This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wade, P. A. Articles by Jaehning, J. A. Search for Related Content PubMed PubMed Citation Articles by Wade, P. A. Articles by Jaehning, J. A.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 04 1996, 1641-1648, Vol 16, No. 4
Copyright © 1996, American Society for Microbiology

Transcriptional corepression in vitro: a Mot1p-associated form of TATA- binding protein is required for repression by Leu3p

PA Wade and JA Jaehning
Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, 80262, USA.

Signals from transcriptional activators to the general mRNA transcription apparatus are communicated by factors associated with RNA polymerase II or the TATA-binding protein (TBP). Currently, little is known about how gene-specific transcription repressors communicate with RNA polymerase II. We have analyzed the requirements for repression by the saccharomyces cerevisiae Leu3 protein (Leu3p) in a reconstituted transcription system. We have identified a complex form of TBP which is required for communication of the repressing signal. This TFIID-like complex contains a known TBP-associated protein, Mot1p, which has been implicated in the repression of a subset of yeast genes by genetic analysis. Leu3p-dependent repression can be reconstituted with purified Mot1p and recombinant TBP. In addition, a mutation in the Mot1 gene leads to partial derepression of the Leu3p-dependent LEU2 promoter. These in vivo and in vitro observations define a role for Mot1p as a transcriptional corepressor.

This article has been cited by other articles:

• Kodgire, P., Dixit, M., Rao, K. K. (2006). ScoC and SinR Negatively Regulate epr by Corepression in Bacillus subtilis.. J. Bacteriol. 188: 6425-6428 [Abstract] [Full Text]  
• Kohlhaw, G. B. (2003). Leucine Biosynthesis in Fungi: Entering Metabolism through the Back Door. Microbiol. Mol. Biol. Rev. 67: 1-15 [Abstract] [Full Text]  
• Brzeski, J., Jerzmanowski, A. (2003). Deficient in DNA Methylation 1 (DDM1) Defines a Novel Family of Chromatin-remodeling Factors. J. Biol. Chem. 278: 823-828 [Abstract] [Full Text]  
• Geisberg, J. V., Moqtaderi, Z., Kuras, L., Struhl, K. (2002). Mot1 Associates with Transcriptionally Active Promoters and Inhibits Association of NC2 in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 8122-8134 [Abstract] [Full Text]  
• Dasgupta, A., Darst, R. P., Martin, K. J., Afshari, C. A., Auble, D. T. (2002). Mot1 activates and represses transcription by direct, ATPase-dependent mechanisms. Proc. Natl. Acad. Sci. USA 99: 2666-2671 [Abstract] [Full Text]  
• Oda, T., Kayukawa, K., Hagiwara, H., Yudate, H. T., Masuho, Y., Murakami, Y., Tamura, T.-a., Muramatsu, M.-a. (2000). A Novel TATA-Binding Protein-Binding Protein, ABT1, Activates Basal Transcription and Has a Yeast Homolog That Is Essential for Growth. Mol. Cell. Biol. 20: 1407-1418 [Abstract] [Full Text]  
• Crowe, A. J., Sang, L., Li, K. K., Lee, K. C., Spear, B. T., Barton, M. C. (1999). Hepatocyte Nuclear Factor 3 Relieves Chromatin-mediated Repression of the alpha -Fetoprotein Gene. J. Biol. Chem. 274: 25113-25120 [Abstract] [Full Text]  
• Wang, D., Zheng, F., Holmberg, S., Kohlhaw, G. B. (1999). Yeast Transcriptional Regulator Leu3p. SELF-MASKING, SPECIFICITY OF MASKING, AND EVIDENCE FOR REGULATION BY THE INTRACELLULAR LEVEL OF Leu3p. J. Biol. Chem. 274: 19017-19024 [Abstract] [Full Text]  
• Lee, T. I., Wyrick, J. J., Koh, S. S., Jennings, E. G., Gadbois, E. L., Young, R. A. (1998). Interplay of Positive and Negative Regulators in Transcription Initiation by RNA Polymerase II Holoenzyme. Mol. Cell. Biol. 18: 4455-4462 [Abstract] [Full Text]  
• Hampsey, M. (1998). Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery. Microbiol. Mol. Biol. Rev. 62: 465-503 [Abstract] [Full Text]  
• Lee, T. I., Young, R. A. (1998). Regulation of gene expression by TBP-associated proteins. Genes Dev. 12: 1398-1408 [Full Text]  
• Chicca, J. J. II, Auble, D. T., Pugh, B. F. (1998). Cloning and Biochemical Characterization of TAF-172, a Human Homolog of Yeast Mot1. Mol. Cell. Biol. 18: 1701-1710 [Abstract] [Full Text]  
• Kokubo, T., Swanson, M. J., Nishikawa, J.-i., Hinnebusch, A. G., Nakatani, Y. (1998). The Yeast TAF145 Inhibitory Domain and TFIIA Competitively Bind to TATA-Binding Protein. Mol. Cell. Biol. 18: 1003-1012 [Abstract] [Full Text]  
• van der Knaap, J. A., Borst, J. W., van der Vliet, P. C., Gentz, R., Timmers, H. Th. M. (1997). Cloning of the cDNA for the TATA-binding protein-associated factorII170 subunit of transcription factor B-TFIID reveals homology to global transcription regulators in yeast and Drosophila. Proc. Natl. Acad. Sci. USA 94: 11827-11832 [Abstract] [Full Text]  
• Wang, D., Hu, Y., Zheng, F., Zhou, K., Kohlhaw, G. B. (1997). Evidence That Intramolecular Interactions Are Involved in Masking the Activation Domain of Transcriptional Activator Leu3p. J. Biol. Chem. 272: 19383-19392 [Abstract] [Full Text]  
• Adamkewicz, J. I., Mueller, C. G. F., Hansen, K. E., Prud'homme, W. A., Thorner, J. (2000). Purification and Enzymic Properties of Mot1 ATPase, a Regulator of Basal Transcription in the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 275: 21158-21168 [Abstract] [Full Text]  
• Adamkewicz, J. I., Hansen, K. E., Prud'homme, W. A., Davis, J. L., Thorner, J. (2001). High Affinity Interaction of Yeast Transcriptional Regulator, Mot1, with TATA Box-binding Protein (TBP). J. Biol. Chem. 276: 11883-11894 [Abstract] [Full Text]