This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dick, T.
Right arrow Articles by Chia, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dick, T.
Right arrow Articles by Chia, W.

 Previous Article  |  Next Article 

Mol. Cell. Biol., 05 1996, 1966-1977, Vol 16, No. 5
Copyright © 1996, American Society for Microbiology

Cytoplasmic dynein (ddlc1) mutations cause morphogenetic defects and apoptotic cell death in Drosophila melanogaster

T Dick, K Ray, HK Salz and W Chia
Institute of Molecular and Cell Biology, National University of Singapore.

We report the molecular and genetic characterization of the cytoplasmic dynein light-chain gene, ddlc1, from Drosophila melanogaster. ddlc1 encodes the first cytoplasmic dynein light chain identified, and its genetic analysis represents the first in vivo characterization of cytoplasmic dynein function in higher eucaryotes. The ddlc1 gene maps to 4E1-2 and encodes an 89-amino-acid polypeptide with a high similarity to the axonemal 8-kDa outer-arm dynein light chain from Chlamydomonas flagella. Developmental Northern (RNA) blot analysis and ovary and embryo RNA in situ hybridizations indicate that the ddlc1 gene is expressed ubiquitously. Anti-DDLC1 antibody analyses show that the DDLC1 protein is localized in the cytoplasm. P-element-induced partial-loss-of-function mutations cause pleiotropic morphogenetic defects in bristle and wing development, as well as in oogenesis, and hence result in female sterility. The morphological abnormalities found in the ovaries are always associated with a loss of cellular shape and structure, as visualized by a disorganization of the actin cytoskeleton. Total-loss-of-function mutations cause lethality. A large proportion of mutant animals degenerate during embryogenesis, and the dying cells show morphological changes characteristic of apoptosis, namely, cell and nuclear condensation and fragmentation, as well as DNA degradation. Cloning of the human homolog of the ddlc1 gene, hdlc1, demonstrates that the dynein light-chain 1 is highly conserved in flies and humans. Northern blot analysis and epitope tagging show that the hdlc1 gene is ubiquitously expressed and that the human dynein light chain 1 is localized in the cytoplasm. hdlc1 maps to 14q24.


This article has been cited by other articles:

  • Yang, P., Yang, C., Wirschell, M., Davis, S. (2009). Novel LC8 Mutations Have Disparate Effects on the Assembly and Stability of Flagellar Complexes. J. Biol. Chem. 284: 31412-31421 [Abstract] [Full Text]  
  • Chen, Y.-M., Gerwin, C., Sheng, Z.-H. (2009). Dynein Light Chain LC8 Regulates Syntaphilin-Mediated Mitochondrial Docking in Axons. J. Neurosci. 29: 9429-9438 [Abstract] [Full Text]  
  • King, S. M. (2008). Dynein-Independent Functions of DYNLL1/LC8: Redox State Sensing and Transcriptional Control. Sci Signal 1: pe51-pe51 [Abstract] [Full Text]  
  • Mische, S., He, Y., Ma, L., Li, M., Serr, M., Hays, T. S. (2008). Dynein Light Intermediate Chain: An Essential Subunit That Contributes to Spindle Checkpoint Inactivation. Mol. Biol. Cell 19: 4918-4929 [Abstract] [Full Text]  
  • Tanner, C. A., Rompolas, P., Patel-King, R. S., Gorbatyuk, O., Wakabayashi, K.-i., Pazour, G. J., King, S. M. (2008). Three Members of the LC8/DYNLL Family Are Required for Outer Arm Dynein Motor Function. Mol. Biol. Cell 19: 3724-3734 [Abstract] [Full Text]  
  • Jung, Y., Kim, H., Min, S. H., Rhee, S. G., Jeong, W. (2008). Dynein Light Chain LC8 Negatively Regulates NF-{kappa}B through the Redox-dependent Interaction with I{kappa}B{alpha}. J. Biol. Chem. 283: 23863-23871 [Abstract] [Full Text]  
  • Song, C., Wen, W., Rayala, S. K., Chen, M., Ma, J., Zhang, M., Kumar, R. (2008). Serine 88 Phosphorylation of the 8-kDa Dynein Light Chain 1 Is a Molecular Switch for Its Dimerization Status and Functions. J. Biol. Chem. 283: 4004-4013 [Abstract] [Full Text]  
  • Lajoix, A.-D., Badiou, S., Peraldi-Roux, S., Chardes, T., Dietz, S., Aknin, C., Tribillac, F., Petit, P., Gross, R. (2006). Protein Inhibitor of Neuronal Nitric Oxide Synthase (PIN) Is a New Regulator of Glucose-Induced Insulin Secretion. Diabetes 55: 3279-3288 [Abstract] [Full Text]  
  • Wang, R.-A., Zhao, M., Meistrich, M. L., Kumar, R. (2005). Stage-specific Expression of Dynein Light Chain-1 and Its Interacting Kinase, p21-activated Kinase-1, in Rodent Testes: Implications in Spermiogenesis. J. Histochem. Cytochem. 53: 1235-1243 [Abstract] [Full Text]  
  • Havecker, E. R., Gao, X., Voytas, D. F. (2005). The Sireviruses, a Plant-Specific Lineage of the Ty1/copia Retrotransposons, Interact with a Family of Proteins Related to Dynein Light Chain 8. Plant Physiol. 139: 857-868 [Abstract] [Full Text]  
  • Ghosh-Roy, A., Desai, B. S., Ray, K. (2005). Dynein Light Chain 1 Regulates Dynamin-mediated F-Actin Assembly during Sperm Individualization in Drosophila. Mol. Biol. Cell 16: 3107-3116 [Abstract] [Full Text]  
  • Seidel, S. D., Sparrow, B. R., Kan, H.L., Stott, W. T., Schisler, M. R., Linscombe, V.A., Gollapudi, B.B. (2004). Profiles of gene expression changes in L5178Y mouse lymphoma cells treated with methyl methanesulfonate and sodium chloride. Mutagenesis 19: 195-201 [Abstract] [Full Text]  
  • Ghosh-Roy, A., Kulkarni, M., Kumar, V., Shirolikar, S., Ray, K. (2004). Cytoplasmic Dynein-Dynactin Complex Is Required for Spermatid Growth but Not Axoneme Assembly in Drosophila. Mol. Biol. Cell 15: 2470-2483 [Abstract] [Full Text]  
  • King, S. J., Brown, C. L., Maier, K. C., Quintyne, N. J., Schroer, T. A. (2003). Analysis of the Dynein-Dynactin Interaction In Vitro and In Vivo. Mol. Biol. Cell 14: 5089-5097 [Abstract] [Full Text]  
  • Wang, W., Lo, K. W.-H., Kan, H.-M., Fan, J.-S., Zhang, M. (2003). Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of the Domain-swapped Dimer Assembly. J. Biol. Chem. 278: 41491-41499 [Abstract] [Full Text]  
  • Kaiser, F. J., Tavassoli, K., Van den Bemd, G.-J., Chang, G. T.G., Horsthemke, B., Moroy, T., Ludecke, H.-J. (2003). Nuclear interaction of the dynein light chain LC8a with the TRPS1 transcription factor suppresses the transcriptional repression activity of TRPS1. Hum Mol Genet 12: 1349-1358 [Abstract] [Full Text]  
  • Kim, J. W., Wang, X. W. (2003). Gene expression profiling of preneoplastic liver disease and liver cancer: a new era for improved early detection and treatment of these deadly diseases?. Carcinogenesis 24: 363-369 [Abstract] [Full Text]  
  • Miki, F., Okazaki, K., Shimanuki, M., Yamamoto, A., Hiraoka, Y., Niwa, O. (2002). The 14-kDa Dynein Light Chain-Family Protein Dlc1 Is Required for Regular Oscillatory Nuclear Movement and Efficient Recombination during Meiotic Prophase in Fission Yeast. Mol. Biol. Cell 13: 930-946 [Abstract] [Full Text]  
  • Stamler, J. S., Meissner, G. (2001). Physiology of Nitric Oxide in Skeletal Muscle. Physiol. Rev. 81: 209-237 [Abstract] [Full Text]  
  • Chang, Y.-W. E., Jakobi, R., McGinty, A., Foschi, M., Dunn, M. J., Sorokin, A. (2000). Cyclooxygenase 2 Promotes Cell Survival by Stimulation of Dynein Light Chain Expression and Inhibition of Neuronal Nitric Oxide Synthase Activity. Mol. Cell. Biol. 20: 8571-8579 [Abstract] [Full Text]  
  • Wang, W., Chapin, R. E. (2000). Differential Gene Expression Detected by Suppression Subtractive Hybridization in the Ethylene Glycol Monomethyl Ether-Induced Testicular Lesion. Toxicol Sci 56: 165-174 [Abstract] [Full Text]  
  • Naisbitt, S., Valtschanoff, J., Allison, D. W., Sala, C., Kim, E., Craig, A. M., Weinberg, R. J., Sheng, M. (2000). Interaction of the Postsynaptic Density-95/Guanylate Kinase Domain-Associated Protein Complex with a Light Chain of Myosin-V and Dynein. J. Neurosci. 20: 4524-4534 [Abstract] [Full Text]  
  • Lizotte-Waniewski, M., Tawe, W., Guiliano, D. B., Lu, W., Liu, J., Williams, S. A., Lustigman, S. (2000). Identification of Potential Vaccine and Drug Target Candidates by Expressed Sequence Tag Analysis and Immunoscreening of Onchocerca volvulus Larval cDNA Libraries. Infect. Immun. 68: 3491-3501 [Abstract] [Full Text]  
  • Roczniak, A., Levine, D. Z., Burns, K. D. (2000). Localization of protein inhibitor of neuronal nitric oxide synthase in rat kidney. Am. J. Physiol. Renal Physiol. 278: F702-F707 [Abstract] [Full Text]  
  • Herzig, R., Andersson, U, Scarpulla, R. (2000). Dynein light chain interacts with NRF-1 and EWG, structurally and functionally related transcription factors from humans and drosophila. J. Cell Sci. 113: 4263-4273 [Abstract]  
  • Zhao, D, Clyde, D, Bownes, M (2000). Expression of fringe is down regulated by Gurken/Epidermal Growth Factor Receptor signalling and is required for the morphogenesis of ovarian follicle cells. J. Cell Sci. 113: 3781-3794 [Abstract]  
  • Bowman, A. B., Patel-King, R. S., Benashski, S. E., McCaffery, J. M., Goldstein, L. S.B., King, S. M. (1999). Drosophila roadblock and Chlamydomonas Lc7: A Conserved Family of Dynein-Associated Proteins Involved in Axonal Transport, Flagellar Motility, and Mitosis. JCB 146: 165-180 [Abstract] [Full Text]  
  • Guo, Y., Greenwood, M. T., Petrof, B. J., Hussain, S. N. A. (1999). Expression and Regulation of Protein Inhibitor of Neuronal Nitric Oxide Synthase in Ventilatory Muscles. Am. J. Respir. Cell Mol. Bio. 20: 319-326 [Abstract] [Full Text]  
  • Beckwith, S. M., Roghi, C. H., Liu, B., Ronald Morris, N. (1998). The "8-kD" Cytoplasmic Dynein Light Chain Is Required for Nuclear Migration and for Dynein Heavy Chain Localization in Aspergillus nidulans. JCB 143: 1239-1247 [Abstract] [Full Text]  
  • Pazour, G. J., Wilkerson, C. G., Witman, G. B. (1998). A Dynein Light Chain Is Essential for the Retrograde Particle Movement of Intraflagellar Transport (IFT). JCB 141: 979-992 [Abstract] [Full Text]  
  • Harrison, A., Olds-Clarke, P., King, S. M. (1998). Identification of the t Complex-encoded Cytoplasmic Dynein Light Chain Tctex1 in Inner Arm I1 Supports the Involvement of Flagellar Dyneins in Meiotic Drive. JCB 140: 1137-1147 [Abstract] [Full Text]  
  • Benashski, S. E., Harrison, A., Patel-King, R. S., King, S. M. (1997). Dimerization of the Highly Conserved Light Chain Shared by Dynein and Myosin V. J. Biol. Chem. 272: 20929-20935 [Abstract] [Full Text]  
  • Hoffmann, K. F., Strand, M. (1997). Molecular Characterization of a 20.8-kDa Schistosoma mansoni Antigen. SEQUENCE SIMILARITY TO TEGUMENTAL ASSOCIATED ANTIGENS AND DYNEIN LIGHT CHAINS. J. Biol. Chem. 272: 14509-14515 [Abstract] [Full Text]  
  • Jackson, S., Blochlinger, K (1997). cut interacts with Notch and protein kinase A to regulate egg chamber formation and to maintain germline cyst integrity during Drosophila oogenesis. Development 124: 3663-3672 [Abstract]  
  • King, S. M., Dillman, J. F. III, Benashski, S. E., Lye, R. J., Patel-King, RamilaS., Pfister, K.K. (1996). The Mouse t-Complex-encoded Protein Tctex-1 Is a Light Chain of Brain Cytoplasmic Dynein. J. Biol. Chem. 271: 32281-32287 [Abstract] [Full Text]  
  • Jaffrey, S. R., Snyder, S. H. (1996). PIN: An Associated Protein Inhibitor of Neuronal Nitric Oxide Synthase. Science 274: 774-777 [Abstract] [Full Text]  
  • King, S. M., Barbarese, E., Dillman, J. F. III, Patel-King, R. S., Carson, J. H., Pfister, K. K. (1996). Brain Cytoplasmic and Flagellar Outer Arm Dyneins Share a Highly Conserved Mr 8,000Light Chain. J. Biol. Chem. 271: 19358-19366 [Abstract] [Full Text]  
  • Phillis, R, Statton, D, Caruccio, P, Murphey, R. (1996). Mutations in the 8 kDa dynein light chain gene disrupt sensory axon projections in the Drosophila imaginal CNS. Development 122: 2955-2963 [Abstract]  
  • DiBella, L. M., Benashski, S. E., Tedford, H. W., Harrison, A., Patel-King, R. S., King, S. M. (2001). The Tctex1/Tctex2 Class of Dynein Light Chains. DIMERIZATION, DIFFERENTIAL EXPRESSION, AND INTERACTION WITH THE LC8 PROTEIN FAMILY. J. Biol. Chem. 276: 14366-14373 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»