This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schlaepfer, D. D.
Right arrow Articles by Hunter, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schlaepfer, D. D.
Right arrow Articles by Hunter, T.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Oct 1996, 5623-5633, Vol 16, No. 10
Copyright © 1996, American Society for Microbiology

Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src-family protein-tyrosine kinases [published erratum appears in Mol Cell Biol 1996 Dec;16(12):7182-4]

DD Schlaepfer and T Hunter
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA.

Focal adhesion kinase (FAK) is a nonreceptor protein-tyrosine kinase (PTK) that associates with integrin receptors and participates in extracellular matrix-mediated signal transduction events. We showed previously that the c-Src nonreceptor PTK and the Grb2 SH2/SH3 adaptor protein bound directly to FAK after fibronectin stimulation (D. D. Schlaepfer, S.K. Hanks, T. Hunter, and P. van der Geer, Nature [London] 372:786-791, 1994). Here, we present evidence that c-Src association with FAK is required for Grb2 binding to FAK. Using a tryptic phosphopeptide mapping approach, the in vivo phosphorylation of the Grb2 binding site on FAK (Tyr-925) was detected after fibronectin stimulation of NIH 3T3 cells and was constitutively phosphorylated in v- Src-transformed NIH 3T3 cells. In vitro, c-Src phosphorylated FAK Tyr- 925 in a glutathione S-transferase-FAK C-terminal domain fusion protein, whereas FAK did not. Using epitope-tagged FAK constructs, transiently expressed in human 293 cells, we determined the effect of site-directed mutations on c-Src and Grb2 binding to FAK. Mutation of FAK Tyr-925 disrupted Grb2 binding, whereas mutation of the c-Src binding site on FAK (Tyr-397) disrupted both c-Src and Grb2 binding to FAK in vivo. These results support a model whereby Src-family PTKs are recruited to FAK and focal adhesions following integrin-induced autophosphorylation and exposure of FAK Tyr-397. Src-family binding and phosphorylation of FAK at Tyr-925 creates a Grb2 SH2-domain binding site and provides a link to the activation of the Ras signal transduction pathway. In Src-transformed cells, this pathway may be constitutively activated as a result of FAK Tyr-925 phosphorylation in the absence of integrin stimulation.


This article has been cited by other articles:

  • Koshman, Y. E., Engman, S. J., Kim, T., Iyengar, R., Henderson, K. K., Samarel, A. M. (2009). Role of FRNK tyrosine phosphorylation in vascular smooth muscle spreading and migration. Cardiovasc Res 0: cvp322v2-cvp322 [Abstract] [Full Text]  
  • Gupta, S. K., Vlahakis, N. E. (2009). Integrin {alpha}9{beta}1 mediates enhanced cell migration through nitric oxide synthase activity regulated by Src tyrosine kinase. J. Cell Sci. 122: 2043-2054 [Abstract] [Full Text]  
  • Papusheva, E., de Queiroz, F. M., Dalous, J., Han, Y., Esposito, A., Jares-Erijmanxa, E. A., Jovin, T. M., Bunt, G. (2009). Dynamic conformational changes in the FERM domain of FAK are involved in focal-adhesion behavior during cell spreading and motility. J. Cell Sci. 122: 656-666 [Abstract] [Full Text]  
  • Zouq, N. K., Keeble, J. A., Lindsay, J., Valentijn, A. J., Zhang, L., Mills, D., Turner, C. E., Streuli, C. H., Gilmore, A. P. (2009). FAK engages multiple pathways to maintain survival of fibroblasts and epithelia - differential roles for paxillin and p130Cas. J. Cell Sci. 122: 357-367 [Abstract] [Full Text]  
  • Wei, W.-C., Lin, H.-H., Shen, M.-R., Tang, M.-J. (2008). Mechanosensing machinery for cells under low substratum rigidity. Am. J. Physiol. Cell Physiol. 295: C1579-C1589 [Abstract] [Full Text]  
  • Provenzano, P. P., Inman, D. R., Eliceiri, K. W., Beggs, H. E., Keely, P. J. (2008). Mammary Epithelial-Specific Disruption of Focal Adhesion Kinase Retards Tumor Formation and Metastasis in a Transgenic Mouse Model of Human Breast Cancer. Am. J. Pathol. 173: 1551-1565 [Abstract] [Full Text]  
  • Cai, X., Lietha, D., Ceccarelli, D. F., Karginov, A. V., Rajfur, Z., Jacobson, K., Hahn, K. M., Eck, M. J., Schaller, M. D. (2008). Spatial and Temporal Regulation of Focal Adhesion Kinase Activity in Living Cells. Mol. Cell. Biol. 28: 201-214 [Abstract] [Full Text]  
  • Dise, R. S., Frey, M. R., Whitehead, R. H., Polk, D. B. (2008). Epidermal growth factor stimulates Rac activation through Src and phosphatidylinositol 3-kinase to promote colonic epithelial cell migration. Am. J. Physiol. Gastrointest. Liver Physiol. 294: G276-G285 [Abstract] [Full Text]  
  • Hayashida, T., Wu, M.-H., Pierce, A., Poncelet, A.-C., Varga, J., Schnaper, H. W. (2007). MAP-kinase activity necessary for TGFbeta1-stimulated mesangial cell type I collagen expression requires adhesion-dependent phosphorylation of FAK tyrosine 397. J. Cell Sci. 120: 4230-4240 [Abstract] [Full Text]  
  • Sanchez, V., Mahr, J. A., Orazio, N. I., Spector, D. H. (2007). Nuclear Export of the Human Cytomegalovirus Tegument Protein pp65 Requires Cyclin-Dependent Kinase Activity and the Crm1 Exporter. J. Virol. 81: 11730-11736 [Abstract] [Full Text]  
  • Maa, M.-C., Lee, J.-C., Chen, Y.-J., Chen, Y.-J., Lee, Y.-C., Wang, S.-T., Huang, C.-C., Chow, N.-H., Leu, T.-H. (2007). EPS8 Facilitates Cellular Growth and Motility of Colon Cancer Cells by Increasing the Expression and Activity of Focal Adhesion Kinase. J. Biol. Chem. 282: 19399-19409 [Abstract] [Full Text]  
  • Quadri, S. K., Bhattacharya, J. (2007). Resealing of endothelial junctions by focal adhesion kinase. Am. J. Physiol. Lung Cell. Mol. Physiol. 292: L334-L342 [Abstract] [Full Text]  
  • Mon, N. N., Hasegawa, H., Thant, A. A., Huang, P., Tanimura, Y., Senga, T., Hamaguchi, M. (2006). A Role for Focal Adhesion Kinase Signaling in Tumor Necrosis Factor-{alpha}-Dependent Matrix Metalloproteinase-9 Production in a Cholangiocarcinoma Cell Line, CCKS1.. Cancer Res. 66: 6778-6784 [Abstract] [Full Text]  
  • Bouchard, M. J., Wang, L., Schneider, R. J. (2006). Activation of Focal Adhesion Kinase by Hepatitis B Virus HBx Protein: Multiple Functions in Viral Replication. J. Virol. 80: 4406-4414 [Abstract] [Full Text]  
  • Ceccarelli, D. F. J., Song, H. K., Poy, F., Schaller, M. D., Eck, M. J. (2006). Crystal Structure of the FERM Domain of Focal Adhesion Kinase. J. Biol. Chem. 281: 252-259 [Abstract] [Full Text]  
  • Grigera, P. R., Jeffery, E. D., Martin, K. H., Shabanowitz, J., Hunt, D. F., Parsons, J. T. (2005). FAK phosphorylation sites mapped by mass spectrometry. J. Cell Sci. 118: 4931-4935 [Full Text]  
  • Hamadi, A., Bouali, M., Dontenwill, M., Stoeckel, H., Takeda, K., Ronde, P. (2005). Regulation of focal adhesion dynamics and disassembly by phosphorylation of FAK at tyrosine 397. J. Cell Sci. 118: 4415-4425 [Abstract] [Full Text]  
  • Urbinati, C., Bugatti, A., Giacca, M., Schlaepfer, D., Presta, M., Rusnati, M. (2005). {alpha}v{beta}3-integrin-dependent activation of focal adhesion kinase mediates NF-{kappa}B activation and motogenic activity by HIV-1 Tat in endothelial cells. J. Cell Sci. 118: 3949-3958 [Abstract] [Full Text]  
  • Rucci, N., DiGiacinto, C., Orru, L., Millimaggi, D., Baron, R., Teti, A. (2005). A novel protein kinase C {alpha}-dependent signal to ERK1/2 activated by {alpha}V{beta}3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells. J. Cell Sci. 118: 3263-3275 [Abstract] [Full Text]  
  • Heidkamp, M. C., Scully, B. T., Vijayan, K., Engman, S. J., Szotek, E. L., Samarel, A. M. (2005). PYK2 regulates SERCA2 gene expression in neonatal rat ventricular myocytes. Am. J. Physiol. Cell Physiol. 289: C471-C482 [Abstract] [Full Text]  
  • Golas, J. M., Lucas, J., Etienne, C., Golas, J., Discafani, C., Sridharan, L., Boghaert, E., Arndt, K., Ye, F., Boschelli, D. H., Li, F., Titsch, C., Huselton, C., Chaudhary, I., Boschelli, F. (2005). SKI-606, a Src/Abl Inhibitor with In vivo Activity in Colon Tumor Xenograft Models. Cancer Res. 65: 5358-5364 [Abstract] [Full Text]  
  • Cohen, L. A., Guan, J.-L. (2005). Residues within the First Subdomain of the FERM-like Domain in Focal Adhesion Kinase Are Important in Its Regulation. J. Biol. Chem. 280: 8197-8207 [Abstract] [Full Text]  
  • Brunton, V. G., Avizienyte, E., Fincham, V. J., Serrels, B., Metcalf, C. A. III, Sawyer, T. K., Frame, M. C. (2005). Identification of Src-Specific Phosphorylation Site on Focal Adhesion Kinase: Dissection of the Role of Src SH2 and Catalytic Functions and Their Consequences for Tumor Cell Behavior. Cancer Res. 65: 1335-1342 [Abstract] [Full Text]  
  • Lehoux, S., Esposito, B., Merval, R., Tedgui, A. (2005). Differential Regulation of Vascular Focal Adhesion Kinase by Steady Stretch and Pulsatility. Circulation 111: 643-649 [Abstract] [Full Text]  
  • Fonseca, P. M., Inoue, R. Y., Kobarg, C. B., Crosara-Alberto, D. P., Kobarg, J., Franchini, K. G. (2005). Targeting to C-Terminal Myosin Heavy Chain May Explain Mechanotransduction Involving Focal Adhesion Kinase in Cardiac Myocytes. Circ. Res. 96: 73-81 [Abstract] [Full Text]  
  • Westhoff, M. A., Serrels, B., Fincham, V. J., Frame, M. C., Carragher, N. O. (2004). Src-Mediated Phosphorylation of Focal Adhesion Kinase Couples Actin and Adhesion Dynamics to Survival Signaling. Mol. Cell. Biol. 24: 8113-8133 [Abstract] [Full Text]  
  • Read, R. D., Bach, E. A., Cagan, R. L. (2004). Drosophila C-Terminal Src Kinase Negatively Regulates Organ Growth and Cell Proliferation through Inhibition of the Src, Jun N-Terminal Kinase, and STAT Pathways. Mol. Cell. Biol. 24: 6676-6689 [Abstract] [Full Text]  
  • Lim, Y., Han, I., Jeon, J., Park, H., Bahk, Y.-Y., Oh, E.-S. (2004). Phosphorylation of Focal Adhesion Kinase at Tyrosine 861 Is Crucial for Ras Transformation of Fibroblasts. J. Biol. Chem. 279: 29060-29065 [Abstract] [Full Text]  
  • Giannone, G., Ronde, P., Gaire, M., Beaudouin, J., Haiech, J., Ellenberg, J., Takeda, K. (2004). Calcium Rises Locally Trigger Focal Adhesion Disassembly and Enhance Residency of Focal Adhesion Kinase at Focal Adhesions. J. Biol. Chem. 279: 28715-28723 [Abstract] [Full Text]  
  • Dunty, J. M., Gabarra-Niecko, V., King, M. L., Ceccarelli, D. F. J., Eck, M. J., Schaller, M. D. (2004). FERM Domain Interaction Promotes FAK Signaling. Mol. Cell. Biol. 24: 5353-5368 [Abstract] [Full Text]  
  • Armulik, A., Velling, T., Johansson, S. (2004). The Integrin {beta}1 Subunit Transmembrane Domain Regulates Phosphatidylinositol 3-Kinase-dependent Tyrosine Phosphorylation of Crk-associated Substrate. Mol. Biol. Cell 15: 2558-2567 [Abstract] [Full Text]  
  • Duxbury, M. S., Ito, H., Ashley, S. W., Whang, E. E. (2004). CEACAM6 Cross-linking Induces Caveolin-1-dependent, Src-mediated Focal Adhesion Kinase Phosphorylation in BxPC3 Pancreatic Adenocarcinoma Cells. J. Biol. Chem. 279: 23176-23182 [Abstract] [Full Text]  
  • Schmitter, T., Agerer, F., Peterson, L., Munzner, P., Hauck, C. R. (2004). Granulocyte CEACAM3 Is a Phagocytic Receptor of the Innate Immune System that Mediates Recognition and Elimination of Human-specific Pathogens. JEM 199: 35-46 [Abstract] [Full Text]  
  • Vial, D., Oliver, C., Jamur, M. C., Pastor, M. V. D., da Silva Trindade, E., Berenstein, E., Zhang, J., Siraganian, R. P. (2003). Alterations in Granule Matrix and Cell Surface of Focal Adhesion Kinase-Deficient Mast Cells. J. Immunol. 171: 6178-6186 [Abstract] [Full Text]  
  • Cooper, L. A., Shen, T.-L., Guan, J.-L. (2003). Regulation of Focal Adhesion Kinase by Its Amino-Terminal Domain through an Autoinhibitory Interaction. Mol. Cell. Biol. 23: 8030-8041 [Abstract] [Full Text]  
  • Acosta, J. J., Munoz, R. M., Gonzalez, L., Subtil-Rodriguez, A., Dominguez-Caceres, M. A., Garcia-Martinez, J. M., Calcabrini, A., Lazaro-Trueba, I., Martin-Perez, J. (2003). Src Mediates Prolactin-Dependent Proliferation of T47D and MCF7 Cells via the Activation of Focal Adhesion Kinase/Erk1/2 and Phosphatidylinositol 3-Kinase Pathways. Mol. Endocrinol. 17: 2268-2282 [Abstract] [Full Text]  
  • Heidkamp, M. C., Bayer, A. L., Scully, B. T., Eble, D. M., Samarel, A. M. (2003). Activation of focal adhesion kinase by protein kinase C{epsilon} in neonatal rat ventricular myocytes. Am. J. Physiol. Heart Circ. Physiol. 285: H1684-H1696 [Abstract] [Full Text]  
  • Sundberg, L. J., Galante, L. M., Bill, H. M., Mack, C. P., Taylor, J. M. (2003). An Endogenous Inhibitor of Focal Adhesion Kinase Blocks Rac1/JNK but Not Ras/ERK-dependent Signaling in Vascular Smooth Muscle Cells. J. Biol. Chem. 278: 29783-29791 [Abstract] [Full Text]  
  • Katz, B.-Z., Romer, L., Miyamoto, S., Volberg, T., Matsumoto, K., Cukierman, E., Geiger, B., Yamada, K. M. (2003). Targeting Membrane-localized Focal Adhesion Kinase to Focal Adhesions: ROLES OF TYROSINE PHOSPHORYLATION AND SRC FAMILY KINASES. J. Biol. Chem. 278: 29115-29120 [Abstract] [Full Text]  
  • Slack-Davis, J. K., Eblen, S. T., Zecevic, M., Boerner, S. A., Tarcsafalvi, A., Diaz, H. B., Marshall, M. S., Weber, M. J., Parsons, J. T., Catling, A. D. (2003). PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation. JCB 162: 281-291 [Abstract] [Full Text]  
  • Porter, M. J., Heidkamp, M. C., Scully, B. T., Patel, N., Martin, J. L., Samarel, A. M. (2003). Isoenzyme-selective regulation of SERCA2 gene expression by protein kinase C in neonatal rat ventricular myocytes. Am. J. Physiol. Cell Physiol. 285: C39-C47 [Abstract] [Full Text]  
  • Hunger-Glaser, I., Salazar, E. P., Sinnett-Smith, J., Rozengurt, E. (2003). Bombesin, Lysophosphatidic Acid, and Epidermal Growth Factor Rapidly Stimulate Focal Adhesion Kinase Phosphorylation at Ser-910: REQUIREMENT FOR ERK ACTIVATION. J. Biol. Chem. 278: 22631-22643 [Abstract] [Full Text]  
  • Pace, A., Garcia-Marin, L. J., Tapia, J. A., Bragado, M. J., Jensen, R. T. (2003). Phosphospecific Site Tyrosine Phosphorylation of p125FAK and Proline-rich Kinase 2 Is Differentially Regulated by Cholecystokinin Receptor Type A Activation in Pancreatic Acini. J. Biol. Chem. 278: 19008-19016 [Abstract] [Full Text]  
  • Yang, Y. C., Ma, Y. L., Chen, S. K., Wang, C. W., Lee, E. H. Y. (2003). Focal Adhesion Kinase Is Required, But Not Sufficient, for the Induction of Long-Term Potentiation in Dentate Gyrus Neurons In Vivo. J. Neurosci. 23: 4072-4080 [Abstract] [Full Text]  
  • Siu, M. K. Y., Mruk, D. D., Lee, W. M., Cheng, C. Y. (2003). Adhering Junction Dynamics in the Testis Are Regulated by an Interplay of {beta}1-Integrin and Focal Adhesion Complex-Associated Proteins. Endocrinology 144: 2141-2163 [Abstract] [Full Text]  
  • Parsons, J. T. (2003). Focal adhesion kinase: the first ten years. J. Cell Sci. 116: 1409-1416 [Abstract] [Full Text]  
  • Chan, P.-C., Liang, C.-C., Yu, K.-C., Chang, M.-C., Ho, W. L., Chen, B.-H., Chen, H.-C. (2002). Synergistic Effect of Focal Adhesion Kinase Overexpression and Hepatocyte Growth Factor Stimulation on Cell Transformation. J. Biol. Chem. 277: 50373-50379 [Abstract] [Full Text]  
  • Toutant, M., Costa, A., Studler, J.-M., Kadare, G., Carnaud, M., Girault, J.-A. (2002). Alternative Splicing Controls the Mechanisms of FAK Autophosphorylation. Mol. Cell. Biol. 22: 7731-7743 [Abstract] [Full Text]  
  • Dunty, J. M., Schaller, M. D. (2002). The N Termini of Focal Adhesion Kinase Family Members Regulate Substrate Phosphorylation, Localization, and Cell Morphology. J. Biol. Chem. 277: 45644-45654 [Abstract] [Full Text]  
  • Cheng, C. Y., Mruk, D. D. (2002). Cell Junction Dynamics in the Testis: Sertoli-Germ Cell Interactions and Male Contraceptive Development. Physiol. Rev. 82: 825-874 [Abstract] [Full Text]  
  • Wilcox-Adelman, S. A., Denhez, F., Goetinck, P. F. (2002). Syndecan-4 Modulates Focal Adhesion Kinase Phosphorylation. J. Biol. Chem. 277: 32970-32977 [Abstract] [Full Text]  
  • Heidkamp, M. C., Bayer, A. L., Kalina, J. A., Eble, D. M., Samarel, A. M. (2002). GFP-FRNK Disrupts Focal Adhesions and Induces Anoikis in Neonatal Rat Ventricular Myocytes. Circ. Res. 90: 1282-1289 [Abstract] [Full Text]  
  • Li, X., Regezi, J., Ross, F. P., Blystone, S., Ilic, D., Leong, S. P. L., Ramos, D. M. (2002). Integrin {alpha}v{beta}3 mediates K1735 murine melanoma cell motility in vivo and in vitro. J. Cell Sci. 114: 2665-2672 [Abstract] [Full Text]  
  • Chellaiah, M. A., Biswas, R. S., Yuen, D., Alvarez, U. M., Hruska, K. A. (2001). Phosphatidylinositol 3,4,5-Trisphosphate Directs Association of Src Homology 2-containing Signaling Proteins with Gelsolin. J. Biol. Chem. 276: 47434-47444 [Abstract] [Full Text]  
  • Schaeffer, M., Schneiderbauer, M., Weidler, S., Tavares, R., Warmuth, M., de Vos, G., Hallek, M. (2001). Signaling through a Novel Domain of gp130 Mediates Cell Proliferation and Activation of Hck and Erk Kinases. Mol. Cell. Biol. 21: 8068-8081 [Abstract] [Full Text]  
  • Shima, T., Okumura, N., Takao, T., Satomi, Y., Yagi, T., Okada, M., Nagai, K. (2001). Interaction of the SH2 Domain of Fyn with a Cytoskeletal Protein, beta -Adducin. J. Biol. Chem. 276: 42233-42240 [Abstract] [Full Text]  
  • You, L., Ebner, S., Kruse, F. E. (2001). Glial Cell-Derived Neurotrophic Factor (GDNF)-Induced Migration and Signal Transduction in Corneal Epithelial Cells. IOVS 42: 2496-2504 [Abstract] [Full Text]  
  • Hauck, C. R., Sieg, D. J., Hsia, D. A., Loftus, J. C., Gaarde, W. A., Monia, B. P., Schlaepfer, D. D. (2001). Inhibition of Focal Adhesion Kinase Expression or Activity Disrupts Epidermal Growth Factor-stimulated Signaling Promoting the Migration of Invasive Human Carcinoma Cells. Cancer Res. 61: 7079-7090 [Abstract] [Full Text]  
  • Pham, C. G., Harpf, A. E., Keller, R. S., Vu, H. T., Shai, S.-Y., Loftus, J. C., Ross, R. S. (2000). Striated muscle-specific beta 1D-integrin and FAK are involved in cardiac myocyte hypertrophic response pathway. Am. J. Physiol. Heart Circ. Physiol. 279: H2916-H2926 [Abstract] [Full Text]  
  • Reddy, M. A., Wass, C. A., Kim, K. S., Schlaepfer, D. D., Prasadarao, N. V. (2000). Involvement of Focal Adhesion Kinase in Escherichia coli Invasion of Human Brain Microvascular Endothelial Cells. Infect. Immun. 68: 6423-6430 [Abstract] [Full Text]  
  • Rogers, J. A., Cheng, H. Y., Smithgall, T. E. (2000). Src Homology 2 Domain Substitution Modulates the Kinase and Transforming Activities of the Fes Protein-Tyrosine Kinase. Cell Growth Differ. 11: 581-592 [Abstract] [Full Text]  
  • Govindarajan, G., Eble, D. M., Lucchesi, P. A., Samarel, A. M. (2000). Focal Adhesion Kinase Is Involved in Angiotensin II-Mediated Protein Synthesis in Cultured Vascular Smooth Muscle Cells. Circ. Res. 87: 710-716 [Abstract] [Full Text]  
  • Lavoie, J. N., Champagne, C., Gingras, M.-C., Robert, A. (2000). Adenovirus E4 Open Reading Frame 4-Induced Apoptosis Involves Dysregulation of Src Family Kinases. JCB 150: 1037-1056 [Abstract] [Full Text]  
  • Cooley, M. A., Broome, J. M., Ohngemach, C., Romer, L. H., Schaller, M. D. (2000). Paxillin Binding Is Not the Sole Determinant of Focal Adhesion Localization or Dominant-Negative Activity of Focal Adhesion Kinase/Focal Adhesion Kinase-related Nonkinase. Mol. Biol. Cell 11: 3247-3263 [Abstract] [Full Text]  
  • Ryu, H., Lee, J.-H., Kim, K. S., Jeong, S.-M., Kim, P.-H., Chung, H.-T. (2000). Regulation of Neutrophil Adhesion by Pituitary Growth Hormone Accompanies Tyrosine Phosphorylation of Jak2, p125FAK, and Paxillin. J. Immunol. 165: 2116-2123 [Abstract] [Full Text]  
  • Sheta, E. A., Harding, M. A., Conaway, M. R., Theodorescu, D. (2000). Focal Adhesion Kinase, Rap1, and Transcriptional Induction of Vascular Endothelial Growth Factor. JNCI J Natl Cancer Inst 92: 1065-1073 [Abstract] [Full Text]  
  • Eble, D. M., Strait, J. B., Govindarajan, G., Lou, J., Byron, K. L., Samarel, A. M. (2000). Endothelin-induced cardiac myocyte hypertrophy: role for focal adhesion kinase. Am. J. Physiol. Heart Circ. Physiol. 278: H1695-H1707 [Abstract] [Full Text]  
  • Borg, T. K, Goldsmith, E. C, Price, R., Carver, W., Terracio, L., Samarel, A. M (2000). Specialization at the Z line of cardiac myocytes. Cardiovasc Res 46: 277-285 [Full Text]  
  • Lai, J.-F., Kao, S.-C., Jiang, S.-T., Tang, M.-J., Chan, P.-C., Chen, H.-C. (2000). Involvement of Focal Adhesion Kinase in Hepatocyte Growth Factor-induced Scatter of Madin-Darby Canine Kidney Cells. J. Biol. Chem. 275: 7474-7480 [Abstract] [Full Text]  
  • Zhao, J, Zheng, C, Guan, J (2000). Pyk2 and FAK differentially regulate progression of the cell cycle. J. Cell Sci. 113: 3063-3072 [Abstract]  
  • Schaller, M. D., Hildebrand, J. D., Parsons, J. T. (1999). Complex Formation with Focal Adhesion Kinase: A Mechanism to Regulate Activity and Subcellular Localization of Src Kinases. Mol. Biol. Cell 10: 3489-3505 [Abstract] [Full Text]  
  • Chan, P.-C., Lai, J.-F., Cheng, C.-H., Tang, M.-J., Chiu, C.-C., Chen, H.-C. (1999). Suppression of Ultraviolet Irradiation-induced Apoptosis by Overexpression of Focal Adhesion Kinase in Madin-Darby Canine Kidney Cells. J. Biol. Chem. 274: 26901-26906 [Abstract] [Full Text]  
  • Asthagiri, A. R., Nelson, C. M., Horwitz, A. F., Lauffenburger, D. A. (1999). Quantitative Relationship among Integrin-Ligand Binding, Adhesion, and Signaling via Focal Adhesion Kinase and Extracellular Signal-regulated Kinase 2. J. Biol. Chem. 274: 27119-27127 [Abstract] [Full Text]  
  • Lock, P., Casagranda, F., Dunn, A. R. (1999). Independent SH2-binding Sites Mediate Interaction of Dok-related Protein with RasGTPase-activating Protein and Nck. J. Biol. Chem. 274: 22775-22784 [Abstract] [Full Text]  
  • Velarde, V., Ullian, M. E., Morinelli, T. A., Mayfield, R. K., Jaffa, A. A. (1999). Mechanisms of MAPK activation by bradykinin in vascular smooth muscle cells. Am. J. Physiol. Cell Physiol. 277: C253-C261 [Abstract] [Full Text]  
  • Owen, J. D., Ruest, P. J., Fry, D. W., Hanks, S. K. (1999). Induced Focal Adhesion Kinase (FAK) Expression in FAK-Null Cells Enhances Cell Spreading and Migration Requiring Both Auto- and Activation Loop Phosphorylation Sites and Inhibits Adhesion-Dependent Tyrosine Phosphorylation of Pyk2. Mol. Cell. Biol. 19: 4806-4818 [Abstract] [Full Text]  
  • Blaukat, A., Ivankovic-Dikic, I., Gronroos, E., Dolfi, F., Tokiwa, G., Vuori, K., Dikic, I. (1999). Adaptor Proteins Grb2 and Crk Couple Pyk2 with Activation of Specific Mitogen-activated Protein Kinase Cascades. J. Biol. Chem. 274: 14893-14901 [Abstract] [Full Text]  
  • Reiske, H. R., Kao, S.-C., Cary, L. A., Guan, J.-L., Lai, J.-F., Chen, H.-C. (1999). Requirement of Phosphatidylinositol 3-Kinase in Focal Adhesion Kinase-promoted Cell Migration. J. Biol. Chem. 274: 12361-12366 [Abstract] [Full Text]  
  • Oh, E.-S., Gu, H., Saxton, T. M., Timms, J. F., Hausdorff, S., Frevert, E. U., Kahn, B. B., Pawson, T., Neel, B. G., Thomas, S. M. (1999). Regulation of Early Events in Integrin Signaling by Protein Tyrosine Phosphatase SHP-2. Mol. Cell. Biol. 19: 3205-3215 [Abstract] [Full Text]  
  • Li, X., Dy, R. C., Cance, W. G., Graves, L. M., Earp, H. S. (1999). Interactions between Two Cytoskeleton-associated Tyrosine Kinases: Calcium-dependent Tyrosine Kinase and Focal Adhesion Tyrosine Kinase. J. Biol. Chem. 274: 8917-8924 [Abstract] [Full Text]  
  • Martin-Bermudo, M. D., Brown, N. H. (1999). Uncoupling integrin adhesion and signaling: the beta PS cytoplasmic domain is sufficient to regulate gene expression in the Drosophila embryo. Genes Dev. 13: 729-739 [Abstract] [Full Text]  
  • Angers-Loustau, A., Cote, J.-F., Charest, A., Dowbenko, D., Spencer, S., Lasky, L. A., Tremblay, M. L. (1999). Protein Tyrosine Phosphatase-PEST Regulates Focal Adhesion Disassembly, Migration, and Cytokinesis in Fibroblasts. JCB 144: 1019-1031 [Abstract] [Full Text]  
  • Garton, A. J., Tonks, N. K. (1999). Regulation of Fibroblast Motility by the Protein Tyrosine Phosphatase PTP-PEST. J. Biol. Chem. 274: 3811-3818 [Abstract] [Full Text]  
  • Ben-Dor, I., Bern, O., Tennenbaum, T. (1999). Cell Cycle-dependent Nuclear Accumulation of the p94fer Tyrosine Kinase Is Regulated by Its NH2 Terminus and Is Affected by Kinase Domain Integrity and ATP Binding. Cell Growth Differ. 10: 113-129 [Abstract] [Full Text]  
  • Takayama, Y., Tanaka, S., Nagai, K., Okada, M. (1999). Adenovirus-mediated Overexpression of C-terminal Src Kinase (Csk) in Type I Astrocytes Interferes with Cell Spreading and Attachment to Fibronectin. CORRELATION WITH TYROSINE PHOSPHORYLATIONS OF PAXILLIN AND FAK. J. Biol. Chem. 274: 2291-2297 [Abstract] [Full Text]  
  • Sieg, D., Hauck, C., Schlaepfer, D. (1999). Required role of focal adhesion kinase (FAK) for integrin-stimulated cell migration. J. Cell Sci. 112: 2677-2691 [Abstract]  
  • Zhao, J.-H., Reiske, H., Guan, J.-L. (1998). Regulation of the Cell Cycle by Focal Adhesion Kinase. JCB 143: 1997-2008 [Abstract] [Full Text]  
  • Kovacic, B., Damsky, C. H., Gardner, D. G. (1998). c-Src Activation Plays a Role in Endothelin-dependent Hypertrophy of the Cardiac Myocyte. J. Biol. Chem. 273: 35185-35193 [Abstract] [Full Text]  
  • Zhu, T., Goh, E. L. K., LeRoith, D., Lobie, P. E. (1998). Growth Hormone Stimulates the Formation of a Multiprotein Signaling Complex Involving p130Cas and CrkII. RESULTANT ACTIVATION OF c-Jun N-TERMINAL KINASE/STRESS-ACTIVATED PROTEIN KINASE (JNK/SAPK). J. Biol. Chem. 273: 33864-33875 [Abstract] [Full Text]  
  • Mainiero, F., Gismondi, A., Soriani, A., Cippitelli, M., Palmieri, G., Jacobelli, J., Piccoli, M., Frati, L., Santoni, A. (1998). Integrin-mediated Ras-Extracellular Regulated Kinase (ERK) Signaling Regulates Interferon {gamma} Production in Human Natural Killer Cells. JEM 188: 1267-1275 [Abstract] [Full Text]  
  • Chen, H.-C., Chan, P.-C., Tang, M.-J., Cheng, C.-H., Chang, T.-J. (1998). Tyrosine Phosphorylation of Focal Adhesion Kinase Stimulated by Hepatocyte Growth Factor Leads to Mitogen-activated Protein Kinase Activation. J. Biol. Chem. 273: 25777-25782 [Abstract] [Full Text]  
  • Aplin, A. E., Howe, A., Alahari, S. K., Juliano, R. L. (1998). Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins. Pharmacol. Rev. 50: 197-264 [Abstract] [Full Text]  
  • Tsuda, M., Matozaki, T., Fukunaga, K., Fujioka, Y., Imamoto, A., Noguchi, T., Takada, T., Yamao, T., Takeda, H., Ochi, F., Yamamoto, T., Kasuga, M. (1998). Integrin-mediated Tyrosine Phosphorylation of SHPS-1 and Its Association with SHP-2. ROLES OF Fak AND Src FAMILY KINASES. J. Biol. Chem. 273: 13223-13229 [Abstract] [Full Text]  
  • Chellaiah, M., Fitzgerald, C., Alvarez, U., Hruska, K. (1998). c-Src Is Required for Stimulation of Gelsolin-associated Phosphatidylinositol 3-Kinase. J. Biol. Chem. 273: 11908-11916 [Abstract] [Full Text]  
  • Schlaepfer, D. D., Jones, K. C., Hunter, T. (1998). Multiple Grb2-Mediated Integrin-Stimulated Signaling Pathways to ERK2/Mitogen-Activated Protein Kinase: Summation of Both c-Src- and Focal Adhesion Kinase-Initiated Tyrosine Phosphorylation Events. Mol. Cell. Biol. 18: 2571-2585 [Abstract] [Full Text]  
  • Fischer, R. S., Quinlan, M. P. (1998). Expression of the pRb-Binding Regions of E1A Enables Efficient Transformation of Primary Epithelial Cells by v-src. J. Virol. 72: 2815-2824 [Abstract] [Full Text]  
  • Cary, L. A., Han, D. C., Polte, T. R., Hanks, S. K., Guan, J.-L. (1998). Identification of p130Cas as a Mediator of Focal Adhesion Kinase-promoted Cell Migration. JCB 140: 211-221 [Abstract] [Full Text]  
  • Luttrell, L. M., Daaka, Y., Della Rocca, G. J., Lefkowitz, R. J. (1997). G Protein-coupled Receptors Mediate Two Functionally Distinct Pathways of Tyrosine Phosphorylation in Rat 1a Fibroblasts. Shc PHOSPHORYLATION AND RECEPTOR ENDOCYTOSIS CORRELATE WITH ACTIVATION OF Erk KINASES. J. Biol. Chem. 272: 31648-31656 [Abstract] [Full Text]  
  • Li, S., Kim, M., Hu, Y.-L., Jalali, S., Schlaepfer, D. D., Hunter, T., Chien, S., Shyy, J. Y-J. (1997). Fluid Shear Stress Activation of Focal Adhesion Kinase. LINKING TO MITOGEN-ACTIVATED PROTEIN KINASES. J. Biol. Chem. 272: 30455-30462 [Abstract] [Full Text]  
  • Hamawy, M. M., Swieter, M., Mergenhagen, S. E., Siraganian, R. P. (1997). Reconstitution of High Affinity IgE Receptor-mediated Secretion By Transfecting Protein Tyrosine Kinase pp125FAK. J. Biol. Chem. 272: 30498-30503 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»