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Mol. Cell. Biol., 03 1996, 868-876, Vol 16, No. 3
Copyright © 1996, American Society for Microbiology

3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region [published erratum appears in Mol Cell Biol 1996 Apr;16(4):1880]

G Sithanandam, F Latif, FM Duh, R Bernal, U Smola, H Li, I Kuzmin, V Wixler, L Geil and S Shrestha
Biological Carcinogenesis and Development Program, PRI/DynCorp, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.

NotI linking clones, localized to the human chromosome 3p21.3 region and homozygously deleted in small cell lung cancer cell lines NCI-H740 and NCI-H1450, were used to search for a putative tumor suppressor gene(s). One of these clones, NL1G210, detected a 2.5-kb mRNA in all examined human tissues, expression being especially high in the heart and skeletal muscle. Two overlapping cDNA clones containing the entire open reading frame were isolated from a human heart cDNA library and fully characterized. Computer analysis and a search of the GenBank database to reveal high sequence identity of the product of this gene to serine-threonine kinases, especially to mitogen-activated protein kinase-activated protein kinase 2, a recently described substrate of mitogen-activated kinases. Sequence identitiy was 72% at the nucleotide level and 75% at the amino acid level, strongly suggesting that this protein is a serine-threonine kinase. Here we demonstrate that the new gene, referred to as 3pK (for chromosome 3p kinase), in fact encodes a mitogen-activated protein kinase-regulated protein serine-threonine kinase with a novel substrate specificity.

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