This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mathias, N.
Right arrow Articles by Goebl, M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mathias, N.
Right arrow Articles by Goebl, M. G.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Dec 1996, 6634-6643, Vol 16, No. 12
Copyright © 1996, American Society for Microbiology

Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S- phase transition and identifies a conserved family of proteins

N Mathias, SL Johnson, M Winey, AE Adams, L Goetsch, JR Pringle, B Byers and MG Goebl
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202-5122, USA.

Regulation of cell cycle progression occurs in part through the targeted degradation of both activating and inhibitory subunits of the cyclin-dependent kinases. During G1, CDC4, encoding a WD-40 repeat protein, and CDC34, encoding a ubiquitin-conjugating enzyme, are involved in the destruction of these regulators. Here we describe evidence indicating that CDC53 also is involved in this process. Mutations in CDC53 cause a phenotype indistinguishable from those of cdc4 and cdc34 mutations, numerous genetic interactions are seen between these genes, and the encoded proteins are found physically associated in vivo. Cdc53p defines a large family of proteins found in yeasts, nematodes, and humans whose molecular functions are uncharacterized. These results suggest a role for this family of proteins in regulating cell cycle proliferation through protein degradation.


This article has been cited by other articles:

  • Trunk, K., Gendron, P., Nantel, A., Lemieux, S., Roemer, T., Raymond, M. (2009). Depletion of the Cullin Cdc53p Induces Morphogenetic Changes in Candida albicans. Eukaryot Cell 8: 756-767 [Abstract] [Full Text]  
  • Jonkers, W., Rep, M. (2009). Lessons from Fungal F-Box Proteins. Eukaryot Cell 8: 677-695 [Full Text]  
  • Scaglione, K. M., Bansal, P. K., Deffenbaugh, A. E., Kiss, A., Moore, J. M., Korolev, S., Cocklin, R., Goebl, M., Kitagawa, K., Skowyra, D. (2007). SCF E3-Mediated Autoubiquitination Negatively Regulates Activity of Cdc34 E2 but Plays a Nonessential Role in the Catalytic Cycle In Vitro and In Vivo. Mol. Cell. Biol. 27: 5860-5870 [Abstract] [Full Text]  
  • Swaminathan, S., Kile, A. C., MacDonald, E. M., Koepp, D. M. (2007). Yra1 Is Required for S Phase Entry and Affects Dia2 Binding to Replication Origins. Mol. Cell. Biol. 27: 4674-4684 [Abstract] [Full Text]  
  • Anderson, V. E., Prudden, J., Prochnik, S., Giddings, T. H. Jr., Hardwick, K. G. (2007). Novel sfi1 Alleles Uncover Additional Functions for Sfi1p in Bipolar Spindle Assembly and Function. Mol. Biol. Cell 18: 2047-2056 [Abstract] [Full Text]  
  • Koepp, D. M., Kile, A. C., Swaminathan, S., Rodriguez-Rivera, V. (2006). The F-Box Protein Dia2 Regulates DNA Replication. Mol. Biol. Cell 17: 1540-1548 [Abstract] [Full Text]  
  • Jackson, L. P., Reed, S. I., Haase, S. B. (2006). Distinct mechanisms control the stability of the related s-phase cyclins clb5 and clb6.. Mol. Cell. Biol. 26: 2456-2466 [Abstract] [Full Text]  
  • Frigeri, L. G., Radabaugh, T. R., Haynes, P. A., Hildebrand, M. (2006). Identification of Proteins from a Cell Wall Fraction of the Diatom Thalassiosira pseudonana: Insights into Silica Structure Formation. Mol. Cell. Proteomics 5: 182-193 [Abstract] [Full Text]  
  • Furukawa, M., Xiong, Y. (2005). BTB Protein Keap1 Targets Antioxidant Transcription Factor Nrf2 for Ubiquitination by the Cullin 3-Roc1 Ligase. Mol. Cell. Biol. 25: 162-171 [Abstract] [Full Text]  
  • Bidlingmaier, S., Snyder, M. (2004). Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators. JCB 164: 207-218 [Abstract] [Full Text]  
  • Van Dort, C., Zhao, P., Parmelee, K., Capps, B., Poel, A., Listenberger, L., Kossoris, J., Wasilevich, B., Murrey, D., Clare, P., Burnatowska-Hledin, M. (2003). VACM-1, a cul-5 gene, inhibits cellular growth by a mechanism that involves MAPK and p53 signaling pathways. Am. J. Physiol. Cell Physiol. 285: C1386-C1396 [Abstract] [Full Text]  
  • Varelas, X., Ptak, C., Ellison, M. J. (2003). Cdc34 Self-Association Is Facilitated by Ubiquitin Thiolester Formation and Is Required for Its Catalytic Activity. Mol. Cell. Biol. 23: 5388-5400 [Abstract] [Full Text]  
  • Yoder, T. J., Pearson, C. G., Bloom, K., Davis, T. N. (2003). The Saccharomyces cerevisiae Spindle Pole Body Is a Dynamic Structure. Mol. Biol. Cell 14: 3494-3505 [Abstract] [Full Text]  
  • Wang, Y., Ge, Q., Houston, D., Thorner, J., Errede, B., Dohlman, H. G. (2003). Regulation of Ste7 Ubiquitination by Ste11 Phosphorylation and the Skp1-Cullin-F-box Complex. J. Biol. Chem. 278: 22284-22289 [Abstract] [Full Text]  
  • Michel, J. J., McCarville, J. F., Xiong, Y. (2003). A Role for Saccharomyces cerevisiae Cul8 Ubiquitin Ligase in Proper Anaphase Progression. J. Biol. Chem. 278: 22828-22837 [Abstract] [Full Text]  
  • Dixon, C., Brunson, L. E., Roy, M. M., Smothers, D., Sehorn, M. G., Mathias, N. (2003). Overproduction of Polypeptides Corresponding to the Amino Terminus of the F-Box Proteins Cdc4p and Met30p Inhibits Ubiquitin Ligase Activities of Their SCF Complexes. Eukaryot Cell 2: 123-133 [Abstract] [Full Text]  
  • Harper, J. W., Burton, J. L., Solomon, M. J. (2002). The anaphase-promoting complex: it's not just for mitosis any more. Genes Dev. 16: 2179-2206 [Full Text]  
  • Li, B., Ruiz, J. C., Chun, K. T. (2002). CUL-4A Is Critical for Early Embryonic Development. Mol. Cell. Biol. 22: 4997-5005 [Abstract] [Full Text]  
  • Linghu, B., Callis, J., Goebl, M. G. (2002). Rub1p Processing by Yuh1p Is Required for Wild-Type Levels of Rub1p Conjugation to Cdc53p. Eukaryot Cell 1: 491-494 [Abstract] [Full Text]  
  • Kepinski, S., Leyser, O. (2002). Ubiquitination and Auxin Signaling: A Degrading Story. Plant Cell 14: S81-95 [Full Text]  
  • Pioli, P. A., Rigby, W. F. C. (2001). The Von Hippel-Lindau Protein Interacts with Heteronuclear Ribonucleoprotein A2 and Regulates Its Expression. J. Biol. Chem. 276: 40346-40352 [Abstract] [Full Text]  
  • Nag, A., Bondar, T., Shiv, S., Raychaudhuri, P. (2001). The Xeroderma Pigmentosum Group E Gene Product DDB2 Is a Specific Target of Cullin 4A in Mammalian Cells. Mol. Cell. Biol. 21: 6738-6747 [Abstract] [Full Text]  
  • Yang, H., Kaelin, W. G. Jr. (2001). Molecular Pathogenesis of the Von Hippel-Lindau Hereditary Cancer Syndrome: Implications for Oxygen Sensing. Cell Growth Differ. 12: 447-455 [Full Text]  
  • Mohanty, S., Lee, S., Yadava, N., Dealy, M. J., Johnson, R. S., Firtel, R. A. (2001). Regulated protein degradation controls PKA function and cell-type differentiation in Dictyostelium. Genes Dev. 15: 1435-1448 [Abstract] [Full Text]  
  • Hinchcliffe, E. H., Sluder, G. (2001). "It Takes Two to Tango": understanding how centrosome duplication is regulated throughout the cell cycle. Genes Dev. 15: 1167-1181 [Full Text]  
  • Ohta, T., Xiong, Y. (2001). Phosphorylation- and SKP1-independent in Vitro Ubiquitination of E2F1 by Multiple ROC-Cullin Ligases. Cancer Res. 61: 1347-1353 [Abstract] [Full Text]  
  • Smothers, D. B., Kozubowski, L., Dixon, C., Goebl, M. G., Mathias, N. (2000). The Abundance of Met30p Limits SCFMet30p Complex Activity and Is Regulated by Methionine Availability. Mol. Cell. Biol. 20: 7845-7852 [Abstract] [Full Text]  
  • Furukawa, M., Zhang, Y., McCarville, J., Ohta, T., Xiong, Y. (2000). The CUL1 C-Terminal Sequence and ROC1 Are Required for Efficient Nuclear Accumulation, NEDD8 Modification, and Ubiquitin Ligase Activity of CUL1. Mol. Cell. Biol. 20: 8185-8197 [Abstract] [Full Text]  
  • Swanson, C., Ross, J., Jackson, P. K. (2000). Nuclear accumulation of cyclin E/Cdk2 triggers a concentration-dependent switch for the destruction of p27Xic1. Proc. Natl. Acad. Sci. USA 97: 7796-7801 [Abstract] [Full Text]  
  • Burnatowska-Hledin, M., Zhao, P., Capps, B., Poel, A., Parmelee, K., Mungall, C., Sharangpani, A., Listenberger, L. (2000). VACM-1, a cullin gene family member, regulates cellular signaling. Am. J. Physiol. Cell Physiol. 279: C266-C273 [Abstract] [Full Text]  
  • Meimoun, A., Holtzman, T., Weissman, Z., McBride, H. J., Stillman, D. J., Fink, G. R., Kornitzer, D. (2000). Degradation of the Transcription Factor Gcn4 Requires the Kinase Pho85 and the SCFCDC4 Ubiquitin-Ligase Complex. Mol. Biol. Cell 11: 915-927 [Abstract] [Full Text]  
  • Filippov, V, Filippova, M, Sehnal, F, Gill, S. (2000). Temporal and spatial expression of the cell-cycle regulator cul-1 in Drosophila and its stimulation by radiation-induced apoptosis. J. Exp. Biol. 203: 2747-2756 [Abstract]  
  • Shiyanov, P., Nag, A., Raychaudhuri, P. (1999). Cullin 4A Associates with the UV-damaged DNA-binding Protein DDB. J. Biol. Chem. 274: 35309-35312 [Abstract] [Full Text]  
  • Winston, J. T., Chu, C., Harper, J. W. (1999). Culprits in the degradation of cyclin E apprehended. Genes Dev. 13: 2751-2757 [Full Text]  
  • McMillan, J. N., Longtine, M. S., Sia, R. A. L., Theesfeld, C. L., Bardes, E. S. G., Pringle, J. R., Lew, D. J. (1999). The Morphogenesis Checkpoint in Saccharomyces cerevisiae: Cell Cycle Control of Swe1p Degradation by Hsl1p and Hsl7p. Mol. Cell. Biol. 19: 6929-6939 [Abstract] [Full Text]  
  • Freed, E., Lacey, K. R., Huie, P., Lyapina, S. A., Deshaies, R. J., Stearns, T., Jackson, P. K. (1999). Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle. Genes Dev. 13: 2242-2257 [Abstract] [Full Text]  
  • Galan, J.-M., Peter, M. (1999). Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanism. Proc. Natl. Acad. Sci. USA 96: 9124-9129 [Abstract] [Full Text]  
  • Liakopoulos, D., Busgen, T., Brychzy, A., Jentsch, S., Pause, A. (1999). Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function. Proc. Natl. Acad. Sci. USA 96: 5510-5515 [Abstract] [Full Text]  
  • Skowyra, D., Koepp, D. M., Kamura, T., Conrad, M. N., Conaway, R. C., Conaway, J. W., Elledge, S. J., Harper, J. W. (1999). Reconstitution of G1 Cyclin Ubiquitination with Complexes Containing SCFGrr1 and Rbx1. Science 284: 662-665 [Abstract] [Full Text]  
  • Mathias, N., Johnson, S., Byers, B., Goebl, M. (1999). The Abundance of Cell Cycle Regulatory Protein Cdc4p Is Controlled by Interactions between Its F Box and Skp1p. Mol. Cell. Biol. 19: 1759-1767 [Abstract] [Full Text]  
  • Iraqui, I., Vissers, S., Bernard, F., de Craene, J.-O., Boles, E., Urrestarazu, A., Andre, B. (1999). Amino Acid Signaling in Saccharomyces cerevisiae: a Permease-Like Sensor of External Amino Acids and F-Box Protein Grr1p Are Required for Transcriptional Induction of the AGP1 Gene, Which Encodes a Broad-Specificity Amino Acid Permease. Mol. Cell. Biol. 19: 989-1001 [Abstract] [Full Text]  
  • Nguyen, H., Gitig, D. M., Koff, A. (1999). Cell-Free Degradation of p27kip1, a G1 Cyclin-Dependent Kinase Inhibitor, Is Dependent on CDK2 Activity and the Proteasome. Mol. Cell. Biol. 19: 1190-1201 [Abstract] [Full Text]  
  • Lee, S., Neumann, M., Stearman, R., Stauber, R., Pause, A., Pavlakis, G. N., Klausner, R. D. (1999). Transcription-Dependent Nuclear-Cytoplasmic Trafficking Is Required for the Function of the von Hippel-Lindau Tumor Suppressor Protein. Mol. Cell. Biol. 19: 1486-1497 [Abstract] [Full Text]  
  • Burnatowska-Hledin, M., Lazdins, I. B., Listenberger, L., Zhao, P., Sharangpani, A., Folta, V., Card, B. (1999). VACM-1 receptor is specifically expressed in rabbit vascular endothelium and renal collecting tubule. Am. J. Physiol. Renal Physiol. 276: F199-F209 [Abstract] [Full Text]  
  • Park, M, Krause, M. (1999). Regulation of postembryonic G(1) cell cycle progression in Caenorhabditis elegans by a cyclin D/CDK-like complex. Development 126: 4849-4860 [Abstract]  
  • Yam, C. H., Ng, R. W. M., Yi Siu, W., Lau, A. W. S., Poon, R. Y. C. (1999). Regulation of Cyclin A-Cdk2 by SCF Component Skp1 and F-Box Protein Skp2. Mol. Cell. Biol. 19: 635-645 [Abstract] [Full Text]  
  • Mendenhall, M. D., Hodge, A. E. (1998). Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62: 1191-1243 [Abstract] [Full Text]  
  • Iliopoulos, O., Ohh, M., Kaelin, W. G. Jr. (1998). pVHL19 is a biologically active product of the von Hippel-Lindau gene arising from internal translation initiation. Proc. Natl. Acad. Sci. USA 95: 11661-11666 [Abstract] [Full Text]  
  • Du, M., Sansores-Garcia, L., Zu, Z., Wu, K. K.-y. (1998). Cloning and Expression Analysis of a Novel Salicylate Suppressible Gene, Hs-CUL-3, a Member of Cullin/Cdc53 Family. J. Biol. Chem. 273: 24289-24292 [Abstract] [Full Text]  
  • Kaiser, P., Sia, R. A.L., Bardes, E. G.S., Lew, D. J., Reed, S. I. (1998). Cdc34 and the F-box protein Met30 are required for degradation of the Cdk-inhibitory kinase Swe1. Genes Dev. 12: 2587-2597 [Abstract] [Full Text]  
  • Osaka, F., Kawasaki, H., Aida, N., Saeki, M., Chiba, T., Kawashima, S., Tanaka, K., Kato, S. (1998). A new NEDD8-ligating system for cullin-4A. Genes Dev. 12: 2263-2268 [Abstract] [Full Text]  
  • Jallepalli, P. V., Tien, D., Kelly, T. J. (1998). sud1+ targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast. Proc. Natl. Acad. Sci. USA 95: 8159-8164 [Abstract] [Full Text]  
  • Lyapina, S. A., Correll, C. C., Kipreos, E. T., Deshaies, R. J. (1998). Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein. Proc. Natl. Acad. Sci. USA 95: 7451-7456 [Abstract] [Full Text]  
  • Lammer, D., Mathias, N., Laplaza, J. M., Jiang, W., Liu, Y., Callis, J., Goebl, M., Estelle, M. (1998). Modification of yeast Cdc53p by the ubiquitin-related protein Rub1p affects function of the SCFCdc4 complex. Genes Dev. 12: 914-926 [Abstract] [Full Text]  
  • Patton, E. E., Willems, A. R., Sa, D., Kuras, L., Thomas, D., Craig, K. L., Tyers, M. (1998). Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast. Genes Dev. 12: 692-705 [Abstract] [Full Text]  
  • Zachariae, W., Shevchenko, A., Andrews, P. D., Ciosk, R., Galova, M., Stark, M. J., Mann, M., Nasmyth, K. (1998). Mass Spectrometric Analysis of the Anaphase-Promoting Complex from Yeast: Identification of a Subunit Related to Cullins. Science 279: 1216-1219 [Abstract] [Full Text]  
  • Yu, H., Peters, J., King, R. W., Page, A. M., Hieter, P., Kirschner, M. W. (1998). Identification of a Cullin Homology Region in a Subunit of the Anaphase-Promoting Complex. Science 279: 1219-1222 [Abstract] [Full Text]  
  • Mathias, N., Steussy, C. N., Goebl, M. G. (1998). An Essential Domain within Cdc34p Is Required for Binding to a Complex Containing Cdc4p and Cdc53p in Saccharomyces cerevisiae. J. Biol. Chem. 273: 4040-4045 [Abstract] [Full Text]  
  • Pause, A., Lee, S., Lonergan, K. M., Klausner, R. D. (1998). The von Hippel-Lindau tumor suppressor gene is required for cell cycle exit upon serum withdrawal. Proc. Natl. Acad. Sci. USA 95: 993-998 [Abstract] [Full Text]  
  • Hong, Y, Roy, R, Ambros, V (1998). Developmental regulation of a cyclin-dependent kinase inhibitor controls postembryonic cell cycle progression in Caenorhabditis elegans. Development 125: 3585-3597 [Abstract]  
  • Sanchez-Diaz, A, Gonzalez, I, Arellano, M, Moreno, S (1998). The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast. J. Cell Sci. 111: 843-851 [Abstract]  
  • Hubbard, E. J. A., Wu, G., Kitajewski, J., Greenwald, I. (1997). sel-10, a negative regulator of lin-12 activity in Caenorhabditis elegans, encodes a member of the CDC4 family of proteins. Genes Dev. 11: 3182-3193 [Abstract] [Full Text]  
  • Verma, R., Annan, R. S., Huddleston, M. J., Carr, S. A., Reynard, G., Deshaies, R. J. (1997). Phosphorylation of Sic1p by G1 Cdk Required for Its Degradation and Entry into S Phase. Science 278: 455-460 [Abstract] [Full Text]  
  • Yew, P. R., Kirschner, M. W. (1997). Proteolysis and DNA Replication: The CDC34 Requirement in the Xenopus Egg Cell Cycle. Science 277: 1672-1676 [Abstract] [Full Text]  
  • Kominami, K, Toda, T (1997). Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18.. Genes Dev. 11: 1548-1560 [Abstract]  
  • Pause, A., Lee, S., Worrell, R. A., Chen, D. Y. T., Burgess, W. H., Linehan, W. M., Klausner, R. D. (1997). The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins. Proc. Natl. Acad. Sci. USA 94: 2156-2161 [Abstract] [Full Text]  
  • King, R. W., Deshaies, R. J., Peters, J.-M., Kirschner, M. W. (1996). How Proteolysis Drives the Cell Cycle. Science 274: 1652-1659 [Abstract] [Full Text]  
  • Liu, W., Nichols, A. F., Graham, J. A., Dualan, R., Abbas, A., Linn, S. (2000). Nuclear Transport of Human DDB Protein Induced by Ultraviolet Light. J. Biol. Chem. 275: 21429-21434 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»