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Mol. Cell. Biol., May 1996, 1896-1908, Vol 16, No. 5
Copyright © 1996, American Society for Microbiology

A Drosophila homolog of the Rac- and Cdc42-activated serine/threonine kinase PAK is a potential focal adhesion and focal complex protein that colocalizes with dynamic actin structures

N Harden, J Lee, HY Loh, YM Ong, I Tan, T Leung, E Manser and L Lim
Glaxo-IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore.

Changes in cell morphology are essential in the development of a multicellular organism. The regulation of the cytoskeleton by the Rho subfamily of small GTP-binding proteins is an important determinant of cell shape. The Rho subfamily has been shown to participate in a variety of morphogenetic processes during Drosophila melanogaster development. We describe here a Drosophila homolog, DPAK, of the serine/threonine kinase PAK, a protein which is a target of the Rho subfamily proteins Rac and Cdc42. Rac, Cdc42, and PAK have previously been implicated in signaling by c-Jun amino-terminal kinases. DPAK bound to activated (GTP-bound) Drosophila Rac (DRacA) and Drosophila Cdc42. Similarities in the distributions of DPAK, integrin, and phosphotyrosine suggested an association of DPAK with focal adhesions and Cdc42- and Rac-induced focal adhesion-like focal complexes. DPAK was elevated in the leading edge of epidermal cells, whose morphological changes drive dorsal closure of the embryo. We have previously shown that the accumulation of cytoskeletal elements initiating cell shape changes in these cells could be inhibited by expression of a dominant-negative DRacA transgene. We show that leading- edge epidermal cells flanking segment borders, which express particularly large amounts of DPAK, undergo transient losses of cytoskeletal structures during dorsal closure. We propose that DPAK may be regulating the cytoskeleton through its association with focal adhesions and focal complexes and may be participating with DRacA in a c-Jun amino-terminal kinase signaling pathway recently demonstrated to be required for dorsal closure.


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  • Glise, B, Noselli, S (1997). Coupling of Jun amino-terminal kinase and Decapentaplegic signaling pathways in Drosophila morphogenesis.. Genes Dev. 11: 1738-1747 [Abstract]  
  • Wu, C., Lee, S.-F., Furmaniak-Kazmierczak, E., Cote, G. P., Thomas, D. Y., Leberer, E. (1996). Activation of Myosin-I by Members of the Ste20p Protein Kinase Family. J. Biol. Chem. 271: 31787-31790 [Abstract] [Full Text]  
  • Sluss, H K, Han, Z, Barrett, T, Davis, R J, Ip, Y T (1996). A JNK signal transduction pathway that mediates morphogenesis and an immune response in Drosophila.. Genes Dev. 10: 2745-2758 [Abstract]  
  • Chen, W., Chen, S., Yap, S. F., Lim, L. (1996). The Caenorhabditis elegans p21-activated Kinase (CePAK) Colocalizes with CeRac1 and CDC42Ce at Hypodermal Cell Boundaries during Embryo Elongation. J. Biol. Chem. 271: 26362-26368 [Abstract] [Full Text]  
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  • Raymond, K., Bergeret, E., Dagher, M.-C., Breton, R., Griffin-Shea, R., Fauvarque, M.-O. (2001). The Rac GTPase-activating Protein RotundRacGAP Interferes with Drac1 and Dcdc42 Signalling in Drosophila melanogaster. J. Biol. Chem. 276: 35909-35916 [Abstract] [Full Text]  


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