This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murata, T. Articles by Ueda, H. Search for Related Content PubMed PubMed Citation Articles by Murata, T. Articles by Ueda, H.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Nov 1996, 6509-6515, Vol 16, No. 11
Copyright © 1996, American Society for Microbiology

Regulation of the EDG84A gene by FTZ-F1 during metamorphosis in Drosophila melanogaster

T Murata, Y Kageyama, S Hirose and H Ueda
Department of Genetics, The Graduate University for Advanced Studies, National Institute of Genetics, Shizuoka-ken, Japan.

The transcription factor FTZ-F1 is a member of the nuclear hormone receptor superfamily and is transiently expressed during the mid- and late prepupal periods in Drosophila melanogaster. A putative pupal cuticle gene, EDG84A, is expressed slightly following FTZ-F1 expression during the prepupal period and carries a strong FTZ-F1 binding site between bases 100 and 92 upstream of its transcription start site. In this study, EDG84A mRNA was found to be prematurely expressed upon heat induction of FTZ-F1 in prepupae carrying the heat shock promoter-FTZ-F1 cDNA fusion gene construct. Transgenic fly lines having the 0.8-kb region of the EDG84A promoter fused to lacZ expressed the reporter gene in a tissue- and stage-specific manner. Base substitutions in the FTZ- F1 binding site within the 0.8-kb promoter abolished expression of lacZ. These results strongly suggest that the EDG84A gene is a direct target of FTZ-F1. Deletion studies of the cis-regulatory region of the EDG84A gene revealed that space-specific expression in imaginal disc- derived epidermis is controlled by the region between bp -408 and -104 from the transcription start site. The region between bp -408 and -194 is necessary to repress expression in a posterior part of the body, while the region between bp -193 and -104 carries a positive element for activation in an anterior part of the body. These results suggest that FTZ-F1 governs expression of the EDG84A gene in conjunction with putative tissue-specific regulators.

This article has been cited by other articles:

• Agawa, Y., Sarhan, M., Kageyama, Y., Akagi, K., Takai, M., Hashiyama, K., Wada, T., Handa, H., Iwamatsu, A., Hirose, S., Ueda, H. (2007). Drosophila Blimp-1 Is a Transient Transcriptional Repressor That Controls Timing of the Ecdysone-Induced Developmental Pathway. Mol. Cell. Biol. 27: 8739-8747 [Abstract] [Full Text]  
• Suzuki, T., Kawasaki, H., Yu, R. T., Ueda, H., Umesono, K. (2001). Segmentation gene product Fushi tarazu is an LXXLL motif-dependent coactivator for orphan receptor FTZ-F1. Proc. Natl. Acad. Sci. USA 10.1073/pnas.221552998v1 [Abstract] [Full Text]  
• Yamada, M, Murata, T, Hirose, S, Lavorgna, G, Suzuki, E, Ueda, H (2000). Temporally restricted expression of transcription factor betaFTZ-F1: significance for embryogenesis, molting and metamorphosis in Drosophila melanogaster. Development 127: 5083-5092 [Abstract]  
• Li, T, Bender, M (2000). A conditional rescue system reveals essential functions for the ecdysone receptor (EcR) gene during molting and metamorphosis in Drosophila. Development 127: 2897-2905 [Abstract]  
• Takemaru, K.-i., Harashima, S., Ueda, H., Hirose, S. (1998). Yeast Coactivator MBF1 Mediates GCN4-Dependent Transcriptional Activation. Mol. Cell. Biol. 18: 4971-4976 [Abstract] [Full Text]  
• Takemaru, K.-i., Li, F.-Q., Ueda, H., Hirose, S. (1997). Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein. Proc. Natl. Acad. Sci. USA 94: 7251-7256 [Abstract] [Full Text]  
• Takeuchi, H., Chen, J.-H., O'Reilly, D. R., Turner, P. C., Rees, H. H. (2001). Regulation of Ecdysteroid Signaling: Cloning and Characterization of Ecdysone Oxidase. A NOVEL STEROID OXIDASE FROM THE COTTON LEAFWORM, SPODOPTERA LITTORALIS. J. Biol. Chem. 276: 26819-26828 [Abstract] [Full Text]  
• Suzuki, T., Kawasaki, H., Yu, R. T., Ueda, H., Umesono, K. (2001). Segmentation gene product Fushi tarazu is an LXXLL motif-dependent coactivator for orphan receptor FTZ-F1. Proc. Natl. Acad. Sci. USA 98: 12403-12408 [Abstract] [Full Text]