This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toda, T.
Right arrow Articles by Kuno, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toda, T.
Right arrow Articles by Kuno, T.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 12 1996, 6752-6764, Vol 16, No. 12
Copyright © 1996, American Society for Microbiology

The fission yeast pmk1+ gene encodes a novel mitogen-activated protein kinase homolog which regulates cell integrity and functions coordinately with the protein kinase C pathway

T Toda, S Dhut, G Superti-Furga, Y Gotoh, E Nishida, R Sugiura and T Kuno
Cell Regulation Laboratory, Imperial Cancer Research Fund, London, United Kingdom. toda@europa.lif.icnet.uk

We have isolated a gene, pmk1+, a third mitogen-activated protein kinase (MAPK) gene homolog from the fission yeast Schizosaccharomyces pombe. The predicted amino acid sequence shows the most homology (63 to 65% identity) to those of budding yeast Saccharomyces Mpk1 and Candida Mkc1. The Pmk1 protein contains phosphorylated tyrosines, and the level of tyrosine phosphorylation was increased in the dsp1 mutant which lacks an attenuating phosphatase for Pmk1. The level of tyrosine phosphorylation appears constant during hypotonic or heat shock treatment. The cells with pmk1 deleted (delta pmk1) are viable but show various defective phenotypes, including cell wall weakness, abnormal cell shape, a cytokinesis defect, and altered sensitivities to cations, such as hypersensitivity to potassium and resistance to sodium. Consistent with a high degree of conservation of amino acid sequence, multicopy plasmids containing the MPK1 gene rescued the defective phenotypes of the delta pmk1 mutant. The frog MAPK gene also suppressed the pmk1 disruptant. The results of genetic analysis indicated that Pmk1 lies on a novel MAPK pathway which does not overlap functionally with the other two MAPK pathways, the Spk1-dependent mating signal pathway and Sty1/Spc1/Phh1-dependent stress-sensing pathway. In Saccharomyces cerevisiae, Mpk1 is involved in cell wall integrity and functions downstream of the protein kinase C homolog. In contrast, in S. pombe, Pmk1 may not act in a linear manner with respect to fission yeast protein kinase C homologs. Interestingly, however, these two pathways are not independent; instead, they regulate cell integrity in a coordinate manner.


This article has been cited by other articles:

  • Fuchs, B. B., Mylonakis, E. (2009). Our Paths Might Cross: the Role of the Fungal Cell Wall Integrity Pathway in Stress Response and Cross Talk with Other Stress Response Pathways. Eukaryot Cell 8: 1616-1625 [Abstract] [Full Text]  
  • Nunez, A., Franco, A., Madrid, M., Soto, T., Vicente, J., Gacto, M., Cansado, J. (2009). Role for RACK1 Orthologue Cpc2 in the Modulation of Stress Response in Fission Yeast. Mol. Biol. Cell 20: 3996-4009 [Abstract] [Full Text]  
  • Ma, Y., Takeuchi, M., Sugiura, R., Sio, S. O., Kuno, T. (2009). Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast. GENES CELLS 14: 1015-1028 [Abstract] [Full Text]  
  • Fang, Y., Imagawa, K., Zhou, X., Kita, A., Sugiura, R., Jaiseng, W., Kuno, T. (2009). Pleiotropic phenotypes caused by an opal nonsense mutation in an essential gene encoding HMG-CoA reductase in fission yeast. GENES CELLS 14: 759-771 [Abstract] [Full Text]  
  • Satoh, R., Morita, T., Takada, H., Kita, A., Ishiwata, S., Doi, A., Hagihara, K., Taga, A., Matsumura, Y., Tohda, H., Sugiura, R. (2009). Role of the RNA-binding Protein Nrd1 and Pmk1 Mitogen-activated Protein Kinase in the Regulation of Myosin mRNA Stability in Fission Yeast. Mol. Biol. Cell 20: 2473-2485 [Abstract] [Full Text]  
  • Sharifmoghadam, M. R., Valdivieso, M.-H. (2009). The Fission Yeast SEL1 Domain Protein Cfh3p: A NOVEL REGULATOR OF THE GLUCAN SYNTHASE Bgs1p WHOSE FUNCTION IS MORE RELEVANT UNDER STRESS CONDITIONS. J. Biol. Chem. 284: 11070-11079 [Abstract] [Full Text]  
  • Garcia, P., Tajadura, V., Sanchez, Y. (2009). The Rho1p Exchange Factor Rgf1p Signals Upstream from the Pmk1 Mitogen-activated Protein Kinase Pathway in Fission Yeast. Mol. Biol. Cell 20: 721-731 [Abstract] [Full Text]  
  • Robertson, A. M., Hagan, I. M. (2008). Stress-regulated kinase pathways in the recovery of tip growth and microtubule dynamics following osmotic stress in S. pombe. J. Cell Sci. 121: 4055-4068 [Abstract] [Full Text]  
  • Park, G., Pan, S., Borkovich, K. A. (2008). Mitogen-Activated Protein Kinase Cascade Required for Regulation of Development and Secondary Metabolism in Neurospora crassa. Eukaryot Cell 7: 2113-2122 [Abstract] [Full Text]  
  • Fang, Y., Sugiura, R., Ma, Y., Yada-Matsushima, T., Umeno, H., Kuno, T. (2008). Cation Diffusion Facilitator Cis4 Is Implicated in Golgi Membrane Trafficking via Regulating Zinc Homeostasis in Fission Yeast. Mol. Biol. Cell 19: 1295-1303 [Abstract] [Full Text]  
  • Chen, D., Wilkinson, C. R.M., Watt, S., Penkett, C. J., Toone, W. M., Jones, N., Bahler, J. (2008). Multiple Pathways Differentially Regulate Global Oxidative Stress Responses in Fission Yeast. Mol. Biol. Cell 19: 308-317 [Abstract] [Full Text]  
  • Takada, H., Nishimura, M., Asayama, Y., Mannse, Y., Ishiwata, S., Kita, A., Doi, A., Nishida, A., Kai, N., Moriuchi, S., Tohda, H., Giga-Hama, Y., Kuno, T., Sugiura, R. (2007). Atf1 Is a Target of the Mitogen-activated Protein Kinase Pmk1 and Regulates Cell Integrity in Fission Yeast. Mol. Biol. Cell 18: 4794-4802 [Abstract] [Full Text]  
  • Tsutsumi, S., Sugiura, R., Ma, Y., Tokuoka, H., Ohta, K., Ohte, R., Noma, A., Suzuki, T., Kuno, T. (2007). Wobble Inosine tRNA Modification Is Essential to Cell Cycle Progression in G1/S and G2/M Transitions in Fission Yeast. J. Biol. Chem. 282: 33459-33465 [Abstract] [Full Text]  
  • Matsuo, Y., Fisher, E., Patton-Vogt, J., Marcus, S. (2007). Functional Characterization of the Fission Yeast Phosphatidylserine Synthase Gene, pps1, Reveals Novel Cellular Functions for Phosphatidylserine. Eukaryot Cell 6: 2092-2101 [Abstract] [Full Text]  
  • Madrid, M., Nunez, A., Soto, T., Vicente-Soler, J., Gacto, M., Cansado, J. (2007). Stress-activated Protein Kinase-mediated Down-Regulation of the Cell Integrity Pathway Mitogen-activated Protein Kinase Pmk1p by Protein Phosphatases. Mol. Biol. Cell 18: 4405-4419 [Abstract] [Full Text]  
  • Miyatake, M., Kuno, T., Kita, A., Katsura, K., Takegawa, K., Uno, S., Nabata, T., Sugiura, R. (2007). Valproic Acid Affects Membrane Trafficking and Cell-Wall Integrity in Fission Yeast. Genetics 175: 1695-1705 [Abstract] [Full Text]  
  • Ma, Y., Kuno, T., Kita, A., Asayama, Y., Sugiura, R. (2006). Rho2 Is a Target of the Farnesyltransferase Cpp1 and Acts Upstream of Pmk1 Mitogen-activated Protein Kinase Signaling in Fission Yeast. Mol. Biol. Cell 17: 5028-5037 [Abstract] [Full Text]  
  • Ma, Y., Kuno, T., Kita, A., Nabata, T., Uno, S., Sugiura, R. (2006). Genetic Evidence for Phospholipid-Mediated Regulation of the Rab GDP-Dissociation Inhibitor in Fission Yeast. Genetics 174: 1259-1271 [Abstract] [Full Text]  
  • Deng, L., Sugiura, R., Takeuchi, M., Suzuki, M., Ebina, H., Takami, T., Koike, A., Iba, S., Kuno, T. (2006). Real-Time Monitoring of Calcineurin Activity in Living Cells: Evidence for Two Distinct Ca2+-dependent Pathways in Fission Yeast. Mol. Biol. Cell 17: 4790-4800 [Abstract] [Full Text]  
  • Jin, Q.-W., Zhou, M., Bimbo, A., Balasubramanian, M. K., McCollum, D. (2006). A Role for the Septation Initiation Network in Septum Assembly Revealed by Genetic Analysis of sid2-250 Suppressors. Genetics 172: 2101-2112 [Abstract] [Full Text]  
  • He, Y., Sugiura, R., Ma, Y., Kita, A., Deng, L., Takegawa, K., Matsuoka, K., Shuntoh, H., Kuno, T. (2006). Genetic and functional interaction between Ryh1 and Ypt3: two Rab GTPases that function in S. pombe secretory pathway. GENES CELLS 11: 207-221 [Abstract] [Full Text]  
  • Madrid, M., Soto, T., Khong, H. K., Franco, A., Vicente, J., Perez, P., Gacto, M., Cansado, J. (2006). Stress-induced Response, Localization, and Regulation of the Pmk1 Cell Integrity Pathway in Schizosaccharomyces pombe. J. Biol. Chem. 281: 2033-2043 [Abstract] [Full Text]  
  • Morrell-Falvey, J. L., Ren, L., Feoktistova, A., Haese, G. D., Gould, K. L. (2005). Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p. J. Cell Sci. 118: 5563-5573 [Abstract] [Full Text]  
  • Santos, B., Martin-Cuadrado, A. B., Vazquez de Aldana, C. R., del Rey, F., Perez, P. (2005). Rho4 GTPase Is Involved in Secretion of Glucanases during Fission Yeast Cytokinesis. Eukaryot Cell 4: 1639-1645 [Abstract] [Full Text]  
  • Deng, L., Sugiura, R., Ohta, K., Tada, K., Suzuki, M., Hirata, M., Nakamura, S.-i., Shuntoh, H., Kuno, T. (2005). Phosphatidylinositol-4-phosphate 5-Kinase Regulates Fission Yeast Cell Integrity through a Phospholipase C-mediated Protein Kinase C-independent Pathway. J. Biol. Chem. 280: 27561-27568 [Abstract] [Full Text]  
  • Bimbo, A., Jia, Y., Poh, S. L., Karuturi, R. K. M., den Elzen, N., Peng, X., Zheng, L., O'Connell, M., Liu, E. T., Balasubramanian, M. K., Liu, J. (2005). Systematic Deletion Analysis of Fission Yeast Protein Kinases. Eukaryot Cell 4: 799-813 [Abstract] [Full Text]  
  • Sio, S. O., Suehiro, T., Sugiura, R., Takeuchi, M., Mukai, H., Kuno, T. (2005). The Role of the Regulatory Subunit of Fission Yeast Calcineurin for in Vivo Activity and Its Relevance to FK506 Sensitivity. J. Biol. Chem. 280: 12231-12238 [Abstract] [Full Text]  
  • Alonso-Nunez, M. L., An, H., Martin-Cuadrado, A. B., Mehta, S., Petit, C., Sipiczki, M., del Rey, F., Gould, K. L., Vazquez de Aldana, C. R. (2005). Ace2p Controls the Expression of Genes Required for Cell Separation in Schizosaccharomyces pombe. Mol. Biol. Cell 16: 2003-2017 [Abstract] [Full Text]  
  • Kita, A., Sugiura, R., Shoji, H., He, Y., Deng, L., Lu, Y., Sio, S. O., Takegawa, K., Sakaue, M., Shuntoh, H., Kuno, T. (2004). Loss of Apm1, the {micro}1 Subunit of the Clathrin-Associated Adaptor-Protein-1 Complex, Causes Distinct Phenotypes and Synthetic Lethality with Calcineurin Deletion in Fission Yeast. Mol. Biol. Cell 15: 2920-2931 [Abstract] [Full Text]  
  • Ogiso, Y., Sugiura, R., Kamo, T., Yanagiya, S., Lu, Y., Okazaki, K., Shuntoh, H., Kuno, T. (2004). Lub1 Participates in Ubiquitin Homeostasis and Stress Response via Maintenance of Cellular Ubiquitin Contents in Fission Yeast. Mol. Cell. Biol. 24: 2324-2331 [Abstract] [Full Text]  
  • Maeda, T., Sugiura, R., Kita, A., Saito, M., Deng, L., He, Y., Lu, Y., Fujita, Y., Takegawa, K., Shuntoh, H., Kuno, T. (2004). Pmr1, a P-type ATPase, and Pdt1, an Nramp homologue, cooperatively regulate cell morphogenesis in fission yeast: The importance of Mn2+ homeostasis. GENES CELLS 9: 71-82 [Abstract] [Full Text]  
  • Hirayama, S., Sugiura, R., Lu, Y., Maeda, T., Kawagishi, K., Yokoyama, M., Tohda, H., Giga-Hama, Y., Shuntoh, H., Kuno, T. (2003). Zinc Finger Protein Prz1 Regulates Ca2+ but Not Cl- Homeostasis in Fission Yeast. IDENTIFICATION OF DISTINCT BRANCHES OF CALCINEURIN SIGNALING PATHWAY IN FISSION YEAST. J. Biol. Chem. 278: 18078-18084 [Abstract] [Full Text]  
  • Berlin, A., Paoletti, A., Chang, F. (2003). Mid2p stabilizes septin rings during cytokinesis in fission yeast. JCB 160: 1083-1092 [Abstract] [Full Text]  
  • Tasto, J. J., Morrell, J. L., Gould, K. L. (2003). An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation. JCB 160: 1093-1103 [Abstract] [Full Text]  
  • Cheng, H., Sugiura, R., Wu, W., Fujita, M., Lu, Y., Sio, S. O., Kawai, R., Takegawa, K., Shuntoh, H., Kuno, T. (2002). Role of the Rab GTP-Binding Protein Ypt3 in the Fission Yeast Exocytic Pathway and Its Connection to Calcineurin Function. Mol. Biol. Cell 13: 2963-2976 [Abstract] [Full Text]  
  • Fujita, M., Sugiura, R., Lu, Y., Xu, L., Xia, Y., Shuntoh, H., Kuno, T. (2002). Genetic Interaction Between Calcineurin and Type 2 Myosin and Their Involvement in the Regulation of Cytokinesis and Chloride Ion Homeostasis in Fission Yeast. Genetics 161: 971-981 [Abstract] [Full Text]  
  • Calonge, T. M., Nakano, K., Arellano, M., Arai, R., Katayama, S., Toda, T., Mabuchi, I., Perez, P. (2000). Schizosaccharomyces pombe Rho2p GTPase Regulates Cell Wall alpha -Glucan Biosynthesis through the Protein Kinase Pck2p. Mol. Biol. Cell 11: 4393-4401 [Abstract] [Full Text]  
  • Bandyopadhyay, A., Matsumoto, T., Maitra, U. (2000). Fission Yeast Int6 Is Not Essential for Global Translation Initiation, but Deletion of int6+ Causes Hypersensitivity to Caffeine and Affects Spore Formation. Mol. Biol. Cell 11: 4005-4018 [Abstract] [Full Text]  
  • Miyamoto, R., Sugiura, R., Kamitani, S., Yada, T., Lu, Y., Sio, S. O., Asakura, M., Matsuhisa, A., Shuntoh, H., Kuno, T. (2000). Tol1, a Fission Yeast Phosphomonoesterase, Is an In Vivo Target of Lithium, and Its Deletion Leads to Sulfite Auxotrophy. J. Bacteriol. 182: 3619-3625 [Abstract] [Full Text]  
  • Loewith, R, Hubberstey, A, Young, D (2000). Skh1, the MEK component of the mkh1 signaling pathway in Schizosaccharomyces pombe. J. Cell Sci. 113: 153-160 [Abstract]  
  • Toya, M., Iino, Y., Yamamoto, M. (1999). Fission Yeast Pob1p, Which Is Homologous to Budding Yeast Boi Proteins and Exhibits Subcellular Localization Close to Actin Patches, Is Essential for Cell Elongation and Separation. Mol. Biol. Cell 10: 2745-2757 [Abstract] [Full Text]  
  • Katayama, S., Hirata, D., Arellano, M., Perez, P., Toda, T. (1999). Fission Yeast {alpha}-Glucan Synthase Mok1 Requires the Actin Cytoskeleton to Localize the Sites of Growth and Plays an Essential Role in Cell Morphogenesis Downstream of Protein Kinase C Function. JCB 144: 1173-1186 [Abstract] [Full Text]  
  • Arellano, M, Valdivieso, M., Calonge, T., Coll, P., Duran, A, Perez, P (1999). Schizosaccharomyces pombe protein kinase C homologues, pck1p and pck2p, are targets of rho1p and rho2p and differentially regulate cell integrity. J. Cell Sci. 112: 3569-3578 [Abstract]  
  • WIDMANN, C., GIBSON, S., JARPE, M. B., JOHNSON, G. L. (1999). Mitogen-Activated Protein Kinase: Conservation of a Three-Kinase Module From Yeast to Human. Physiol. Rev. 79: 143-180 [Abstract] [Full Text]  
  • Xu, J.-R., Staiger, C. J., Hamer, J. E. (1998). Inactivation of the mitogen-activated protein kinase Mps1 from the rice blast fungus prevents penetration of host cells but allows activation of plant defense responses. Proc. Natl. Acad. Sci. USA 95: 12713-12718 [Abstract] [Full Text]  
  • Zhang, Y., Sugiura, R., Lu, Y., Asami, M., Maeda, T., Itoh, T., Takenawa, T., Shuntoh, H., Kuno, T. (2000). Phosphatidylinositol 4-Phosphate 5-Kinase Its3 and Calcineurin Ppb1 Coordinately Regulate Cytokinesis in Fission Yeast. J. Biol. Chem. 275: 35600-35606 [Abstract] [Full Text]  
  • Murray, J. M., Johnson, D. I. (2001). The Cdc42p GTPase and Its Regulators Nrf1p and Scd1p Are Involved in Endocytic Trafficking in the Fission Yeast Schizosaccharomyces pombe. J. Biol. Chem. 276: 3004-3009 [Abstract] [Full Text]  
  • Yada, T., Sugiura, R., Kita, A., Itoh, Y., Lu, Y., Hong, Y., Kinoshita, T., Shuntoh, H., Kuno, T. (2001). Its8, a Fission Yeast Homolog of Mcd4 and Pig-n, Is Involved in GPI Anchor Synthesis and Shares an Essential Function with Calcineurin in Cytokinesis. J. Biol. Chem. 276: 13579-13586 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»