angeles 90024, USA.

The EWS/FLI-1 fusion gene results from the 11;22 chromosomal translocation in Ewing's sarcoma. The product of the gene is one of a growing number of structurally altered transcription factors implicated in oncogenesis. We have employed a subtractive cloning strategy of representational difference analysis in conjunction with a model transformation system to identify genes transcribed in response to EWS/FLI. We have characterized eight transcripts that are dependent on EWS/FLI for expression and two transcripts that are repressed in response to EWS/FLI. Three of the former were identified by sequence analysis as stromelysin 1, a murine homolog of cytochrome P-450 F1 and cytokeratin 15. Stromelysin 1 is induced rapidly after expression of EWS/FLI, suggesting that the stromelysin 1 gene may be a direct target gene of EWS/FLI. These results demonstrate that expression of EWS/FLI leads to significant changes in the transcription of specific genes and that these effects are at least partially distinct from those caused by expression of germ line FLI-1. The representational difference analysis technique can potentially be applied to investigate transformation pathways activated by a broad array of genes in different tumor systems.


This article has been cited by other articles:

  • Lin, P. P., Pandey, M. K., Jin, F., Xiong, S., Deavers, M., Parant, J. M., Lozano, G. (2008). EWS-FLI1 Induces Developmental Abnormalities and Accelerates Sarcoma Formation in a Transgenic Mouse Model. Cancer Res. 68: 8968-8975 [Abstract] [Full Text]  
  • Riggi, N., Cironi, L., Provero, P., Suva, M.-L., Stehle, J.-C., Baumer, K., Guillou, L., Stamenkovic, I. (2006). Expression of the FUS-CHOP Fusion Protein in Primary Mesenchymal Progenitor Cells Gives Rise to a Model of Myxoid Liposarcoma.. Cancer Res. 66: 7016-7023 [Abstract] [Full Text]  
  • Basler, T., Jeckstadt, S., Valentin-Weigand, P., Goethe, R. (2006). Mycobacterium paratuberculosis, Mycobacterium smegmatis, and lipopolysaccharide induce different transcriptional and post-transcriptional regulation of the IRG1 gene in murine macrophages. J. Leukoc. Biol. 79: 628-638 [Abstract] [Full Text]  
  • Riggi, N., Cironi, L., Provero, P., Suva, M.-L., Kaloulis, K., Garcia-Echeverria, C., Hoffmann, F., Trumpp, A., Stamenkovic, I. (2005). Development of Ewing's Sarcoma from Primary Bone Marrow-Derived Mesenchymal Progenitor Cells. Cancer Res. 65: 11459-11468 [Abstract] [Full Text]  
  • Ano, S., Pereira, R., Pironin, M., Lesault, I., Milley, C., Lebigot, I., Quang, C. T., Ghysdael, J. (2004). Erythroblast Transformation by FLI-1 Depends upon Its Specific DNA Binding and Transcriptional Activation Properties. J. Biol. Chem. 279: 2993-3002 [Abstract] [Full Text]  
  • Deneen, B., Hamidi, H., Denny, C. T. (2003). Functional Analysis of the EWS/ETS Target Gene Uridine Phosphorylase. Cancer Res. 63: 4268-4274 [Abstract] [Full Text]  
  • Deneen, B., Welford, S. M., Ho, T., Hernandez, F., Kurland, I., Denny, C. T. (2003). PIM3 Proto-Oncogene Kinase Is a Common Transcriptional Target of Divergent EWS/ETS Oncoproteins. Mol. Cell. Biol. 23: 3897-3908 [Abstract] [Full Text]  
  • Burchill, S A (2003). Ewing's sarcoma: diagnostic, prognostic, and therapeutic implications of molecular abnormalities. J. Clin. Pathol. 56: 96-102 [Abstract] [Full Text]  
  • Sasagawa, K., Matsudo, Y., Kang, M., Fujimura, L., Iitsuka, Y., Okada, S., Ochiai, T., Tokuhisa, T., Hatano, M. (2002). Identification of Nd1, a Novel Murine Kelch Family Protein, Involved in Stabilization of Actin Filaments. J. Biol. Chem. 277: 44140-44146 [Abstract] [Full Text]  
  • Martini, A., La Starza, R., Janssen, H., Bilhou-Nabera, C., Corveleyn, A., Somers, R., Aventin, A., Foa, R., Hagemeijer, A., Mecucci, C., Marynen, P. (2002). Recurrent Rearrangement of the Ewing's Sarcoma Gene, EWSR1, or Its Homologue, TAF15, with the Transcription Factor CIZ/NMP4 in Acute Leukemia. Cancer Res. 62: 5408-5412 [Abstract] [Full Text]  
  • Sturm, R. A., Satyamoorthy, K., Meier, F., Gardiner, B. B., Smit, D. J., Vaidya, B., Herlyn, M. (2002). Osteonectin/SPARC Induction by Ectopic {beta}3 Integrin in Human Radial Growth Phase Primary Melanoma Cells. Cancer Res. 62: 226-232 [Abstract] [Full Text]  
  • Welford, S. M., Hebert, S. P., Deneen, B., Arvand, A., Denny, C. T. (2001). DNA Binding Domain-independent Pathways Are Involved in EWS/FLI1-mediated Oncogenesis. J. Biol. Chem. 276: 41977-41984 [Abstract] [Full Text]  
  • Arvand, A., Welford, S. M., Teitell, M. A., Denny, C. T. (2001). The COOH-Terminal Domain of FLI-1 Is Necessary for Full Tumorigenesis and Transcriptional Modulation by EWS/FLI-1. Cancer Res. 61: 5311-5317 [Abstract] [Full Text]  
  • Marvin, M. J., Di Rocco, G., Gardiner, A., Bush, S. M., Lassar, A. B. (2001). Inhibition of Wnt activity induces heart formation from posterior mesoderm. Genes Dev. 15: 316-327 [Abstract] [Full Text]  
  • Wessely, O., De Robertis, E. M. (2000). The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation. Development 127: 2053-2062 [Abstract]  
  • Schmidt, M., Nazarov, V., Stevens, L., Watson, R., Wolff, L. (2000). Regulation of the Resident Chromosomal Copy of c-myc by c-Myb Is Involved in Myeloid Leukemogenesis. Mol. Cell. Biol. 20: 1970-1981 [Abstract] [Full Text]  
  • Liau, L. M., Lallone, R. L., Seitz, R. S., Buznikov, A., Gregg, J. P., Kornblum, H. I., Nelson, S. F., Bronstein, J. M. (2000). Identification of a Human Glioma-associated Growth Factor Gene, granulin, Using Differential Immuno-absorption. Cancer Res. 60: 1353-1360 [Abstract] [Full Text]  
  • de Alava, E., Panizo, A., Antonescu, C. R., Huvos, A. G., Pardo-Mindan, F. J., Barr, F. G., Ladanyi, M. (2000). Association of EWS-FLI1 Type 1 Fusion with Lower Proliferative Rate in Ewing’s Sarcoma. Am. J. Pathol. 156: 849-855 [Abstract] [Full Text]  
  • de Alava, E., Gerald, W. L. (2000). Molecular Biology of the Ewing’s Sarcoma/Primitive Neuroectodermal Tumor Family. JCO 18: 204-204 [Abstract] [Full Text]  
  • Boyd, K. E., Farnham, P. J. (1999). Identification of Target Genes of Oncogenic Transcription Factors. Exp. Biol. Med. 222: 9-28 [Abstract] [Full Text]  
  • Klingenspor, M., Xu, P., Cohen, R. D., Welch, C., Reue, K. (1999). Altered Gene Expression Pattern in the Fatty Liver Dystrophy Mouse Reveals Impaired Insulin-mediated Cytoskeleton Dynamics. J. Biol. Chem. 274: 23078-23084 [Abstract] [Full Text]  
  • Ginsberg, J. P., de Alava, E., Ladanyi, M., Wexler, L. H., Kovar, H., Paulussen, M., Zoubek, A., Dockhorn-Dworniczak, B., Juergens, H., Wunder, J. S., Andrulis, I. L., Malik, R., Sorensen, P. H.B., Womer, R. B., Barr, F. G. (1999). EWS-FLI1 and EWS-ERG Gene Fusions Are Associated With Similar Clinical Phenotypes in Ewing's Sarcoma. JCO 17: 1809-1809 [Abstract] [Full Text]  
  • Holmes, M. L., Haley, J. D., Cerruti, L., Zhou, W.-l., Zogos, H., Smith, D. E., Cunningham, J. M., Jane, S. M. (1999). Identification of Id2 as a Globin Regulatory Protein by Representational Difference Analysis of K562 Cells Induced To Express gamma -Globin with a Fungal Compound. Mol. Cell. Biol. 19: 4182-4190 [Abstract] [Full Text]  
  • Gu, J., Guan, X.-Y., Ashlock, M. A. (1999). Isolation of Human Transcripts Expressed in Hamster Cells from YACs by cDNA Representational Difference Analysis. Genome Res 9: 182-188 [Abstract] [Full Text]  
  • Kurosawa, H., Goi, K., Inukai, T., Inaba, T., Chang, K.-S., Shinjyo, T., Rakestraw, K. M., Naeve, C. W., Look, A. T. (1999). Two Candidate Downstream Target Genes for E2A-HLF. Blood 93: 321-332 [Abstract] [Full Text]  
  • Weng, Z., Fluckiger, A.-C., Nisitani, S., Wahl, M. I., Le, L. Q., Hunter, C. A., Fernal, A. A., Le Beau, M. M., Witte, O. N. (1998). A DNA damage and stress inducible G protein-coupled receptor blocks cells in G2/M. Proc. Natl. Acad. Sci. USA 95: 12334-12339 [Abstract] [Full Text]  
  • Zhang, D., Paley, A. J., Childs, G. (1998). The Transcriptional Repressor ZFM1 Interacts with and Modulates the Ability of EWS to Activate Transcription. J. Biol. Chem. 273: 18086-18091 [Abstract] [Full Text]  
  • Feldman, J. D., Vician, L., Crispino, M., Tocco, G., Marcheselli, V. L., Bazan, N. G., Baudry, M., Herschman, H. R. (1998). KID-1, a Protein Kinase Induced by Depolarization in Brain. J. Biol. Chem. 273: 16535-16543 [Abstract] [Full Text]  
  • Reiter, R. E., Gu, Z., Watabe, T., Thomas, G., Szigeti, K., Davis, E., Wahl, M., Nisitani, S., Yamashiro, J., Le Beau, M. M., Loda, M., Witte, O. N. (1998). Prostate stem cell antigen: A cell surface marker overexpressed in prostate cancer. Proc. Natl. Acad. Sci. USA 95: 1735-1740 [Abstract] [Full Text]  
  • Karnieli, E., Werner, H., Rauscher, F. J. III, Benjamin, L. E., LeRoith, D. (1996). The IGF-I Receptor Gene Promoter Is a Molecular Target for the Ewing's Sarcoma-Wilms' Tumor 1Fusion Protein. J. Biol. Chem. 271: 19304-19309 [Abstract] [Full Text]  
  • Knoop, L. L., Baker, S. J. (2000). The Splicing Factor U1C Represses EWS/FLI-mediated Transactivation. J. Biol. Chem. 275: 24865-24871 [Abstract] [Full Text]  
  • Yang, L., Chansky, H. A., Hickstein, D. D. (2000). EWS{middle dot}Fli-1 Fusion Protein Interacts with Hyperphosphorylated RNA Polymerase II and Interferes with Serine-Arginine Protein-mediated RNA Splicing. J. Biol. Chem. 275: 37612-37618 [Abstract] [Full Text]  
  • KIM, J., PELLETIER, J. (1999). Molecular genetics of chromosome translocations involving EWS and related family members. Physiol. Genomics 1: 127-138 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»