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Mol. Cell. Biol., Mar 1996, 1094-1102, Vol 16, No. 3
Copyright © 1996, American Society for Microbiology

Phosphorylation-dependent formation of a quaternary complex at the c- fos SRE

H Gille, M Kortenjann, T Strahl and PE Shaw
Spemann Laboratories, Max-Planck-Institut fur Immunbiologie, Freiburg,Germany.

The rapid and transient induction of the human proto-oncogene c-fos in response to a variety of stimuli depends on the serum responses element (SRE). In vivo footprinting experiments show that this promoter element is bound by a multicomponent complex including the serum response factor (SRF) and a ternary complex factor such as Elk-1. SRF is thought to recruit a ternary complex factor monomer into an asymmetric complex. In this report, we describe a quaternary complex over the SRE which, in addition to an SRF dimer, contains two Elk-1 molecules. Its formation at the SRE is strictly dependent on phosphorylation of S-383 in the Elk- 1 regulatory domain and appears to involve a weak intermolecular association between the two Elk-1 molecules. The influence of mutations in Elk-1 on quaternary complex formation in vitro correlates with their effect on the induction of c-fos reporter expression in response to mitogenic stimuli in vivo.

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